Sodium Potassium ATPase (ATP1A1) (NM_000701) Human Untagged Clone

CAT#: SC119714

ATP1A1 (untagged)-Human ATPase, Na+/K+ transporting, alpha 1 polypeptide (ATP1A1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP1A1
• Synonyms: CMT2DD; HOMGSMR2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000701, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGAAGGGGGTTGGACGTGATAAGTATGAGCCTGCAGCTGTTTCAGAACAAGGTGATAAAAAGGGCA
  AAAAGGGCAAAAAAGACAGGGACATGGATGAACTGAAGAAAGAAGTTTCTATGGATGATCATAAACTTAG
  CCTTGATGAACTTCATCGTAAATATGGAACAGACTTGAGCCGGGGATTAACATCTGCTCGTGCAGCTGAG
  ATCCTGGCGCGAGATGGTCCCAACGCCCTCACTCCCCCTCCCACTACTCCTGAATGGATCAAGTTTTGTC
  GGCAGCTCTTTGGGGGGTTCTCAATGTTACTGTGGATTGGAGCGATTCTTTGTTTCTTGGCTTATAGCAT
  CCAAGCTGCTACAGAAGAGGAACCTCAAAACGATAATCTGTACCTGGGTGTGGTGCTATCAGCCGTTGTA
  ATCATAACTGGTTGCTTCTCCTACTATCAAGAAGCTAAAAGTTCAAAGATCATGGAATCCTTCAAAAACA
  TGGTCCCTCAGCAAGCCCTTGTGATTCGAAATGGTGAGAAAATGAGCATAAATGCGGAGGAAGTTGTGGT
  TGGGGATCTGGTGGAAGTAAAAGGAGGAGACCGAATTCCTGCTGACCTCAGAATCATATCTGCAAATGGC
  TGCAAGGTGGATAACTCCTCGCTCACTGGTGAATCAGAACCCCAGACTAGGTCTCCAGATTTCACAAATG
  AAAACCCCCTGGAGACGAGGAACATTGCCTTCTTTTCAACCAATTGTGTTGAAGGCACCGCACGTGGTAT
  TGTTGTCTACACTGGGGATCGCACTGTGATGGGAAGAATTGCCACACTTGCTTCTGGGCTGGAAGGAGGC
  CAGACCCCCATTGCTGCAGAAATTGAACATTTTATCCACATCATCACGGGTGTGGCTGTGTTCCTGGGTG
  TGTCTTTCTTCATCCTTTCTCTCATCCTTGAGTACACCTGGCTTGAGGCTGTCATCTTCCTCATCGGTAT
  CATCGTAGCCAATGTGCCGGAAGGTTTGCTGGCCACTGTCACGGTCTGTCTGACACTTACTGCCAAACGC
  ATGGCAAGGAAAAACTGCTTAGTGAAGAACTTAGAAGCTGTGGAGACCTTGGGGTCCACGTCCACCATCT
  GCTCTGATAAAACTGGAACTCTGACTCAGAACCGGATGACAGTGGCCCACATGTGGTTTGACAATCAAAT
  CCATGAAGCTGATACGACAGAGAATCAGAGTGGTGTCTCTTTTGACAAGACTTCAGCTACCTGGCTTGCT
  CTGTCCAGAATTGCAGGTCTTTGTAACAGGGCAGTGTTTCAGGCTAACCAGGAAAACCTACCTATTCTTA
  AGCGGGCAGTTGCAGGAGATGCCTCTGAGTCAGCACTCTTAAAGTGCATAGAGCTGTGCTGTGGTTCCGT
  GAAGGAGATGAGAGAAAGATACGCCAAAATCGTCGAGATACCCTTCAACTCCACCAACAAGTACCAGTTG
  TCTATTCATAAGAACCCCAACACATCGGAGCCCCAACACCTGTTGGTGATGAAGGGCGCCCCAGAAAGGA
  TCCTAGACCGTTGCAGCTCTATCCTCCTCCACGGCAAGGAGCAGCCCCTGGATGAGGAGCTGAAAGACGC
  CTTTCAGAACGCCTATTTGGAGCTGGGGGGCCTCGGAGAACGAGTCCTAGGTTTCTGCCACCTCTTTCTG
  CCAGATGAACAGTTTCCTGAAGGGTTCCAGTTTGACACTGACGATGTGAATTTCCCTATCGATAATCTGT
  GCTTTGTTGGGCTCATCTCCATGATTGACCCTCCACGGGCGGCCGTTCCTGATGCCGTGGGCAAATGTCG
  AAGTGCTGGAATTAAGGTCATCATGGTCACAGGAGACCATCCAATCACAGCTAAAGCTATTGCCAAAGGT
  GTGGGCATCATCTCAGAAGGCAATGAGACCGTGGAAGACATTGCTGCCCGCCTCAACATCCCAGTCAGCC
  AGGTGAACCCCAGGGATGCCAAGGCCTGCGTAGTACACGGCAGTGATCTAAAGGACATGACCTCCGAGCA
  GCTGGATGACATTTTGAAGTACCACACTGAGATAGTGTTTGCCAGGACCTCCCCTCAGCAGAAGCTCATC
  ATTGTGGAAGGCTGCCAAAGACAGGGTGCTATCGTGGCTGTGACTGGTGACGGTGTGAATGACTCTCCAG
  CTTTGAAGAAAGCAGACATTGGGGTTGCTATGGGGATTGCTGGCTCAGATGTGTCCAAGCAAGCTGCTGA
  CATGATTCTTCTGGATGACAACTTTGCCTCAATTGTGACTGGAGTAGAGGAAGGTCGTCTGATCTTTGAT
  AACTTGAAGAAATCCATTGCTTATACCTTAACCAGTAACATTCCCGAGATCACCCCGTTCCTGATATTTA
  TTATTGCAAACATTCCACTACCACTGGGGACTGTCACCATCCTCTGCATTGACTTGGGCACTGACATGGT
  TCCTGCCATCTCCCTGGCTTATGAGCAGGCTGAGAGTGACATCATGAAGAGACAGCCCAGAAATCCCAAA
  ACAGACAAACTTGTGAATGAGCGGCTGATCAGCATGGCCTATGGGCAGATTGGAATGATCCAGGCCCTGG
  GAGGCTTCTTTACTTACTTTGTGATTCTGGCTGAGAACGGCTTCCTCCCAATTCACCTGTTGGGCCTCCG
  AGTGGACTGGGATGACCGCTGGATCAACGATGTGGAAGACAGCTACGGGCAGCAGTGGACCTATGAGCAG
  AGGAAAATCGTGGAGTTCACCTGCCACACAGCCTTCTTCGTCAGTATCGTGGTGGTGCAGTGGGCCGACT
  TGGTCATCTGTAAGACCAGGAGGAATTCGGTCTTCCAGCAGGGGATGAAGAACAAGATCTTGATATTTGG
  CCTCTTTGAAGAGACAGCCCTGGCTGCTTTCCTTTCCTACTGCCCTGGAATGGGTGTTGCTCTTAGGATG
  TATCCCCTCAAACCTACCTGGTGGTTCTGTGCCTTCCCCTACTCTCTTCTCATCTTCGTATATGACGAAG
  TCAGAAAACTCATCATCAGGCGACGCCCTGGCGGCTGGGTGGAGAAGGAAACCTACTATTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000701 unedited
  GCACATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGCTCCCTCCCTT
  GGCTCCCCTGGTCCCGCTCGCTCGGCCGGGAGCTGCTCTGTGCTTTTCTCTCTGATTCTC
  CAGCGACAGGACCCGGCGCCGGGCACTGAGCACCGCCACCATGGGGAAGGGGGTTGGACG
  TGATAAGTATGAGCCTGCAGCTGTTTCAGAACAAGGTGATAAAAAGGGCAAAAAGGGCAA
  AAAAGACAGGGACATGGATGAACTGAAGAAAGAAGTTTCTATGGATGATCATAAACTTAG
  CCTTGATGAACTTCATCGTAAATATGGAACAGACTTGAGCCGGGGATTAACATCTGCTCG
  TGCAGCTGAGATCCTGGCGCGAGATGGTCCCAACGCCCTCACTCCCCCTCCCACTACTCC
  TGAATGGATCAAGTTTTGTCGGCAGCTCTTTGGGGGGTTCTCAATGTTACTGTGGATTGG
  AGCGATTCTTTGTTTCTTGGCTTATAGCATCCAAGCTGCTACAGAAGAGGAACCTCAAAA
  CGATAATCTGTACCTGGGTGTGGTGCTATCAGCCGTTGTAATCATAACTGGTTGCTTCTC
  CTACTATCAAGAAGCTAAAAGTTCAAAGATCATGGAATCCTTCAAAAACATGGTCCCTCA
  GCAAGCCCTTGTGATTCGAAATGGTGAGAAAATGAGCATAAATGCGGAGGAAGTTGTGGT
  TGGGGATCTGGTGGAAGTAAAAGGGAGGAGACCGATTCCTGCTGACCTCAGAATCATATC
  TGCANATGGCTGCAAGGTGGATAACTCCTCGCTCACTGGTGAATCAGAACCCCAGACTAN
  GTCTNNCAGATTCACAAATGANAACCCCCTGGAGACGAGAACATTGNCTTCTTTTCANCN
  ANTGTGTTGAAGGCACGNACGTGGTATGTTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000701 unedited
  GAACGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCCATTATAATAGCCAT
  CTTTATTTGTAAAAATCCAGATATAAAATGTATTCTTTCAGTCTTTCCGGGTGTTCCTTT
  TTTACAAAAACAAAAAGGCACATAAAAACCTTCCCCGCTGTCATTTCCACAGATGGATGG
  TTTTCAGGCAGCCCTCCCCCCTCCCCCCATAGAGCTACATGCTTCATTCCAGGACGTCTT
  GCTTCCCCACATGCTGCGGTGCTTTCCTACCAGGGTAGAGTTCCAAACTCCAAGACTGAA
  GTACACAAAGAGGGGGTGGGTGTCGGATGCAAAGTGTGTGGCCTGATGCTCCACGGCGTG
  CAGGACGGGGGGCTAATAGTAGGTTTCCTTCTCCACCCAGCCGCCAGGGCGTCGCCTGAT
  GATGAGTTTTCTGACTTCGTCATATACGAAGATGACAAGAGAGTAGGGGAAGGCACAGAA
  CCACCAGGTAGGTTTGAGGGGATACATCCTAAGAGCAACACCCATTCCAGGGCAGTAGGA
  AAGGAAAGCAGCCAGGGCTGTCTTTTTCAAAGAGGCCAAATATCAAGATCTTGTTCTTCA
  TCCCCTGCCTGAAAGACCGAATTCCTCCTGGTCTAACAGATGACCAAGTCGGCCCACTGC
  ACCACCACGATACTGACGAAGAAAGCTGTGTGGCAGGGGAACTCCAGATTTTCCTTTGTT
  ATAAGCCCCCTGCTGCCCGAACTTGTTTTCCAATCGTTATCCAGCGGCCATCCAGTCCCT
  CCGGAGCCCCACAGGGGAATGGGAAGAAACCGTTTTCAACCCCAAACACAAATAATTAAA
  AAATCCTCCCAGGCCTGGATCTATCTATTTCCCTTAGGCCTGCTGAACAACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000701
• Insert Size: 4040 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000701.6, NP_000692.2
• RefSeq Size: 3713 bp
• RefSeq ORF: 3072 bp
• Locus ID: 476
• Cytogenetics: 1p13.1
• Domains: E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cardiac muscle contraction
• Gene Summary: 'The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]'
  Transcript Variant: This variant (1) represents use of an alternate promoter and 5' exon, compared to variant 3. The resulting isoform (a) is the same length but has a distinct N-terminus, compared to isoform c.