IL8 (CXCL8) (NM_000584) Human Untagged Clone

CAT#: SC119807

IL8 (untagged)-Human interleukin 8 (IL8)


  "NM_000584" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CXCL8
Synonyms GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1; SCYB8
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_000584 edited
GAATTCGGCACGAGGCAGCAGAGCACACAAGCTTCTAGGACAAGAGCCAGGAAGAAACCA
CCGGAAGGAACCATCTCACTGTGTGTAAACATGACTTCCAAGCTGGCCGTGGCTCTCTTG
GCAGCCTTCCTGATTTCTGCAGCTCTGTGTGAAGGTGCAGTTTTGCCAAGGAGTGCTAAA
GAACTTAGATGTCAGTGCATAAAGACATACTCCAAACCTTTCCACCCCAAATTTATCAAA
GAACTGAGAGTGATTGAGAGTGGACCACACTGCGCCAACACAGAAATTATTGTAAAGCTT
TCTGATGGAAGAGAGCTCTGTCTGGACCCCAAGGAAAACTGGGTGCAGAGGGTTGTGGAG
AAGTTTTTGAAGAGGGCTGAGAATTCATAAAAAAATTCATTCTCTGTGGTATCCAAGAAT
CAGTGAAGATGCCAGTGAAACTTCAAGCAAATCTACTTCAACACTTCATGTATTGTGTGG
GTCTGTTGTAGGGTTGCCAGATGCAATACAAGATTCCTGGTTAAATTTGAATTTCAGTAA
ACAATGAATAGTTTTTCATTGTACCATGAAATATCCAGAACATACTTATATGTAAAGTAT
TATTTATTTGAATCTACAAAAAACAACAAATAATTTTTAAATATAAGGATTTTCCTAGAT
ATTGCACGGGAGAATATACAAATAGCAAAATTGAGGCCAAGGGCCAAGAGAATATCCGAA
CTTTAATTTCAGGAATTGAATGGGTTTGCTAGAATGTGATATTTGAAGCATCACATAAAA
ATGATGGGACAATAAATTTTGCCATAAAGTCAAATTTAGCTGGAAATCCTGGATTTTTTT
CTGTTAAATCTGGCAACCCTAGTCTGCTAGCCAGGATCCACAAGTCCTTGTTCCACTGTG
CCTTGGTTTCTCCTTTATTTCTAAGTGGAAAAAGTATTAGCCACCATCTTACCTCACAGT
GATGTTGTGAGGACATGTGGAAGCACTTTAAGTTTTTTCATCATAACATAAATTATTTTC
AAGTGTAACTTATTAACCTATTTATTATTTATGTATTTATTTAAGCATCAAATATTTGTG
CAAGAATTTGGAAAAATAGAAGATGAATCATTGATTGAATAGTTATAAAGATGTTATAGT
AAATTTATTTTATTTTAGATATTAAATGATGTTTTATTAGATAAATTTCAATCAGGGTTT
TTAGATTAAACAAACAAACAATTGGGTACCCAGTTAAATTTTCATTTCAGATATACAACA
AATAATTTTTTAGTATAAGTACATTATTGTTTATCTGAAATTTTAATTGAACTAACAATC
CTAGTTTGATACTCCCAGTCTTGTCATTGCCAGCTGTGTTGGTAGTGCTGTGTTGAATTA
CGGAATAATGAGTTAGAACTATTAAAACAGCCAAAACTCCACAGTCAATATTAGTAATTT
CTTGCTGGTTGAAACTTGTTTATTATGTACAAATAGATTCTTATAATATTATTTAAATGA
CTGCATTTTTAAATACAAGGCTTTATATTTTTAACTTTAAGATGTTTTTATGTGCTCTCC
AAATTTTTTTTACTGTTTCTGATTGTATGGAAATATAAAAGTAAATATGAAACATTTAAA
ATATAATTTGTTGTCAAAGCAAAAAAAAAAAAAAAAAACTCGAC
>OriGene 5' read for NM_000584 unedited
ACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGCAGAGCACACAAGCTTCTAGGA
CAAGAGCCAGGAAGAAACCACCGGAAGGAACCATCTCACTGTGTGTAAACATGACTTCCA
AGCTGGCCGTGGCTCTCTTGGCAGCCTTCCTGATTTCTGCAGCTCTGTGTGAAGGTGCAG
TTTTGCCAAGGAGTGCTAAAGAACTTAGATGTCAGTGCATAAAGACATACTCCAAACCTT
TCCACCCCAAATTTATCAAAGAACTGAGAGTGATTGAGAGTGGACCACACTGCGCCAACA
CAGAAATTATTGTAAAGCTTTCTGATGGAAGAGAGCTCTGTCTGGACCCCAAGGAAAACT
GGGTGCAGAGGGTTGTGGAGAAGTTTTTGAAGAGGGCTGAGAATTCATAAAAAAATTCAT
TCTCTGTGGTATCCAAGAATCAGTGAAGATGCCAGTGAAACTTCAAGCAAATCTACTTCA
ACACTTCATGTATTGTGTGGGTCTGTTGTAGGGTTGCCAGATGCAATACAAGATTCCTGG
TTAAATTTGAATTTCAGTAAACAATGAATAGTTTTTCATTGTACCATGAAATATCCAGAA
CATACTTATATGTAAAGTATTATNTATTTGAATCTACAAAAAACACANNATATTTTTAAA
TATAAGGATTTTCCTAGATATTGCACGGGGAGATATACAAATAGCANAATTGAGGCCCAG
GGNCCAGAGGATATCCCGAACTTTATTNCAGGAANTGAATGGNTTGCTAGAATGTGATAT
TTGAAGCATACATANAATGATGGGACANTAAATTTGCCATAAGTCAAATAGCTGGAAATC
TGNATTNTTTTCTGTAAATCTGCACCCNTATCTGCTAGCCCAGATCCACAGTCCTTGTCC
ACTGGGCCNTGGTNCTCCTTTATNCTAGNGGAAAAGTATANCCACCATCTACTNACATNG
ATGTGGGAAGACATGTGAAGCTTNTAGTTT
>OriGene 3' read for NM_000584 unedited
ACCGCGGCCGCAATCNANAGTCGAGTTTTTTTTTTTTTTTTTTGCTTTGACAACAAATTA
TATTTTAAATGTTTCATATTTACTTTTATATTTCCATACAATCAGAAACAGTAAAAAAAA
TTTGGAGAGCACATAAAAACATCTTAAAGTTAAAAATATAAAGCCTTGTATTTAAAAATG
CAGTCATTTAAATAATATTATAAGAATCTATTTGTACATAATAAACAAGTTTCAACCAGC
AAGAAATTACTAATATTGACTGTGGAGTTTTGGCTGTTTTAATAGTTCTAACTCATTATT
CCGTAATTCAACACAGCACTACCAACACAGCTGGCAATGACAAGACTGGGAGTATCAAAC
TAGGATTGTTAGTTCAATTAAAATTTCAGATAAACAATAATGTACTTATACTAAAAAATT
ATTTGTTGTATATCTGAAATGAAAATTTAACTGGGTACCCAATTGTTTGTTTGTTTAATC
TAAAAACCCTGATTGAAATTTATCTAATAAAACATCATTTAATATCTAAAATAAAATAAA
TTTACTATAACATCTTTATAACTATTCAATCAATGCATCATCTTCTATTTTTTCAAACTT
CTTGCACAAATATTTGATGCCTAAATTAATCCCTAACTAATAAATTGGCTGATAAAGCTA
CACCTTGAAAATAATTTATGTTATGGATGAAAAAACTTAAAGTGCTTACACCTGGGCTCA
CCACATACCTGCGGAGGAAAAAGCGTGCCTAATACTTTTCCCCTTAGAAAAAAGGAAAAC
CCGGGCTCATGGGAACAAGACCTGGGGTTCCTGGCTTACAACCCGGGTGGCCATATTACC
GCACAACCCGGGATGCCAGCTTATTGGCCCTCGGACGAATTCTGCGCCCGTCTTTATGTG
GGCCCGCGACCGCCTTCGCACGACCCTTGCCACCGTCTTTACTGCG
Restriction Sites NotI-NotI     
ACCN NM_000584
Insert Size 1690 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000584.2, NP_000575.1
RefSeq Size 1666 bp
RefSeq ORF 300 bp
Locus ID 3576
Cytogenetics 4q13.3
Domains IL8
Protein Families Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways Bladder cancer, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Epithelial cell signaling in Helicobacter pylori infection, NOD-like receptor signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway
Gene Summary 'The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is commonly referred to as interleukin-8 (IL-8). IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. Bacterial and viral products rapidly induce IL-8 expression. IL-8 also participates with other cytokines in the proinflammatory signaling cascade and plays a role in systemic inflammatory response syndrome (SIRS). This gene is believed to play a role in the pathogenesis of the lower respiratory tract infection bronchiolitis, a common respiratory tract disease caused by the respiratory syncytial virus (RSV). The overproduction of this proinflammatory protein is thought to cause the lung inflammation associated with csytic fibrosis. This proinflammatory protein is also suspected of playing a role in coronary artery disease and endothelial dysfunction. This protein is also secreted by tumor cells and promotes tumor migration, invasion, angiogenesis and metastasis. This chemokine is also a potent angiogenic factor. The binding of IL-8 to one of its receptors (IL-8RB/CXCR2) increases the permeability of blood vessels and increasing levels of IL-8 are positively correlated with increased severity of multiple disease outcomes (eg, sepsis). This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, May 2020]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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