CD55 (NM_000574) Human Untagged Clone

CAT#: SC119802

CD55 (untagged)-Human CD55 molecule, decay accelerating factor for complement (Cromer blood group) (CD55), transcript variant 1


  "NM_000574" in other vectors (7)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CD55
Synonyms CHAPLE; CR; CROM; DAF; TC
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119802 sequence for NM_000574 edited (data generated by NextGen Sequencing)
ATGACCGTCGCGCGGCCGAGCGTGCCCGCGGCGCTGCCCCTCCTCGGGGAGCTGCCCCGG
CTGCTGCTGCTGGTGCTGTTGTGCCTGCCGGCCGTGTGGGGTGACTGTGGCCTTCCCCCA
GATGTACCTAATGCCCAGCCAGCTTTGGAAGGCCGTACAAGTTTTCCCGAGGATACTGTA
ATAACGTACAAATGTGAAGAAAGCTTTGTGAAAATTCCTGGCGAGAAGGACTCAGTGATC
TGCCTTAAGGGCAGTCAATGGTCAGATATTGAAGAGTTCTGCAATCGTAGCTGCGAGGTG
CCAACAAGGCTAAATTCTGCATCCCTCAAACAGCCTTATATCACTCAGAATTATTTTCCA
GTCGGTACTGTTGTGGAATATGAGTGCCGTCCAGGTTACAGAAGAGAACCTTCTCTATCA
CCAAAACTAACTTGCCTTCAGAATTTAAAATGGTCCACAGCAGTCGAATTTTGTAAAAAG
AAATCATGCCCTAATCCGGGAGAAATACGAAATGGTCAGATTGATGTACCAGGTGGCATA
TTATTTGGTGCAACCATCTCCTTCTCATGTAACACAGGGTACAAATTATTTGGCTCGACT
TCTAGTTTTTGTCTTATTTCAGGCAGCTCTGTCCAGTGGAGTGACCCGTTGCCAGAGTGC
AGAGAAATTTATTGTCCAGCACCACCACAAATTGACAATGGAATAATTCAAGGGGAACGT
GACCATTATGGATATAGACAGTCTGTAACGTATGCATGTAATAAAGGATTCACCATGATT
GGAGAGCACTCTATTTATTGTACTGTGAATAATGATGAAGGAGAGTGGAGTGGCCCACCA
CCTGAATGCAGAGGAAAATCTCTAACTTCCAAGGTCCCACCAACAGTTCAGAAACCTACC
ACAGTAAATGTTCCAACTACAGAAGTCTCACCAACTTCTCAGAAAACCACCACAAAAACC
ACCACACCAAATGCTCAAGCAACACGGAGTACACCTGTTTCCAGGACAACCAAGCATTTT
CATGAAACAACCCCAAATAAAGGAAGTGGAACCACTTCAGGTACTACCCGTCTTCTATCT
GGGCACACGTGTTTCACGTTGACAGGTTTGCTTGGGACGCTAGTAACCATGGGCTTGCTG
ACTTAG

Clone variation with respect to NM_000574.3
>OriGene 5' read for NM_000574 unedited
CGTCAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGGCGCGTC
CTTGTTCTAACCCGGCGCGCCATGACCGTCGCGCGGCCGAGCGTGCCCGCGGCGCTGCCC
CTCCTCGGGGAGCTGCCCCGGCTGCTGCTGCTGGTGCTGTTGTGCCTGCCGGCCGTGTGG
GGTGACTGTGGCCTTCCCCCANTGTACCTAATGCCCAGCCAGCTTTGGAAGGCCGTACAA
GTTTTCCCGAGGATACTGTAATAACGTACAAATGTGAAGAAAGCTTTGTGAAAATTCCTG
GCGAGAAGGACTCAGTGATCTGCCTTAAGGGCAGTCAATGGTCAGATATTGAAGAGTTCT
GCAATCGTAGCTGCGAGGTGCCAACAAGGCTAAATTCTGCATCCCTCAAACAGCCTTATA
TCACTCAGAATTATTTTCCAGTCGGTACTGTTGTGGAATATGAGTGCCGTCCAGGTTACA
GAAGAGAACCTTCTCTATCACCAAAACTAACTTGCCTTCAGAATTTAAAATGGTCCACAG
CAGTCGAATTTTGTAAAAAGAAATCATGCCCCTATCCGGGAGANATACGAAATGGTCAGA
TTGATGTACCAGGTGGCATATTATTTGGTGCCACCATCTCCTTCTCATGTAACACAGGNT
ACAAATTTATTGGGCTCGACTTCTAGTTTTTGTCTTATTTCAGGCAGCTCTGTCCAGTGG
AGTGACCCGTTTGCCAGAGTGCAGAGNAAATTTATTGTCCACCACACCCACAAATTGACC
ATGGNATAATTCAGGGGGGACGTGACCATTATGGATATAGACAGTCTGTAACGTATTGCC
ATGTATAAAGGATTCCCATTGATGGAAAGCCCTCCATTTAATTGACTGGGAATATGATGA
AAGAAATTGGAATGGCCCACCACCTGAATGCCAAGGAA
>OriGene 3' read for NM_000574 unedited
CTCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTACCCAAAAAAGGGACCTTTTTTTTTTTTTTAAGAAAAA
ACATTTTTTTTAACAAATTTTCTTAAACTTTTTTTGTTTTACATGAAAAACCAAAAGGGC
CCCTGGCTTAAATAAATTTAAATAAATTTTCCAAAAAAATAAAATTTCCAATTTTGCCTT
TGGCCCTCTCGATTAAAAAAAAAAAAAAAATCTTTTTTTATGTAAAAATGGGAATACCCC
GGGATCCTTTTAAAAACTTTAAGCCAAAAAAAACCCCTTTCAAAGGCTTTTTCCAAACTT
TTTTTGGGCAACAAAGGAAAATTCAGTCTTTCATTTTATTTTCCCCCCTTATAACCATAA
ACCCCCCGGGTTCACCAACATGTTTTACCTTTAAAATTTTTGGCCACCCCGAAGGAAAAA
TAAAAATGCCTTACCCCCAAAAATTCCTTTATAAGGGGGGCCATCTCTCTTTAACAATTT
TGCTTAAAAAAAACTGAATGGGGTTTTTATTTGCTTTGGAAAACCAAAAAAGGGTTTTCA
TTTTTTATTCCCTTTATAAGGGGAAATAACCTTCCAAATCTTGGGGAAAAAAAACCTTTC
CTTTTTTTTTCCCGGGGACCCCTTTTTAAATAAAAACAAATTCCTATGCTTTTTTACCCT
TTTAGGAAAGAGAACACCCTCATTTCTGCAAGTTGTTCTTTTTTCAAAAGGGGGAGGGGG
GCTAAAAAAGGGATCCCAGAACTGCCTTTTTTCCCCTGCCCTGGGGAACTTCCCAACCAC
ATCTTAAAGACATGAAACGCTTCCTAAAGAAAGAACCCAGTTGCTTTTTTTTTTCCAATT
GCCAAAAAGTTTAAAAAACTGGAAACAGCCGTATCTCTGGTGTTTCTTTTC
Restriction Sites NotI-NotI     
ACCN NM_000574
Insert Size 2310 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000574.2, NP_000565.1
RefSeq Size 2308 bp
RefSeq ORF 1146 bp
Locus ID 1604
Cytogenetics 1q32.2
Domains CCP
Protein Families Druggable Genome
Protein Pathways Complement and coagulation cascades, Hematopoietic cell lineage, Viral myocarditis
Gene Summary 'This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]'
Transcript Variant: This variant (1) encodes isoform 1 (also known as gDAF), which is membrane-associated. Sequence Note: This RefSeq record represents the CD55*001.1.1 allele.

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