GAS 6 (GAS6) (NM_000820) Human Untagged Clone

CAT#: SC119633

GAS6 (untagged)-Human growth arrest-specific 6 (GAS6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GAS6
• Synonyms: AXLLG; AXSF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119633 sequence for NM_000820 edited (data generated by NextGen Sequencing)
  ATGGCCCCTTCGCTCTCGCCCGGGCCCGCCGCCCTGCGCCGCGCGCCGCAGCTGCTGCTG
  CTGCTGCTGGCCGCGGAGTGCGCGCTTGCCGCGCTGTTGCCGGCGCGCGAGGCCACGCAG
  TTCCTGCGGCCCAGGCAGCGCCGCGCCTTTCAGGTCTTCGAGGAGGCCAAGCAGGGCCAC
  CTGGAGAGGGAGTGCGTGGAGGAGCTGTGCAGCCGCGAGGAGGCGCGGGAGGTGTTCGAG
  AACGACCCCGAGACGGATTATTTTTACCCAAGATACTTAGACTGCATCAACAAGTATGGG
  TCTCCGTACACCAAAAACTCAGGCTTCGCCACCTGCGTGCAAAACCTGCCTGACCAGTGC
  ACGCCCAACCCCTGCGATAGGAAGGGGACCCAAGCCTGCCAGGACCTCATGGGCAACTTC
  TTCTGCCTGTGTAAAGCTGGCTGGGGGGGCCGGCTCTGCGACAAAGATGTCAACGAATGC
  AGCCAGGAGAACGGGGGCTGCCTCCAGATCTGCCACAACAAGCCGGGTAGCTTCCACTGT
  TCCTGCCACAGCGGCTTCGAGCTCTCCTCTGATGGCAGGACCTGCCAAGACATAGACGAG
  TGCGCAGACTCGGAGGCCTGCGGGGAGGCGCGCTGCAAGAACCTGCCCGGCTCCTACTCC
  TGCCTCTGTGACGAGGGCTTTGCGTACAGCTCCCAGGAGAAGGCTTGCCGAGATGTGGAC
  GAGTGTCTGCAGGGCCGCTGTGAGCAGGTCTGCGTGAACTCCCCAGGGAGCTACACCTGC
  CACTGTGACGGGCGTGGGGGCCTCAAGCTGTCCCAGGACATGGACACCTGTGAGGACATC
  TTGCCGTGCGTGCCCTTCAGCGTGGCCAAGAGTGTGAAGTCCTTGTACCTGGGCCGGATG
  TTCAGTGGGACCCCCGTGATCCGACTGCGCTTCAAGAGGCTGCAGCCCACCAGGCTGGTA
  GCTGAGTTTGACTTCCGGACCTTTGACCCCGAGGGCATCCTCCTCTTTGCCGGAGGCCAC
  CAGGACAGCACCTGGATCGTGCTGGCCCTGAGAGCCGGCCGGCTGGAGCTGCAGCTGCGC
  TACAACGGTGTCGGCCGTGTCACCAGCAGCGGCCCGGTCATCAACCATGGCATGTGGCAG
  ACAATCTCTGTTGAGGAGCTGGCGCGGAATCTGGTCATCAAGGTCAACAGGGATGCTGTC
  ATGAAAATCGCGGTGGCCGGGGACTTGTTCCAACCGGAGCGAGGACTGTATCATCTGAAC
  CTGACCGTGGGAGGTATTCCCTTCCATGAGAAGGACCTCGTGCAGCCTATAAACCCTCGT
  CTGGATGGCTGCATGAGGAGCTGGAACTGGCTGAACGGAGAAGACACCACCATCCAGGAA
  ACGGTGAAAGTGAACACGAGGATGCAGTGCTTCTCGGTGACGGAGAGAGGCTCTTTCTAC
  CCCGGGAGCGGCTTCGCCTTCTACAGCCTGGACTACATGCGGACCCCTCTGGACGTCGGG
  ACTGAATCAACCTGGGAAGTAGAAGTCGTGGCTCACATCCGCCCAGCCGCAGACACAGGC
  GTGCTGTTTGCGCTCTGGGCCCCCGACCTCCGTGCCGTGCCTCTCTCTGTGGCACTGGTA
  GACTATCACTCCACGAAGAAACTCAAGAAGCAGCTGGTGGTCCTGGCCGTGGAGCATACG
  GCCTTGGCCCTAATGGAGATCAAGGTCTGCGACGGCCAAGAGCACGTGGTCACCGTCTCG
  CTGAGGGACGGTGAGGCCACCCTGGAGGTGGACGGCACCAGGGGCCAGAGCGAGGTGAGC
  GCCGCGCAGCTGCAGGAGAGGCTGGCCGTGCTCGAGAGGCACCTGCGGAGCCCCGTGCTC
  ACCTTTGCTGGCGGCCTGCCAGATGTGCCGGTGACTTCAGCGCCAGTCACCGCGTTCTAC
  CGCGGCTGCATGACACTGGAGGTCAACCGGAGGCTGCTGGACCTGGACGAGGCGGCGTAC
  AAGCACAGCGACATCACGGCCCACTCCTGCCCCCCCGTGGAGCCCGCCGCAGCCTAG
  
  Clone variation with respect to NM_000820.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000820 unedited
  GCAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCGCCAGNA
  CGGGATGACCGGAGCCTCCGCCCCGCGGCGCCCGCGGCTCGCCTCGGCCTCCCGGGCGCT
  CTGACCGCGCGTCCCCGGCCCGCCATGGCCCCTTCGCTCTCGCCCGGGCCCGCCGCCCTG
  CGCCGCGCGCCGCAGCTGCTGCTGCTGCTGCTGGCCGCGGAGTGCGCGCTTGCCGCGCTG
  TTGCCGGCGCGCGAGGCCACGCAGTTCCTGCGGCCCAGGCAGCGCCGCGCCTTTCAGGTC
  TTCGAGGAGGCCAAGCAGGGCCACCTGGAGAGGGAGTGCGTGGAGGAGCTGTGCAGCCGC
  GAGGAGGCGCGGGAGGTGTTCGAGAACGACCCCGAGACGGATTATTTTTACCCAAGATAC
  TTAGACTGCATCAACAAGTATGGGTCTCCGTACACCAAAAACTCAGGCTTCGCCACCTGC
  GTGCAAAACCTGCCTGACCAGTGCACGCCCAACCCCTGCGATAGGAAGGGGACCCAAGCC
  TGCCAGGACCTCATGGGCAACTTCTTCTGCCTGTGTAAAGCTGGCTGGGGGGGCCGGCTC
  TGCGACAAAGATGTCAACGAATGCAGCCAGGAGAACGGNGGCTGCCTCCAGATCTGCCAC
  AACAAGCCGGGTAGCTTCCACTGTTCCTGCCACAGCGGCTTCGAGCTCTCCTCTGATGGC
  AGGACCTGCCAAGACATANACGAGTGCGCAGAANNTCGAGCCTGNCGGGGAGGCGCGCTG
  CAAGAACCTGCCCGGGGCTCTACTCTGCCTCTGGGACGAAGGGCTNTGCGTACAGCTCCC
  AAGANAAGGCTGCCCGAATGTGGAACAATGTCCTGCAGGGCCCTGGGAAACCAGTTCTGC
  GTGAACTCCCCAGNAGCTACACTGCCTTGGACCGGGGGGGTGGGGGGGGCTTCAAGCTGT
  CCCAGAACTGACCCCTCTGTGAGGGTACTGNGCCCGGGCTGCCCTCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000820 unedited
  NNNTTTGCTCTGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACAAACCTTTTGGAAAATATTTTATTT
  TCCATATTTAAAAGCCAAAAAAAAGCGCTGGGTAATAAAAATAATAGAAAATTTTTTTTT
  TCAACCCCGTTTTGGGCTGGGCCGGCCTCCCCGGGCCCGGGCCCACGGTTGAGGGCGCGG
  GGTAAAAGGCCCCAAGCCCATTTCACTTTTTACAGATTTGTTACAGGCCGGGAGGGTCAC
  AAAGGAAAGCCCAGTTTTAAACAGGGCCCCACGGGGGAGGAGGGAGCCCCCAGGCTCCTC
  CCGGTTGTTTGGGACAAAAACTGAAAACCCTGCCGGGTCCCGGGGGGGCCTAGGCTGGGG
  GGGGCTCCACGGGGGGGCAGGAGGGGGCCGTGATGTCCTTGTGCTTGTACCCCGCCTTGT
  CCAGGTCCAGAAGCCTCCGGTTGACCTCCAGGGTCATGCACCCGCGGAAAAACGCGGGGA
  CTGGCGCTAAAGTCACCGGCACATTTGGCAGGCCGCCAACAAAGGGGAGCACGGGGCTCC
  GAAGGGGCCTTTAGAGCACGGCCAGCCTTTCCTGGAGTTGGGGGGGGCTCACCTCGTTTT
  GGCCCCTGGGGGCCGTCCCCTCCCAGGGGGGCCTCACCGTCCCTCAGGGAGAACGGGGAC
  CACGTGCTCTTTGGCCGGGCGCAAACCTTTGATCTTCCATTAGGGGCAAAGGCCGTATTG
  CTCCACGGCCAAGGACCACCAGCGTGTTTTTTGAAGTTCTTTCGGGGGAGAGAAAGCCAA
  CCCGGGCCCAAAAAAAAGGCCGCCCCGGAGGTCGGGGGGCCCAAAGCGAAAAAACCCCCC
  TGGTTTGGGGTTGGGCGATGGTACACCCCACTCCCCCTCCCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000820
• Insert Size: 2550 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000820.1, NP_000811.1
• RefSeq Size: 2461 bp
• RefSeq ORF: 2037 bp
• Locus ID: 2621
• Cytogenetics: 13q34
• Domains: GLA, LamG, EGF_CA, EGF, EGF
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene encodes a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation. This gene is frequently overexpressed in many cancers and has been implicated as an adverse prognostic marker. Elevated protein levels are additionally associated with a variety of disease states, including venous thromboembolic disease, systemic lupus erythematosus, chronic renal failure, and preeclampsia. [provided by RefSeq, Aug 2014]'