Glutathione S Transferase theta 2 (GSTT2) (NM_000854) Human Untagged Clone

CAT#: SC119657

GSTT2 (untagged)-Human glutathione S-transferase theta 2 (GSTT2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GSTT2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000854, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCCTAGAGCTGTTTCTTGACCTGGTGTCCCAGCCCAGCCGCGCCGTCTACATCTTCGCCAAGAAGA
  ATGGCATCCCCTTAGAGCTGCGCACCGTGGATTTGGTCAAAGGGCAGCACAAGAGCAAGGAGTTCTTGCA
  GATCAACAGCCTGGGGAAACTGCCGACGCTCAAGGATGGTGATTTCATCTTGACCGAAAGCTCGGCCATC
  CTGATTTACCTGAGCTGTAAGTACCAGACGCCGGACCACTGGTATCCATCTGACCTGCAGGCTCGTGCCC
  GTGTTCATGAGTACCTGGGCTGGCATGCCGACTGCATCCGTGGCACCTTTGGTATACCCCTGTGGGTCCA
  GGTGTTGGGGCCACTCATTGGGGTCCAGGTGCCCGAGGAGAAGGTGGAACGCAACAGGACTGCCATGGAC
  CAGGCCCTGCAATGGCTGGAGGACAAGTTCCTGGGGGACAGGCCCTTCCTCGCTGGCCAGCAGGTGACAC
  TGGCTGATCTCATGGCCCTGGAGGAGCTGATGCAGCCGGTGGCTCTCGGCTATGAACTGTTTGAGGGACG
  GCCACGACTGGCAGCATGGCGTGGACGAGTGGAGGCTTTCCTGGGTGCTGAGCTATGCCAGGAGGCCCAC
  AGCATCATCTTGAGCATCCTGGAACAGGCGGCCAAGAAAACCCTCCCAACACCCTCACCAGAGGCCTATC
  AGGCTATGCTGCTTCGAATCGCCAGGATCCCCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000854 unedited
  ATTTGTAATACGACTCACTATAGAGGCGGCCGCGATTCGGCACGAGGCCGCGCTCAGCGC
  CTTCACTGCCATCCCCGCTGTCCTTGCCGCCCCCGCCATGGGCCTAGAGCTGTTTCTTGA
  CCTGGTGTCCCAGCCCAGCCGCGCCGTCTACATCTTCGCCAAGAAGAATGGCATCCCCTT
  AGAGCTGCGCACCGTGGATTTGGTCAAAGGGCAGCACAAGAGCAAGGAGTTCTTGCAGAT
  CAACAGCCTGGGGAAACTGCCGACGCTCAAGGATGGTGATTTCATCTTGACCGAAAGCTC
  GGCCATCCTGATTTACCTGAGCTGTAAGTACCAGACGCCGGACCACTGGTATCCATCTGA
  CCTGCAGGCTCGTGCCCGTGTTCATGAGTACCTGGGCTGGCATGCCGACTGCATCCGTGG
  CACCTTTGGTATACCCCTGTGGGTCCAGGTGTTGGGGCCACTCATTGGGGTCCAGGTGCC
  CGAGGAGAAGGTGGAACGCAACAGGACTGCCATGGACCAGGCCCTGCAATGGCTGGAGGA
  CAAGTTCCTGAGGAACAGGCCCTTCCTCGCTGGCCAGCAGGTGACACTGGCTGATCTCAT
  GGCCCTGGAGGAGCTGATGCAGCCGGTGGCTCTCGGCTATGAACTGTTTGAGGGACGGCC
  ACGACTGGCAGCATGGCGTGGACGAGTGGAGGCTTTCCTGGGTGCTGAGCTATGCCAGNA
  GGCCCACAGCATCATCTTGAGCATCCTGGAACAGGCGGNCAAGATAACCCTCCCCACACC
  CTCACCAGAGGCCTATCAGGCTATGCTGCTTTCGATCGGCAAGATCCCCTGAAGGGTCTG
  GATTGGGGGGCCCAGAAATANCACCAGGATTCATTCTGNTACTTACTTGGCCCCTTTTAT
  CTTTCCCTTTGGCCCAGNCCTTTCTCTCAACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000854 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTATTCAAAGTGTTTGG
  AATCTTTATTCTGTTTAAAACACTGATGACATTTGCCAATTTTTGAGTGTTCTTCATGTT
  ACTGATTTGTAGGAGCTCTTTATATATTGCAGATTTAGTCTCTTACTGGTTATGTATGCT
  GCACCTGAGGAGTAGAAGCACTGCCAAGGACACTGAGTGTCTTGTCTGTGCAGAGCTTCA
  CATGAAGCTGGAGAGAAGGGACTGGGGCAAGAGGGAAAGATAAAAAGGGGCAAGTAAGTA
  ACAGAATGAATCCTTGTTGCTAATCTCCTGGCCCCCATCCCAGACCCTTCAGGGGATCCT
  GGCGATTCGAAGCAGCATAGCCTGATAGGCCTCTGGTGAGGGTGTTGGGAGGGTTTTCTT
  GGCCGCCTGTTCCAGGATGCTCAAGATGATGCTGTGGGCCTCCTGGCATAGCTCAGCACC
  CAGGAAAGCCTCCACTCGTCCACGCCATGCTGCCAGTCGTGGCCGTCCCTCAAACAGTTC
  ATACCCGAGAGCCACCGGCTGCATCACCTCCTCCAGGGCCATGAGATCAGCCAGTGTCAC
  CTGCTGGCCCACGAGGAAGGGCCTGTTCCCCAGGAACTTGTCCTTCAGCCATTGCAGGGC
  CTGGCCCATGGCAGTCCTGTTGCGTCTCACCTCTCCTCCGGCACCTGGACCCCATGAGTG
  GCCCCAACACCTGGACCCACAGGGGTATACCAAAAGTGCCCCGATGCATTCCGCATGCCA
  CCCACGCCACTATGACAAGGGCCCGACCCTGCCAGCCATCACGAAACCAGTTGTCCCGGN
  GCCGGCACTTACCGCTAATGCAATCATGATGCGCCGCCTTTGGCCACATGACACACCATC
  TTGACCCCCGCCGTCTCCTAGCTGTCGATTTGAAAACTCCTGTTTTGGGCGGCCTTCACA
  AAACCAGGCGCCCCTTTAAGGCAGCCCTTCCCTGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000854
• Insert Size: 1170 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000854.2, NP_000845.1
• RefSeq Size: 1100 bp
• RefSeq ORF: 735 bp
• Locus ID: 2953
• Cytogenetics: 22q11.23
• Domains: GST_N, GST_C
• Protein Pathways: Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450
• Gene Summary: 'The protein encoded by this gene, glutathione S-transferase (GST) theta 2 (GSTT2), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT2 and GSTT2B are nearly identical to each other, and share 55% amino acid identity with GSTT1. All three genes may play a role in human carcinogenesis. The GSTT2 gene is a pseudogene in some populations. [provided by RefSeq, Sep 2015]'
  Transcript Variant: This variant (1, coding) encodes the longer isoform (a). This variant represents the ALT_REF_LOCI_1 alternate haplotype in the GRCh38 reference genome assembly.