NCF4 (NM_000631) Human Untagged Clone

CAT#: SC119769

NCF4 (untagged)-Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1


  "NM_000631" in other vectors (6)

Reconstitution Protocol

USD 580.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NCF4
Synonyms CGD3; NCF; P40PHOX; SH3PXD4
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119769 sequence for NM_000631 edited (data generated by NextGen Sequencing)
ATGGCTGTGGCCCAGCAGCTGCGGGCCGAGAGTGACTTTGAACAGCTTCCGGATGATGTT
GCCATCTCGGCCAACATTGCTGACATCGAGGAGAAGAGAGGCTTCACCAGCCACTTTGTT
TTCGTCATCGAGGTGAAGACAAAAGGAGGATCCAAGTACCTCATCTACCGCCGCTACCGC
CAGTTCCATGCTTTGCAGAGCAAGCTGGAGGAGCGCTTCGGGCCAGACAGCAAGAGCAGT
GCCCTGGCCTGTACCCTGCCCACACTCCCAGCCAAAGTCTACGTGGGTGTGAAACAGGAG
ATCGCCGAGATGCGGATACCTGCCCTCAACGCCTACATGAAGAGCCTGCTCAGCCTGCCG
GTCTGGGTGCTGATGGATGAGGACGTCCGGATCTTCTTTTACCAGTCGCCCTATGACTCA
GAGCAGGTGCCCCAGGCACTCCGCCGGCTCCGCCCGCGCACCCGGAAAGTCAAGAGCGTG
TCCCCACAGGGCAACAGCGTTGACCGCATGGCAGCTCCGAGAGCAGAGGCTCTATTTGAC
TTCACTGGAAACAGCAAACTGGAGCTGAATTTCAAAGCTGGAGATGTGATCTTCCTCCTC
AGTCGGATCAACAAAGACTGGCTGGAGGGCACTGTCCGGGGAGCCACGGGCATCTTCCCT
CTCTCCTTCGTGAAGATCCTCAAAGACTTCCCTGAGGAGGACGACCCCACCAACTGGCTG
CGTTGCTACTACTACGAAGACACCATCAGCACCATCAAGGACATCGCGGTGGAGGAAGAT
CTCAGCAGCACTCCCCTATTGAAAGACCTGCTGGAGCTCACAAGGCGGGAGTTCCAGAGA
GAGGACATAGCTCTGAATTACCGGGACGCTGAGGGGGATCTGGTTCGGCTGCTGTCGGAT
GAGGACGTAGCGCTCATGGTGCGGCAGGCTCGTGGCCTCCCCTCCCAGAAGCGCCTCTTC
CCCTGGAAGCTGCACATCACGCAGAAGGACAACTACAGGGTCTACAACACGATGCCATGA

Clone variation with respect to NM_000631.4
>OriGene 5' read for NM_000631 unedited
AGGAATTTGTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGCCCAAGGCAG
CTCCTGGGGACTCCCAGACCACAGGCTGAGACGAGACGCAGGGTGGCTGGAGGAAGTGAG
AGGTGAACTCAGCCTGGGACTGGCTGGGCGAGACTCTCCACCTGCTCCCTGGGACCATCG
CCCACCATGGCTGTGGCCCAGCAGCTGCGGGCCGAGAGTGACTTTGAACAGCTTCCGGAT
GATGTTGCCATCTCGGCCAACATTGCTGACATCGAGGAGAAGAGAGGCTTCACCAGCCAC
TTTGTTTTCGTCATCGAGGTGAAGACAAAAGGAGGATCCAAGTACCTCATCTACCGCCGC
TACCGCCAGTTCCATGCTTTGCAGAGCAAGCTGGAGGAGCGCTTCGGGCCAGACAGCAAG
AGCAGTGCCCTGGCCTGTACCCTGCCCACACTCCCAGCCAAAGTCTACGTGGGTGTGAAA
CAGGAGATCGCCGAGATGCGGATACCTGCCCTCAACGCCTACATGAAGAGCCTGCTCAGC
CTGCCGGTCTGGGTGCTGATGGATGAGGACGTCCGGATCTTCTTTTACCAGTCGCCCTAT
GACTCAGAGCAGGTGCCCCAGGCACTCCGCCGGCTCCGCCCGCGCACCCGGAAAGTCAAG
AGCGTGTCCCCACAGGGCAACAGCGTTGACCGCATGGCAGCTCCGAGAGCAGAGGCTCTA
TTTGACTTCACTGGAAACAGCAAACTGGAGCTGAATTTAAAGCTGGAGATGTGATCTTCC
TNCTCAGTCGNATCAACNAGACTGGCTGGAGGGCACTGTCCGGNNGAGCCACGGCATCTT
CCCTCTCTTCTTTCGTGAAGAACTCAAAGACTTCCCTGAGAGGACGACCCCNACACTGGC
TGCGNTGCTACTACTACGAAGACACATCAGCACATCAGGACATCGCGGTGGAGGAAGACT
CACAGACTNCCCTATGAANGACTGCG
>OriGene 3' read for NM_000631 unedited
TTTTTTTTTTTTTTTTTCCCCTTTCAAAGGAATGGTTTATTTAAATCAGATGTAAATAGT
AGAAAGAGACAGATTACAAGCCCCACGGGTCCATTAGCCTCAAAAAAGGAAGTCTGCCCA
TCCTCCCAGGTTTTCCTGGTCCCAATCTCCTCTGAGAGCTGAAGGTTTTTGCTGGTGTCC
CCTCACTGCTCCAGGGACACCGTCAGCTCATGGCATCGTGTTGTAGACCCTGTAGTTGTC
CTTCTGCGTGATGTGCAGCTTCCAGGGGAAGAGGCGCTTCTGGGAGGGGAGGCCACGAGC
CTGCCGCACCATGAGCGCTACGTCCTCATCCGACAGCAGCCGAACCAGATCCCCCTCAGC
GTCCCGGTAATTCAGAGCTATGTCCTCTCTCTGGAACTCCCGCCTTGTGAGCTCCAGCAG
GTCTTTCAATAGGGGAGTGCTGCTGAGATCTTCCTCCACCGCGATGTCCTTGATGGTGCT
GATGGTGTCTTCGTAGTAGTAGCAACGCAGCCAGTTGGTGGGGTCGTCCTCCTCAGGGAA
GTCTTTGAGGATCTTCA
Restriction Sites NotI-NotI     
ACCN NM_000631
Insert Size 1460 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000631.3, NP_000622.2
RefSeq Size 1386 bp
RefSeq ORF 1020 bp
Locus ID 4689
Cytogenetics 22q12.3
Domains PB1, SH3, PX
Protein Pathways Leukocyte transendothelial migration
Gene Summary 'The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) lacks an internal coding segment compared to variant 2, which leads to a translation frameshift. The resulting isoform (1) has a distinct and shorter C-terminus, as compared to isoform 2.

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