Myosin Light Chain 2 (MYL2) (NM_000432) Human Untagged Clone

CAT#: SC119884

MYL2 (untagged)-Human myosin, light chain 2, regulatory, cardiac, slow (MYL2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYL2
• Synonyms: CMH10; MLC-2s/v; MLC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000432 edited
  ATGGCACCTAAGAAAGCAAAGAAGAGAGCCGGGGGCGCCAACTCCAACGTGTTCTCCATG
  TTCGAACAGACCCAAATCCAGGAATTTAAGGAGGCCTTCACTATCATGGACCAGAACAGG
  GATGGCTTCATTGACAAGAACGATCTGAGAGACACCTTTGCTGCCCTTGGGCGAGTGAAC
  GTGAAAAATGAAGAAATTGATGAAATGATCAAGGAGGCTCCGGGTCCAATTAACTTTACT
  GTGTTCCTCACAATGTTTGGGGAGAAACTTAAGGGAGCGGACCCTGAGGAAACCATTCTC
  AACGCATTCAAAGTGTTTGACCCTGAAGGCAAAGGGGTGCTGAAGGCTGATTACGTTCGG
  GAAATGCTGACCACGCAGGCGGAGAGGTTTTCCAAGGAGGAGGTTGACCAGATGTTCGCC
  GCCTTCCCCCCTGACGTGACTGGCAACTTGGACTACAAGAACCTGGTGCACATCATCACC
  CACGGAGAAGAGAAGGACTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000432 unedited
  TTCAGGATTTGAATACNATTATATAGGGCGGCCGCGATTCGGCACGAGGCTCGGGAGGCA
  GTGCTGGGTCCTTTCCACCATGGCACCTAAGAAAGCAAAGAAGAGAGCCGGGGGCGCCAA
  CTCCAACGTGTTCTCCATGTTCGAACAGACCCAAATCCAGGAATTTAAGGAGGCCTTCAC
  TATCATGGACCAGAACAGGGATGGCTTCATTGACAAGAACGATCTGAGAGACACCTTTGC
  TGCCCTTGGGCGAGTGAACGTGAAAAATGAAGAAATTGATGAAATGATCAAGGAGGCTCC
  GGGTCCAATTAACTTTACTGTGTTCCTCACAATGTTTGGGGAGAAACTTAAGGGAGCGGA
  CCCTGAGGAAACCATTCTCAACGCATTCAAAGTGTTTGACCCTGAAGGCAAAGGGGTGCT
  GAAGGCTGATTACGTTCGGGAAATGCTGACCACGCAGGCGGAGAGGTTTTCCAAGGAGGA
  GGTTGACCAGATGTTCGCCGCCTTCCCCCCTGACGTGACTGGCAACTTGGACTACAAGAA
  CCTGGTGCACATCATCACCCACGGAGAAGAGAAGGACTAGGAGGGGGCTCGCTGCTGCGC
  CCTGNGCTCGTCTTTGCAGAGTGGTCCCTGCCCTCATCTCTCTCCCCCGAGTACCGCCTC
  TGTCCCCTACCTTGTCTGTTAGCCATGTGGCTGCCCCATTTATCCACCTCCATCTTCTTT
  GCAGCCTGNGTGGCTATGGGTACTTCGTGGGCGCACATCCTACAGTTGGAAAACCATCCC
  GAAGCCATGTTCCATTAAACCAGAGGTCGTGTATTTGGTCAAAAAAAAAAAAAAAACTCG
  ACTTTAATTGCGCCCGCGGCATTGCTGTTTCCTGAACAAACCCGGGTGGCATCCTTGGGA
  CCCTCCCCATGGCTTTTCTGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000432 unedited
  CGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGACCAAATACACGACCTCCTG
  TTTATTGGAACATGGCCTCTGTGAGGATTTCCAACTGTAGGATGTGCGGCCACGAAGTAC
  CCATAGCCACCCAGGCTGCAAAGAAGATGGAGGTGGATAAATGGGGCAGCCACATGGCTA
  ACAGACAAGGTAGGGACAGAGGCGGTACTCGGGGGAGAGAGATGAGGGCAGGGACCACTC
  TGCAAAGACGAGCCCAGGGCGCAGCAGCGAGCCCCCTCCTAGTCCTTCTCTTCTCCGTGG
  GTGATGATGTGCACCAGGTTCTTGTAGTCCAAGTTGCCAGTCACGTCAGGGGGGAAGGCG
  GCGAACATCTGGTCAACCTCCTCCTTGGAAAACCTCTCCGCCTGCGTGGTCAGCATTTCC
  CGAACGTAATCAGCCTTCAGCACCCCTTTGCCTTCAGGGTCAAACACTTTGAATGCGTTG
  AGAATGGTTTCCTCAGGGTCCGCTCCCTTAAGTTTCTCCCCAAACATTGTGAGGAACACA
  GTAAAGTTAATTGGACCCGGAGCCTTCTTGATCATTTCATCAATTTCTTCATTTTTCACG
  TTCACTCGCCCAAGGCCAGCAAAGGTGTCTCTCAGATCGTTCTTGCCAATGAAGCCATCC
  CCTGTCTCGTCCATGATAGTGAAGGCCTCCTTAAATTTCTGGATTTGGGTCCTGTTCGAC
  CATGGAGGACACGTTGGAGTTGGCGCCCCCCGTCTCTCTTCTTTGCTTTNNTTAGCNGCC
  ATGCTGGAAAGGACCAATACTGCCTCCCGATCCTGGGCCCGATTTTGGCCCGCCTTTGTG
  AGTTGTATTACCAATTTTGACGGTCACCTAACAACTCTGTTTAATAAACCTCCACCGTAC
  ACCCTACCGCCCATTTGGGCAAGCGGGCGGGTTATTACAAACTTTGGAAGCCCGTGAATT
  GGGGCCAACACTCCCTTGGACCCAGGGGTGGGAACTGTAACCCGGGGGCAAACGTTTTCC
  GCCCTTGGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000432
• Insert Size: 720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000432.1, NP_000423.1
• RefSeq Size: 813 bp
• RefSeq ORF: 501 bp
• Locus ID: 4633
• Cytogenetics: 12q24.11
• Domains: EFh
• Protein Pathways: Cardiac muscle contraction, Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction
• Gene Summary: 'Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]'