CD14 (NM_000591) Human Untagged Clone

CAT#: SC119773

CD14 (untagged)-Human CD14 molecule (CD14), transcript variant 1


  "NM_000591" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CD14
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119773 sequence for NM_000591 edited (data generated by NextGen Sequencing)
ATGGAGCGCGCGTCCTGCTTGTTGCTGCTGCTGCTGCCGCTGGTGCACGTCTCTGCGACC
ACGCCAGAACCTTGTGAGCTGGACGATGAAGATTTCCGCTGCGTCTGCAACTTCTCCGAA
CCTCAGCCCGACTGGTCCGAAGCCTTCCAGTGTGTGTCTGCAGTAGAGGTGGAGATCCAT
GCCGGCGGTCTCAACCTAGAGCCGTTTCTAAAGCGCGTCGATGCGGACGCCGACCCGCGG
CAGTATGCTGACACGGTCAAGGCTCTCCGCGTGCGGCGGCTCACAGTGGGAGCCGCACAG
GTTCCTGCTCAGCTACTGGTAGGCGCCCTGCGTGTGCTAGCGTACTCCCGCCTCAAGGAA
CTGACGCTCGAGGACCTAAAGATAACCGGCACCATGCCTCCGCTGCCTCTGGAAGCCACA
GGACTTGCACTTTCCAGCTTGCGCCTACGCAACGTGTCGTGGGCGACAGGGCGTTCTTGG
CTCGCCGAGCTGCAGCAGTGGCTCAAGCCAGGCCTCAAGGTACTGAGCATTGCCCAAGCA
CACTCGCCTGCCTTTTCCTGCGAACAGGTTCGCGCCTTCCCGGCCCTTACCAGCCTAGAC
CTGTCTGACAATCCTGGACTGGGCGAACGCGGACTGATGGCGGCTCTCTGTCCCCACAAG
TTCCCGGCCATCCAGAATCTAGCGCTGCGCAACACAGGAATGGAGACGCCCACAGGCGTG
TGCGCCGCACTGGCGGCGGCAGGTGTGCAGCCCCACAGCCTAGACCTCAGCCACAACTCG
CTGCGCGCCACCGTAAACCCTAGCGCTCCGAGATGCATGTGGTCCAGCGCCCTGAACTCC
CTCAATCTGTCGTTCGCTGGGCTGGAACAGGTGCCTAAAGGACTGCCAGCCAAGCTCAGA
GTGCTCGATCTCAGCTGCAACAGACTGAACAGGGCGCCGCAGCCTGACGAGCTGCCCGAG
GTGGATAACCTGACACTGGACGGGAATCCCTTCCTGGTCCCTGGAACTGCCCTCCCCCAC
GAGGGCTCAATGAACTCCGGCGTGGTCCCAGCCTGTGCACGTTCGACCCTGTCGGTGGGG
GTGTCGGGAACCCTGGTGCTGCTCCAAGGGGCCCGGGGCTTTGCCTAA

Clone variation with respect to NM_000591.3
>OriGene 5' read for NM_000591 unedited
CGACTCACTATAGGGCGGCCGCGAATTCGGCACCGAGGGAAGAGTTCACAAGTGTGAAGC
CTGGAAGCCGGCGGGTGCCGCTGTGTAGGAAAGAAGCTAAAGCACTTCCAGAGCCTGTCC
GGAGCTCAGAGGTTCGGAAGACTTATCGACCATGGAGCGCGCGTCCTGCTTGTTGCTGCT
GCTGCTGCCGCTGGTGCACGTCTCTGCGACCACGCCAGAACCTTGTGAGCTGGACGATGA
AGATTTCCGCTGCGTCTGCAACTTCTCCGAACCTCAGCCCGACTGGTCCGAAGCCTTCCA
GTGTGTGTCTGCAGTAGAGGTGGAGATCCATGCCGGCGGTCTCAACCTAGAGCCGTTTCT
AAAGCGCGTCGATGCGGACGCCGACCCGCGGCAGTATGCTGACACGGTCAAGGCTCTCCG
CGTGCGGCGGCTCACAGTGGGAGCCGCACAGGTTCCTGCTCAGCTACTGGTAGGCGCCCT
GCGTGTGCTAGCGTACTCCCGCCTCAAGGAACTGACGCTCGAGGACCTANAGATAACCGG
CACCATGCCTCCGCTGCCTCTGGAAGCCACAGGACTTGCACTTTCCAGCTTGCGCCTACG
CAACGTGTCGTGGGCGACAGGGCGTTCTTGGCTCGCCGAGCTGCAGCAGTGGCTCAAGCC
AGCCCTCAGGTACTGAGCATTGCCCAGCACACTCGCCTGCCTTTTTCTGCGAACAGTTTC
GCGCCCTTCCCGGCCCTTACCAGNCTAGACCTGTCCTGACATCCTGGACTGGGCGAAACG
CGGACTGATGGCNGNTCCTCTGTCCCCACCAGTTCCCGGCCATCCAGAATCTAGCGCTGC
GCAACACAGGGATTGAAGACGCCCACAGGGTGTTCCGCCGACCTGGCCGNGTAAGGTGTG
CACCCCCCAAGCCTAACCTCAGCCCAACTGGCTGCCGCCACCGTAAACCTACCGTCGAGA
AGCTGGGCCCACCGCCTAACTCCTAAT
>OriGene 3' read for NM_000591 unedited
CCGGTNCGAAAAGTCCTCAACGTCCTGACGGGACTCCCCTGAACCAAGCAGTTTGAGTCC
ATTCATTATTCTGTCTTGGATCTTAGGCAAAGCCCCGGGCCCCTTGGAGCAGCACCAGGG
TTCCCGACACCCCCACCGACAGGGTCGAACGTGCACAGGCTGGGACCACGCCTGATTTTA
TTGAGCCCTCGTGGGGGAGGGCAGTTCCAGGGACCAGGAAGGGATTCCCGTCCAGTGTCA
GGTTATCCACCTCGGGCAGCTCGTCAGGCTGCGGCGCCCTGTTCAGTCTGTTGCAGCTGA
GATCGAGCACTCTGAGCTTGGCTGGCAGTCCTTTAGGCACCTGTTCCAGCCCAGCGAACG
ACAGATTGAGGGAGTTCAGGGCGCTGGACCACATGCATCTCGGAGCGCTAGGGTTTACGG
TGGCGCGCAGCGAGTTGTGGCTGAAGTCTAGGCTGTGGGGCTGCACACCTGCCGCCGCCA
GTGCGGCGCACACGCCTGTGGGCGTCTCCATTCCTGTGTTGCGCAGCGCTAGATTCTGGA
TGGCCGGGAACTTGTGGGGACAGAGAGCCGCCATCAGTCCGCGTTCGCCCAGTCCAGGAT
TGTCAGACAGGTCTAGGCTGGTAAGGGCCGGGAAGGCGCGAACCTGTTCGCAGGATAAGG
CAGGCGAGTGTGCTTGGGCAATGCTCAGTACCTTGAGGCCTGGCTTGAGCCACTGCTGCA
GCTCGGCGAGCCAAGAACGCCCTGTCGCCCACGACACGTTGCGTATGCGCAAGCTGGAAA
GTGCAAGTCCTGTGGCTTCCANAGCAGCGGAGGCATGGTGCCGGTTATCTTTTAGTCCTC
GAGCGTCAGTTCCTTTGAGCG
Restriction Sites ECoRI-NOT     
ACCN NM_000591
Insert Size 1330 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000591.1, NP_000582.1
RefSeq Size 1367 bp
RefSeq ORF 1128 bp
Locus ID 929
Cytogenetics 5q31.3
Domains LRR
Protein Families Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Hematopoietic cell lineage, MAPK signaling pathway, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Toll-like receptor signaling pathway
Gene Summary 'The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide, and to viruses. This gene has been identified as a target candidate in the treatment of SARS-CoV-2-infected patients to potentially lessen or inhibit a severe inflammatory response. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Aug 2020]'
Transcript Variant: This variant (1) represents the predominant transcript. Variants 1-4 encode the same protein.

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