Ionotropic Glutamate receptor 2 (GRIA2) (NM_000826) Human Untagged Clone

CAT#: SC119637

GRIA2 (untagged)-Human glutamate receptor, ionotropic, AMPA 2 (GRIA2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRIA2
• Synonyms: GluA2; gluR-2; gluR-B; GluR-K2; GLUR2; GLURB; HBGR2; NEDLIB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000826 edited
  CTGGGAAATAGGGATTCTTCTGCCTCCACTTCAGGTTTTAGCAGCTTGGTGCTAAATTGC
  TGTCTCAAAATGCAGAGGATCTAATTTGCAGAGGAAAACAGCCAAAGAAGGAAGAGGAGG
  AAAAGGAAAAAAAAAGGGGTATATTGTGGATGCTCTACTTTTCTTGGAAATGCAAAAGAT
  TATGCATATTTCTGTCCTCCTTTCTCCTGTTTTATGGGGACTGATTTTTGGTGTCTCTTC
  TAACAGCATACAGATAGGGGGGCTATTTCCTAGGGGCGCCGATCAAGAATACAGTGCATT
  TCGAGTAGGGATGGTTCAGTTTTCCACTTCGGAGTTCAGACTGACACCCCACATCGACAA
  TTTGGAGGTGGCAAACAGCTTCGCAGTCACTAATGCTTTCTGCTCCCAGTTTTCGAGAGG
  AGTCTATGCTATTTTTGGATTTTATGACAAGAAGTCTGTAAATACCATCACATCATTTTG
  CGGAACACTCCACGTCTCCTTCATCACTCCCAGCTTCCCAACAGATGGCACACATCCATT
  TGTCATTCAGATGAGACCCGACCTCAAAGGAGCTCTCCTTAGCTTGATTGAATACTATCA
  ATGGGACAAGTTTGCATACCTCTATGACAGTGACAGAGGCTTATCAACACTGCAAGCTGT
  GCTGGATTCTGCTGCTGAAAAGAAATGGCAAGTGACTGCTATCAATGTGGGAAACATTAA
  CAATGACAAGAAAGATGAGATGTACCGATCACTTTTTCAAGATCTGGAGTTAAAAAAGGA
  ACGGCGTGTAATTCTGGACTGTGAAAGGGATAAAGTAAACGACATTGTAGACCAGGTTAT
  TACCATTGGAAAACACGTTAAAGGGTACCACTACATCATTGCAAATCTGGGATTTACTGA
  TGGAGACCTATTAAAAATCCAGTTTGGAGGTGCAAATGTCTCTGGATTTCAGATAGTGGA
  CTATGATGATTCGTTGGTATCTAAATTTATAGAAAGATGGTCAACACTGGAAGAAAAAGA
  ATACCCTGGAGCTCACACAACAACAATTAAGTATACTTCTGCTCTGACCTATGATGCCGT
  TCAAGTGATGACTGAAGCCTTCCGCAACCTAAGGAAGCAAAGAATTGAAATCTCCCGAAG
  GGGGAATGCAGGAGACTGTCTGGCAAACCCAGCAGTGCCCTGGGGACAAGGTGTAGAAAT
  AGAAAGGGCCCTCAAACAGGTTCAGGTTGAAGGTCTCTCAGGAAATATAAAGTTTGACCA
  GAATGGAAAAAGAATAAACTATACAATTAACATCATGGAGCTCAAAACTAATGGGCCCCG
  GAAGATTGGCTACTGGAGTGAAGTGGACAAAATGGTTGTTACCCTTACTGAGCTCCCTTC
  TGGAAATGACACCTCTGGGCTTGAGAATAAGACTGTTGTTGTCACCACAATTTTGGAATC
  TCCGTATGTTATGATGAAGAAAAATCATGAAATGCTTGAAGGCAATGAGCGCTATGAGGG
  CTACTGTGTTGACCTGGCTGCAGAAATCGCCAAACATTGTGGGTTCAAGTACAAGTTGAC
  AATTGTTGGTGATGGCAAGTATGGGGCCAGGGATGCAGACACGAAAATTTGGAATGGGAT
  GGTTGGAGAACTTGTATATGGGAAAGCTGATATTGCAATTGCTCCATTAACTATTACCCT
  TGTGAGAGAAGAGGTGATTGACTTCTCAAAGCCCTTCATGAGCCTCGGGATATCTATCAT
  GATCAAGAAGCCTCAGAAGTCCAAACCAGGAGTGTTTTCCTTTCTTGATCCTTTAGCCTA
  TGAGATCTGGATGTGCATTGTTTTTGCCTACATTGGGGTCAGTGTAGTTTTATTCCTGGT
  CAGCAGATTTAGCCCCTACGAGTGGCACACTGAGGAGTTTGAAGATGGAAGAGAAACACA
  AAGTAGTGAATCAACTAATGAATTTGGGATTTTTAATAGTCTCTGGTTTTCCTTGGGTGC
  CTTTATGCGGCAGGGATGCGATATTTCGCCAAGATCCCTCTCTGGGCGCATTGTTGGAGG
  TGTGTGGTGGTTCTTTACCCTGATCATAATCTCCTCCTACACGGCTAACTTAGCTGCCTT
  CCTGACTGTAGAGAGGATGGTGTCTCCCATCGAAAGTGCTGAGGATCTTTCTAAGCAAAC
  AGAAATTGCTTATGGAACATTAGACTCTGGCTCCACTAAAGAGTTTTTCAGGAGATCTAA
  AATTGCAGTGTTTGATAAAATGTGGACCTACATGCGGAGTGCGGAGCCCTCTGTGTTTGT
  GAGGACTACGGCCGAAGGGGTGGCTAGAGTGCGGAAGTCCAAAGGGAAATATGCCTACTT
  GTTGGAGTCCACGATGAACGAGTACATTGAGCAAAGGAAGCCTTGCGACACCATGAAAGT
  TGGTGGAAACCTGGATTCCAAAGGCTATGGCATCGCAACACCTAAAGGATCCTCATTAAG
  AACCCCAGTAAATCTTGCAGTATTGAAACTCAGTGAGCAAGGCGTCTTAGACAAGCTGAA
  AAACAAATGGTGGTACGATAAAGGTGAATGTGGAGCCAAGGACTCTGGAAGTAAGGAAAA
  GACCAGTGCCCTCAGTCTGAGCAACGTTGCTGGAGTATTCTACATCCTTGTCGGGGGCCT
  TGGTTTGGCAATGCTGGTGGCTTTGATTGAGTTCTGTTACAAGTCAAGGGCCGAGGCGAA
  ACGAATGAAGGTGGCAAAGAATGCACAGAATATTAACCCATCTTCCTCGCAGAATTCACA
  GAATTTTGCAACTTATAAGGAAGGTTACAACGTATATGGCATCGAAAGTGTTAAAATTTA
  GGGGATGACCTTGAATGATGCCATGAGGAACAAGGCAAGGCTGTCAATTACAGGAAGTAC
  TGGAGAAAATGGACGTGTTATGACTCCAGAATTTCCCAAAGCAGTGCATGCTGTCCCTTA
  CGTGAGTCCTGGCATGGGAATGAATGTCAGTGTGACTGATCTCTCGTGATTGATAAGAAC
  CTTTTGAGTGCCTTACACAATGGTTTTCTTGTGTGTTTATTGTCAAAGTGGTGAGAGGCA
  TCCAGTATCTTGAAGACTTTTCTTTCAGCCAAGAATTCTTAAATATGTGGAGTTCATCTT
  GAATTGTAAGGAATGATTAATTAAAACACAACATCTTTTTCTACTCGAGTTACAGACAAA
  GCGTGGTGGACATGCACAGCTAACATGGAAGTACTATAATTTACCTGAAGTCTTTGTACA
  GACAACAAACCTGTTTCTGCAGCCACTATTGTTAGTCTCTTGATTCATAATGACTTAAGC
  ACACTTGACATCAACTGCATCAAGATGTGACATGTTTTATAAAAAAAGGAAAAAAAAACT
  AGAC
• Restriction Sites: NotI-NotI
• ACCN: NM_000826
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_000826.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000826.2, NP_000817.2
• RefSeq Size: 5644 bp
• RefSeq ORF: 2652 bp
• Locus ID: 2891
• Cytogenetics: 4q32.1
• Domains: lig_chan, ANF_receptor
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Long-term depression, Long-term potentiation, Neuroactive ligand-receptor interaction
• Gene Summary: 'Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes isoform 1 (also known as flip isoform). RNA editing (CAG->CGG) changes aa Gln607Arg. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript matching the reference genomic sequence was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.