IGFBP2 (NM_000597) Human Untagged Clone

CAT#: SC119778

IGFBP2 (untagged)-Human insulin-like growth factor binding protein 2, 36kDa (IGFBP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IGFBP2
• Synonyms: IBP2; IGF-BP53
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000597, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGCCGAGAGTGGGCTGCCCCGCGCTGCCGCTGCCGCCGCCGCCGCTGCTGCCGCTGCTGCCGCTGC
  TGCTGCTGCTACTGGGCGCGAGTGGCGGCGGCGGCGGGGCGCGCGCGGAGGTGCTGTTCCGCTGCCCGCC
  CTGCACACCCGAGCGCCTGGCCGCCTGCGGGCCCCCGCCGGTTGCGCCGCCCGCCGCGGTGGCCGCAGTG
  GCCGGAGGCGCCCGCATGCCATGCGCGGAGCTCGTCCGGGAGCCGGGCTGCGGCTGCTGCTCGGTGTGCG
  CCCGGCTGGAGGGCGAGGCGTGCGGCGTCTACACCCCGCGCTGCGGCCAGGGGCTGCGCTGCTATCCCCA
  CCCGGGCTCCGAGCTGCCCCTGCAGGCGCTGGTCATGGGCGAGGGCACTTGTGAGAAGCGCCGGGACGCC
  GAGTATGGCGCCAGCCCGGAGCAGGTTGCAGACAATGGCGATGACCACTCAGAAGGAGGCCTGGTGGAGA
  ACCACGTGGACAGCACCATGAACATGTTGGGCGGGGGAGGCAGTGCTGGCCGGAAGCCCCTCAAGTCGGG
  TATGAAGGAGCTGGCCGTGTTCCGGGAGAAGGTCACTGAGCAGCACCGGCAGATGGGCAAGGGTGGCAAG
  CATCACCTTGGCCTGGAGGAGCCCAAGAAGCTGCGACCACCCCCTGCCAGGACTCCCTGCCAACAGGAAC
  TGGACCAGGTCCTGGAGCGGATCTCCACCATGCGCCTTCCGGATGAGCGGGGCCCTCTGGAGCACCTCTA
  CTCCCTGCACATCCCCAACTGTGACAAGCATGGCCTGTACAACCTCAAACAGTGCAAGATGTCTCTGAAC
  GGGCAGCGTGGGGAGTGCTGGTGTGTGAACCCCAACACCGGGAAGCTGATCCAGGGAGCCCCCACCATCC
  GGGGGGACCCCGAGTGTCATCTCTTCTACAATGAGCAGCAGGAGGCTCGCGGGGTGCACACCCAGCGGAT
  GCAGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000597 unedited
  GGGTTGAGGATTTGTAAACTTTCATATAGGCGGCCCGCGAATCGGCACGAGCTCGCAGGG
  CCGTGCCACCTGCCCGCCCGCCCGCTCGCTCGCTCGCCCGCCGCGCCGCGCCGCGCTGCC
  GACCGCCAGCATGCTGCCGAGAGTGGGCTGCCCCGCGCTGCCGCTGCCGCCGCCGCCGCT
  GCTGCCGCTGCTGCCGCTGCTGCTGCTGCTACTGGGCGCGAGTGGCGGCGGCGGCGGGGC
  GCGCGCGGAGGTGCTGTTCCGCTGCCCGCCCTGCACACCCGAGCGCCTGGCCGCCTGCGG
  GCCCCCGCCGGTTGCGCCGCCCGCCGCGGTGGCCGCAGTGGCCGGAGGCGCCCGCATGCC
  ATGCGCGGAGCTCGTCCGGGAGCCGGGCTGCGGCTGCTGCTCGGTGTGCGCCCGGCTGGA
  GGGCGAGGCGTGCGGCGTCTACACCCCGCGCTGCGGCCAGGGGCTGCGCTGCTATCCCCA
  CCCGGGCTCCGAGCTGCCCCTGCAGGCGCTGGTCATGGGCGAGGGCACTTGTGAGAAGCG
  CCGGGACGCCGAGTATGGCGCCAGCCCGGAGCAGGTTGCAGACAATGGCGATGACCACTC
  AGAAGGAGGCCTGGTGGAGAACCACGTGGACAGCACCATGAACATGTTGGGCGGCGGAGG
  CAGTGCTGGCCGGAAGCCCCTCAAGTCGGGTATGAAGGAGCTGGCCGTGTTCCGGGAGTA
  GGTCACTGAGCAGCACCGGCAGATGGGCAAGGGTGGCAAGCATCACCTTGGCCTGGAGGA
  GCCCAAGAAGCTGCGACCACCCCCTGCCAGGACTCCCTGCCAACAGGACCTGGACCAGGT
  CCTGGGAACGGGATCCCCACCATGCCCCCTTCCGGATGAGCCGGGGCCCTCTTGAGGCAC
  CTCTACTTCCTGGACATTCCCAAACGCGACAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000597 unedited
  GTGTGACCGAGCCTCAGTCGAGGATCGAGTAAAATTCTTTTTTTTTTTACTTTTCCTTCC
  TTTAATCTTATGCAAAAGGGACACAGGGGTTCAAAAATAAAAATTTCTCTTCCCCCTCCC
  CAAACCTGTACCCCAGCTCCCCGACCACAACCCCCTTCCTCCCCCGGGGAAAGCAAGAAG
  GAGCAGGTGTGGCATCTGCAGCTGGGAATAGAGTGGCCGGGGAGGTGCCGAGCTCGGTGC
  TGGTCTCTTTCCAAATATAAATACGTGTGTCAGAACTGGAAAATCCTCCAGCACCCACCA
  CCCAAGCACTCTCCGTTTTCTGCCGGTGTTTGGAGAGGGGCGGGGGGCAGGGGCGCCAGG
  CACCGGCTGGCTGCGGTCTACTGCATCCGCTGGGTGTGCACCCCGCGAGCCTCCTGCTGC
  TCATTGTACAAGAGATGACACTCGGGGTCCCCCCGGATGGTGGGGGCTCCCTGGATCAGC
  TTCCCGGTGTTGGGGTTTACACACCAGTACTCCCCACGCTGCCCCGTCACAGACATCTCG
  CACTGGTCGAGGGTCGCCAGGCCATGCCTGTCCCCCTCGGGGTGTGCCGGCAGTCCGGGC
  CCCACGCGCCCCCCGCCTCCGCACGGTCGCCGGTTTCTCTTCTCCTCCCGCCCGACGCCT
  GTAGTTGTGCCCTCCCCCGCTTGGCGGAGCCCCGCGCCCGTCGTTTCCGGGGCGGGGTGC
  GGCCCGGAGTGCCCGGACGCGCGGGCCGAGTCCTCGTGGCGCTCCGCGCGGCGCCCGCTT
  GCGTAGCCGTCGGTGTTTTCCCGCTTGTTTGTCACTCGCCCGGTAGCCCGCGCACGTGCC
  CTCCGCCCGGTGCCCGGGTTATTTTGCCGCCGCCCGCCGCGCTATCCCTCCGCTTTTTCA
  TCCGGGCACCCCGGGTTGCGTTTGTGGGTGTCAGGTCGCGAGCGCACGCTCTCCGTCGTT
  CGGCGCATCGCCCGCCGCGTCTTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000597
• Insert Size: 1440 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000597.2, NP_000588.2
• RefSeq Size: 1439 bp
• RefSeq ORF: 987 bp
• Locus ID: 3485
• Cytogenetics: 2q35
• Domains: thyroglobulin_1, IB
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein
• Gene Summary: 'The protein encoded by this gene is one of six similar proteins that bind insulin-like growth factors I and II (IGF-I and IGF-II). The encoded protein can be secreted into the bloodstream, where it binds IGF-I and IGF-II with high affinity, or it can remain intracellular, interacting with many different ligands. High expression levels of this protein promote the growth of several types of tumors and may be predictive of the chances of recovery of the patient. Several transcript variants, one encoding a secreted isoform and the others encoding nonsecreted isoforms, have been found for this gene. [provided by RefSeq, Sep 2015]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). CCDS Note: The coding region was updated to make it identical to the current human reference genome sequence.