MRP2 (ABCC2) (NM_000392) Human Untagged Clone

CAT#: SC119897

ABCC2 (untagged)-Human ATP-binding cassette, sub-family C (CFTR/MRP), member 2 (ABCC2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ABCC2
• Synonyms: ABC30; CMOAT; cMRP; DJS; MRP2
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000392 edited
  GTCATCCTTTACGGAGAACATCAGAATGGTAGATAATTCCTGTTCCACTTTCTTTGATGA
  AACAAGTAAAGAAGAAACAACACAATCATATTAATAGAAGAGTCTTCGTTCCAGACGCAG
  TCCAGGAATCATGCTGGAGAAGTTCTGCAACTCTACTTTTTGGAATTCCTCATTCCTGGA
  CAGTCCGGAGGCAGACCTGCCACTTTGTTTTGAGCAAACTGTTCTGGTGTGGATTCCCTT
  GGGCTTCCTATGGCTCCTGGCCCCCTGGCAGCTTCTCCACGTGTATAAATCCAGGACCAA
  GAGATCCTCTACCACCAAACTCTATCTTGCTAAGCAGGTATTCGTTGGTTTTCTTCTTAT
  TCTAGCAGCCATAGAGCTGGCCCTTGTACTCACAGAAGACTCTGGACAAGCCACAGTCCC
  TGCTGTTCGATATACCAATCCAAGCCTCTACCTAGGCACATGGCTCCTGGTTTTGCTGAT
  CCAATACAGCAGACAATGGTGTGTACAGAAAAACTCCTGGTTCCTGTCCCTATTCTGGAT
  TCTCTCGATACTCTGTGGCACTTTCCAATTTCAGACTCTGATCCGGACACTCTTACAGGG
  TGACAATTCTAATCTAGCCTACTCCTGCCTGTTCTTCATCTCCTACGGATTCCAGATCCT
  GATCCTGATCTTTTCAGCATTTTCAGAAAATAATGAGTCATCAAATAATCCATCATCCAT
  AGCTTCATTCCTGAGTAGCATTACCTACAGCTGGTATGACAGCATCATTCTGAAAGGCTA
  CAAGCGTCCTCTGACACTCGAGGATGTCTGGGAAGTTGATGAAGAGATGAAAACCAAGAC
  ATTAGTGAGCAAGTTTGAAACGCACATGAAGAGAGAGCTGCAGAAAGCCAGGCGGGCACT
  CCAGAGACGGCAGGAGAAGAGCTCCCAGCAGAACTCTGGAGCCAGGCTGCCTGGCTTGAA
  CAAGAATCAGAGTCAAAGCCAAGATGCCCTTGTCCTGGAAGATGTTGAAAAGAAAAAAAA
  GAAGTCTGGGACCAAAAAAGATGTTCCAAAATCCTGGTTGATGAAGGCTCTGTTCAAAAC
  TTTCTACATGGTGCTCCTGAAATCATTCCTACTGAAGCTAGTGAATGACATCTTCACGTT
  TGTGAGTCCTCAGCTGCTGAAATTGCTGATCTCCTTTGCAAGTGACCGTGACACATATTT
  GTGGATTGGATATCTCTGTGCAATCCTCTTATTCACTGCGGCTCTCATTCAGTCTTTCTG
  CCTTCAGTGTTATTTCCAACTGTGCTTCAAGCTGGGTGTAAAAGTACGGACAGCTATCAT
  GGCTTCTGTATATAAGAAGGCATTGACCCTATCCAACTTGGCCAGGAAGGAGTACACCGT
  TGGAGAAACAGTGAACCTGATGTCTGTGGATGCCCAGAAGCTCATGGATGTGACCAACTT
  CATGCACATGCTGTGGTCAAGTGTTCTACAGATTGTCTTATCTATCTTCTTCCTATGGAG
  AGAGTTGGGACCCTCAGTCTTAGCAGGTGTTGGGGTGATGGTGCTTGTAATCCCAATTAA
  TGCGATACTGTCCACCAAGAGTAAGACCATTCAGGTCAAAAATATGAAGAATAAAGACAA
  ACGTTTAAAGATCATGAATGAGATTCTTAGTGGAATCAAGATCCTGAAATATTTTGCCTG
  GGAACCTTCATTCAGAGACCAAGTACAAAACCTCCGGAAGAAAGAGCTCAAGAACCTGCT
  GGCCTTTAGTCAACTACAGTGTGTAGTAATATTCGTCTTCCAGTTAACTCCAGTCCTGGT
  ATCTGTGGTCACATTTTCTGTTTATGTCCTGGTGGATAGCAACAATATTTTGGATGCACA
  AAAGGCCTTCACCTCCATTACCCTCTTCAATATCCTGCGCTTTCCCCTGAGCATGCTTCC
  CATGATGATCTCCTCCATGCTCCAGGCCAGTGTTTCCACAGAGCGGCTAGAGAAGTACTT
  GGGAGGGGATGACTTGGACACATCTGCCATTCGACATGACTGCAATTTTGACAAAGCCAT
  GCAGTTTTCTGAGGCCTCCTTTACCTGGGAACATGATTCGGAAGCCACAGTCCGAGATGT
  GAACCTGGACATTATGGCAGGCCAACTTGTGGCTGTGATAGGCCCTGTCGGCTCTGGGAA
  ATCCTCCTTGATATCAGCCATGCTG:GGAGAAATGGAAAATGTCCACGGGCACATCACCA
  TCAAGGGCACCACTGCCTATGTCCCACAGCAGTCCTGGATTCAGAATGGCACCATAAAGG
  ACAACATCCTTTTTGGAACAGAGTTTAATGAAAAGAGGTACCAGCAAGTACTGGAGGCCT
  GTGCTCTCCTCCCAGACTTGGAAATGCTGCCTGGAGGAGATTTGGCTGAGATTGGAGAGA
  AGGGTATAAATCTTAGTGGGGGTCAGAAGCAGCGGATCAGCCTGGCCAGAGCTACCTACC
  AAAATTTAGACATCTATCTTCTAGATGACCCCCTGTCTGCAGTG:GATGCTCATGTAGGA
  AAACATATTTTTAATAAGGTCTTGGGCCCCAATGGCCTGTTGAAAGGCAAGACTCGACTC
  TTGGTTACACATAGCATGCACTTTCTTCCTCAAGTGGATGAGATTGTAGTTCTGGGGAAT
  GGAACAATTGTAGAGAAAGGATCCTACAGTGCTCTCCTGGCCAAAAAAGGAGAGTTTGCT
  AAGAATCTGAAGACATTTCTAAGACATACAGGCCCTGAAGAGGAAGCCACAGTCCATGAT
  GGCAGTGAAGAAGAAGACGATGACTATGGGCTGATATCCAGTGTGGAAGAGATCCCCGAA
  GATGCAGCCTCCATAACCATGAGAAGAGAGAACAGCTTTCGTCGAACACTTAGCCGCAGT
  TCTAGGTCCAATGGCAGGCATCTGAAGTCCCTGAGAAACTCCTTGAAAACTCGGAATGTG
  AATAGCCTGAAGGAAGACGAAGAACTAGTGAAAGGACAAAAACTAATTAAGAAGGAATTC
  ATAGAAACTGGAAAGGTGAAGTTCTCCATCTACCTGGAGTACCTACAAGCAATAGGATTG
  TTTTCGATATTCTTCATCATCCTTGCGTTTGTGATGAATTCTGTGGCTTTTATTGGATCC
  AACCTCTGGCTCAGTGCTTGGACCAGTGACTCTAAAATCTTCAATAGCACCGACTATCCA
  GCATCTCAGAGGGACATGAGAGTTGGAGTCTACGGAGCTCTGGGATTAGCCCAAGGTATA
  TTTGTGTTCATAGCACATTTCTGGAGTGCCTTTGGTTTCGTCCATGCATCAAATATCTTG
  CACAAGCAACTGCTGAACAATATCCTTCGAGCACCTATGAGATTTTTTGACACAACACCC
  ACAGGCCGGATTGTGAACAGGTTTGCCGGCGATATTTCCACAGTGGATGACACCCTGCCT
  CAGTCCTTGCGCAGCTGGATTACATGCTTCCTGGGGATAATCAGCACCCTTGTCATGATC
  TGCATGGCCACTCCTGTCTTCACCATCATCGTCATTCCTCTTGGCATTATTTATGTATCT
  GTTCAGATGTTTTATGTGTCTACCTCCCGCCAGCTGAGGCGTCTGGACTCTGTCACCAGG
  TCCCCAATCTACTCTCACTTCAGCGAGACCGTATCAGGTTTGCCAGTTATCCGTGCCTTT
  GAGCACCAGCAGCGATTTCTGAAACACAATGAGGTGAGGATTGACACCAACCAGAAATGT
  GTCTTTTCCTGGATCACCTCCAACAGGTGGCTTGCAATTCGCCTGGAGCTGGTTGGGAAC
  CTGACTGTCTTCTTTTCAGCCTTGATGATGGTTATTTATAGAGATACCCTAAGTGGGGAC
  ACTGTTGGCTTTGTTCTGTCCAATGCACTCAATATCACACAAACCCTGAACTGGCTGGTG
  AGGATGACATCAGAAATAGAGACCAACATTGTGGCTGTTGAGCGAATAACTGAGTACACA
  AAAGTGGAAAATGAGGCACCCTGGGTGACTGATAAGAGGCCTCCGCCAGATTGGCCCAGC
  AAAGGCAAGATCCAGTTTAACAACTACCAAGTGCGGTACCGACCTGAGCTGGATCTGGTC
  CTCAG::::AGGGATCACTTGTG:::ACATCGGTAGCATGGAGAAGATTGGTGTGGTGGG
  CAGGACAGGAGCTGGAAAGTCATCCCTCACAAACTGCCTCTTCAGAATCTTAGAGGCTGC
  CGGTGGTCAGATTATCATTGATGGAGTAGATATTGCTTCCATTGGGCTCCACGACCTCCG
  AGAGAAGCTGACCATCATCCCCCAGGACCCCATCCTGTTCTCTGGAAGCCTGAGGATGAA
  TCTCGACCCTTTCAACAACTACTCAGATGAGGAGATTTGGAAGGCCTTGGAGCTGGCTCA
  CCTCAAGTCTTTTGTGGCCAGCCTGCAACTTGGGTTATCCCACGAAGTGACAGAGGCTGG
  TGGCAACCTGAGCATAGGCCAGAGGCAGCTGCTGTGCCTGGGCAGGGCTCTGCTTCGGAA
  ATCCAAGATCCTGGTCCTGGATGAGGCCACTGCTGCGGTGGATCTAGAGACAGACAACCT
  CATTCAGACGACCATCCAAAACGAGTTCGCCCACTGCACAGTGATCACCATCGCCCACAG
  GCTGCACACCATCATGGACAGTGACAAGGTAATGGTCCTAGACAACGGGAAGATTATAGA
  GTGCGGCAGCCCTGAAGAACTGCTACAAATCCCTGGACCCTTTTACTTTATGGCTAAGGA
  AGCTGGCATTGAGAATGTGAACAGCACAAAATTCTAGCAGAAGGCCCCATGGGTTAGAAA
  AGGACTATAAGAATAATTTCTTATTTAATTTTATTTTTTATAAAATACAGAATACATACA
  AAAGTGTGTATAAAATGTACGTTTTAAAAAAGGATAAGTGAACACCCATGAACCTACTAC
  CCAGGTTAAGAAAATAAATGTCACCAGGTACTTGAGAAACCCCTCGATTGTCTACCTCGA
  TCGTACTTCCTTGCTACCCACCCCTCCCAGGGACAACCACTGTCCTGAATTTCACGATAA
  TTATTCCTTTGCCTTTCATTTCTGTTTTATCACCTTTGTATGTATCTTTAAACAACATAT
  ACCCTTTTTTACTTATGTAAATGGACTGACTCATACTGCATACATCTTCTATGACTTGAA
  AAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000392
• Insert Size: 5200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: TrueClone
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000392.1, NP_000383.1
• RefSeq Size: 4868 bp
• RefSeq ORF: 4638 bp
• Locus ID: 1244
• Cytogenetics: 10q24.2
• Domains: ABC_membrane, ABC_tran, AAA
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• Gene Summary: 'The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia. [provided by RefSeq, Jul 2008]'