GST3 (GSTP1) (NM_000852) Human Untagged Clone

CAT#: SC119655

GSTP1 (untagged)-Human glutathione S-transferase pi 1 (GSTP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GSTP1
• Synonyms: DFN7; FAEES3; GST3; GSTP; HEL-S-22; PI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119655 sequence for NM_000852 edited (data generated by NextGen Sequencing)
  ATGCCGCCCTACACCGTGGTCTATTTCCCAGTTCGAGGCCGCTGCGCGGCCCTGCGCATG
  CTGCTGGCAGATCAGGGCCAGAGCTGGAAGGAGGAGGTGGTGACCGTGGAGACGTGGCAG
  GAGGGCTCACTCAAAGCCTCCTGCCTATACGGGCAGCTCCCCAAGTTCCAGGACGGAGAC
  CTCACCCTGTACCAGTCCAATACCATCCTGCGTCACCTGGGCCGCACCCTTGGGCTCTAT
  GGGAAGGACCAGCAGGAGGCAGCCCTGGTGGACATGGTGAATGACGGCGTGGAGGACCTC
  CGCTGCAAATACATCTCCCTCATCTACACCAACTATGAGGCGGGCAAGGATGACTATGTG
  AAGGCACTGCCCGGGCAACTGAAGCCTTTTGAGACCCTGCTGTCCCAGAACCAGGGAGGC
  AAGACCTTCATTGTGGGAGACCAGATCTCCTTCGCTGACTACAACCTGCTGGACTTGCTG
  CTGATCCATGAGGTCCTAGCCCCTGGCTGCCTGGATGCGTTCCCCCTGCTCTCAGCATAT
  GTGGGGCGCCTCAGTGCCCGGCCCAAGCTCAAGGCCTTCCTGGCCTCCCCTGAGTACGTG
  AACCTCCCCATCAATGGCAACGGGAAACAGTGA
  
  Clone variation with respect to NM_000852.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000852 unedited
  CACGAGGGCTCGGAGGCCGCGAGGCCTTCGCTGGAGTTTCGCCGCCGCAGTCTTCGCCAC
  CATGCCGCCCTACACCGTGGTCTATTTCCCAGTTCGAGGCCGCTGCGCGGCCCTGCGCAT
  GCTGCTGGCAGATCAGGGCCAGAGCTGGAAGGAGGAGGTGGTGACCGTGGAGACGTGGCA
  GGAGGGCTCACTCAAAGCCTCCTGCCTATACGGGCAGCTCCCCAAGTTCCAGGACGGAGA
  CCTCACCCTGTACCAGTCCAATACCATCCTGCGTCACCTGGGCCGCACCCTTGGGCTCTA
  TGGGAAGGACCAGCAGGAGGCAGCCCTGGTGGACATGGTGAATGACGGCGTGGAGGACCT
  CCGCTGCAAATACATCTCCCTCATCTACACCAACTATGAGGCGGGCAAGGATGACTATGT
  GAAGGCACTGCCCGGGCAACTGAAGCCTTTTGAGACCCTGCTGTCCCAGAACCAGGGAGG
  CAAGACCTTCATTGTGGGAGACCAGATCTCCTTCGCTGACTACAACCTGCTGGACTTGCT
  GCTGATCCATGAGGTCCTAGCCCCTGGCTGCCTGGATGCGTTCCCCCTGCTCTCAGCATA
  TGTGGGGCGCCTCAGTGCCCGGCCCAAGCTCAAGGCCTTCCTGGCCTCCCCTGAGTACGT
  GAACCTCCCCATCAATGGCAACGGGAAACAGTGAGGGTTGGGGGGACTCTGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000852 unedited
  CGCGGGCCGCAATCTANAATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTAACTCTCTTAAAAATTTATTGGTCCTGGAAAAAGGAAGGG
  AAACTTTGCCTCCCGCTTAAAATCCCCCCAACCCTTACTGTTTCCCGTTGCCATTGATGG
  GGAGGTTCACGTACTCAGGGGAGGCCAAAAAGGCCTTGAACTTGGGCCGGGCACTGAGGC
  CCCCCACATTTTCTGAAAACAGGGGGAACCCATCCAGGCACCCAGGGGCTAGGACCTCAT
  GGATCAACAGCAAGTCCAACAGGTTGTAATCAACGAAAGAAATCTGGTCTCCCACAATGA
  AAGGCTTGCCTCCCTGGTTCTGGGACAACAGGGTCTCAAAAGGGTTTAATTGCCCGGGCA
  GGGCCTTCACATAATCATCCTTGCCCCCCTCATAATTGGGGTAAATGAGGGAAAAGTATT
  TGCAGCGGAGGGCCTCCACCCCGTCATTCACCATGTCCACCAGGGCTGCCTCCTGCTGGT
  CCTTCCCATAAAACCCAAGGGTGCGGCCCAAGTGACCCAAGATGGTATTGGACTGGTACA
  GGGTGAGGGCTCCGTCCTGGACTTGGGGAGCTGCCCGTAAGGCAAGAAGCTTTGAATGAG
  CCCTTCTGGCACGTTTCCACGGGTAACCACCTCTCTTCCAATTTGGGCCTGGACTGCCAA
  CAAATGCCCAAGGGCCCCCAAGGCCTTAAACTGGGAATAAAACACGGGTAAGGCGGCATG
  TTGCAAAAACTTCGCGGGGAAACTCAAGAAAGGCCTCGGGCCCCAAACCTGTGCCAATTA
  GGGGCCCCCTTATGGAGCTATACAAAATTTGACGGTCACTAAAAACTTTGTTAAAGAACC
  TCCCGGCACCCCACCCCATTGGGCACGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000852
• Insert Size: 870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000852.2, NP_000843.1
• RefSeq Size: 737 bp
• RefSeq ORF: 633 bp
• Locus ID: 2950
• Cytogenetics: 11q13.2
• Domains: GST_N, GST_C
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450, Pathways in cancer, Prostate cancer
• Gene Summary: 'Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]'