GLB1 (NM_000404) Human Untagged Clone

CAT#: SC119908

GLB1 (untagged)-Human galactosidase, beta 1 (GLB1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GLB1
• Synonyms: EBP; ELNR1; MPS4B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119908 sequence for NM_000404 edited (data generated by NextGen Sequencing)
  ATGCCGGGGTTCCTGGTTCGCATCCTCCCTCTGCTGCTGGTTCTGCTGCTTCTGGGCCCT
  ACGCGCGGCTTGCGCAATGCCACCCAGAGGATGTTTGAAATTGACTATAGCCGGGACTCC
  TTCCTCAAGGATGGCCAGCCATTTCGCTACATCTCAGGAAGCATTCACTACTCCCGTGTG
  CCCCGCTTCTACTGGAAGGACCGGCTGCTGAAGATGAAGATGGCTGGGCTGAACGCCATC
  CAGACGTATGTGCCCTGGAACTTTCATGAGCCCTGGCCAGGACAGTACCAGTTTTCTGAG
  GACCATGATGTGGAATATTTTCTTCGGCTGGCTCATGAGCTGGGACTGCTGGTTATCCTG
  AGGCCCGGGCCCTACATCTGTGCAGAGTGGGAAATGGGAGGATTACCTGCTTGGCTGCTA
  GAGAAAGAGTCTATTCTTCTCCGCTCCTCCGACCCAGATTACCTGGCAGCTGTGGACAAG
  TGGTTGGGAGTCCTTCTGCCCAAGATGAAGCCTCTCCTCTATCAGAATGGAGGGCCAGTT
  ATAACAGTGCAGGTTGAAAATGAATATGGCAGCTACTTTGCCTGTGATTTTGACTACCTG
  CGCTTCCTGCAGAAGCGCTTTCGCCACCATCTGGGGGATGATGTGGTTCTGTTTACCACT
  GATGGAGCACATAAAACATTCCTGAAATGTGGGGCCCTGCAGGGCCTCTACACCACGGTG
  GACTTTGGAACAGGCAGCAACATCACAGATGCTTTCCTAAGCCAGAGGAAGTGTGAGCCC
  AAAGGACCCTTGATCAATTCTGAATTCTATACTGGCTGGCTAGATCACTGGGGCCAACCT
  CACTCCACAATCAAGACCGAAGCAGTGGCTTCCTCCCTCTATGATATACTTGCCCGTGGG
  GCGAGTGTGAACTTGTACATGTTTATAGGTGGGACCAATTTTGCCTATTGGAATGGGGCC
  AACTCACCCTATGCAGCACAGCCCACCAGCTACGACTATGATGCCCCACTGAGTGAGGCT
  GGGGACCTCACTGAGAAGTATTTTGCTCTGCGAAACATCATCCAGAAGTTTGAAAAAGTA
  CCAGAAGGTCCTATCCCTCCATCTACACCAAAGTTTGCATATGGAAAGGTCACTTTGGAA
  AAGTTAAAGACAGTGGGAGCAGCTCTGGACATTCTGTGTCCCTCTGGGCCCATCAAAAGC
  CTTTATCCCTTGACATTTATCCAGGTGAAACAGCATTATGGGTTTGTGCTGTACCGGACA
  ACACTTCCTCAAGATTGCAGCAACCCAGCACCTCTCTCTTCACCCCTCAATGGAGTCCAC
  GATCGAGCATATGTTGCTGTGGATGGGATCCCCCAGGGAGTCCTTGAGCGAAACAATGTG
  ATCACTCTGAACATAACAGGGAAAGCTGGAGCCACTCTGGACCTTCTGGTAGAGAACATG
  GGACGTGTGAACTATGGTGCATATATCAACGATTTTAAGGGTTTGGTTTCTAACCTGACT
  CTCAGTTCCAATATCCTCACGGACTGGACGATCTTTCCACTGGACACTGAGGATGCAGTG
  CGCAGCCACCTGGGGGGCTGGGGACACCGTGACAGTGGCCACCATGATGAAGCCTGGGCC
  CACAACTCATCCAACTACACGCTCCCGGCCTTTTATATGGGGAACTTCTCCATTCCCAGT
  GGGATCCCAGACTTGCCCCAGGACACCTTTATCCAGTTTCCTGGATGGACCAAGGGCCAG
  GTCTGGATTAATGGCTTTAACCTTGGCCGCTATTGGCCAGCCCGGGGCCCTCAGTTGACC
  TTGTTTGTGCCCCAGCACATCCTGATGACCTCGGCCCCAAACACCATCACCGTGCTGGAA
  CTGGAGTGGGCACCCTGCAGCAGTGATGATCCAGAACTATGTGCTGTGACGTTCGTGGAC
  AGGCCAGTTATTGGCTCATCTGTGACCTACGATCATCCCTCCAAACCTGTTGAAAAAAGA
  CTCATGCCCCCACCCCCGCAAAAAAACAAAGATTCATGGCTGGACCATGTATGA
  
  Clone variation with respect to NM_000404.2
  34 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000404 unedited
  ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCAC
  GAGGGTCAAGTGACGCGAAGCGGCCGGCCTGGGCGCCGACTGCAGAGCCGGGAGGCTGGT
  GGTCATGCCGGGGTTCCTGGTTCGCATCCTCCCTCTGCTGCTGGTTCTGCTGCTTCTGGG
  CCCTACGCGCGGCTTGCGCAATGCCACCCAGAGGATGTTTGAAATTGACTATAGCCGGGA
  CTCCTTCCTCAAGGATGGCCAGCCATTTCGCTACATCTCAGGAAGCATTCACTACTCCCG
  TGTGCCCCGCTTCTACTGGAAGGACCGGCTGCTGAAGATGAAGATGGCTGGGCTGAACGC
  CATCCAGACGTATGTGCCCTGGAACTTTCATGAGCCCTGGCCAGGACAGTACCAGTTTTC
  TGAGGACCATGATGTGGAATATTTTCTTCGGCTGGCTCATGAGCTGGGACTGCTGGTTAT
  CCTGAGGCCCGGGCCCTACATCTGTGCAGAGTGGGAAATGGGAGGATTACCTGCTTGGCT
  GCTAGAGAAAGAGTCTATTCTTCTCCGCTCCTCCGACCCAGATTACCTGNCAGCTGTGGA
  CAAGTGGTTGGGAGTCCTTCTGCCCAGATGAAGCCTCTCCTCTATCAGAATGGAGGNCCA
  GTTATAACAGTGCAGGTTGAAAATGAATATGGCAGCTACTTTGCCTGTGANTTTGACTAC
  CTGCGCTTCCTGCAGAAGCGCTTTCGNCACCATCTGGNGGATGGATGTGGGTCTGTTTAC
  CACTGATGGAGCACATAAAACATTCTGAATGTGGGGCCCTGCAGGGCCTCTACACCACGT
  GGACTTTTGAACCAGCAGCAACATACAGATGCTTTCCTAGGCAGAGAAGTGGGAGCCCAA
  GCACCTGGAACATTCTGATCTATACTGCTGCTAGACATTGGGGCANCTTACTCCAATCAG
  ACCAAGAAGGTTCCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000404 unedited
  GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGTACAAATTTTATTTAACAAAAAG
  GTAACATGATTCTTCCAAAATAAAAATCACTACCACCTTTAAAGCTTCCATTCCAGCCCT
  GCAGATATGTATGCACGTTACTGTGCTGTCAGCCCCTCACACATTCCAGGTGGTCCCTGA
  AGGTGGGGCTTTGGCACTGCAGGGATGCAGGGAAACCTCAGGTGAAAATGCACATCCTAA
  ATTCCTTTTCCATTTCCACATTCCAATCAGTGAAATGTGGCATGACAGGGAGGATCTGTG
  AGGTATGTTCAGGGTAGAATCCCTCAAAGACACAGGCTTTCATCATCATACATGGTCCAG
  CCATGAATCTTTGTTTTTTTGCGGGGGTGGGGGCATGAGTCTTTTTTCAACAGGTTTGGA
  GGGATGATCGTAGGTCACAGATGAGCCAATAACTGGCCTGTCCACGAACGTCACAGCACA
  TAGTTCTGGATCATCACTGCTGCAGGGTGCCCACTCCAGTTCCAGCACGGTGATGGTGTT
  TGGGGCCGACGTCATCAGGATGTGCTGGGGCACAAACAAGGTCAACTGAGGGCCCCGGGC
  TGGCCAATAGCGGCCAAGGTTAAAGCCATTAATCCAGACCTGGCCCTTGGTCCATCCAGG
  AAACTGGATAAAGGTGTCCTGGGGCAAGTCTGGGATCCCACTGGGAATGGAGAAGTTCCC
  ATATAAAAGCCGGGACGTGTANTTGATGAACTGTGGCCCAGGCTCATCATGTGGCACTGT
  ACGGTGGCCCACCCCCCAGGTGCTGCCCCTGATTCTACTGTCCCGGGAAGATTTCCCATC
  CTGAGATATTGGACTGAAATAACGTAAAACCAACCCTTAAATCTTGATATATGCCCTATT
  CACCCGTCATGTTCCTACAAAAGCCCAAAGGTTCAGTTTCCTTTTCCCGAGGAACACTGG
  TTGCTAAGCTCCCTGGATACCCCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000404
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000404.1, NP_000395.1
• RefSeq Size: 2409 bp
• RefSeq ORF: 2034 bp
• Locus ID: 2720
• Cytogenetics: 3p22.3
• Domains: Glyco_hydro_35
• Protein Families: Druggable Genome
• Protein Pathways: Galactose metabolism, Glycosaminoglycan degradation, Glycosphingolipid biosynthesis - ganglio series, Lysosome, Metabolic pathways, Other glycan degradation, Sphingolipid metabolism
• Gene Summary: 'This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome. [provided by RefSeq, Nov 2015]'
  Transcript Variant: This variant (1) encodes the predominant isoform (a).