Hydroxysteroid (17 beta) Dehydrogenase 4 (HSD17B4) (NM_000414) Human Untagged Clone

CAT#: SC119874

HSD17B4 (untagged)-Human hydroxysteroid (17-beta) dehydrogenase 4 (HSD17B4), transcript variant 2


  "NM_000414" in other vectors (4)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol HSD17B4
Synonyms DBP; MFE-2; MFP-2; MPF-2; PRLTS1; SDR8C1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119874 sequence for NM_000414 edited (data generated by NextGen Sequencing)
ATGGGCTCACCGCTGAGGTTCGACGGGCGGGTGGTACTGGTCACCGGCGCGGGGGCAGGA
TTGGGCCGAGCCTATGCCCTGGCTTTTGCAGAAAGAGGAGCGTTAGTTGTTGTGAATGAT
TTGGGAGGGGACTTCAAAGGAGTTGGTAAAGGCTCCTTAGCTGCTGATAAGGTTGTTGAA
GAAATAAGAAGGAGAGGTGGAAAAGCAGTGGCCAACTATGATTCAGTGGAAGAAGGAGAG
AAGGTTGTGAAGACAGCCCTGGATGCTTTTGGAAGAATAGATGTTGTGGTCAACAATGCT
GGAATTCTGAGGGATCGTTCCTTTGCTAGGATAAGTGATGAAGACTGGGATATAATCCAC
AGAGTTCATTTGCGGGGTTCATTCCAAGTGACACGGGCAGCATGGGAACACATGAAGAAA
CAGAAGTATGGAAGGATTATTATGACTTCATCAGCTTCAGGAATATATGGCAACTTTGGC
CAGGCCAATTATAGTGCTGCAAAGTTGGGTCTTCTGGGCCTTGCAAATTCTCTTGCAATT
GAAGGCAGGAAAAGCAACATTCATTGTAACACCATTGCTCCTAATGCGGGATCACGGATG
ACTCAGACAGTTATGCCTGAAGATCTTGTGGAAGCCCTGAAGCCAGAGTATGTGGCACCT
CTTGTCCTTTGGCTTTGTCACGAGAGTTGTGAGGAGAATGGTGGCTTGTTTGAGGTTGGA
GCAGGATGGATTGGAAAATTACGCTGGGAGCGGACTCTTGGAGCTATTGTAAGACAAAAG
AATCACCCAATGACTCCTGAGGCAGTCAAGGCTAACTGGAAGAAGATCTGTGACTTTGAG
AATGCCAGCAAGCCTCAGAGTATCCAAGAATCAACTGGCAGTATAATTGAAGTTCTGAGT
AAAATAGATTCAGAAGGAGGAGTTTCAGCAAATCATACTAGTCGTGCAACGTCTACAGCA
ACATCAGGATTTGCTGGAGCTATTGGCCAGAAACTCCCTCCATTTTCTTATGCTTATACG
GAACTGGAAGCTATTATGTATGCCCTTGGAGTGGGAGCGTCAATCAAGGATCCAAAAGAT
TTGAAATTTATTTATGAAGGAAGTTCTGATTTCTCCTGTTTGCCCACCTTCGGAGTTATC
ATAGGTCAGAAATCTATGATGGGTGGAGGATTAGCAGAAATTCCTGGACTTTCAATCAAC
TTTGCAAAGGTTCTTCATGGAGAGCAGTACTTAGAGTTATATAAACCACTTCCCAGAGCA
GGAAAATTAAAATGTGAAGCAGTTGTTGCTGATGTCCTAGATAAAGGATCCGGTGTAGTG
ATTATTATGGATGTCTATTCTTATTCTGAGAAGGAACTTATATGCCACAATCAGTTCTCT
CTCTTTCTTGTTGGCTCTGGAGGCTTTGGTGGAAAACGGACATCAGACAAAGTCAAGGTA
GCTGTAGCCATACCTAATAGACCTCCTGATGCTGTACTTACAGATACCACCTCTCTTAAT
CAGGCTGCTTTGTACCGCCTCAGTGGAGACTGGAATCCCTTACACATTGATCCTAACTTT
GCTAGTCTAGCAGGTTTTGACAAGCCCATATTACATGGATTATGTACATTTGGATTTTCT
GCCAGGCGTGTGTTACAGCAGTTTGCAGATAATGATGTGTCAAGATTCAAGGCAATTAAG
GCTCGTTTTGCAAAACCAGTATATCCAGGACAAACTCTACAAACTGAGATGTGGAAGGAA
GGAAACAGAATTCATTTTCAAACCAAGGTCCAAGAAACTGGAGACATTGTCATTTCAAAT
GCATATGTGGATCTTGCACCAACATCTGGTACTTCAGCTAAGACACCCTCTGAGGGCGGG
AAGCTTCAGAGTACCTTTGTATTTGAGGAAATAGGACGCCGCCTAAAGGATATTGGGCCT
GAGGTGGTGAAGAAAGTAAATGCTGTATTTGAGTGGCATATAACCAAAGGCGGAAATATT
GGGGCTAAGTGGACTATTGACCTGAAAAGTGGTTCTGGAAAAGTGTACCAAGGCCCTGCA
AAAGGTGCTGCTGATACAACAATCATACTTTCAGATGAAGATTTCATGGAGGTGGTCCTG
GGCAAGCTTGACCCTCAGAAGGCATTCTTTAGTGGCAGGCTGAAGGCCAGAGGGAACATC
ATGCTGAGCCAGAAACTTCAGATGATTCTTAAAGACTACGCCAAGCTCTGA

Clone variation with respect to NM_000414.3
>OriGene 5' read for NM_000414 unedited
NGGTCAGCATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCTTGTTCG
TGTGTGTGTCGTTGCAGGCCTTATTCATGGGCTCACCGCTGAGGTTCGACGGGCGGGTGG
TACTGGTCACCGGCGCGGGGGCAGGATTGGGCCGAGCCTATGCCCTGGCTTTTGCAGAAA
GAGGAGCGTTAGTTGTTGTGAATGATTTGGGAGGGGACTTCAAAGGAGTTGGTAAAGGCT
CCTTAGCTGCTGATAAGGTTGTTGAAGAAATAAGAAGGAGAGGTGGAAAAGCAGTGGCCA
ACTATGATTCAGTGGAAGAAGGAGAGAAGGTTGTGAAGACAGCCCTGGATGCTTTTGGAA
GAATAGATGTTGTGGTCAACAATGCTGGAATTCTGAGGGATCGTTCCTTTGCTAGGATAA
GTGATGAAGACTGGGATATAATCCACAGAGTTCATTTGCGGGGTTCATTCCAAGTGACAC
GGGCAGCATGGGAACACATGAAGAAACAGAAGTATGGAAGGATTATTATGACTTCATCAG
CTTCAGGAATATATGGCAACTTTGGCCAGGCCAATTATAGTGCTGCAAAGTTGGGTCTTC
TGGGCCTTGCAAATTCTCTTGCAATTGAAGGCAGGAAAAGCAACATTCATTGTAACACCA
TTGCTCCTAATGCGGGATCACGGATGACTCAGACAGTTATGCCTGAAGATCTTGTGGAAG
CCCTGAAGCCAGAGTATGTGGCACCTCTTGTCCTTTGGCTNTGTCACGAGAAGTGTGAGG
AGAATGGTGCCTTGTTTGAGGTTGGAGCCAGATGGATTGGAAATTACGCTNGGAGCGACT
CTTGNAGCTATTGTAGACAAAGATCACCCCATGACTCCTGAGCAGTCAAGGCTACTGGAG
AA
>OriGene 3' read for NM_000414 unedited
GAACGCGGCCGCAATCTATAGTCGAGTTTTTTTTTTTTTTTTTTTTTAAAGTATCAAAGG
CCTTTATTGATTTAATTTTCCTTTCAACTTATCAATGCTATCACAAATTCTAAGGAAACA
GGACTTGGGCAAAATTTTTTATTATGAAGATACAGATCTAAGAACAGAAGGATAATTATG
TGCTAGCTTATATATAGTTCCTTGTAAAAATAATGATACATGAAAAATTAGTAGAAAATG
AAAATCTGCAGTTATCTGATATCTGAAAATGTTAAGCAATTTCCCCTGCATCTTAGTTCT
AATCACTTTTGCAGAATAATCAAGCATATTTGGGTGAAGAGAGTATTTAATGATTCCATT
TTTATTAATAGTGTAGTGTGCCCTTCAGAGCTTGGCGTAGTCTTTAAGAATCATCTGAAG
TTTCTGGCTCAGCATGATGTTCCCTCTGGCCTTCAGCCTGCCACTAAAGAATGCCTTCTG
AGGGTCAAGCTTGCCCAGGACCACCTCCATGAAATCTTCATCTGAAAGTATGATTGTTGT
ATCAGCAGCACCTTTTGCAGGGCCTTGGTACACTTTTCCAGAACCACTTTTCAAGTCAAT
AGTCCACTTAGCCCCAATATTTCCGCCTTTGGTTATATGCCACTCAAATACAGCATTTAC
TTTTTTCACCACCTCATGCCCAATATCCTTTAAGCGGCGCCCCTATTCCTCAAATACAAA
AGTACTCTGAAACTTCCCCGCCTCAGAAGGTGTCTTAACTGGAGACCAGAAGTTGGGGCA
AGATCCCCATATGCATTTTGAATGACAATGTTTCCCACTCTCTTGAACCTTGGCTGGAAA
AAGAATTCTGTTTCCTTCCTTACACTTCTAATTTGAAAAAGCTGCCCTGGATATATGGTT
TTGACAACCAGCCTTATTGGCACTGATCTGGACACCTATTTTCGGAACTGCTGTACACAC
GCTGGTAAAATCACATGGCATAACCCGTCAATGGGCTTGACAAACGGGCACTAG
Restriction Sites NotI-NotI     
ACCN NM_000414
Insert Size 2590 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000414.1, NP_000405.1
RefSeq Size 2593 bp
RefSeq ORF 2211 bp
Locus ID 3295
Cytogenetics 5q23.1
Domains adh_short, MaoC_dehydratas, SCP2
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Primary bile acid biosynthesis
Gene Summary 'The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]'
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.

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