ATP1A2 (NM_000702) Human Untagged Clone

CAT#: SC119715

ATP1A2 (untagged)-Human ATPase, Na+/K+ transporting, alpha 2 polypeptide (ATP1A2)


  "NM_000702" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ATP1A2
Synonyms FHM2; MHP2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119715 sequence for NM_000702 edited (data generated by NextGen Sequencing)
ATGGGCCGTGGGGCTGGCCGTGAGTACTCACCTGCCGCCACCACGGCAGAGAATGGGGGC
GGCAAGAAGAAACAGAAGGAGAAGGAACTGGATGAGCTGAAGAAGGAGGTGGCAATGGAT
GACCACAAGCTGTCCTTGGATGAGCTGGGCCGCAAATACCAAGTGGACCTGTCCAAGGGC
CTCACCAACCAGCGGGCTCAGGACGTTCTGGCTCGAGATGGGCCCAACGCCCTCACACCA
CCTCCCACAACCCCTGAGTGGGTCAAGTTCTGCCGTCAGCTTTTCGGGGGGTTCTCCATC
CTGCTGTGGATTGGGGCTATCCTCTGCTTCCTGGCCTACGGCATCCAGGCTGCCATGGAG
GATGAACCATCCAACGACAATCTATATCTGGGTGTGGTGCTGGCAGCTGTGGTCATTGTC
ACTGGCTGCTTCTCCTACTACCAGGAGGCCAAGAGCTCCAAGATCATGGATTCCTTCAAG
AACATGGTACCTCAGCAAGCCCTTGTGATCCGGGAGGGAGAGAAGATGCAGATCAACGCA
GAGGAAGTGGTGGTGGGAGACCTGGTGGAGGTGAAGGGTGGAGACCGCGTCCCTGCTGAC
CTCCGGATCATCTCTTCTCATGGCTGTAAGGTGGATAACTCATCCTTAACAGGAGAGTCG
GAGCCCCAGACCCGCTCCCCCGAGTTCACCCATGAGAACCCCCTGGAGACCCGCAATATC
TGTTTCTTCTCCACCAACTGTGTTGAAGGCACTGCCAGGGGCATTGTGATTGCCACAGGA
GACCGGACGGTGATGGGCCGCATAGCTACTCTCGCCTCAGGCCTGGAGGTTGGGCGGACA
CCCATAGCAATGGAGATTGAACACTTCATCCAGCTGATCACAGGGGTCGCTGTATTCCTG
GGGGTCTCCTTCTTCGTGCTCTCCCTCATCCTGGGCTACAGCTGGCTGGAGGCAGTCATC
TTCCTCATCGGCATCATAGTGGCCAACGTGCCTGAGGGGCTTCTGGCCACTGTCACTGTG
TGCCTGACCCTGACAGCCAAGCGCATGGCACGGAAGAACTGCCTGGTGAAGAACCTGGAG
GCGGTGGAGACGCTGGGCTCCACGTCCACCATCTGCTCGGACAAGACGGGCACCCTCACC
CAGAACCGCATGACCGTCGCCCACATGTGGTTCGACAACCAAATCCATGAGGCTGACACC
ACCGAAGATCAGTCTGGGGCCACTTTTGACAAACGATCCCCTACGTGGACGGCCCTGTCT
CGAATTGCTGGTCTCTGCAACCGCGCCGTCTTCAAGGCAGGACAGGAGAACATCTCCGTG
TCTAAGCGGGACACAGCTGGTGATGCCTCTGAGTCAGCTCTGCTCAAGTGCATTGAGCTC
TCCTGTGGCTCAGTGAGGAAAATGAGAGACAGAAACCCCAAGGTGGCAGAGATTCCTTTC
AACTCTACCAACAAGTACCAGCTGTCTATCCACGAGCGAGAAGACAGCCCCCAGAGCCAC
GTGCTGGTGATGAAGGGGGCCCCAGAGCGCATTCTGGACCGGTGCTCCACCATCCTGGTG
CAGGGCAAGGAGATCCCGCTCGACAAGGAGATGCAAGATGCCTTTCAAAATGCCTACATG
GAGCTGGGGGGACTTGGGGAGCGTGTGCTGGGATTCTGTCAACTGAATCTGCCATCTGGA
AAGTTTCCTCGGGGCTTCAAATTCGACACGGATGAGCTGAACTTTCCCACGGAGAAGCTT
TGCTTTGTGGGGCTCATGTCTATGATTGACCCTCCCCGGGCTGCTGTGCCAGATGCTGTG
GGCAAGTGCCGAAGCGCAGGCATCAAGGTGATCATGGTAACCGGGGATCACCCTATCACA
GCCAAGGCCATTGCCAAAGGCGTGGGCATCATATCAGAGGGTAACGAGACTGTGGAGGAC
ATTGCAGCCCGGCTCAACATTCCCATGAGTCAAGTCAACCCCAGAGAAGCCAAGGCATGC
GTGGTGCACGGCTCTGACCTGAAGGACATGACATCGGAGCAGCTCGATGAGATCCTCAAG
AACCACACAGAGATCGTCTTTGCTCGAACGTCTCCCCAGCAGAAGCTCATCATTGTGGAG
GGATGTCAGAGGCAGGGAGCCATTGTGGCCGTGACGGGTGACGGGGTGAACGACTCCCCT
GCATTGAAGAAGGCTGACATTGGCATTGCCATGGGCATCTCTGGCTCTGACGTCTCTAAG
CAGGCAGCCGACATGATCCTGCTGGATGACAACTTTGCCTCCATCGTCACGGGGGTGGAG
GAGGGCCGCCTGATCTTTGACAACTTGAAGAAATCCATCGCCTACACCCTGACCAGCAAC
ATCCCCGAGATCACCCCCTTCCTGCTGTTCATCATTGCCAACATCCCCCTACCTCTGGGC
ACTGTGACCATCCTTTGCATTGACCTGGGCACAGATATGGTCCCTGCCATCTCCTTGGCC
TATGAGGCAGCTGAGAGTGATATCATGAAGCGGCAGCCACGAAACTCCCAGACGGACAAG
CTGGTGAATGAGAGGCTCATCAGCATGGCCTACGGACAGATCGGGATGATCCAGGCACTG
GGTGGCTTCTTCACCTACTTTGTGATCCTGGCAGAGAACGGTTTCCTGCCATCACGGCTA
CTGGGAATCCGCCTCGACTGGGATGACCGGACCATGAATGATCTGGAGGACAGCTATGGA
CAGGAGTGGACCTATGAGCAGCGGAAGGTGGTGGAGTTCACGTGCCACACGGCATTCTTT
GCCAGCATCGTGGTGGTGCAGTGGGCTGACCTCATCATCTGCAAGACCCGCCGCAACTCA
GTCTTCCAGCAGGGCATGAAGAACAAGATCCTGATTTTTGGGCTCCTGGAGGAGACGGCG
TTGGCTGCCTTTCTCTCTTACTGCCCAGGCATGGGTGTAGCCCTCCGCATGTACCCGCTC
AAAGTCACCTGGTGGTTCTGCGCCTTCCCCTACAGCCTCCTCATCTTCATCTATGATGAG
GTCCGAAAGCTCATCCTGCGGCGGTATCCTGGTGGCTGGGTGGAGAAGGAGACATACTAC
TGA

Clone variation with respect to NM_000702.3
>OriGene 5' read for NM_000702 unedited
GGCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCCGAGGTCTCCGACTGTC
CCAGACGGGCTGGTGTGGGCTTGGGATCCTCCTGGTGACCTCTCCCGCTAAGGTCCCTCA
GCCACTCTGCCCCAAGATGGGCCGTGGGGCTGGCCGTGAGTACTCACCTGCCGCCACCAC
GGCAGAGAATGGGGGCGGCAAGAAGAAACAGAAGGAGAAGGAACTGGATGAGCTGAAGAA
GGAGGTGGCAATGGATGACCACAAGCTGTCCTTGGATGAGCTGGGCCGCAAATACCAAGT
GGACCTGTCCAAGGGCCTCACCAACCAGCGGGCTCAGGACGTTCTGGCTCGAGATGGGCC
CAACGCCCTCACACCACCTCCCACAACCCCTGAGTGGGTCAAGTTCTGCCGTCAGCTTTT
CGGGGGGTTCTCCATCCTGCTGTGGATTGGGGCTATCCTCTGCTTCCTGGCCTACGGCAT
CCAGGCTGCCATGGAGGATGAACCATCCAACGACAATCTATATCTGGGTGTGGTGCTGGC
AGCTGTGGTCATTGTCACTGGCTGCTTCTCCTACTACCAGGAGGCCAAGAGCTCCAAGAT
CATGGATTCCTTCAAGAACATGGTACCTCAGCAAGCCCTTGTGATCCGGGAGGGAGAGAA
GATGCAGATCAACGCATAGGAAGTGGTGGTGGGAGAACTGGTGGAGGTGAAGGGTGGAGA
CCGCGTCCCTGCTGACCCCCGGATCACCTCTTTTATGGCTGTAAGGTGGAAACTCATCCT
TACCAGAGAGTTCGACCCCCAGACCCGTCCCCCGAGTTACCCATGAGAACCCCTGGAGAC
CCGGCATATCTGGTTTCTTCTCCCCCACTGTGTTGAAAGCCCTGCCCAGGCCTTTGGGAT
TGC
>OriGene 3' read for NM_000702 unedited
CCGCGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTGAGAGGAGTCTCACCCTT
TCGCCCAGGCTGGAGTGTAATGGCACGATCTCAGCTCACTGCAACCTCTTCCTCACAGGT
TCAAGCAATTCTCCTGCCTCAGCCTCCTGAGTAGCTGAGATTACAGGCGTGTGCCACCAT
GACTGGCTAATTTTTTGTACTTTTAGTAGAGACAGGGTTTCACCATGTTGGCCAGGCTCG
CCTCGAACTCCTGACCTCATGATCCACCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAG
GCATGAGCCACCGCACCCAGAAAAAGCAAATCTCTTAGTATTTTTCCTCTTGTCCAAAGG
TTCTGACCATGTTCATGACCTAAGCTTGTCCCTGGAAGCATACATGTCCCTGGGAGACAG
GAAGGTTCAGGGAACTTCTCCACCCTGATTCTAGCCAATGGCCAGCGAACTCCTTTTCCA
GGGTTGGCTTGGCAGTGTCGGGGAATGATGCAGGTTCATCGGGATGGGCATGAAAAGAAA
GGGAATGGAAGAGGCTGGGAAAGAACTTGTGGCCATCCCTTTCAGAACAGCATGGAGCTC
TGANGACTTAGTATTACAGAAGGTGAAGTGTTATTAAGTACATGAGTGACATCCGGACCT
TGTGGACTAAGAAGGACCCTACCAAAGGTCAGCGGGGAACTCTGTGCACACAAGGTGGCA
TTTCTGGAGCCTCTGATTGCCTCTGGCCTGCTCTGCTGCACCCTGAGAGACTGAGTTTGT
CTGCTGTGGGGGATCTAAAAGTGGACGATAGCTGGATCCCCACTCTATTGGAACTAGTCA
AAGCCCGGCATGGCCCACCTACTTCTTTTGTGGTCCTCCTCCACCG
Restriction Sites NotI-NotI     
ACCN NM_000702
Insert Size 5000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000702.2, NP_000693.1
RefSeq Size 5468 bp
RefSeq ORF 3063 bp
Locus ID 477
Cytogenetics 1q23.2
Domains E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C
Protein Families Druggable Genome, Transmembrane
Protein Pathways Cardiac muscle contraction
Gene Summary 'The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 2 subunit. Mutations in this gene result in familial basilar or hemiplegic migraines, and in a rare syndrome known as alternating hemiplegia of childhood. [provided by RefSeq, Oct 2008]'

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