GALNS (NM_000512) Human Untagged Clone

CAT#: SC119830

GALNS (untagged)-Human galactosamine (N-acetyl)-6-sulfate sulfatase (GALNS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GALNS
• Synonyms: GalN6S; GALNAC6S; GAS; MPS4A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119830 sequence for NM_000512 edited (data generated by NextGen Sequencing)
  ATGGCGGCGGTTGTCGCGGCGACGAGGTGGTGGCAGCTGTTGCTGGTGCTCAGCGCCGCG
  GGGATGGGGGCCTCGGGCGCCCCGCAGCCCCCCAACATCCTGCTCCTGCTCATGGACGAC
  ATGGGATGGGGTGACCTCGGGGTGTATGGAGAGCCCTCCAGAGAGACCCCGAATTTGGAC
  CGGATGGCTGCAGAAGGGCTGCTTTTCCCAAACTTCTATTCTGCCAACCCTCTGTGCTCG
  CCATCGAGGGCGGCACTGCTCACAGGACGGCTACCCATCCGCAATGGCTTCTACACCACC
  AACGCCCATGCCAGAAACGCCTACACACCGCAGGAGATTGTGGGCGGCATCCCAGACTCG
  GAGCAGCTCCTGCCGGAGCTTCTGAAGAAGGCCGGCTACGTCAGCAAGATTGTCGGCAAG
  TGGCATCTGGGTCACAGGCCCCAGTTCCACCCCCTGAAGCACGGATTTGATGAGTGGTTT
  GGATCCCCCAACTGCCACTTTGGACCTTATGACAACAAGGCCAGGCCCAACATCCCTGTG
  TACAGGGACTGGGAGATGGTTGGCAGATATTATGAAGAATTTCCTATTAATCTGAAGACG
  GGGGAAGCCAACCTCACCCAGATCTACCTGCAGGAAGCCCTGGACTTCATTAAGAGACAG
  GCACGGCACCACCCCTTTTTCCTCTACTGGGCTGTCGACGCCACGCACGCACCCGTCTAT
  GCCTCCAAACCCTTCTTGGGCACCAGTCAGCGAGGGCGGTATGGAGACGCCGTCCGGGAG
  ATTGATGACAGCATTGGGAAGATACTGGAGCTCCTCCAAGACCTGCACGTCGCGGACAAC
  ACCTTCGTCTTCTTCACGTCGGACAACGGCGCTGCCCTCATTTCCGCCCCCGAACAAGGT
  GGCAGCAACGGCCCCTTTCTGTGTGGGAAGCAGACCACGTTTGAAGGAGGGATGAGGGAG
  CCTGCCCTCGCATGGTGGCCAGGGCACGTCACTGCAGGCCAGGTGAGCCACCAGCTGGGC
  AGCATCATGGACCTCTTCACCACCAGCCTGGCCCTTGCGGGCCTGACGCCGCCCAGCGAC
  AGGGCCATTGATGGCCTCAACCTCCTCCCCACCCTCCTGCAGGGCCGGCTGATGGACAGG
  CCTATCTTCTATTACCGTGGCGACACGCTGATGGCGGCCACCCTCGGGCAGCACAAGGCT
  CACTTCTGGACCTGGACCAACTCCTGGGAGAACTTCAGACAGGGCATTGATTTCTGCCCT
  GGGCAGAACGTTTCAGGGGTCACAACTCACAATCTGGAAGACCACACGAAGCTGCCCCTG
  ATCTTCCACCTGGGACGGGACCCAGGGGAGAGGTTCCCCCTCAGCTTTGCCAGCGCCGAG
  TACCAGGAGGCCCTCAGCAGGATCACCTCGGTCGTCCAGCAGCACCAGGAGGCCTTGGTC
  CCCGCGCAGCCCCAGCTCAACGTGTGCAACTGGGCGGTCATGAACTGGGCACCTCCGGGC
  TGTGAAAAGTTAGGGAAGTGTCTGACACCTCCAGAATCCATTCCCAAGAAGTGCCTCTGG
  TCCCACTAG
  
  Clone variation with respect to NM_000512.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000512 unedited
  CACATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGGGCCGGAGG
  GCCGGCGGACGCTCGCTAGGTCGGCTCGCTGGCCGGGGCTCCGCGGCTCCCGTGGTTGCC
  ATGGCGGCGGTTGTCGCGGCGACGAGGTGGTGGCAGCTGTTGCTGGTGCTCAGCGCCGCG
  GGGATGGGGGCCTCGGGCGCCCCGCAGCCCCCCAACATCCTGCTCCTGCTCATGGACGAC
  ATGGGATGGGGTGACCTCGGGGTGTATGGAGAGCCCTCCAGAGAGACCCCGAATTTGGAC
  CGGATGGCTGCAGAAGGGCTGCTTTTCCCAAACTTCTATTCTGCCAACCCTCTGTGCTCG
  CCATCGAGGGCGGCACTGCTCACAGGACGGCTACCCATCCGCAATGGCTTCTACACCACC
  AACGCCCATGCCAGAAACGCCTACACACCGCAGGAGATTGTGGGCGGCATCCCAGACTCG
  GAGCAGCTCCTGCCGGAGCTTCTGAAGAAGGCCGGCTACGTCAGCAAGATTGTCGGCAAG
  TGGCATCTGGGTCACAGGCCCCAGTTCCACCCCCTGAAGCACGGATTTGATGAGTGGTTT
  GGATCCCCCAACTGCCACTTTGGACCTTATGACAACAAGGCCAGGCCCAACATCCCTGTG
  TACAGGGACTGNGAGATGGTTGGCAGATATTATGAAGAATTTCCTATTTAATCTGAGACG
  GGGNAAGCCNACCTCACCCAGATCTACCCTGCAGAAGCCCTGGACTTCATTTAGAGACAG
  GCACGGCACCACCCCCTTTTTCCTCTACTGGGCTGTCGACGCCACGCACGCACCCGTCTT
  TGCCTCCAAACCTTCTTGGGCACAGTCANCGAGGGCGGTTTGGAAACCCCGTTCCCGGAG
  ATTGATGAAGCATGNGGGAGGAACTGGGACTCCTCCAGACCTGCACCGTGCGGGCACCCC
  T
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000512 unedited
  TCCTGCCTCCCGCTTNTATTTCTAAATAACATAGTTACAATGATAAGAAGCTACACGCCT
  CCCTCATAATTANGCGTCCAGGNGAAATTCCTTGTGGACAAAGGACAGACGGAACTGGAA
  GTCATCCCTCTGGGGCTCCCCTGAGACAAACGCTTATATGATTGCCCCGTTCCCTTACTG
  TTTACATAAAAATGCGGATTCACTGGGCCAGACTCAATCGTGTATTCAGTGGAAGGCTGA
  CTGAACCAATGTACGCCATACACATACTGATCTTGTGTCTCCCTAAGATGTATAAAGGCA
  AACTGTATCCCCAGCCACCTCAGGCACCTGTTGTCAGGACCTCCTGAGGCTGTCACAGGT
  GCATCCTTAACCTTGGCAAAATAAACTGAGACTGGTCTCAGATATTTGGGGTTCACAAAG
  GCGTGAGACGGCAGGGTCCTGAGGTCTGAGGCGCCGTGGGCGAGGAGGAGGGTCCTGAAA
  TCTGAGGCGCCGTGGGCGAGGAGGAGGGCCAAGCACACGCCAGGGTCAGGTCCTGGGCAG
  GTGGAATTGTGCAGTCCCCCTGCGTCTGCAGGTGCTGTCTGTCTGGCTTGGGCAGGGTTG
  GGGGAGGACCGAGGCCAGAGCCATCCTTCCTCCAGGCACTTGCAGGGCCAACCGGAGATT
  CTAGGCCTGGCCTGAGTCTGCGCAGGTGCTAGTGGGACCAGAGGCACTTCTTGGGAATGG
  ATCCNNTGAGTGTCAGACACTTCCCTAACTTTTCACAGCCCCGGAGGTGCCCAGTTCATG
  ACCGNCCAGTTGCACACGTTTGAGCTGGGGCTGCCGCGGGACCAAAGCCTCCTGGTGCTG
  CTGGACGACCGAAGTGATCCTGCTGGAGGCCTCCTGGTACTCGGCGCTGGCAAGCTGAGG
  GGGAACTCTCCCCTGGTCCCGTCCAGGTGGAGATCAGGCCANTTCGGTGGCTTTAGATTG
  GAGTGGACCCTGAACTTCTGCAGGCAGAATAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000512
• Insert Size: 2640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000512.3, NP_000503.1
• RefSeq Size: 2390 bp
• RefSeq ORF: 1569 bp
• Locus ID: 2588
• Cytogenetics: 16q24.3
• Domains: Sulfatase
• Protein Families: Druggable Genome
• Protein Pathways: Glycosaminoglycan degradation, Lysosome, Metabolic pathways
• Gene Summary: 'This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq, Jul 2008]'