CYP4B1 (NM_000779) Human Untagged Clone

CAT#: SC119683

CYP4B1 (untagged)-Human cytochrome P450, family 4, subfamily B, polypeptide 1 (CYP4B1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP4B1
• Synonyms: CYPIVB1; P-450HP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene SC119683 ORF sequence for NM_000779, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGCCCAGCTTCCTCTCCCTGAGCTTCTCCTCCTTGGGCCTGTGGGCTTCTGGGCTGATCTTGGTCT
  TAGGCTTTCTCAAGCTCATCCACCTGCTGCTGCGGAGGCAGACGTTGGCTAAGGCTATGGACAAATTCCC
  AGGGCCTCCCACCCACTGGCTTTTTGGACATGCCCTCGAGATCCAGGAGACGGGGAGCCTGGACAAAGTG
  GTGTCCTGGGCCCACCAGTTCCCGTATGCCCACCCACTCTGGTTCGGACAGTTCATTGGCTTCCTGAACA
  TCTATGAGCCTGACTATGCCAAAGCTGTGTACAGCCGTGGGGACCCTAAGGCCCCTGATGTGTATGACTT
  CTTCCTCCAGTGGATTGGGAGAGGCCTGCTGGTTCTTGAGGGGCCCAAGTGGTTGCAGCACCGCAAGCTG
  CTCACACCTGGCTTTCATTATGATGTGCTGAAGCCCTATGTGGCCGTGTTCACTGAGTCTACACGTATCA
  TGCTGGACAAGTGGGAAGAGAAAGCTCGGGAGGGTAAGTCCTTTGACATCTTCTGCGATGTGGGTCACAT
  GGCGCTGAACACACTCATGAAGTGCACCTTTGGAAGAGGAGACACCGGCCTGGGCCACAGGGACAGCAGC
  TACTACCTTGCAGTCAGCGATCTCACTCTGTTGATGCAGCAGCGCCTTGTGTCCTTCCAGTACCATAATG
  ACTTCATCTACTGGCTCACCCCACATGGCCGCCGCTTCCTGCGGGCCTGCCAGGTGGCCCATGACCATAC
  AGACCAGGTCATCAGGGAGCGGAAGGCAGCCCTGCAGGATGAGAAGGTGCGGAAGAAGATCCAGAACCGG
  AGGCACCTGGACTTCCTGGACATTCTCCTGGGTGCCCGGGATGAAGATGACATCAAACTGTCAGATGCAG
  ACCTCCGGGCTGAAGTGGACACATTCATGTTTGAAGGCCATGACACCACCACCAGTGGTATCTCCTGGTT
  TCTCTACTGCATGGCCCTGTACCCTGAGCACCAGCATCGTTGTAGAGAGGAGGTCCGCGAGATCCTAGGG
  GACCAGGACTTCTTCCAGTGGGATGATCTGGGCAAAATGACTTATCTGACCATGTGCATCAAGGAGAGCT
  TCCGCCTCTACCCACCTGTGCCCCAGGTGTACCGCCAGCTCAGCAAGCCTGTCACCTTTGTGGATGGCCG
  GTCTCTACCTGCAGGAAGCCTGATCTCTATGCATATCTATGCCCTCCATAGGAACAGTGCTGTATGGCCC
  GACCCTGAGGTCTTTGACTCTCTGCGCTTTTCCACTGAGAATGCATCCAAACGCCATCCCTTTGCCTTTA
  TGCCCTTCTCTGCTGGGCCCAGGAACTGCATTGGGCAGCAGTTTGCCATGAGTGAGATGAAGGTGGTCAC
  AGCCATGTGCTTGCTCCGCTTTGAGTTCTCTCTGGACCCCTCACGGCTGCCCATCAAGATGCCCCAGCTT
  GTCCTGCGCTCCAAGAATGGCTTTCACCTCCACCTGAAGCCACTGGGCCCTGGGTCTGGGAAGTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000779
• Insert Size: 2340 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000779.2, NP_000770.1
• RefSeq Size: 2170 bp
• RefSeq ORF: 1536 bp
• Locus ID: 1580
• Cytogenetics: 1p33
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. In rodents, the homologous protein has been shown to metabolize certain carcinogens; however, the specific function of the human protein has not been determined. Multiple transcript variants have been found for this gene. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is 1 aa shorter compared to isoform a.