CYP27A1 (NM_000784) Human Untagged Clone

CAT#: SC119685

CYP27A1 (untagged)-Human cytochrome P450, family 27, subfamily A, polypeptide 1 (CYP27A1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP27A1
• Synonyms: CP27; CTX; CYP27
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000784 edited
  ATCGGCACGAGGCGAGCACAACCCATGGCTGCGCTGGGCTGCGCGAGGCTGAGGTGGGCG
  CTGCGAGGGGCCGGCCGTGGCCTCTGCCCCCACGGGGCCAGAGCCAAGGCCGCGATCCCT
  GCCGCCCTCCCCTCGGACAAGGCCACCGGAGCTCCCGGAGCCGGGCCTGGTGTCCGGCGG
  CGGCAACGGAGCTTAGAGGAGATTCCACGTCTAGGACAGCTGCGCTTCTTCTTTCAGCTG
  TTCGTTCAAGGCTATGCCCTGCAACTGCACCAGTTACAGGTGCTTTACAAGGCCAAGTAC
  GGTCCAATGTGGATGTCCTACTTAGGGCCTCAGATGCACGTGAACCTGGCCAGTGCCCCG
  CTCTTGGAGCAAGTGATGCGGCAAGAGGGCAAGTACCCAGTACGGAACGACATGGAGCTA
  TGGAAGGAGCACCGGGACCAGCACGACCTGACCTATGGGCCGTTCACCACGGAAGGACAC
  CACTGGTACCAGCTGCGCCAGGCTCTGAACCAGCGGTTGCTGAAGCCAGCGGAAGCAGCG
  CTCTATACGGATGCTTTCAATGAGGTGATTGATGACTTTATGACTCGACTGGACCAGCTG
  CGGGCAGAGAGTGCTTCGGGGAACCAGGTGTCGGACATGGTTCAACTCTTCTACTACTTT
  GCCTTGGAAGCTATTTGCTACATCCTGTTCGAGAAACGCATTGGCTGCCTGCAGCGATCC
  ATCCCCGAGGACACCGTGACCTTCGTCAGATCCATCGGGTTAATGTTCCAGAACTCACTC
  TATGCCACCTTCCTCCCCAAGTGGACTCGCCCCGTGCTGCCTTTCTGGAAGCGATACCTG
  GATGGTTGGAATGCCATCTTTTCCTTTGGGAAGAAGCTGATTGATGAGAAGCTCGAAGAT
  ATGGAGGCCCAACTGCAGGCAGCAGGGCCAGATGGCATCCAGGTGTCTGGCTACCTGCAC
  TTCTTACTGGCCAGTGGACAGCTCAGTCCTCGGGAGGCCATGGGCAGCCTGCCTGAGCTG
  CTCATGGCTGGAGTGGACACGACATCCAACACGCTGACATGGGCCCTGTACCACCTCTCA
  AAGGACCCTGAGATCCAGGAGGCCTTGCACGAGGAAGTGGTGGGTGTGGTGCCAGCCGGG
  CAAGTGCCCCAGCACAAGGACTTTGCCCACATGCCGTTGCTCAAAGCTGTGCTTAAGGAG
  ACTCTGCGTCTCTACCCTGTGGTCCCCACAAACTCCCGGATCATAGAAAAGGAAATTGAA
  GTTGATGGCTTCCTCTTCCCCAAGAACACCCAGTTTGTGTTCTGCCACTATGTGGTGTCC
  CGGGACCCCACTGCCTTCTCTGAGCCTGAAAGCTTCCAGCCCCACCGCTGGCTGAGAAAC
  AGCCAGCCTGCTACCCCCAGGATCCAGCACCCATTTGGCTCTGTGCCCTTTGGCTATGGG
  GTCCGGGCCTGCCTGGGCCGCAGGATTGCAGAGCTGGAGATGCAGCTACTCCTCGCAAGG
  CTGATCCAGAAGTACAAGGTGGTCCTGGCCCCGGAGACGGGGGAGTTGAAGAGTGTGGCC
  CGCATTGTCCTGGTTCCCAATAAGAAAGTGGGCCTGCAGTTCCTGCAGAGACAGTGCTGA
  GCTGAGTCTCCGCCTTGCTGGGGCTTGTCCTAGAGGCTCCAGCTCTGGCACAGTGGTTCC
  TGGCTGCTGCCATGTCTCAGATGAGGAGGGAGAGAAGGAGGCCGCCAGACTCGAGAGGTG
  GGAGGAACTCCTTGCACACACCCTGAGCTTTTGCCACTTCTATCATTTTTGAGCAACTCC
  CTCTCAGCTAAAAGGCCACCCCTTTATCGCATTGCTGTCCTTGGGTAGAATATAAAATAA
  AGGGACTTTTATTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000784 unedited
  GCGGCAAAAAACATCGGCACGAGGCGAGCACAACTTATGGCTGCGCTGGGCTGCGCGAGG
  CTGAGGTGGGCGCTGCGAGGGGCCGGCCGTGGCCTCTGCCCCCACGGGGCCAGAGCCAAG
  GCCGCGATCCCTGCCGCCCTCCCCTCGGACAAGGCCACCGGAGCTCCCGGAGCCGGGCCT
  GGTGTCCTGCTGCGGCAACGGAGCTTAGAGGATATTCCACGTCTAGGACAGCTGCGCTTC
  TTCTTTCAGCTGTTCGTTCAAGGCTATGCCCTGCAACTGCACCAGTTACAGGTGCTTTAC
  AAGGCCAAGTACGGTCCAATGTGGATGTCCTACTTAGGGCCTCAGATGCACGTGAACCTG
  GCCAGTGCCCCGCTCTTGGAGCAAGTGATGCGGCAAGAGGGCAAGTACCCAGTACGGAAC
  GACATGGAGCTATGGAAGGAGCACCGGGACCAGCACGACCTGACCTATGGGCCGTTCACC
  ACGGAAGGACACCACTGGTACCAGCTGCGCCAGGCTCTGAACCAGCGGTTGCTGAAGCCA
  GCGGAAGCAGCGCTCTATACGGATGCTTTCAATGAGGTGATTGATGACTTTATGACTCGA
  CTGGACCAGCTGCGGGCAGAGAGTGCTTCGGGGAACCAGGTGTCGGACATGGTTCAACTC
  TTCTACTACTTTGCCTTGGAAGCTATTTGCTACATCCTGTTCGAGAAACGCATTGGCTGC
  CTGCAGCGATCCATCCCCGAGGACACCGTGACCTTCGTCAGATCCATCGGGTTAATGTTC
  CAGAACTCACTCTATGCCACCTTCCTCCCCCAGTGGACTCGCCCCGTGCTGGCCTTTCTG
  GAAGCGATACCTTGGATGGTTGGATGCCAATCTTTTCCTTTGGAAGAAGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000784 unedited
  TTTAGAGTCCTTTTTTTATTTCTACCCAGGGACAGCAATGCGATAAAGGGTGGCCTTTTA
  GCTGAGAGGGAGTTGCTCAAAAAGATAGAAGTGGCAAAAGCTCAGGGTGTGTGCAAGGAG
  TTCCTCCCACCTCTCGAGTCTGGCGGCCTCCTTCTCTCCCTCCTCATCTGAGACATGGCA
  GCAGCCAGGAACCACTGTGCCAGAGCTGGAGCCTCTAGGACAAGCCCCAGCAAGGCGGAG
  ACTCAGCTCAGCACTGTCTCTGCAGGAACTGCAGGCCCACTTTCTTATTGGGAACCAGGA
  CAATGCGGGCCACACTCTTCAACTCCCCCGTCTCCGGGGCCAGGACCACCTTGTACTTCT
  GGATCAGCCTTGCGAGGAGTAGCTGCATCTCCAGCTCTGCAATCCTGCGGCCCAGGCAGG
  CCCGGACCCCATAGCCAAAGGGCACAGAGCCAAATGGGTGCTGGATCCTGGGGGTAGCAG
  GCTGGCTGTTTCTCAGCCAGCGGTGGGGCTGGAAGCTTTCAGGCTCAGAGAAGGCAGTGG
  GGTCCCGGGACACCACATAGTGGCAGAACACAAACTGGGTGTTCTTGGGGAAGAGGAAGC
  CATCAACTTCAATTTCCTTTTCTATGATCCCGGCAGTTTGTGGGGACCACCAGGGTAGAG
  ACGCAGAGTCTCCCTTTAGCACAGNCTCTGAGCAACGGCATGGGGGCCAAGTCCCTTGGG
  CTGGGGGCACTTGCCCCGGCTGGCACCCCACCCACCACTTCCTCGTGCAAGGGCCTCCTG
  GATCTCAAGGGTCCTCTGAGAGGTGGGACAGGGCCCCATGTCACCCTGGTGGAGGTCGTG
  TCCCACTCCACCATGACCACTTCAGGCGGCCTGGCCATGGCCTCCCAGGACTGAACTGCC
  CACTGCCCCTAGAAGTGCAGGGAGCAAACACCTGGATGCCACTGCCCCTCTTGCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000784
• Insert Size: 2060 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000784.2, NP_000775.1
• RefSeq Size: 2059 bp
• RefSeq ORF: 1596 bp
• Locus ID: 1593
• Cytogenetics: 2q35
• Domains: p450
• Protein Families: Druggable Genome, P450
• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Primary bile acid biosynthesis
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq, Jul 2008]