HSD17B1 (NM_000413) Human Untagged Clone

CAT#: SC119873

HSD17B1 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 1 (HSD17B1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSD17B1
• Synonyms: 17-beta-HSD; 20-alpha-HSD; E2DH; EDH17B2; EDHB17; HSD17; SDR28C1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119873 sequence for NM_000413 edited (data generated by NextGen Sequencing)
  ATGGCCCGCACCGTGGTGCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGCACTTGGCC
  GTACGTCTGGCTTCAGATCCATCCCAGAGCTTCAAAGTGTATGCCACGTTGAGGGACCTG
  AAAACACAGGGCCGGCTGTGGGAGGCGGCCCGGGCCCTGGCATGCCCTCCGGGATCCCTG
  GAGACGTTGCAGCTGGACGTAAGGGACTCAAAATCCGTGGCCGCTGCCCGGGAACGCGTG
  ACTGAGGGCCGCGTGGACGTGCTGGTGTGTAACGCAGGCCTGGGCCTGCTGGGGCCGCTG
  GAGGCGCTGGGGGAGGACGCCGTGGCCTCTGTGCTGGACGTGAATGTAGTAGGGACTGTG
  CGGATGCTGCAGGCCTTCCTGCCAGACATGAAGAGGCGCGGTTCGGGACGCGTGTTGGTG
  ACCGGGAGCGTGGGAGGATTGATGGGGCTGCCTTTCAATGACGTTTATTGCGCCAGCAAG
  TTCGCGCTCGAAGGCTTATGCGAGAGTCTGGCGGTTCTGCTGCTGCCCTTTGGGGTCCAC
  TTGAGCCTGATCGAGTGCGGCCCAGTGCACACCGCCTTCATGGAGAAGGTGTTGGGCAGC
  CCAGAGGAGGTGCTGGACCGCACGGACATCCACACCTTCCACCGCTTCTACCAATACCTC
  GCCCACAGCAAGCAAGTCTTTCGCGAGGCGGCGCAGAACCCTGAGGAGGTGGCGGAGGTC
  TTCCTCACCGCTTTGCGCGCCCCGAAGCCGACCCTGCGCTACTTCACCACCGAGCGCTTC
  CTGCCCCTGCTGCGGATGCGCCTGGACGACCCCAGCGGCTCCAACTACGTCACCGCCATG
  CACCGGGAAGTGTTCGGCGACGTTCCGGCAAAGGCCGAGGCTGGGGCCGAGGCTGGGGGC
  GGGGCCGGGCCTGGGGCAGAGGACGAGGCCGGGCGCGGTGCGGTGGGGGACCCTGAGCTC
  GGCGATCCTCCGGCCGCCCCGCAGTAA
  
  Clone variation with respect to NM_000413.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000413 unedited
  ATAATTTGTAATACGACTCACTATAGGCGGCCGCGACATTCGCACCAGCTACAACCCACA
  ATCAGATTATAGTCCTGCCTTGGGCAGCTGAAAAAGGACAGGAGAAGGTCAGAGAGACGA
  AAGGCTGTTTTTTGAGGCCTGAGGCACCCCAACATGACAACGTAAGACTGTAACCATGGT
  CATGTGAGTTATGAGCTAGGAACCCTGGACGAAACCAGCACATATACAATCATCTCCCAA
  CTCCCAACGCCTTTACTTTCACAAGCCTCTGCAGCAAACTGCGGTCACTATAATCGCTCC
  TGTGGCACATAGGCATACCCAGGGGAATCTGCCCAGGGGGCCACTCTGTGCCCACGTGGG
  AACCCACACCTGCTTGTAAAGCCTCCCCTCCCTCTGACCAGCAACCAGGACAGTTTGTTG
  TTCCAAGCAGTGGGCTCATGTCTGTTTTGGCTCAAAACAGGGTGGGGAGAAGCGGGCCAG
  GGACCCGCATGAAGGCTTATCCTTGAGAATGCGTGGGAAACACAACAAGGGGTGGGGGCC
  CGCATGCGGNGCGGGGCGAATCATGTGATATCAAGCCCAGAGCCCCAGCCTCTCCCCACA
  GTCTCACCATGGCCCGCACCGTGGTGCTCATCACCGGCTGTTCCTCGGGCATCGGCCTGC
  ACTTGGCCGTACGTCTGGCTTCAGAACCATCCCAGAGCTTCAAAGTGTATGCCACGTTGA
  GGGACCTGAAACACANNNGCCGCTGTGGGATGCGGNCCNGGCCCTGCATGCCTNCGGNAT
  CCCTGGAAACGTGACNCTGACGTAAGGACTCANAATCGTGNCGCTGCCAGGAATCCTGAC
  TGAGGCGNNNTGACTGCTTGTGNAAAACCAAGCTGGCCTGCTGGGCCCTGAAGCCTTGGG
  GAAAGAACCTGGCTCTGCCTGAACTAATTNNANNNAGACTGGCGAATCTGAAGCTTCTGC
  ACATAAAAAAGCCGTGGGAACANNTTTTGACCGAAACGGGAGAAAATTAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000413 unedited
  GATCGAGTTATTTCCCTTTTTTTTTTTTTGATTTTTAAATTTTCTTTATTTTTGTAGAGG
  TGGGGGTCTCACTGTGTTGCTCTGGCTGGTCTCCAACTCCGGGGCTTGAGCGATCCTCCT
  GCCTCCGCCTCCCAAAGCGCTGGCATTACAGGCGACAGCCATCGCGCCTAGCTGCGCTGG
  TAAAACTGGCTAACGCGATCTATCTTAATTAGCCACCCACAGCTGCTACCGCCGCCCCAT
  CCCCAGGGACCACACAGACCCAGGGGACAAAGAAGGGCGCGGGAGACAGCGGCTGAGGAA
  GCCTTTACTGCGGGGCGGCCGGAGGATCGCCGAGCTCAGGGTCCCCCACCGCACCGCGCC
  CGGCCTCGTCCTCTGCCCCAGGCCCGGCCCCGCCCCCAGCCTCGGCCCCAGCCTCGGCCT
  TTGCCGGAACGTCGCCGAACACTTCCCGGTGCATGGCGGTGACCTATTTGGAGCCGCCTG
  GGTCGTTCAGGCGCATCCGCACCAGGGGCAAGAAGCGCTCGGGGGTGAAGTATCGCACGG
  TCGGCTTTGGCGCGCGCCAAGCGGTGAGGAAGACCTCCGCCACCTCCTCTAGGTTCTGCG
  CCGCCCTGCCGAAAGACTTGCCTTGCTGTGGCGCCGGTATTGGCCAGAACCCGGTGGAAG
  GTGCGGGATCTTCCTGTCGGGCCATCCCCCCTCTGCTGGGGTTGCCCAACCACCTTTTTC
  CTTGAAGGCGGGTGTTGCCTGGGCCCCGCCTCCCCCCAGGCTCAAGCTTCCGGACCCCAA
  AGGGCCCCCTCTCTCACCCCCCCGATCTCTTGTCATAACCCCCCGCGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000413
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000413.1, NP_000404.1
• RefSeq Size: 2230 bp
• RefSeq ORF: 987 bp
• Locus ID: 3292
• Cytogenetics: 17q21.2
• Domains: adh_short
• Protein Families: Druggable Genome
• Protein Pathways: Androgen and estrogen metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]'
  Transcript Variant: This variant (1) uses an alternate in-frame splice site in the 3' coding region, compared to variant 2. It encodes isoform 1, which is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.