Prostaglandin dehydrogenase 1 (HPGD) (NM_000860) Human Untagged Clone

CAT#: SC119661

HPGD (untagged)-Human hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HPGD
• Synonyms: 15-PGDH; PGDH; PGDH1; PHOAR1; SDR36C1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119661 sequence for NM_000860 edited (data generated by NextGen Sequencing)
  ATGCACGTGAACGGCAAAGTGGCGCTGGTGACCGGCGCGGCTCAGGGCATAGGCAGAGCC
  TTTGCAGAGGCGCTGCTGCTTAAGGGCGCCAAGGTAGCGCTGGTGGATTGGAATCTTGAA
  GCAGGTGTACAGTGTAAAGCTGCCCTGGATGAGCAATTTGAACCTCAGAAGACTCTGTTC
  ATCCAGTGCGATGTGGCTGACCAGCAACAACTGAGAGACACTTTTAGAAAAGTTGTAGAC
  CACTTTGGAAGACTGGACATTTTGGTCAATAATGCTGGAGTGAATAATGAGAAAAACTGG
  GAAAAAACTCTGCAAATTAATTTGGTTTCTGTTATCAGTGGAACCTATCTTGGTTTGGAT
  TACATGAGTAAGCAAAATGGAGGTGAAGGCGGCATCATTATCAATATGTCATCTTTAGCA
  GGACTCATGCCCGTTGCACAGCAGCCGGTTTATTGTGCTTCAAAGCATGGCATAGTTGGA
  TTCACACGCTCAGCAGCGTTGGCTGCTAATCTTATGAACAGTGGTGTGAGACTGAATGCC
  ATTTGTCCAGGCTTTGTTAACACAGCCATCCTTGAATCAATTGAAAAAGAAGAAAACATG
  GGACAATATATAGAATATAAGGATCATATCAAGGATATGATTAAATACTATGGAATTTTG
  GACCCACCATTGATTGCCAATGGATTGATAACACTCATTGAAGATGATGCTTTAAATGGT
  GCTATTATGAAGATCACAACTTCTAAGGGAATTCATTTTCAAGACTATGATACAACTCCA
  TTTCAAGCAAAAACCCAATGA
  
  Clone variation with respect to NM_000860.4
  156 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000860 unedited
  AGGGATTAGGTACTCCGACTTTATGATAGGNCGGAAACGCANATTCGGCACGAGCCCCAG
  CAGTGTGTTGCACCATGCACGTGAACGGCAAAGTGGCGCTGGTGACCGGCGCGGCTCAGG
  GCATAGGCAGAGCCTTTGCAGAGGCGCTGCTGCTTAAGGGCGCCAAGGTAGCGCTGGTGG
  ATTGGAATCTTGAAGCAGGTGTACAGTGTAAAGTTGCCTTTGTTGAGCAATTTGAACCTC
  ATAAGACTCTGTTCATCCAGTGCGATGTGGCTGACCAGCAACAACTGAGAGACACTTTTA
  GAAAAGTTGTAGACCACTTTGGAAGACTGGACATTTTGGTCAATAATGCTGGAGTGAATA
  ATGAGAAAAACTGGGAAAAAACTCTGCAAATTAATTTGGTTTCTGTTATCAGTGGAACCT
  ATCTTGGTTTGGATTACATGAGTAAGCAAAATGGAGGTGAAGGCGGCATCATTATCAATA
  TGTCATCTTTAGCAGGACTCATGCCCGTTGCACAGCAGCCGGTTTATTGTGCTTCAAAGC
  ATGGCATAGTTGGATTCACACGCTCAGCAGCGTTGGCTGCTAATCTTATGAACAGTGGTG
  TGAGACTGAATGCCATTTGTCCAGGCTTTGTTAACACAGCCATCCTTTGATCAATTGAAA
  AAGAAGAAAACATGGGACAATATATAGAATATAAGGGTCATATCCAGGATATGATTAAAT
  ACTATGGAATTTTGGGACCCACCATTGATTGCCAATGGATTGATAACACTCATTTGAAGA
  TGATGGCTTTAAATGGTGCTATTATGAAAATTCACACTTCTTAAGGAATTCATTTTNCAG
  ACTATGATACAACTCCTTTTCAAGCAAAAACCCCATGAACAGCTTATGTTGTTACCATAG
  CTGGAAAATAGCCAAATAGCTTTATTCAGATCCTACCTCCATTGAAATAGCTTTAAAGAA
  GTTACAATTGCAGTTTCTCCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000860
• Insert Size: 2750 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000860.3, NP_000851.2
• RefSeq Size: 2592 bp
• RefSeq ORF: 801 bp
• Locus ID: 3248
• Cytogenetics: 4q34.1
• Domains: adh_short
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).