SDF1 (CXCL12) (NM_000609) Human Untagged Clone

CAT#: SC119783

CXCL12 (untagged)-Human chemokine (C-X-C motif) ligand 12 (CXCL12), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CXCL12
• Synonyms: IRH; PBSF; SCYB12; SDF1; TLSF; TPAR1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000609 edited
  GAATTCGGCACGAGGGCTCTGCCTCAGCGACGGGAAGCCCGTCAGCCTGAGCTACAGATG
  CCCATGCCGATTCTTCGAAAGCCATGTTGCCAGAGCCAACGTCAAGCATCTCAAAATTCT
  CAACACTCCAAACTGTGCCCTTCAGATTGTAGCCCGGCTGAAGAACAACAACAGACAAGT
  GTGCATTGACCCGAAGCTAAAGTGGATTCAGGAGTACCTGGAGAAAGCTTTAAACAAGTA
  AGCACAACAGCCAAAAAGGACTTTCCGCTAGACCCACTCGAGGAAAACTAAAACCTTGTG
  AGAGATGAAAGGGCAAAGACGTGGGGGAGAGGGGGCCTTAACCATGAGGACCAGGTGTGT
  GTGTGGGGTGGGCACATTGATCTGGGATCGGGCCTGAGGTTTGCCAGCATTTAGACCCTG
  CATTTATAGCATACGGTATGATATTGCAGCTTATATTCATCCATGCCCTGTACCTGTGCA
  CGTTGGAACTTTTATTACTGGGGTTTTTCTAAGAAAGAAATTGTATTATCAACAGCATTT
  TCAAGCAGTTAGTTCCTTCATGATCATCACAATCATCATCATTCTCATTCTCATTTTTTA
  AATCAACGAGTACTTCAAGATCTGAATTTGGCTTGTTTGGAGCATCTCCTCTGCTCCCCT
  GGGGAGTCTGGGCACAGTCAGGTGGTGGCTTAACAGGGAGCTGGAAAAAGTGTCCTTTCT
  TCAGACACTGAGGCTCCCGCAGCAGCGCCCCTCCCAAGAGGAAGGCCTCTGTGGCACTCA
  GATACCGACTGGGGCTGGGCGCCGCCACTGCCTTCACCTCCTCTTTCAACCTCAGTGATT
  GGCTCTGTGGGCTCCATGTAGAAGCCACTATTACTGGGACTGTGCTCAGAGACCCCTCTC
  CCAGCTATTCCTACTCTCTCCCCGACTCCGAGAGCATGCTTAATCTTGCTTCTGCTTCTC
  ATTTCTGTAGCCTGAXXXXXXXXXXXXXXXXXXXXXXXCTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000609 unedited
  GGGTTCACGATGTTGTAACACGATTTACTATAGGGCGGCCGCGCAATTCGCACGAGGGTC
  AGCCGCATTGCCCGCTCGGCGTCCGGCCCCCGACCCGCGCTCGTCCGCCCGCCCGCCCGC
  CCGCCCGCGCCATGAACGCCAAGGTCGTGGTCGTGCTGGTCCTCGTGCTGACCGCGCTCT
  GCCTCAGCGACGGGAAGCCCGTCAGCCTGAGCTACTGATGCCCATGCCGATTCTTCGAAA
  GCCATGTTGCCAGAGCCAACGTCAAGCATCTCAAAATTCTCAACACTCCAAACTGTGCCC
  TTCAGATTGTAGCCCGGCTGAAGAACAACAACAGACAAGTGTGCATTGACCCGAAGCTAA
  AGTGGATTCAGGAGTACCTGGAGAAAGCTTTAAACAAGAGGTTCAAGATGTGAGAGGGTC
  AGACGCCTGAGGAACCCTTACAGTAGGAGCCCAGCTCTGAAACCAGTGTTAGGGAAGGGC
  CTGCCACAGCCTCCCCTGCCAGGGCAGGGCCCCAGGCATTGCCAAGGGCTTTGTTTTGCA
  CACTTTGCCCATATTTTCACCATTTGATTATGTAGCAAAATATATGACATTTATTTTTCA
  TTTAGTTCGATTATTTCAGTGTCACTGGCGACACGTAGCAGCTTACACTAAGGCCATTAT
  TGTACTTGCCCTTATTAGAGTGCCCTTCCACGGAGCCACTCCTTTGACTCAGGGCTCCTG
  GGTTTCGTACTCTCTGAACTGTGCACGTGGAGAGACTGGGCTTCAGGCAGCCTGGCCCCA
  TTGTCAGCCCTAGGGTGGAAACCCCCCAAAAGGACACCTCGGGGTGCCCGGAACCATTCA
  ACCTGGGCATACCTATTCAAGGTCTCTCTTCGTGGCCAGGATGGGGGCAGGCCCCTGCGA
  AGCTCCCCCCCTCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000609 unedited
  TGGACCGCGGCCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACATTTGAGTACA
  GAACTTTATTAGGAAAATTATAAGTGCATATACATTTTTTCATTGGTATGACTTCAAAGT
  AGAAATTGTTGTTTCTCAAAGAGTTGTTTCTTATTTATTTTTTAAAGCACGCTGCGTATA
  GGAATTGGCCTTCTCCTGGACCATTTTCACATTATCTGGGAGATGCAATTGAAACAATAC
  AAAATACAGTATTCATCACATTTAACACTGAATCATTCACTGCTCAGAGACTGAGAGTCC
  AGCGAGGTTGCAAAATAACAAATTTTCTACATGGTTTTATCAGTGGTCTGCTTAGGGGAT
  TTGGAAGTTTCTAGGGAATATTTCCCAGGAATGGGGCTCCTTCAGAATGAGTCAGGGAAG
  CTCTGTAGAGGGATCAGAGCACTCAAATTCTCATCCAGCTGGAAAAGGGTGTAGCACCTG
  GGAAAGGCGATCAACTCCCAGTCAGGGAGCCCACGGTGATAACACCAGTCAGACGATCAC
  ACCATGGAAGGGTCCAATGAGATCCAATGGAAAGACTTAGAACATCTAAGGTTGGGGGAG
  GTGCAACCTTCTGCTATTCAGCCCCTCTGCTCCAAGCGGAACCTTTTTTCTCAGGAGGTA
  ATCTCTAATAACAAGCAGAGCGCCCTCTGGAGCCTCCTCGCTGGTATCTCAGTGCCTGGA
  CAGAGGGGGACACACCACAGCACAAACACGTGGCACAGACTCAATCCCAACACACAGCCA
  GTCAACGAGCCTNTGCCCCCTTCCTCTGGGTCCTGAACAGAGCCTGCAGCGAGGGCTCTG
  AAGAGGTACAGGTAGCCCAGGCAGTGTTAGGTATAGCCACACCAGTCCCGGAGGACAGAG
  AAGCACCCTATAAACACTCGTGGCGCCCTGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000609
• Insert Size: 3520 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000609.3, NP_000600.1
• RefSeq Size: 3560 bp
• RefSeq ORF: 282 bp
• Locus ID: 6387
• Cytogenetics: 10q11.21
• Domains: IL8
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Leukocyte transendothelial migration
• Gene Summary: 'This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]'
  Transcript Variant: This variant (2) contains an alternate exon, and differs in the 3' coding region and UTR compared to variant 1. The resulting protein (isoform beta) is longer and has a distinct C-terminus compared to isoform alpha. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.