p53 (TP53) (NM_000546) Human Untagged Clone

CAT#: SC119832

TP53 (untagged)-Human tumor protein p53 (TP53), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TP53
• Synonyms: BCC7; BMFS5; LFS1; P53; TRP53
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000546 edited
  ATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGCCCCCTCTGAGTCAGGAAACATTTTCA
  GACCTATGGAAACTACTTCCTGAAAACAACGTTCTGTCCCCCTTGCCGTCCCAAGCAATG
  GATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGAAGACCCAGGTCCA
  GATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGTGGCCCCTGCACCAGCAGCTCCT
  ACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAG
  AAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCTGGGACAGCCAAG
  TCTGTGACTTGCACGTACTCCCCTGCCCTCAACAAGATGTTTTGCCAACTGGCCAAGACC
  TGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGCGTCCGCGCCATG
  GCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCCCCCACCATGAG
  CGCTGCTCAGATAGCGATGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGGAAAT
  TTGCGTGTGGAGTATTTGGATGACAGAAACACTTTTCGACATAGTGTGGTGGTGCCCTAT
  GAGCCGCCTGAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGT
  TCCTGCATGGGCGGCATGAACCGGAGGCCCATCCTCACCATCATCACACTGGAAGACTCC
  AGTGGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCTGTCCTGGGAGA
  GACCGGCGCACAGAGGAAGAGAATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCC
  CCAGGGAGCACTAAGCGAGCACTGCCCAACAACACCAGCTCCTCTCCCCAGCCAAAGAAG
  AAACCACTGGATGGAGAATATTTCACCCTTCAGATCCGTGGGCGTGAGCGCTTCGAGATG
  TTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATGCCCAGGCTGGGAAGGAGCCAGGG
  GGGAGCAGGGCTCACTCCAGCCACCTGAAGTCCAAAAAGGGTCAGTCTACCTCCCGCCAT
  AAAAAACTCATGTTCAAGACAGAAGGGCCTGACTCAGACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000546 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTTGCGTTCGGGC
  TGGGAGCGTGCTTTCCACGACGGTGACACGCTTCCCTGGATTGGCAGCCAGACTGCCTTC
  CGGGTCACTGCCATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGCCCCCTCTGAGTCAG
  GAAACATTTTCAGACCTATGGAAACTACTTCCTGAAAACAACGTTCTGTCCCCCTTGCCG
  TCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGAA
  GACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGTGGCCCCTGCA
  CCAGCAGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCATCTTCT
  GTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCT
  GGGACAGCCAAGTCTGTGACTTGCACGTACTCCCCTGCCCTCAACAAGATGTTTTGCCAA
  CTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCGCCCGGCACCCGC
  GTCCGCGCCATGGCCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTG
  CCCCCACCATGAGCGCTGCTCAGATAGCGATGGTCTGCCCCTCCTCAGCATCTTATCCGA
  GTGGAAAGAAATTTGGCGTGTGGAGTATTTGGATGACAGAAAACACTTTTCCACATATGG
  GNGTGGTCGCCCTAAGAGCCCCCCTGAGGTTGGGTCTGACTGTCCCACCATCCCTTCCAC
  TAACTGGGAAACAGTTCTTGCTTGGCCGGGATGAACCGGAAGGCCCTCCTTACCTCATCA
  CACTGGAAGACTCCGGGGAATTCACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000546 unedited
  CCGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGCAGCAAAGTTTTATTG
  TAAAATAAGAGATCGATATAAAAATGGGATATAAAAAGGGAGAAGGAGGGGAAGGGTGGG
  GTGAAAATGCAGATGTGCTTGCAGAATGTAAAAGATGTTGACCCTTCCAGCTGGACGTGG
  TGGCTCACAATTGTAATCCCAGCACTCTGGGAGGCTGAGACAGGTGGATCGCCTGAGCCC
  AGGAGTTTGAGACCAGCCTGGGCAACACTGTGAGACCCCATCTCTACAAAACATGCAAAA
  GTTGGCTGGCCATGGTGGCATGAACCTGTGGTCCCAGCTACTCCGGAGGCTGAGGCAGGA
  CTGCTCGAGCCGGGGAGGCAAAGGCTGCAGTAAGCCAAGATCACGCCACTCCACTCCAGT
  CTGGGCAACTAAGCGAGACCCAGTCTTCAAGTAATTAGACCCCTTTCATTCCACTCCAAA
  TCCGATTTTGACCCTGAGCCTATAACAAGTCTTCGGTGGATCCCGCACCATCCTATACAA
  AAACATGAAATTCTTCAAGGGTGTGGGATGCGGGTGACAATTTCCCTTTTACGGTTCCAA
  CGGTTACCCAACAGGTTGGCCCACAATGGTTTGTGCTGGGCCCCTCATAACACTTGATAA
  CTCCCCTCTTACTTAACCACGTTTCACAAATACGTATATACTCCTCCCACCCCTCTTCAC
  ACCATCGGCTATGGGCACCCCCCCTCCGTCAAGGCGCGTCTACTCCCTCCCCCCACCTCT
  CACATAACAGTCTCTACACCCGAGTCTCACTCCTCTTCTATTTATTTCCTCCTCCACCCC
  GCTCTCCTGCCCGTCTGCTTCCCCATTCTACCCCTCCCCTCCCTCAATTATTACCCCTCT
  CTCTCACTCTATAACTCCCAAAGTGCCATGCCACACATTTATCTTCTCCCGTTCTACCCG
  CCACAGTTCACTCTCTCCGTATAATCGCATTCTAAACATCCGTCGCTCACCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000546
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000546.2, NP_000537.2
• RefSeq Size: 2629 bp
• RefSeq ORF: 1182 bp
• Locus ID: 7157
• Cytogenetics: 17p13.1
• Domains: P53
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway
• Gene Summary: 'This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]'
  Transcript Variant: This variant (1) can initiate translation from two in-frame AUG start codons. The isoform represented in this variant (a, also known as p53alpha) results from translation initiation at the upstream start codon. Both variants 1 and 2 encode isoform a, which is the longest isoform.