Cathepsin K (CTSK) (NM_000396) Human Untagged Clone

CAT#: SC119901

CTSK (untagged)-Human cathepsin K (CTSK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CTSK
• Synonyms: CTS02; CTSO; CTSO1; CTSO2; PKND; PYCD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119901 sequence for NM_000396 edited (data generated by NextGen Sequencing)
  ATGTGGGGGCTCAAGGTTCTGCTGCTACCTGTGGTGAGCTTTGCTCTGTACCCTGAGGAG
  ATACTGGACACCCACTGGGAGCTATGGAAGAAGACCCACAGGAAGCAATATAACAACAAG
  GTGGATGAAATCTCTCGGCGTTTAATTTGGGAAAAAAACCTGAAGTATATTTCCATCCAT
  AACCTTGAGGCTTCTCTTGGTGTCCATACATATGAACTGGCTATGAACCACCTGGGGGAC
  ATGACCAGTGAAGAGGTGGTTCAGAAGATGACTGGACTCAAAGTACCCCTGTCTCATTCC
  CGCAGTAATGACACCCTTTATATCCCAGAATGGGAAGGTAGAGCCCCAGACTCTGTCGAC
  TATCGAAAGAAAGGATATGTTACTCCTGTCAAAAATCAGGGTCAGTGTGGTTCCTGTTGG
  GCTTTTAGCTCTGTGGGTGCCCTGGAGGGCCAACTCAAGAAGAAAACTGGCAAACTCTTA
  AATCTGAGTCCCCAGAACCTAGTGGATTGTGTGTCTGAGAATGATGGCTGTGGAGGGGGC
  TACATGACCAATGCCTTCCAATATGTGCAGAAGAACCGGGGTATTGACTCTGAAGATGCC
  TACCCATATGTGGGACAGGAAGAGAGTTGTATGTACAACCCAACAGGCAAGGCAGCTAAA
  TGCAGAGGGTACAGAGAGATCCCCGAGGGGAATGAGAAAGCCCTGAAGAGGGCAGTGGCC
  CGAGTGGGACCTGTCTCTGTGGCCATTGATGCAAGCCTGACCTCCTTCCAGTTTTACAGC
  AAAGGTGTGTATTATGATGAAAGCTGCAATAGCGATAATCTGAACCATGCGGTTTTGGCA
  GTGGGATATGGAATCCAGAAGGGAAACAAGCACTGGATAATTAAAAACAGCTGGGGAGAA
  AACTGGGGAAACAAAGGATATATCCTCATGGCTCGAAATAAGAACAACGCCTGTGGCATT
  GCCAACCTGGCCAGCTTCCCCAAGATGTGA
  
  Clone variation with respect to NM_000396.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000396 unedited
  GGTTCGACGATATTTGAACACGATCTCACTATAGGGCGGCCGCGAATTCGCACGAGGGAA
  ACGAAGCCAGACAACAGATTTCCATCAGCAGGATGTGGGGGCTCAAGGTTCTGCTGCTAC
  CTGTGGTGAGCTTTGCTCTGTACCCTGAGGAGATACTGGACACCCACTGGGAGCTATGGA
  AGAAGACCCACAGGAAGCAATATAACAACAAGGTGGATGAAATCTCTCGGCGTTTAATTT
  GGGAAAAAAACCTGAAGTATATTTCCATCCATAACCTTGAGGCTTCTCTTGGTGTCCATA
  CATATGAACTGGCTATGAACCACCTGGGGGACATGACCAGTGAAGAGGTGGTTCAGAAGA
  TGACTGGACTCAAAGTACCCCTGTCTCATTCCCGCAGTAATGACACCCTTTATATCCCAG
  AATGGGAAGGTAGAGCCCCAGACTCTGTCGACTATCGAAAGAAAGGATATGTTACTCCTG
  TCAAAAATCAGGGTCAGTGTGGTTCCTGTTGGGCTTTTAGCTCTGTGGGTGCCCTGGAGG
  GCCAACTCAAGAAGAAAACTGGCAAACTCTTAAATCTGAGTCCCCAGAACCTAGTGGATT
  GTGTGTCTGAGAATGATGGCTGTGGAGGGGGCTACATGACCAATGCCTTCCAATATGTGC
  AGAAGAACCGGGGTATTGACTCTGAAGATGCCTACCCATATGTGGGACAGGAAGAGAGTT
  GTATGTACAACCCAACAGGCAAGGCAGCTAAATGCANGAGGTACAGAGAGATCCCCCGAG
  GGAAATGAGAAGCCCTGAAGAAGGCAGTGGCCCCGAGTGGGACCTGTCTCTGTGGCCATT
  TGATGCAGCCTGCACTCCTTTCAGTTTACAGCAAAAGTGTGTATTATGATGAAAGCTGCC
  ATAGCGATAATCTGAACCCATGCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000396 unedited
  ACAGGGAGCAAAATACTTTATTTCAAAAGTGCAAAGAAGGGGAAGACAAATCACATTTCT
  TTTTATCATGTAAACTTGTACCAAAGACTTATGAATAGATTTATGAATTGGAGTAGAAAA
  CATAGACATTTCTACCTTGAGGATATAGAAGGGAACTTAGGAAGTGAGAAGTCAGATTAC
  CCTAGTCCTCTAGGGTGCTTAAAGCTAACTAGTCCCGTGAATGAGAATCTAACCTATGTG
  AAAATCTCCAGCCTGTACCTGTACAGCATCAGCCCTGGGACAACACAGTCAGGGGCAGGC
  TGTAGTCACATCTCTTAGCCTAAGAGTACACAGCTGTCAGGGAAAGTCCTGATGGCCACA
  GTGAAAAAGGTCATGGGTGGAGAGAAGCAAAGTAGGAAGGATCATTTGAAGCACAAACAA
  ATGGGGAAACTGAACAGACAATCTCAGTATCACCACATCTGCTTCAAAAATAGCACACCA
  ACTCCCTTCCAAAGTGCATCGTTACACTGCACCATCGTGGAAGATATGGAAGAGCANGAT
  GGATTTGGCTGGCTGGAGTCACATCTTGGGGAAGCTGGCCAGGTTGGCATGCCACAGGCG
  TTGTCCTATTTCGAGCCATGAGAATATCCTTTGTTTCCCAGTTTTCTCCCAGCTGTTTTT
  ATTATCCAGTGCTTGTTCCCTTCTGATTCCTATCCCACTGCAAACCGCAGGTTCAGATAT
  CGTTATGCAGCTTTATCATATACACCACTTGCTGAAAACTGGAAGAGGCAGGCTGGATCA
  ATGGCCCAAACCCGGTCCCACTGGGCCCTGCCCTTTTAGGGCCTTTTAATTCCCCTGGGG
  ACCTTTTTGACCCCTTCCTTTAATTGGCTCGCCTGGNGGGGTGACAAACACCTTTTTCCT
  GGCCAACTGTGGGAGGCATTTATGATACAACACCCCGCTCTTTGCACATTGTGAAGGATG
  GTCCTTGGCCCCCTCCCAGATTTTTCTCAACACATATCCACAGTTTGGGGATCCAATAAG
  AAGTCACATCTTCCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000396
• Insert Size: 1730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000396.2, NP_000387.1
• RefSeq Size: 1702 bp
• RefSeq ORF: 990 bp
• Locus ID: 1513
• Cytogenetics: 1q21.3
• Domains: Pept_C1
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Lysosome
• Gene Summary: 'The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013]'