IGFBP3 (NM_000598) Human Untagged Clone

CAT#: SC119779

IGFBP3 (untagged)-Human insulin-like growth factor binding protein 3 (IGFBP3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IGFBP3
• Synonyms: BP-53; IBP3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000598 edited
  GAATTCGGCACGAGGTGGGCGCTGAGGATCAGCCGCTTCCTGCCTGGATTCCACAGCTTC
  GCGCCGTGTACTGTCGCCCCATCCCTGCGCGCCCAGCCTGCCAAGCAGCGTGCCCCGGTT
  GCAGGCGTCATGCAGCGGGCGCGACCCACGCTCTGGGCCGCTGCGCTGACTCTGCTGGTG
  CTGCTCCGCGGGCCGCCGGTGGCGCGGGCTGGCGCGAGCTCGGGGGGCTTGGGTCCCGTG
  GTGCGCTGCGAGCCGTGCGACGCGCGTGCACTGGCCCAGTGCGCGCCTCCGCCCGCCGTG
  TGCGCGGAGCTGGTGCGCGAGCCGGGCTGCGGCTGCTGCCTGACGTGCGCACTGAGCGAG
  GGCCAGCCGTGCGGCATCTACACCGAGCGCTGTGGCTCCGGCCTTCGCTGCCAGCCGTCG
  CCCGACGAGGCGCGACCGCTGCAGGCGCTGCTGGACGGCCGCGGGCTCTGCGTCAACGCT
  AGTGCCGTCAGCCGCCTGCGCGCCTACCTGCTGCCAGCGCCGCCAGCTCCAGGAAATGCT
  AGTGAGTCGGAGGAAGACCGCAGCGCCGGCAGTGTGGAGAGCCCGTCCGTCTCCAGCACG
  CACCGGGTGTCTGATCCCAAGTTCCACCCCCTCCATTCAAAGATAATCATCATCAAGAAA
  GGGCATGCTAAAGACAGCCAGCGCTACAAAGTTGACTACGAGTCTCAGAGCACAGATACC
  CAGAACTTCTCCTCCGAGTCCAAGCGGGAGACAGAATATGGTCCCTGCCGTAGAGAAATG
  GAAGACACACTGAATCACCTGAAGTTCCTCAATGTGCTGAGTCCCAGGGGTGTACACATT
  CCCAACTGTGACAAGAAGGGATTTTATAAGAAAAAGCAGTGTCGCCCTTCCAAAGGCAGG
  AAGCGGGGCTTCTGCTGGTGTGTGGATAAGTATGGGCAGCCTCTCCCAGGCTACACCACC
  AAGGGGAAGGAGGACGTGCACTGCTACAGCATGCAGAGCAAGTAGACGCCTGCCGCAAGG
  TTAATGTGGAGCTCAAATATGCCTTATTTTGCACAAAAGACTGCCAAGGACATGACCAGC
  AGCTGGCTACAGCCTCGATTTATATTTCTGTTTGTGGTGAACTGATTTTTTTTAAACCAA
  AGTTTAGAAAGAGGTTTTTGAAATGCCTATGGTTTCTTTGAATGGTAAACTTGAGCATCT
  TTTCACTTTCCAGTAGTCAGCAAAGAGCAGTTTGAATTTTCTTGTCGCTTCCTATCAAAA
  TATTCAGAGACTCGAGCACAGCACCCAGACTTCATGCGCCCGTGGAATGCTCACCACATG
  TTGGTCGAAGCGGCCGACCACTGACTTTGTGACTTAGGCGGCTGTGTTGCCTATGTAGAG
  AACACGCTTCACCCCCACTCCCCGTACAGTGCGCACAGGCTTTATCGAGAATAGGAAAAC
  CTTTAAACCCCGGTCATCCGGACATCCCAACGCATGCTCCTGGAGCTCACAGCCTTCTGT
  GGTGTCATTTCTGAAACAAGGGCGTGGATCCCTCAACCAAGAAGAATGTTTATGTCTTCA
  AGTGACCTGTACTGCTTGGGGACTATTGGAGAAAATAAGGTGGAGTCCTACTTGTTTAAA
  AAATATGTATCTAAGAATGTTCTAGGGCACTCTGGGAACCTATAAAGGCAGGTATTTCGG
  GCCCTCCTCTTCAGGAATCTTCCTGAAGACATGGCCCAGTCGAAGGCCCAGGATGGCTTT
  TGCTGCGGCCCCGTGGGGTAGGAGGGACAGAGAGACAGGGAGAGTCAGCCTCCACATTCA
  GAGGCATCACAAGTAATGGCACAATTCTTCGGATGACTGCAGAAAATAGTGTTTTGTAGT
  TCAACAACTCAAGACGAAGCTTATTTCTGAGGATAAGCTCTTTAAAGGCAAAGCTTTATT
  TTCATCTCTCATCTTTTGTCCTCCTTAGCACAATGTAAAAAAGAATAGTAATATCAGAAC
  AGGAAGGAGGAATGGCTTGCTGGGGAGCCCATCCAGGACACTGGGAGCACATAGAGATTC
  ACCCATGTTTGTTGAACTTAGAGTCATTCTCATGCTTTTCTTTATAATTCACACATATAT
  GCAGAGAAGATATGTTCTTGTTAACATTGTATACAACATAGCCCCAAATATAGTAAGATC
  TATACTAGATAATCCTAGATGAAATGTTAGAGATGCTATATGATACAACTGTGGCCATGA
  CTGAGGAAAGGAGCTCACGCCCAGAGACTGGGCTGCTCTCCCGGAGGCCAAACCCAAGAA
  GGTCTGGCAAAGTCAGGCTCAGGGAGACTCTGCCCTGCTGCAGACCTCGGTGTGGACACA
  CGCTGCATAGAGCTCTCCTTGAAAACAGAGGGGTCTCAAGACATTCTGCCTACCTATTAG
  CTTTTCTTTATTTTTTTAACTTTTTGGGGGGAAAAGTATTTTTGAGAAGTTTGTCTTGCA
  ATGTATTTATAAATAGTAAATAAAGTTTTTACCATTAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000598 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGGGCGCTGAGGATC
  AGCCGCTTCCTGCCTGGATTCCACAGCTTCGCGCCGTGTACTGTCGCCCCATCCCTGCGC
  GCCCAGCCTGCCAAGCAGCGTGCCCCGGTTGCAGGCGTCATGCAGCGGGCGCGACCCACG
  CTCTGGGCCGCTGCGCTGACTCTGCTGGTGCTGCTCCGCGGGCCGCCGGTGGCGCGGGCT
  GGCGCGAGCTCGGGGGGCTTGGGTCCCGTGGTGCGCTGCGAGCCGTGCGACGCGCGTGCA
  CTGGCCCAGTGCGCGCCTCCGCCCGCCGTGTGCGCGGAGCTGGTGCGCGAGCCGGGCTGC
  GGCTGCTGCCTGACGTGCGCACTGAGCGAGGGCCAGCCGTGCGGCATCTACACCGAGCGC
  TGTGGCTCCGGCCTTCGCTGCCAGCCGTCGCCCGACGAGGCGCGACCGCTGCAGGCGCTG
  CTGGACGGCCGCGGGCTCTGCGTCAACGCTAGTGCCGTCAGCCGCCTGCGCGCCTACCTG
  CTGCCAGCGCCGCCAGCTCCAGGAAATGCTAGTGAGTCGGAGGAAGACCGCAGCGNCCGG
  CAGTGTGGAGAGCCCGTCCGTCTCCAGCACGCACCGGNGTGTCTGATCCCCAGTTCCACC
  CCCTCCATTCAAAGATAATCATCATCAAGAAAGGGCATGCTAAAGACAGCCAGCGCTACA
  AAGTTGACTACGAGTCTCAGAGCACAGATACCCAGAACTTNCTCTNCGAGTNCAGCGGGA
  GACGGAATATGGTCCCTGGCGTAGAAAAATGGAGAACACTGNATCACCTGAGTNCTCATG
  GGCTGATCCCCAGGGGTGACCATTCCCACTGGACANAAAGGGATTATAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000598 unedited
  CGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTAATGGTAAAAACTTTATTT
  ACTATTTATAAATACATTGCAAGACAAACTTCTCAAAAATACTTTTCCCCCCAAAAAGTT
  AAAAAAATAAAGAAAAGCTAATAGGTAGGCAGAATGTCTTGAGACCCCTCTGTTTTCAAG
  GAGAGCTCTATGCAGCGTGTGTCCACACCGAGGTCTGCAGCAGGGCAGAGTCTCCCTGAA
  CCTGACTTTTGCCAGACCCCTTTTTGGGTTGGGCCTCCGGGGAAAACACCCCCATCTTTT
  GGGGGGGGACCCTCTTTTCCTTAATTTGGGGCCCACGTGGGTTTAATATAGCCCCTTTTA
  ACACTTCTCCCTCGGAATATTCTCCACTAAAACCCTTCTATTTTTTGGGGGGGTTTTTTG
  GGTTACCATGGGTTAAAAAAAAACCTTTTTTTTTGGTTATTTTTTTGGGGGGGTTTTATA
  AAAAAAAGTGTTGAAGTTTCTCTTTTTCACCACCCACGGGGGTTTCTCTTTTTCCCCCCC
  CGCGCGGGGGGGGGGCCCCACAAAAACCCCCTCTTCGTGGGGGGGAAAAATATTTTTTTT
  TTTCCTCGGCGGGGGGGGGCGCTTAAAAGAAAAAAAAAATCCGTCTCTCTGCTCTTACTC
  TCCCACCCAAAACCTTTTTGTTGGTGGGGGGAAACAACCACACAAATTCTTTTTTTTCCA
  AAACACGCAACACCCTCCCTCTCCCCTTTTCAACAGACCTGAGCTTTCTTTTCTTTTCTT
  TCCCCCCCCCCCCCCCGCCTCATACTTCAATACTCTCTTGCTGGCTCCTTCTTCTTTCTT
  CCGCCCCTTCCTCGTCTCTCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000598
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000598.4, NP_000589.2
• RefSeq Size: 2620 bp
• RefSeq ORF: 876 bp
• Locus ID: 3486
• Cytogenetics: 7p12.3
• Domains: thyroglobulin_1, IB
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: p53 signaling pathway
• Gene Summary: 'This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in a shorter protein (isoform b).