hCG (CGA) (NM_000735) Human Untagged Clone

CAT#: SC119734

CGA (untagged)-Human glycoprotein hormones, alpha polypeptide (CGA)


  "NM_000735" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CGA
Synonyms CG-ALPHA; FSHA; GPA1; GPHa; GPHA1; HCG; LHA; TSHA
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119734 sequence for NM_000735 edited (data generated by NextGen Sequencing)
ATGGATTACTACAGAAAATATGCAGCTATCTTTCTGGTCACATTGTCGGTGTTTCTGCAT
GTTCTCCATTCCGCTCCTGATGTGCAGGATTGCCCAGAATGCACGCTACAGGAAAACCCA
TTCTTCTCCCAGCCGGGTGCCCCAATACTTCAGTGCATGGGCTGCTGCTTCTCTAGAGCA
TATCCCACTCCACTAAGGTCCAAGAAGACGATGTTGGTCCAAAAGAACGTCACCTCAGAG
TCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCACAGTAATGGGGGGTTTCAAAGTG
GAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACAAATCTTAA

Clone variation with respect to NM_000735.2
>OriGene 5' read for NM_000735 unedited
GCTGGATTTAGGNAATCCGNATTTCACTATAGGGCGAGCCGCGCAATTCGGCACCAGNAC
GAAGCTAAAATCCCTCTTCGGATCCACAGTCAACCGCCCTGAACACTCCTGCAAAAAGCC
CAGAGAAAGGAGCGCCATGGATTACTACAGAAAATATGCAGCTATCTTTCTGGTCACATT
GTCGGTGTTTCTGCATGTTCTCCATTCCGCTCCTGTATGTTCAGGATTGCCCAGAATGCA
CGCTACAGGAAAACCCATTCTTCTCCCAGCCGGGTGCCCCAATACTTCAGTGCATGGGCT
GCTGCTTCTCTAGAGCATATCCCACTCCACTAAGGTCCAAGAAGACGATGTTGGTCCAAA
AGAACGTCACCTCAGAGTCCACTTGCTGTGTAGCTAAATCATATAACAGGGTCACAGTAA
TGGGGGGTTTCAAAGTGGAGAACCACACGGCGTGCCACTGCAGTACTTGTTATTATCACA
AATCTTAAATGTTTTACCAAGTGCTGTCTTGATGACTGCTGATTTTCTGGAATGGAAAAT
TAAGTTGTTTAGTGTTTATGGCTTTGTGAGATAAAACTCTCCTTTTCCTTACCATACCAC
TTTGACACGCTTCAAGGATATACTGCAGCTTTACTGCCTTCCTCCTTATCCTACAGTACA
ATCAGCAGTCTAGTTCTTTTCATTTGGGATGAATACAGCATTTAGCTTGGTCCACTGCAA
ATAAAGCCTTTTTAATCATCAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCNGCCGCG
GTCATAGCTGTTTCCCTGACAGATCCCGGGTGGCATCCCTGTGACCCTCCCCAATGCCTT
TCTGGGCCTGGAAGTGCCCCTCCAGTGCCAACAAACTTGGGCCTATAAATAAAGTGCATC
ATTTTGNCTGACAAGGTGTCCCTCTTAAATTATGGGTTGAGAGGGTTGTGTTGAAAACCA
GGGCAAATTTGGAAAAAA
Restriction Sites NotI-NotI     
ACCN NM_000735
Insert Size 700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000735.2, NP_000726.1
RefSeq Size 704 bp
RefSeq ORF 351 bp
Locus ID 1081
Cytogenetics 6q14.3
Domains GHA
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways Autoimmune thyroid disease, GnRH signaling pathway, Neuroactive ligand-receptor interaction
Gene Summary 'The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]'
Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

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