Presenilin 2 (PSEN2) (NM_000447) Human Untagged Clone

CAT#: SC119867

PSEN2 (untagged)-Human presenilin 2 (Alzheimer disease 4) (PSEN2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSEN2
• Synonyms: AD3L; AD4; CMD1V; PS2; STM2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000447 edited
  CAGAGCAGGCATTTCCAGCAGTGAGGAGACAGCCAGAAGCAAGCTTTTGGAGCTGAAGGA
  ACCTGAGACAGAAGCTAGTCCCCCCTCTGAATTTTACTGATGAAGAAACTGAGGCCACAG
  AGCTAAAGTGACTTTTCCCAAGGTCGCCCAGCGAGGACGTGGGACTTCTCAGACGTCAGG
  AGAGTGATGTGAGGGAGCTGTGTGACCATAGAAAGTGACGTGTTAAAAACCAGCGCTGCC
  CTCTTTGAAAGCCAGGGAGCATCATTCATTTAGCCTGCTGAGAAGAAGAAACCAAGTGTC
  CGGGATTCAGACCTCTCTGCGGCCCCAAGTGTTCGTGGTGCTTCCAGAGGCAGGGCTATG
  CTCACATTCATGGCCTCTGACAGCGAGGAAGAAGTGTGTGATGAGCGGACGTCCCTAATG
  TCGGCCGAGAGCCCCACGCCGCGCTCCTGCCAGGAGGGCAGGCAGGGCCCAGAGGATGGA
  GAGAATACTGCCCAGTGGAGAAGCCAGGAGAACGAGGAGGACGGTGAGGAGGACCCTGAC
  CGCTATGTCTGTAGTGGGGTTCCCGGGCGGCCGCCAGGCCTGGAGGAAGAGCTGACCCTC
  AAATACGGAGCGAAGCATGTGATCATGCTGTTTGTGCCTGTCACTCTGTGCATGATCGTG
  GTGGTAGCCACCATCAAGTCTGTGCGCTTCTACACAGAGAAGAATGGACAGCTCATCTAC
  ACGCCATTCACTGAGGACACACCCTCGGTGGGCCAGCGCCTCCTCAACTCCGTGCTGAAC
  ACCCTCATCATGATCAGCGTCATCGTGGTTATGACCATCTTCTTGGTGGTGCTCTACAAG
  TACCGCTGCTACAAGTTCATCCATGGCTGGTTGATCATGTCTTCACTGATGCTGCTGTTC
  CTCTTCACCTATATCTACCTTGGGGAAGTGCTCAAGACCTACAATGTGGCCATGGACTAC
  CCCACCCTCTTGCTGACTGTCTGGAACTTCGGGGCAGTGGGCATGGTGTGCATCCACTGG
  AAGGGCCCTCTGGTGCTGCAGCAGGCCTACCTCATCATGATCAGTGCGCTCATGGCCCTA
  GTGTTCATCAAGTACCTCCCAGAGTGGTCCGCGTGGGTCATCCTGGGCGCCATCTCTGTG
  TATGATCTCGTGGCTGTGCTGTGTCCCAAAGGGCCTCTGAGAATGCTGGTAGAAACTGCC
  CAGGAGAGAAATGAGCCCATATTCCCTGCCCTGATATACTCATCTGCCATGGTGTGGACG
  GTTGGCATGGCGAAGCTGGACCCCTCCTCTCAGGGTGCCCTCCAGCTCCCCTACGACCCG
  GAGATGGAAGAAGACTCCTATGACAGTTTTGGGGAGCCTTCATACCCCGAAGTCTTTGAG
  CCTCCCTTGACTGGCTACCCAGGGGAGGAGCTGGAGGAAGAGGAGGAAAGGGGCGTGAAG
  CTTGGCCTCGGGGACTTCATCTTCTACAGTGTGCTGGTGGGCAAGGCGGCTGCCACGGGC
  AGCGGGGACTGGAATACCACGCTGGCCTGCTTCGTGGCCATCCTCATTGGCTTGTGTCTG
  ACCCTCCTGCTGCTTGCTGTGTTCAAGAAGGCGCTGCCCGCCCTCCCCATCTCCATCACG
  TTCGGGCTCATCTTTTACTTCTCCACGGACAACCTGGTGCGGCCGTTCATGGACACCCTG
  GCCTCCCATCAGCTCTACATCTGAGGGACATGGTGTGCCACAGGCTGCAAGCTGCAGGGA
  ATTTTCATTGGATGCAGTTGTATAGTTTTACACTCTAGTGCCATATATTTTTAAGACTTT
  TCTTTCCTTAAAAAATAAAGTACGTGTTTACTTGGTGAGGAGGAGGCAGAACCAGCTCTT
  TGGTGCCAGCTGTTTCATCACCAGACTTTGGCTCCCGCTTTGGGGAGCGCCTCGCTTCAC
  GGACAGGAAGCACAGCAGGTTTATCCAGATGAACTGAGAAGGTCAGATTAGGGTGGGGAG
  AAGAGCATCCGGCATGAGGGCTGAGATGCGCAAAGAGTGTGCTCGGGAGTGGCCCCTGGC
  ACCTGGGTGCTCTGGCTGGAGAGGAAAAGCCAGTTCCCTACGAGGAGTGTTCCCAATGCT
  TTGTCCATGATGTCCTTGTTATTTTATTGCCTTTAGAAACTGAGTCCTGTTCTTGTTACG
  GCAGTCACACTGCTGGGAAGTGGCTTAATAGTAATATCAATAAATAGATGAGTCCTGTTA
  GAATCTTGAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000447 unedited
  NNNNGGGGGGNNNNNNNNCTCCCCNCNNNCGGTTCAAAATTGNATACGACTCATATAGGC
  GGCCGCGAAATTCGCACGAGGCAGAGCAGGCATTTCCAGCAGTGAGGAGACAGCCAGAAG
  CAAGCTTTTGGAGCTGAAGGAACCTGAGACAGAAGCTAGTCCCCCCTCTGAATTTTACTG
  ATGAAGAAACTGAGGCCACAGAGCTAAAGTGACTTTTCCCAAGGTCGCCCAGCGAGGACG
  TGGGACTTCTCAGACGTCAGGAGAGTGATGTGAGGGAGCTGTGTGACCATAGAAAGTGAC
  GTGTTAAAAACCAGCGCTGCCCTCTTTGAAAGCCAGGGAGCATCATTCATTTAGCCTGCT
  GAGAAGAAGAAACCAAGTGTCCGGGATTCAGACCTCTCTGCGGCCCCAAGTGTTCGTGGT
  GCTTCCAGAGGCAGGGCTATGCTCACATTCATGGCCTCTGACAGCGAGGAAGAAGTGTGT
  GATGAGCGGACGTCCCTAATGTCGGCCGAGAGCCCCACGCCGCGCTCCTGCCAGGAGGGC
  AGGCAGGGCCCAGAGGATGGAGAGAATACTGCCCAGTGGAGAAGCCAGGAGAACGAGGAG
  GACGGTGAGGAGGACCCTGACCGCTATGTCTGTAGTGGGGTTCCCGGGCGGNCGNCAGGC
  CTGGAGGAAGAGCTGACCCTCANATACNGAGCGAAGCATGTGATCATGCNTGTTGTGCCT
  GTCACTCTGTGCATGATCGTGGTGGTAGCCACCATCAAGTCTGTGCGCTTCTACACAGAG
  AAGAATGGACAGCTCATCTACACGCCATTCACTTGAGACACACCCCTCGTGGGGCAGCGC
  CTNCTCAACTNCGTGCTGAACACCCTCATCATGATCAGCGTCATCGTGGTTATGACCATC
  TTCTGGGTGTGCTCTACAGTACCGTGCTACAAGTCATCATGGCTGGNTGACATGTCTCAC
  TGAAGCTGCGGTCCTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000447 unedited
  TCCCGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGG
  AAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTCAAGA
  TTCTAACAGGACTCATCTATTTATTGATATTACTATTAAGCCACTTCCCAGCAGTGTGAC
  TGCCGTAACAAGAACAGGACTCAGTTTCTAAAGGCAATAAAATAACAAGGACATCATGGA
  CAAAGCATTGGGAACACTCCTCGTAGGGAACTGGCTTTTCCTCTCCAGCCAGAGCACCCA
  GGTGCCAGGGGCCACTCCCGAGCACACTCTTTGCGCATCTCAGCCCTCATGCCGGATGCT
  CTTCTCCCCACCCTAATCTGACCTTCTCAGTTCATCTGGATAAACCTGCTGTGCTTCCTG
  TCCGTGAAGCGAGGCGCTCCCCAAAGCGGGAGCCAAAGTCTGGTGATGAAACAGCTGGCA
  CCAAAGAGCTGGTTCTGCCTCCTCCTCACCAAGTAAACACGTACTTTATTTTTTAAGGAA
  AGAAAAGTCTTAAAAATATATGGCACTAGAGTGTAAAACTATACAACTGCATCCAATGAA
  AATTCCCTGCAGCTTGCAGCCTGTGGCACACCATGTCCCTCANATGTAGAGCTGATGGGA
  GGCCAGGGTGTCCATGAACGGCCGCACCAGGTTGTCCGTGGAGAAGTAAAAGATGAGCCC
  GAACGTGATGGANATGGNGAGGGCGGGCAGCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000447
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_000447.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000447.1, NP_000438.1
• RefSeq Size: 2236 bp
• RefSeq ORF: 1347 bp
• Locus ID: 5664
• Cytogenetics: 1q42.13
• Domains: Presenilin, PSN
• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Alzheimer's disease, Notch signaling pathway
• Gene Summary: 'Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).