PMS1 (NM_000534) Human Untagged Clone

CAT#: SC119824

PMS1 (untagged)-Human PMS1 postmeiotic segregation increased 1 (S. cerevisiae) (PMS1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PMS1
• Synonyms: HNPCC3; hPMS1; MLH2; PMSL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119824 sequence for NM_000534 edited (data generated by NextGen Sequencing)
  ATGAAACAATTGCCTGCGGCAACAGTTCGACTCCTTTCAAGTTCTCAGATCATCACTTCG
  GTGGTCAGTGTTGTAAAAGAGCTTATTGAAAACTCCTTGGATGCTGGTGCCACAAGCGTA
  GATGTTAAACTGGAGAACTATGGATTTGATAAAATTGAGGTGCGAGATAACGGGGAGGGT
  ATCAAGGCTGTTGATGCACCTGTAATGGCAATGAAGTACTACACCTCAAAAATAAATAGT
  CATGAAGATCTTGAAAATTTGACAACTTACGGTTTTCGTGGAGAAGCCTTGGGGTCAATT
  TGTTGTATAGCTGAGGTTTTAATTACAACAAGAACGGCTGCTGATAATTTTAGCACCCAG
  TATGTTTTAGATGGCAGTGGCCACATACTTTCTCAGAAACCTTCACATCTTGGTCAAGGT
  ACAACTGTAACTGCTTTAAGATTATTTAAGAATCTACCTGTAAGAAAGCAGTTTTACTCA
  ACTGCAAAAAAATGTAAAGATGAAATAAAAAAGATCCAAGATCTCCTCATGAGCTTTGGT
  ATCCTTAAACCTGACTTAAGGATTGTCTTTGTACATAACAAGGCAGTTATTTGGCAGAAA
  AGCAGAGTATCAGATCACAAGATGGCTCTCATGTCAGTTCTGGGGACTGCTGTTATGAAC
  AATATGGAATCCTTTCAGTACCACTCTGAAGAATCTCAGATTTATCTCAGTGGATTTCTT
  CCAAAGTGTGATGCAGACCACTCTTTCACTAGTCTTTCAACACCAGAAAGAAGTTTCATC
  TTCATAAACAGTCGACCAGTACATCAAAAAGATATCTTAAAGTTAATCCGACATCATTAC
  AATCTGAAATGCCTAAAGGAATCTACTCGTTTGTATCCTGTTTTCTTTCTGAAAATCGAT
  GTTCCTACAGCTGATGTTGATGTAAATTTAACACCAGATAAAAGCCAAGTATTATTACAA
  AATAAGGAATCTGTTTTAATTGCTCTTGAAAATCTGATGACGACTTGTTATGGACCATTA
  CCTAGTACAAATTCTTATGAAAATAATAAAACAGATGTTTCCGCAGCTGACATCGTTCTT
  AGTAAAACAGCAGAAACAGATGTGCTTTTTAATAAAGTGGAATCATCTGGAAAGAATTAT
  TCAAATGTTGATACTTCAGTCATTCCATTCCAAAATGATATGCATAATGATGAATCTGGA
  AAAAACACTGATGATTGTTTAAATCACCAGATAAGTATTGGTGACTTTGGTTATGGTCAT
  TGTAGTAGTGAAATTTCTAACATTGATAAAAACACTAAGAATGCATTTCAGGACATTTCA
  ATGAGTAATGTATCATGGGAGAACTCTCAGACGGAATATAGTAAAACTTGTTTTATAAGT
  TCCGTTAAGCACACCCAGTCAGAAAATGGCAATAAAGACCATATAGATGAGAGTGGGGAA
  AATGAGGAAGAAGCAGGTCTTGAAAACTCTTCGGAAATTTCTGCAGATGAGTGGAGCAGG
  GGAAATATACTTAAAAATTCAGTGGGAGAGAATATTGAACCTGTGAAAATTTTAGTGCCT
  GAAAAAAGTTTACCATGTAAAGTAAGTAATAATAATTATCCAATCCCTGAACAAATGAAT
  CTTAATGAAGATTCATGTAACAAAAAATCAAATGTAATAGATAATAAATCTGGAAAAGTT
  ACAGCTTATGATTTACTTAGCAATCGAGTAATCAAGAAACCCATGTCAGCAAGTGCTCTT
  TTTGTTCAAGATCATCGTCCTCAGTTTCTCATAGAAAATCCTAAGACTAGTTTAGAGGAT
  GCAACACTACAAATTGAAGAACTGTGGAAGACATTGAGTGAAGAGGAAAAACTGAAATAT
  GAAGAGAAGGCTACTAAAGACTTGGAACGATACAATAGTCAAATGAAGAGAGCCATTGAA
  CAGGAGTCACAAATGTCACTAAAAGATGGCAGAAAAAAGATAAAACCCACCAGCGCATGG
  AATTTGGCCCAGAAGCACAAGTTAAAAACCTCATTATCTAATCAACCAAAACTTGATGAA
  CTCCTTCAGTCCCAAATTGAAAAAAGAAGGAGTCAAAATATTAAAATGGTACAGATCCCC
  TTTTCTATGAAAAACTTAAAAATAAATTTTAAGAAACAAAACAAAGTTGACTTAGAAGAG
  AAGGATGAACCTTGCTTGATCCACAATCTCAGGTTTCCTGATGCATGGCTAATGACATCC
  AAAACAGAGGTAATGTTATTAAATCCATATAGAGTAGAAGAAGCCCTGCTATTTAAAAGA
  CTTCTTGAGAATCATAAACTTCCTGCAGAGCCACTGGAAAAGCCAATTATGTTAACAGAG
  AGTCTTTTTAATGGATCTCATTATTTAGACGTTTTATATAAAATGACAGCAGATGACCAA
  AGATACAGTGGATCAACTTACCTGTCTGATCCTCGTCTTACAGCGAATGGTTTCAAGATA
  AAATTGATACCAGGAGTTTCAATTACTGAAAATTACTTGGAAATAGAAGGAATGGCTAAT
  TGTCTCCCATTCTATGGAGTAGCAGATTTAAAAGAAATTCTTAATGCTATATTAAACAGA
  AATGCAAAGGAAGTTTATGAATGTAGACCTCGCAAAGTGATAAGTTATTTAGAGGGAGAA
  GCAGTGCGTCTATCCAGACAATTACCCATGTACTTATCAAAAGAGGACATCCAAGACATT
  ATCTACAGAATGAAGCACCAGTTTGGAAATGAAATTAAAGAGTGTGTTCATGGTCGCCCA
  TTTTTTCATCATTTAACCTATCTTCCAGAAACTACATGA
  
  Clone variation with respect to NM_000534.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000534 unedited
  GCAACGCCATTCGCACGAGGGCGCATCGGTTGAGACGCTGGCTGCTTGCGGCTAGTGGAT
  GGTAATTGCCTGCCTCGCGCTAGCAGGAAGCTGCTCTGTTAAAAGCGAAAATGAAACAAT
  TGCCTGCGGCAACAGTTCGACTCCTTTCAAGTTCTCAGATCATCACTTCGGTGGTCAGTG
  NTTAAAATAGCTTATTGAAAACTCCTTGGATGCTGGTGCCACAAGCGTAGATGTTAAACT
  GGAGAACTATGGATTTGATAAAATTGAGGTGCGAGATAACGGGGAGGGTATCAAGGCTGT
  TGATGCACCTGTAATGGCAATGAAGTACTACACCTCAAAAATAAATAGTCATGAAGATCT
  TGAAAATTTGACAACTTACGGTTTTCGTGGAGAAGCCTTGGGGTCAATTTGTTGTATAGC
  TGAGGTTTTAATTACAACAAGAACGGCTGCTGATAATTTTAGCACCCAGTATGTTTTAGA
  TGGCAGTGGCCACATACTTTCTCAGAAACCTTCACATCTTGGTCAAGGTACAACTGTAAC
  TGCTTTAAGATTATTTAAGAATCTACCTGTAAGAAAGCAGTTTTACTCAACTGCAAAAAA
  ATGTAAAGATGAAATAAAAAAGATCCAAGATCTCCTCATGAGCTTTGGTATCCTTAAACC
  TGACTTAAGGATTGTCTTTGTACATAACAAGGCAGTTATTTGGCAGAAAAGCAGAGTATC
  AGATCACAAGATGGCTCTCATGTCAGTTCTGGGGACTGCTGTTATGAACAATATGAAATC
  CTTTCAGTACCACTCTGAAGAATCTCAGATTTATCTCAGTGAATTTCTTCCAAGTGTGAT
  GCAGACCACTCTTTCCTAATCTTTCACACCAAAAAGATTTCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000534 unedited
  TGGACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAATAGTCTATATTA
  GTTTATTTACGTTTTCTACAATACGTGGAACTTTTTTCAATATAAAAACAAGTCAGTGAA
  TCCTCATTTAAAAAATAACCATGTGACACATAATACAAAGATAATTTAAAACCAGACTCA
  TGCTGTTTTATGACAGAACCAATTTCAATGGTAACTAATCTTCTTAAACATATTTAATCA
  TGTAGTTTCTGGAAGATAGGTTAAATGATGAAAAAATGGGCGACCATGAACACACTCTTT
  AATTTCATTTCCAAACTGGTGCTTCATTCTGTAGATAATGTCTTGGATGTCCTCTTTTGA
  TAAGTACATGGGTAATTGTCTGGATAGACGCACTGCTTCTCCCTCTAAATAACTTATCAC
  TTTGCGAGGTCTACATTCATAAACTTCCTTTGCATTTCTGTTTAATATAGCATTAAGAAT
  TTCTTTTAAATCTGCTACTCCATAGAATGGGAGACAATTAGCCATTCCTTCTATTTCCAA
  GTAATTTTCAGTAATTGAAACTCCTGGTATCAATTTTATCTTGAAACCATTCGCTGTAAG
  ACGAGGATCAGACAGGTAAGTTGATCCACTGTATCTTTGGTCATCTGCTGTCATTTTATA
  TAAAACGTCTAAATAATGAGATCCATTAAAAAGACTCTCTGTTAACATAATTGGCTTTTC
  CAGTGGCTCTGCANGAAAGTTATGATTCTCAAGAAAGTCTTTAATAGCANGGCTTCTTCT
  ACTCATATGGATTTATAAACATACCTCTGTTTGGNATGTCATTAGCCATGCATCAAGAAC
  CTGAGATGTGGATCAAGCGGTTCATCCTTCTCTCTAGNCACTTGGTTTGTTCTTAAATTA
  TTTTTAGTTTTATAGAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000534
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000534.3, NP_000525.1
• RefSeq Size: 3239 bp
• RefSeq ORF: 2799 bp
• Locus ID: 5378
• Cytogenetics: 2q32.2
• Domains: HMG, DNA_mis_repair, HATPase_c
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a protein belonging to the DNA mismatch repair mutL/hexB family. This protein is thought to be involved in the repair of DNA mismatches, and it can form heterodimers with MLH1, a known DNA mismatch repair protein. Mutations in this gene cause hereditary nonpolyposis colorectal cancer type 3 (HNPCC3) either alone or in combination with mutations in other genes involved in the HNPCC phenotype, which is also known as Lynch syndrome. [provided by RefSeq, Jul 2008]'