Angiotensin Converting Enzyme 1 (ACE) (NM_000789) Human Untagged Clone

CAT#: SC119689

ACE (untagged)-Human angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 (ACE), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACE
• Synonyms: ACE1; CD143; DCP; DCP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000789, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGGCCGCCTCGGGCCGCCGGGGGCCGGGGCTGCTGCTGCCGCTGCCGCTGCTGTTGCTGCTGCCGC
  CGCAGCCCGCCCTGGCGTTGGACCCCGGGCTGCAGCCCGGCAACTTTTCTGCTGACGAGGCCGGGGCGCA
  GCTCTTCGCGCAGAGCTACAACTCCAGCGCCGAACAGGTGCTGTTCCAGAGCGTGGCCGCCAGCTGGGCG
  CACGACACCAACATCACCGCGGAGAATGCAAGGCGCCAGGAGGAAGCAGCCCTGCTCAGCCAGGAGTTTG
  CGGAGGCCTGGGGCCAGAAGGCCAAGGAGCTGTATGAACCGATCTGGCAGAACTTCACGGACCCGCAGCT
  GCGCAGGATCATCGGAGCTGTGCGCACCCTGGGCTCTGCCAACCTGCCCCTGGCTAAGCGGCAGCAGTAC
  AACGCCCTGCTAAGCAACATGAGCAGGATCTACTCCACCGCCAAGGTCTGCCTCCCCAACAAGACTGCCA
  CCTGCTGGTCCCTGGACCCAGATCTCACCAACATCCTGGCTTCCTCGCGAAGCTACGCCATGCTCCTGTT
  TGCCTGGGAGGGCTGGCACAACGCTGCGGGCATCCCGCTGAAACCGCTGTACGAGGATTTCACTGCCCTC
  AGCAATGAAGCCTACAAGCAGGACGGCTTCACAGACACGGGGGCCTACTGGCGCTCCTGGTACAACTCCC
  CCACCTTCGAGGACGATCTGGAACACCTCTACCAACAGCTAGAGCCCCTCTACCTGAACCTCCATGCCTT
  CGTCCGCCGCGCACTGCATCGCCGATACGGAGACAGATACATCAACCTCAGGGGACCCATCCCTGCTCAT
  CTGCTGGGAGACATGTGGGCCCAGAGCTGGGAAAACATCTACGACATGGTGGTGCCTTTCCCAGACAAGC
  CCAACCTCGATGTCACCAGTACTATGCTGCAGCAGGGCTGGAACGCCACGCACATGTTCCGGGTGGCAGA
  GGAGTTCTTCACCTCCCTGGAGCTCTCCCCCATGCCTCCCGAGTTCTGGGAAGGGTCGATGCTGGAGAAG
  CCGGCCGACGGGCGGGAAGTGGTGTGCCACGCCTCGGCTTGGGACTTCTACAACAGGAAAGACTTCAGGA
  TCAAGCAGTGCACACGGGTCACGATGGACCAGCTCTCCACAGTGCACCATGAGATGGGCCATATACAGTA
  CTACCTGCAGTACAAGGATCTGCCCGTCTCCCTGCGTCGGGGGGCCAACCCCGGCTTCCATGAGGCCATT
  GGGGACGTGCTGGCGCTCTCGGTCTCCACTCCTGAACATCTGCACAAAATCGGCCTGCTGGACCGTGTCA
  CCAATGACACGGAAAGTGACATCAATTACTTGCTAAAAATGGCACTGGAAAAAATTGCCTTCCTGCCCTT
  TGGCTACTTGGTGGACCAGTGGCGCTGGGGGGTCTTTAGTGGGCGTACCCCCCCTTCCCGCTACAACTTC
  GACTGGTGGTATCTTCGAACCAAGTATCAGGGGATCTGTCCTCCTGTTACCCGAAACGAAACCCACTTTG
  ATGCTGGAGCTAAGTTTCATGTTCCAAATGTGACACCATACATCAGGTACTTTGTGAGTTTTGTCCTGCA
  GTTCCAGTTCCATGAAGCCCTGTGCAAGGAGGCAGGCTATGAGGGCCCACTGCACCAGTGTGACATCTAC
  CGGTCCACCAAGGCAGGGGCCAAGCTCCGGAAGGTGCTGCAGGCTGGCTCCTCCAGGCCCTGGCAGGAGG
  TGCTGAAGGACATGGTCGGCTTAGATGCCCTGGATGCCCAGCCGCTGCTCAAGTACTTCCAGCCAGTCAC
  CCAGTGGCTGCAGGAGCAGAACCAGCAGAACGGCGAGGTCCTGGGCTGGCCCGAGTACCAGTGGCACCCG
  CCGTTGCCTGACAACTACCCGGAGGGCATAGACCTGGTGACTGATGAGGCTGAGGCCAGCAAGTTTGTGG
  AGGAATATGACCGGACATCCCAGGTGGTGTGGAACGAGTATGCCGAGGCCAACTGGAACTACAACACCAA
  CATCACCACAGAGACCAGCAAGATTCTGCTGCAGAAGAACATGCAAATAGCCAACCACACCCTGAAGTAC
  GGCACCCAGGCCAGGAAGTTTGATGTGAACCAGTTGCAGAACACCACTATCAAGCGGATCATAAAGAAGG
  TTCAGGACCTAGAACGGGCAGCACTGCCTGCCCAGGAGCTGGAGGAGTACAACAAGATCCTGTTGGATAT
  GGAAACCACCTACAGCGTGGCCACTGTGTGCCACCCGAATGGCAGCTGCCTGCAGCTCGAGCCAGATCTG
  ACGAATGTGATGGCCACGTCCCGGAAATATGAAGACCTGTTATGGGCATGGGAGGGCTGGCGAGACAAGG
  CGGGGAGAGCCATCCTCCAGTTTTACCCGAAATACGTGGAACTCATCAACCAGGCTGCCCGGCTCAATGG
  CTATGTAGATGCAGGGGACTCGTGGAGGTCTATGTACGAGACACCATCCCTGGAGCAAGACCTGGAGCGG
  CTCTTCCAGGAGCTGCAGCCACTCTACCTCAACCTGCATGCCTACGTGCGCCGGGCCCTGCACCGTCACT
  ACGGGGCCCAGCACATCAACCTGGAGGGGCCCATTCCTGCTCACCTGCTGGGGAACATGTGGGCGCAGAC
  CTGGTCCAACATCTATGACTTGGTGGTGCCCTTCCCTTCAGCCCCCTCGATGGACACCACAGAGGCTATG
  CTAAAGCAGGGCTGGACGCCCAGGAGGATGTTTAAGGAGGCTGATGATTTCTTCACCTCCCTGGGGCTGC
  TGCCCGTGCCTCCTGAGTTCTGGAACAAGTCGATGCTGGAGAAGCCAACCGACGGGCGGGAGGTGGTCTG
  CCACGCCTCGGCCTGGGACTTCTACAACGGCAAGGACTTCCGGATCAAGCAGTGCACCACCGTGAACTTG
  GAGGACCTGGTGGTGGCCCACCACGAAATGGGCCACATCCAGTATTTCATGCAGTACAAAGACTTACCTG
  TGGCCTTGAGGGAGGGTGCCAACCCCGGCTTCCATGAGGCCATTGGGGACGTGCTAGCCCTCTCAGTGTC
  TACGCCCAAGCACCTGCACAGTCTCAACCTGCTGAGCAGTGAGGGTGGCAGCGACGAGCATGACATCAAC
  TTTCTGATGAAGATGGCCCTTGACAAGATCGCCTTTATCCCCTTCAGCTACCTCGTCGATCAGTGGCGCT
  GGAGGGTATTTGATGGAAGCATCACCAAGGAGAACTATAACCAGGAGTGGTGGAGCCTCAGGCTGAAGTA
  CCAGGGCCTCTGCCCCCCAGTGCCCAGGACTCAAGGTGACTTTGACCCAGGGGCCAAGTTCCACATTCCT
  TCTAGCGTGCCTTACATCAGGTACTTTGTCAGCTTCATCATCCAGTTCCAGTTCCACGAGGCACTGTGCC
  AGGCAGCTGGCCACACGGGCCCCCTGCACAAGTGTGACATCTACCAGTCCAAGGAGGCCGGGCAGCGCCT
  GGCGACCGCCATGAAGCTGGGCTTCAGTAGGCCGTGGCCGGAAGCCATGCAGCTGATCACGGGCCAGCCC
  AACATGAGCGCCTCGGCCATGTTGAGCTACTTCAAGCCGCTGCTGGACTGGCTCCGCACGGAGAACGAGC
  TGCATGGGGAGAAGCTGGGCTGGCCGCAGTACAACTGGACGCCGAACTCCGCTCGCTCAGAAGGGCCCCT
  CCCAGACAGCGGCCGCGTCAGCTTCCTGGGCCTGGACCTGGATGCGCAGCAGGCCCGCGTGGGCCAGTGG
  CTGCTGCTCTTCCTGGGCATCGCCCTGCTGGTAGCCACCCTGGGCCTCAGCCAGCGGCTCTTCAGCATCC
  GCCACCGCAGCCTCCACCGGCACTCCCACGGGCCCCAGTTCGGCTCCGAGGTGGAGCTGAGACACTCCTG
  A
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000789 unedited
  GCACGAGGCCGCGCACCGCGTCATGGGGGCCGCCTCGGGCCGCCGGGGGCCGGGGCTGCT
  GCTGCCGCTGCCGCTGCTGTTGCTGCTGCCGCCGCAGCCCGCCCTGGCGTTGGACCCCGG
  GCTGCAGCCCGGCAACTTTTCTGCTGACGAGGCCGGGGCGCAGCTCTTCGCGCAGAGCTA
  CAACTCCAGCGCCGAACAGGTGCTGTTCCAGAGCGTGGCCGCCAGCTGGGCGCACGACAC
  CAACATCACCGCGGAGAATGCAAGGCGCCAGGAGGAAGCAGCCCTGCTCAGCCAGGAGTT
  TGCGGAGGCCTGGGGCCAGAAGGCCAAGGAGCTGTATGAACCGATCTGGCAGAACTTCAC
  GGACCCGCAGCTGCGCAGGATCATCGGAGCTGTGCGCACCCTGGGCTCTGCCAACCTGCC
  CCTGGCTAAGCGGCAGCAGTACAACGCCCTGCTAAGCAACATGAGCAGGATCTACTCCAC
  CGCCAAGGTCTGCCTCCCCAACAAGACTGCCACCTGCTGGTCCCTGGACCCAGATCTCAC
  CAACATCCTGGCTTCCTCGCGAAGCTACGCCATGCTCCTGTTTGCCTGGGAGGGCTGGCA
  CAATGCTGCGGGCATCCCGCTGAAACCGCTGTACGAGGATTTCACTGCCCTCAGCATGAA
  GCCTACAGCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000789
• Insert Size: 5300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000789.2, NP_000780.1
• RefSeq Size: 4969 bp
• RefSeq ORF: 3921 bp
• Locus ID: 1636
• Cytogenetics: 17q23.3
• Domains: Peptidase_M2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease, Secreted Protein, Transmembrane
• Protein Pathways: Hypertrophic cardiomyopathy (HCM), Renin-angiotensin system
• Gene Summary: 'This gene encodes an enzyme involved in blood pressure regulation and electrolyte balance. It catalyzes the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This angiotensin converting enzyme (ACE) also inactivates the vasodilator protein, bradykinin. Accordingly, the encoded enzyme increases blood pressure and is a drug target of ACE inhibitors, which are often prescribed to reduce blood pressure. This enzyme additionally plays a role in fertility through its ability to cleave and release GPI-anchored membrane proteins in spermatozoa. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme. This polymorphism, as well as mutations in this gene, have been implicated in a wide variety of diseases including cardiovascular pathophysiologies, psoriasis, renal disease, stroke, and Alzheimer's disease. Regulation of the homologous ACE2 gene may be involved in progression of disease caused by several human coronaviruses, including SARS-CoV and SARS-CoV-2. Alternative splicing results in multiple transcript variants encoding both somatic (sACE) and male-specific testicular (tACE) isoforms. [provided by RefSeq, Sep 2020]'
  Transcript Variant: This variant (1), also known as the endothelial or somatic form, represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.