Acid sphingomyelinase (SMPD1) (NM_000543) Human Untagged Clone

CAT#: SC119827

SMPD1 (untagged)-Human sphingomyelin phosphodiesterase 1, acid lysosomal (SMPD1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMPD1
• Synonyms: ASM; ASMASE; NPD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119827 sequence for NM_000543 edited (data generated by NextGen Sequencing)
  ATGCCCCGCTACGGAGCGTCACTCCGCCAGAGCTGCCCCAGGTCCGGCCGGGAGCAGGGA
  CAAGACGGGACCGCCGGAGCCCCCGGACTCCTTTGGATGGGCCTGGCGCTGGCGCTGGCG
  CTGGCGCTGGCGCTGGCGCTGGCTCTGTCTGACTCTCGGGTTCTCTGGGCTCCGGCAGAG
  GCTCACCCTCTTTCTCCCCAAGGCCATCCTGCCAGGTTACATCGCATAGTGCCCCGGCTC
  CGAGATGTCTTTGGGTGGGGGAACCTCACCTGCCCAATCTGCAAAGGTCTATTCACCGCC
  ATCAACCTCGGGCTGAAGAAGGAACCCAATGTGGCTCGCGTGGGCTCCGTGGCCATCAAG
  CTGTGCAATCTGCTGAAGATAGCACCACCTGCCGTGTGCCAATCCATTGTCCACCTCTTT
  GAGGATGACATGGTGGAGGTGTGGAGACGCTCAGTGCTGAGCCCATCTGAGGCCTGTGGC
  CTGCTCCTGGGCTCCACCTGTGGGCACTGGGACATTTTCTCATCTTGGAACATCTCTTTG
  CCTACTGTGCCGAAGCCGCCCCCCAAACCCCCTAGCCCCCCAGCCCCAGGTGCCCCTGTC
  AGCCGCATCCTCTTCCTCACTGACCTGCACTGGGATCATGACTACCTGGAGGGCACGGAC
  CCTGACTGTGCAGACCCACTGTGCTGCCGCCGGGGTTCTGGCCTGCCGCCCGCATCCCGG
  CCAGGTGCCGGATACTGGGGCGAATACAGCAAGTGTGACCTGCCCCTGAGGACCCTGGAG
  AGCCTGTTGAGTGGGCTGGGCCCAGCCGGCCCTTTTGATATGGTGTACTGGACAGGAGAC
  ATCCCCGCACATGATGTCTGGCACCAGACTCGTCAGGACCAACTGCGGGCCCTGACCACC
  GTCACAGCACTTGTGAGGAAGTTCCTGGGGCCAGTGCCAGTGTACCCTGCTGTGGGTAAC
  CATGAAAGCACACCTGTCAATAGCTTCCCTCCCCCCTTCATTGAGGGCAACCACTCCTCC
  CGCTGGCTCTATGAAGCGATGGCCAAGGCTTGGGAGCCCTGGCTGCCTGCCGAAGCCCTG
  CGCACCCTCAGAATTGGGGGGTTCTATGCTCTTTCCCCATACCCCGGTCTCCGCCTCATC
  TCTCTCAATATGAATTTTTGTTCCCGTGAGAACTTCTGGCTCTTGATCAACTCCACGGAT
  CCCGCAGGACAGCTCCAGTGGCTGGTGGGGGAGCTTCAGGCTGCTGAGGATCGAGGAGAC
  AAAGTGCATATAATTGGCCACATTCCCCCAGGGCACTGTCTGAAGAGCTGGAGCTGGAAT
  TATTACCGAATTGTAGCCAGGTATGAGAACACCCTGGCTGCTCAGTTCTTTGGCCACACT
  CATGTGGATGAATTTGAGGTCTTCTATGATGAAGAGACTCTGAGCCGGCCGCTGGCTGTA
  GCCTTCCTGGCACCCAGTGCAACTACCTACATCGGCCTTAATCCTGGTTACCGTGTGTAC
  CAAATAGATGGAAACTACTCCGGGAGCTCTCACGTGGTCCTGGACCATGAGACCTACATC
  CTGAATCTGACCCAGGCAAACATACCGGGAGCCATACCGCACTGGCAGCTTCTCTACAGG
  GCTCGAGAAACCTATGGGCTGCCCAACACACTGCCTACCGCCTGGCACAACCTGGTATAT
  CGCATGCGGGGCGACATGCAACTTTTCCAGACCTTCTGGTTTCTCTACCATAAGGGCCAC
  CCACCCTCGGAGCCCTGTGGCACGCCCTGCCGTCTGGCTACTCTTTGTGCCCAGCTCTCT
  GCCCGTGCTGACAGCCCTGCTCTGTGCCGCCACCTGATGCCAGATGGGAGCCTCCCAGAG
  GCCCAGAGCCTGTGGCCAAGGCCACTGTTTTGCTAG
  
  Clone variation with respect to NM_000543.4
  107 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000543 unedited
  GTTGACTATTTTGTAATACGACTTTACTATAGGGCGGCCGCGAATTCGCACGAGGGGACA
  GAGAGAGGAAGAGGAAGGGGCGGAGCTGCTTTGCGGCCGGCCGCGGAGCAGTCAGCCGAC
  TACAGAGAAGGGTAATCGGGTGTCCCCGGCGCCGCCCGGGGCCCTGAGGGCTGGCTAGGG
  TCCAGGCCGGGGGGGACGGGACAGACGAACCAGCCCCGTGTAGGAAGCGCGACAATGCCC
  CGCTACGGAGCGTCACTCCGCCAGAGCTGCCCCAGGTCCGGCCGGGAGCAGGGACAAGAC
  GGGACCGCCGGAGCCCCCGGACTCCTTTGGATGGGCCTGGCGCTGGCGCTGGCGCTGGCG
  CTGGCGCTGGCGCTGGCTCTGTCTGACTCTCGGGTTCTCTGGGCTCCGGCAGAGGCTCAC
  CCTCTTTCTCCCCAAGGCCATCCTGCCAGGTTACATCGCATAGTGCCCCGGCTCCGAGAT
  GTCTTTGGGTGGGGGAACCTCACCTGCCCAATCTGCAAAGGTCTATTCACCGCCATCAAC
  CTCGGGCTGAAGAAGGAACCCAATGTGGCTCGCGTGGGCTCCGTGGCCATCAAGCTGTGC
  AATCTGCTGAAGATAGCACCACCTGCCGTGTGCCAATCCATTGTCCACCTCTTTGAGGAT
  GACATGGTGGAGGTGTGGAGACGCTCAGTGCTGAGCCCATCTGAGGCCTGTGGCCTGCTC
  CTGGGCTCCACCTGTGGGCACTGGGACATTTTCTCATCTTGGAACATCTCTTTGCCTACT
  GGCCGAAAACCCGCCCCCCCAAACCCCCTAGCCCCCCAACCCCAAGTGGCCCCTGGCACC
  CGCATCCTCTTCTCACTGACCTGCACTGGGATCATGACTACCTGGGAGGCCACGGACCCT
  GATTGTGCAAACACACTGTTGTTGCCCCCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000543 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGAGTCCAAGTCTCTTATCTTTA
  TTTTAACAGACTGGCAGCATCAGGTCGCAGCAGCAGTACAGGGTTCCTGGCTGGGCAGCA
  CAGGCCTGGGGCGACAGCTCCTGTCTTGTCTGCCCAGGGCAGTGTCAACTTAAGCCTCTA
  CTCCATGGCTGTGAAAGGACAGCAGCCTCAAGGCAGTCTAGCTCCTGGGCACAGGCAGCC
  AAACCCCCTCCCATATCCAGCTAAACCAGCTCCAGGAAAGGAGAAGGTCCTGTTTCCCCG
  GCATCCTTGGGGCCCAGGGACTGGTTCTTTCACCGGATGATCTTGCCTGGTTGAACCACA
  GCAGCATTTGGGCTTTTTCACCCTTTCCTACATCAAGAACTTTCCCAAATGTGGGCCCTG
  GGGCCCTAGCAAAACAGTGGCCTTGGCCACAGGCTCTGGGCCTCTGGGAGGCTCCCATCT
  GGCATCAGGTGGCGGCACAGAGCAGGGCTGTCAGCACGGGCAGAGAGCTGGGCACAAAGA
  GTAGCCAGACGGCAGGGCGTGCCACAGGGCTCCGAGGGTGGGTGGCCCTTATGGTAGAGA
  AACCAGAAGGTCTGGAAAAGTTGCATGTCGCCCCGCATGCGATATACCATGTTGTGCCAG
  GCGGTATGCAGTGTGTTGGGCAGCCCATATGTTTCTCGAGCCCTGTAGAGAATCTGNCAG
  TGCGGTATGGCTCCCCGTATGTTTGCCTGGGTCAAATTCANGATGTAGGTCTCATGGTCC
  CAGAACACGTGAGATGCTCCGGAGTAGTTTCCATCTATTTGGTACACACGGTACCCAGAT
  TAAGCCGATGTAAGTAGTTGCACTGGGTGCCANGAAAGCTACAGCCANNCGCCGGCTCAA
  AGTCTCTTCTCATAGAAGACCTCCAATCCTCCACCATGATGTTGGCCAAGAACTGAGCGC
  NCAGGGGTGGTCTCATACCTGGCTACANTNCNGNNNATANTCCACNTCCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000543
• Insert Size: 2640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000543.3, NP_000534.3
• RefSeq Size: 2473 bp
• RefSeq ORF: 1896 bp
• Locus ID: 6609
• Cytogenetics: 11p15.4
• Domains: Metallophos, SAPB
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome, Metabolic pathways, Sphingolipid metabolism
• Gene Summary: 'The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).