CD14 (NM_000591) Human Untagged Clone

CAT#: SC119773

CD14 (untagged)-Human CD14 molecule (CD14), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD14
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119773 sequence for NM_000591 edited (data generated by NextGen Sequencing)
  ATGGAGCGCGCGTCCTGCTTGTTGCTGCTGCTGCTGCCGCTGGTGCACGTCTCTGCGACC
  ACGCCAGAACCTTGTGAGCTGGACGATGAAGATTTCCGCTGCGTCTGCAACTTCTCCGAA
  CCTCAGCCCGACTGGTCCGAAGCCTTCCAGTGTGTGTCTGCAGTAGAGGTGGAGATCCAT
  GCCGGCGGTCTCAACCTAGAGCCGTTTCTAAAGCGCGTCGATGCGGACGCCGACCCGCGG
  CAGTATGCTGACACGGTCAAGGCTCTCCGCGTGCGGCGGCTCACAGTGGGAGCCGCACAG
  GTTCCTGCTCAGCTACTGGTAGGCGCCCTGCGTGTGCTAGCGTACTCCCGCCTCAAGGAA
  CTGACGCTCGAGGACCTAAAGATAACCGGCACCATGCCTCCGCTGCCTCTGGAAGCCACA
  GGACTTGCACTTTCCAGCTTGCGCCTACGCAACGTGTCGTGGGCGACAGGGCGTTCTTGG
  CTCGCCGAGCTGCAGCAGTGGCTCAAGCCAGGCCTCAAGGTACTGAGCATTGCCCAAGCA
  CACTCGCCTGCCTTTTCCTGCGAACAGGTTCGCGCCTTCCCGGCCCTTACCAGCCTAGAC
  CTGTCTGACAATCCTGGACTGGGCGAACGCGGACTGATGGCGGCTCTCTGTCCCCACAAG
  TTCCCGGCCATCCAGAATCTAGCGCTGCGCAACACAGGAATGGAGACGCCCACAGGCGTG
  TGCGCCGCACTGGCGGCGGCAGGTGTGCAGCCCCACAGCCTAGACCTCAGCCACAACTCG
  CTGCGCGCCACCGTAAACCCTAGCGCTCCGAGATGCATGTGGTCCAGCGCCCTGAACTCC
  CTCAATCTGTCGTTCGCTGGGCTGGAACAGGTGCCTAAAGGACTGCCAGCCAAGCTCAGA
  GTGCTCGATCTCAGCTGCAACAGACTGAACAGGGCGCCGCAGCCTGACGAGCTGCCCGAG
  GTGGATAACCTGACACTGGACGGGAATCCCTTCCTGGTCCCTGGAACTGCCCTCCCCCAC
  GAGGGCTCAATGAACTCCGGCGTGGTCCCAGCCTGTGCACGTTCGACCCTGTCGGTGGGG
  GTGTCGGGAACCCTGGTGCTGCTCCAAGGGGCCCGGGGCTTTGCCTAA
  
  Clone variation with respect to NM_000591.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000591 unedited
  CGACTCACTATAGGGCGGCCGCGAATTCGGCACCGAGGGAAGAGTTCACAAGTGTGAAGC
  CTGGAAGCCGGCGGGTGCCGCTGTGTAGGAAAGAAGCTAAAGCACTTCCAGAGCCTGTCC
  GGAGCTCAGAGGTTCGGAAGACTTATCGACCATGGAGCGCGCGTCCTGCTTGTTGCTGCT
  GCTGCTGCCGCTGGTGCACGTCTCTGCGACCACGCCAGAACCTTGTGAGCTGGACGATGA
  AGATTTCCGCTGCGTCTGCAACTTCTCCGAACCTCAGCCCGACTGGTCCGAAGCCTTCCA
  GTGTGTGTCTGCAGTAGAGGTGGAGATCCATGCCGGCGGTCTCAACCTAGAGCCGTTTCT
  AAAGCGCGTCGATGCGGACGCCGACCCGCGGCAGTATGCTGACACGGTCAAGGCTCTCCG
  CGTGCGGCGGCTCACAGTGGGAGCCGCACAGGTTCCTGCTCAGCTACTGGTAGGCGCCCT
  GCGTGTGCTAGCGTACTCCCGCCTCAAGGAACTGACGCTCGAGGACCTANAGATAACCGG
  CACCATGCCTCCGCTGCCTCTGGAAGCCACAGGACTTGCACTTTCCAGCTTGCGCCTACG
  CAACGTGTCGTGGGCGACAGGGCGTTCTTGGCTCGCCGAGCTGCAGCAGTGGCTCAAGCC
  AGCCCTCAGGTACTGAGCATTGCCCAGCACACTCGCCTGCCTTTTTCTGCGAACAGTTTC
  GCGCCCTTCCCGGCCCTTACCAGNCTAGACCTGTCCTGACATCCTGGACTGGGCGAAACG
  CGGACTGATGGCNGNTCCTCTGTCCCCACCAGTTCCCGGCCATCCAGAATCTAGCGCTGC
  GCAACACAGGGATTGAAGACGCCCACAGGGTGTTCCGCCGACCTGGCCGNGTAAGGTGTG
  CACCCCCCAAGCCTAACCTCAGCCCAACTGGCTGCCGCCACCGTAAACCTACCGTCGAGA
  AGCTGGGCCCACCGCCTAACTCCTAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000591 unedited
  CCGGTNCGAAAAGTCCTCAACGTCCTGACGGGACTCCCCTGAACCAAGCAGTTTGAGTCC
  ATTCATTATTCTGTCTTGGATCTTAGGCAAAGCCCCGGGCCCCTTGGAGCAGCACCAGGG
  TTCCCGACACCCCCACCGACAGGGTCGAACGTGCACAGGCTGGGACCACGCCTGATTTTA
  TTGAGCCCTCGTGGGGGAGGGCAGTTCCAGGGACCAGGAAGGGATTCCCGTCCAGTGTCA
  GGTTATCCACCTCGGGCAGCTCGTCAGGCTGCGGCGCCCTGTTCAGTCTGTTGCAGCTGA
  GATCGAGCACTCTGAGCTTGGCTGGCAGTCCTTTAGGCACCTGTTCCAGCCCAGCGAACG
  ACAGATTGAGGGAGTTCAGGGCGCTGGACCACATGCATCTCGGAGCGCTAGGGTTTACGG
  TGGCGCGCAGCGAGTTGTGGCTGAAGTCTAGGCTGTGGGGCTGCACACCTGCCGCCGCCA
  GTGCGGCGCACACGCCTGTGGGCGTCTCCATTCCTGTGTTGCGCAGCGCTAGATTCTGGA
  TGGCCGGGAACTTGTGGGGACAGAGAGCCGCCATCAGTCCGCGTTCGCCCAGTCCAGGAT
  TGTCAGACAGGTCTAGGCTGGTAAGGGCCGGGAAGGCGCGAACCTGTTCGCAGGATAAGG
  CAGGCGAGTGTGCTTGGGCAATGCTCAGTACCTTGAGGCCTGGCTTGAGCCACTGCTGCA
  GCTCGGCGAGCCAAGAACGCCCTGTCGCCCACGACACGTTGCGTATGCGCAAGCTGGAAA
  GTGCAAGTCCTGTGGCTTCCANAGCAGCGGAGGCATGGTGCCGGTTATCTTTTAGTCCTC
  GAGCGTCAGTTCCTTTGAGCG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_000591
• Insert Size: 1330 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000591.1, NP_000582.1
• RefSeq Size: 1367 bp
• RefSeq ORF: 1128 bp
• Locus ID: 929
• Cytogenetics: 5q31.3
• Domains: LRR
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Hematopoietic cell lineage, MAPK signaling pathway, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Toll-like receptor signaling pathway
• Gene Summary: 'The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide, and to viruses. This gene has been identified as a target candidate in the treatment of SARS-CoV-2-infected patients to potentially lessen or inhibit a severe inflammatory response. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (1) represents the predominant transcript. Variants 1-4 encode the same protein.