IL8 (CXCL8) (NM_000584) Human Untagged Clone

CAT#: SC119807

IL8 (untagged)-Human interleukin 8 (IL8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CXCL8
• Synonyms: GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1; SCYB8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000584 edited
  GAATTCGGCACGAGGCAGCAGAGCACACAAGCTTCTAGGACAAGAGCCAGGAAGAAACCA
  CCGGAAGGAACCATCTCACTGTGTGTAAACATGACTTCCAAGCTGGCCGTGGCTCTCTTG
  GCAGCCTTCCTGATTTCTGCAGCTCTGTGTGAAGGTGCAGTTTTGCCAAGGAGTGCTAAA
  GAACTTAGATGTCAGTGCATAAAGACATACTCCAAACCTTTCCACCCCAAATTTATCAAA
  GAACTGAGAGTGATTGAGAGTGGACCACACTGCGCCAACACAGAAATTATTGTAAAGCTT
  TCTGATGGAAGAGAGCTCTGTCTGGACCCCAAGGAAAACTGGGTGCAGAGGGTTGTGGAG
  AAGTTTTTGAAGAGGGCTGAGAATTCATAAAAAAATTCATTCTCTGTGGTATCCAAGAAT
  CAGTGAAGATGCCAGTGAAACTTCAAGCAAATCTACTTCAACACTTCATGTATTGTGTGG
  GTCTGTTGTAGGGTTGCCAGATGCAATACAAGATTCCTGGTTAAATTTGAATTTCAGTAA
  ACAATGAATAGTTTTTCATTGTACCATGAAATATCCAGAACATACTTATATGTAAAGTAT
  TATTTATTTGAATCTACAAAAAACAACAAATAATTTTTAAATATAAGGATTTTCCTAGAT
  ATTGCACGGGAGAATATACAAATAGCAAAATTGAGGCCAAGGGCCAAGAGAATATCCGAA
  CTTTAATTTCAGGAATTGAATGGGTTTGCTAGAATGTGATATTTGAAGCATCACATAAAA
  ATGATGGGACAATAAATTTTGCCATAAAGTCAAATTTAGCTGGAAATCCTGGATTTTTTT
  CTGTTAAATCTGGCAACCCTAGTCTGCTAGCCAGGATCCACAAGTCCTTGTTCCACTGTG
  CCTTGGTTTCTCCTTTATTTCTAAGTGGAAAAAGTATTAGCCACCATCTTACCTCACAGT
  GATGTTGTGAGGACATGTGGAAGCACTTTAAGTTTTTTCATCATAACATAAATTATTTTC
  AAGTGTAACTTATTAACCTATTTATTATTTATGTATTTATTTAAGCATCAAATATTTGTG
  CAAGAATTTGGAAAAATAGAAGATGAATCATTGATTGAATAGTTATAAAGATGTTATAGT
  AAATTTATTTTATTTTAGATATTAAATGATGTTTTATTAGATAAATTTCAATCAGGGTTT
  TTAGATTAAACAAACAAACAATTGGGTACCCAGTTAAATTTTCATTTCAGATATACAACA
  AATAATTTTTTAGTATAAGTACATTATTGTTTATCTGAAATTTTAATTGAACTAACAATC
  CTAGTTTGATACTCCCAGTCTTGTCATTGCCAGCTGTGTTGGTAGTGCTGTGTTGAATTA
  CGGAATAATGAGTTAGAACTATTAAAACAGCCAAAACTCCACAGTCAATATTAGTAATTT
  CTTGCTGGTTGAAACTTGTTTATTATGTACAAATAGATTCTTATAATATTATTTAAATGA
  CTGCATTTTTAAATACAAGGCTTTATATTTTTAACTTTAAGATGTTTTTATGTGCTCTCC
  AAATTTTTTTTACTGTTTCTGATTGTATGGAAATATAAAAGTAAATATGAAACATTTAAA
  ATATAATTTGTTGTCAAAGCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000584 unedited
  ACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCAGAGCACACAAGCTTCTAGGA
  CAAGAGCCAGGAAGAAACCACCGGAAGGAACCATCTCACTGTGTGTAAACATGACTTCCA
  AGCTGGCCGTGGCTCTCTTGGCAGCCTTCCTGATTTCTGCAGCTCTGTGTGAAGGTGCAG
  TTTTGCCAAGGAGTGCTAAAGAACTTAGATGTCAGTGCATAAAGACATACTCCAAACCTT
  TCCACCCCAAATTTATCAAAGAACTGAGAGTGATTGAGAGTGGACCACACTGCGCCAACA
  CAGAAATTATTGTAAAGCTTTCTGATGGAAGAGAGCTCTGTCTGGACCCCAAGGAAAACT
  GGGTGCAGAGGGTTGTGGAGAAGTTTTTGAAGAGGGCTGAGAATTCATAAAAAAATTCAT
  TCTCTGTGGTATCCAAGAATCAGTGAAGATGCCAGTGAAACTTCAAGCAAATCTACTTCA
  ACACTTCATGTATTGTGTGGGTCTGTTGTAGGGTTGCCAGATGCAATACAAGATTCCTGG
  TTAAATTTGAATTTCAGTAAACAATGAATAGTTTTTCATTGTACCATGAAATATCCAGAA
  CATACTTATATGTAAAGTATTATNTATTTGAATCTACAAAAAACACANNATATTTTTAAA
  TATAAGGATTTTCCTAGATATTGCACGGGGAGATATACAAATAGCANAATTGAGGCCCAG
  GGNCCAGAGGATATCCCGAACTTTATTNCAGGAANTGAATGGNTTGCTAGAATGTGATAT
  TTGAAGCATACATANAATGATGGGACANTAAATTTGCCATAAGTCAAATAGCTGGAAATC
  TGNATTNTTTTCTGTAAATCTGCACCCNTATCTGCTAGCCCAGATCCACAGTCCTTGTCC
  ACTGGGCCNTGGTNCTCCTTTATNCTAGNGGAAAAGTATANCCACCATCTACTNACATNG
  ATGTGGGAAGACATGTGAAGCTTNTAGTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000584 unedited
  ACCGCGGCCGCAATCNANAGTCGAGTTTTTTTTTTTTTTTTTTGCTTTGACAACAAATTA
  TATTTTAAATGTTTCATATTTACTTTTATATTTCCATACAATCAGAAACAGTAAAAAAAA
  TTTGGAGAGCACATAAAAACATCTTAAAGTTAAAAATATAAAGCCTTGTATTTAAAAATG
  CAGTCATTTAAATAATATTATAAGAATCTATTTGTACATAATAAACAAGTTTCAACCAGC
  AAGAAATTACTAATATTGACTGTGGAGTTTTGGCTGTTTTAATAGTTCTAACTCATTATT
  CCGTAATTCAACACAGCACTACCAACACAGCTGGCAATGACAAGACTGGGAGTATCAAAC
  TAGGATTGTTAGTTCAATTAAAATTTCAGATAAACAATAATGTACTTATACTAAAAAATT
  ATTTGTTGTATATCTGAAATGAAAATTTAACTGGGTACCCAATTGTTTGTTTGTTTAATC
  TAAAAACCCTGATTGAAATTTATCTAATAAAACATCATTTAATATCTAAAATAAAATAAA
  TTTACTATAACATCTTTATAACTATTCAATCAATGCATCATCTTCTATTTTTTCAAACTT
  CTTGCACAAATATTTGATGCCTAAATTAATCCCTAACTAATAAATTGGCTGATAAAGCTA
  CACCTTGAAAATAATTTATGTTATGGATGAAAAAACTTAAAGTGCTTACACCTGGGCTCA
  CCACATACCTGCGGAGGAAAAAGCGTGCCTAATACTTTTCCCCTTAGAAAAAAGGAAAAC
  CCGGGCTCATGGGAACAAGACCTGGGGTTCCTGGCTTACAACCCGGGTGGCCATATTACC
  GCACAACCCGGGATGCCAGCTTATTGGCCCTCGGACGAATTCTGCGCCCGTCTTTATGTG
  GGCCCGCGACCGCCTTCGCACGACCCTTGCCACCGTCTTTACTGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000584
• Insert Size: 1690 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000584.2, NP_000575.1
• RefSeq Size: 1666 bp
• RefSeq ORF: 300 bp
• Locus ID: 3576
• Cytogenetics: 4q13.3
• Domains: IL8
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Bladder cancer, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Epithelial cell signaling in Helicobacter pylori infection, NOD-like receptor signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is commonly referred to as interleukin-8 (IL-8). IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. Bacterial and viral products rapidly induce IL-8 expression. IL-8 also participates with other cytokines in the proinflammatory signaling cascade and plays a role in systemic inflammatory response syndrome (SIRS). This gene is believed to play a role in the pathogenesis of the lower respiratory tract infection bronchiolitis, a common respiratory tract disease caused by the respiratory syncytial virus (RSV). The overproduction of this proinflammatory protein is thought to cause the lung inflammation associated with csytic fibrosis. This proinflammatory protein is also suspected of playing a role in coronary artery disease and endothelial dysfunction. This protein is also secreted by tumor cells and promotes tumor migration, invasion, angiogenesis and metastasis. This chemokine is also a potent angiogenic factor. The binding of IL-8 to one of its receptors (IL-8RB/CXCR2) increases the permeability of blood vessels and increasing levels of IL-8 are positively correlated with increased severity of multiple disease outcomes (eg, sepsis). This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, May 2020]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).