Glutathione Peroxidase 1 (GPX1) (NM_000581) Human Untagged Clone

CAT#: SC119804

GPX1 (untagged)-Human glutathione peroxidase 1 (GPX1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPX1
• Synonyms: GPXD; GSHPX1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000581, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGTGCTGCTCGGCTAGCGGCGGCGGCGGCGGCGGCCCAGTCGGTGTATGCCTTCTCGGCGCGCCCGC
  TGGCCGGCGGGGAGCCTGTGAGCCTGGGCTCCCTGCGGGGCAAGGTACTACTTATCGAGAATGTGGCGTC
  CCTCTGAGGCACCACGGTCCGGGACTACACCCAGATGAACGAGCTGCAGCGGCGCCTCGGACCCCGGGGC
  CTGGTGGTGCTCGGCTTCCCGTGCAACCAGTTTGGGCATCAGGAGAACGCCAAGAACGAAGAGATTCTGA
  ATTCCCTCAAGTACGTCCGGCCTGGTGGTGGGTTCGAGCCCAACTTCATGCTCTTCGAGAAGTGCGAGGT
  GAACGGTGCGGGGGCGCACCCTCTCTTCGCCTTCCTGCGGGAGGCCCTGCCAGCTCCCAGCGACGACGCC
  ACCGCGCTTATGACCGACCCCAAGCTCATCACCTGGTCTCCGGTGTGTCGCAACGATGTTGCCTGGAACT
  TTGAGAAGTTCCTGGTGGGCCCTGACGGTGTGCCCCTACGCAGGTACAGCCGCCGCTTCCAGACCATTGA
  CATCGAGCCTGACATCGAAGCCCTGCTGTCTCAAGGGCCCAGCTGTGCCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000581 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCTGCTGGCCTC
  CCCTTATAGTGCTTGTTCGGGGCGCTCCGCTGGCTTCTTGGACAATTGCGCCATGTGTGC
  TGCTCGGCTAGCGGCGGCGGCGGCGGCCCAGTCGGTGTATGCCTTCTCGGCGCGCCCGCT
  GGCCGGCGGGGAGCCTGTGAGCCTGGGCTCCCTGCGGGGCAAGGTACTACTTATCGAGAA
  TGTGGCGTCCCTCTGAGGCACCACGGTCCGGGACTACACCCAGATGAACGAGCTGCAGCG
  GCGCCTCGGACCCCGGGGCCTGGTGGTGCTCGGCTTCCCGTGCAACCAGTTTGGGCATCA
  GGAGAACGCCAAGAACGAAGAGATTCTGAATTCCCTCAAGTACGTCCGGCCTGGTGGTGG
  GTTCCAGCCCAACTTCATGCTCTTCGAGAAGTGCGAGGTGAACGGTGCGGNGGCGCACCC
  TCTCTTCGCCTTCCTGCGGGAGGCCCTGCCAGCTCCCAGCGACGACGCCACCGCGCTTAT
  GACCGACCCCAAGCTCATCACCTGGTCTCCGGTGTGTCGCAACGATGTTGCCTGGAACTT
  TGAGAAGTTCCTGGTGGGCCCTGACGGTGTGCCCCTACGCAGGTACAGCCGCCGCTTCCA
  GACCATTGACATCGAGCCTGACATCGAAGCCCTGCTGTCTCAAGGGCTCAGCTGTGCCTA
  GGGCGCCCCTNCCTACCCNGCTGCTNTGCAGNTGCAGTGCTGCTGTCTCGGNNGGGTTTT
  CATCTATGAGGGGNTGTTNCCTCTAACCTACGAGGGNNAGACACCCTGATCTACAGAAAA
  TCCACCTCGAGATGGNTGCTGGTCCCTGTGATCCCAGTCTCTGCCAGACCAGGCGAGTTC
  NCCCACTATAAGGCGCCGGTTGTCAGCAGAAAAAAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000581 unedited
  TTTTTTTTTTTTTTTTTCGGGGGCACCCCCGGCACTTTATTAGGGGGGAAACTCCCCTTG
  GTCTGGCAAAAACTGGGATCAACAGGACCAGCCCCCATTTCGGAGGGGGTATTTTCTGTA
  AAAACAGGGGTTCCTCCCTCGTAGGTTTAAAGGAAACACCCTCATAAATGAAAACCCCCC
  CGAAACAGCAGCACTGCAACTGCCAAGCAGCCGGGGTAGGAGGGGCCCCCTAGGCACAGC
  TGACCCCTTGAAACAGCAGGGCTTCAATGTCAGGCTCGATGTCAATGGTCTGGAAGCGGC
  GGCTGTACCTGCGTAGGGGCACACCGTCAGGGCCCACCAGAAACTTTTAAAAGTTCCAGG
  CAACATCGTTGCGACACACCGGAAACCAGGTGATGAGCTTGGGGTCGGTCATAAGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000581
• Insert Size: 1000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000581.2, NP_000572.2
• RefSeq Size: 921 bp
• RefSeq ORF: 612 bp
• Locus ID: 2876
• Cytogenetics: 3p21.31
• Domains: GSHPx
• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Arachidonic acid metabolism, Glutathione metabolism, Huntington's disease
• Gene Summary: 'The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Other studies indicate that H2O2 is also essential for growth-factor mediated signal transduction, mitochondrial function, and maintenance of thiol redox-balance; therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is the most abundant, is ubiquitously expressed and localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. It is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. This gene contains an in-frame GCG trinucleotide repeat in the coding region, and three alleles with 4, 5 or 6 repeats have been found in the human population. The allele with 4 GCG repeats has been significantly associated with breast cancer risk in premenopausal women. Alternatively spliced transcript variants have been found for this gene. Pseudogenes of this locus have been identified on chromosomes X and 21. [provided by RefSeq, Aug 2017]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).