HLCS (NM_000411) Human Untagged Clone

CAT#: SC119915

HLCS (untagged)-Human holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS)


  "NM_000411" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol HLCS
Synonyms HCS
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119915 sequence for NM_000411 edited (data generated by NextGen Sequencing)
ATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCAC
TTGCAGGACTCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATC
CTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCTGAGCAGGACGGTATGGAGCAT
GTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGAACCCAAACAAAGGAGAGGCAGTGCC
TCTGGGAGTGAGCCTGCTGGGGACAGTGACAGGGGAGGGGGCCCCGTTGAGCATTATCAC
CTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAGAACAGCACCATTGAGTCAGTC
AAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGTTTGGAG
AGTGTTGCTGATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAG
GCACCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAGGAAGCCCTCGGCCGGTTCCAC
GAGGTCCGGTCTGTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTG
CTGGAGGACAGTGCTCTCAGAGACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCT
ACCAGGGAGTCCATTCCCGAAGACCTGTACCAGAAGTTCATGGCCTATCTTTCTCAGGGA
GGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGTGGCTTTCAGGTGACAAGCAAG
GGTGCACTGCACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGACCAGAGYGAGGTG
AAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAAGGCCCCGTCCGGCTCAGCCCC
GGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCT
TTTGGAACTCGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGC
TCCAACATAGTGCAAACTCCAGAAGATTTTAACTTGCTCAAGTCAAGCAATTTTAGAAGA
TACGAAGTCCTTAGAGAGATTCTGACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTT
CCTGCCTTAACTCCTCTTTACTTGCTGTCAGCTGCGGAGGAAATCAGGGATCCTCTTATG
CAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATAAAATCCGGCCAGCTCTCTCTT
AGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATACCTGTGGTG
ACCAACATGGAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTG
CAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAAGTGACCCCCACAACGATGCGT
CTCCTGGATGGGCTGATGTTTCAGACACCGCAGGAAATGGGCTTAATAGTGATCGCGGCC
CGGCAGACCGAGGGCAAAGGACGGGGAGGGAATGTGTGGCTGAGCCCTGTGGGATGTGCT
CTTTCTACTCTGCTCATCTCCATTCCACTGAGATCCCAGCTGGGACAGAGGATCCCGTTT
GTCCAGCATCTGATGTCCGTGGCTGTCGTGGAAGCAGTGAGGTCCATTCCCGAGTATCAG
GATATCAACTTACGAGTGAAGTGGCCCAACGATATTTATTACAGTGACCTCATGAAGATC
GGCGGAGTTCTGGTTAACTCAACACTCATGGGAGAAACATTTTATATACTTATTGGCTGT
GGATTTAATGTGACTAACAGTAACCCTACCATCTGCATCAACGACCTCATCACAGAATAC
AATAAACAACACAAGGCAGAACTGAAGCCCTTAAGAGCCGATTATCTCATCGCCAGAGTC
GTGACTGTGCTGGAGAAACTGATCAAAGAGTTTCAGGACAAAGGGCCCAACAGCGTCCTT
CCCCTTTATTACCGATACTGGGTCCACAGTGGTCAGCAAGTCCATCTGGGCAGCGCAGAG
GGACCAAAGGTGTCCATCGTTGGCCTGGACGATTCTGGCTTCCTCCAGGTTCACCAGGAG
GGCGGCGAGGTTGTGACTGTGCACCCGGACGGCAACTCCTTCGACATGCTGAGAAACCTC
ATCCTCCCCAAACGGCGGTAA

Clone variation with respect to NM_000411.6
834 c=>y
>OriGene 5' read for NM_000411 unedited
GTTCACATTCGGCACCAGGACGGGGCTGGGGCGCGGGCAGGATTTGGGGCTGCGCCGAGG
GCGTCCCGACCTGGCCCTTTGCCACGGTAGGTTCCGGCTCCCATGCCTCCGCTGCCATCT
GCGCTGTCCTTGGCGACTACGCCTTCACCCCCCTGGGTGCGTTTCAGCGGTCCCGGAGCC
TGGCGGGAGCGGGGCCAGGAGCGCTCCGCGGCCGGTATCAGGCCTGCAGTCCATTGAAGA
CTTGAACAAGTGGGCCCTATTTCTTGTGTCTCCTTTTATACTTGAAGCAGAACACATAGC
ATTTGTGACGGAGAGCATTTGGGTACAAAGTGAGAATTTACAGAGATCATCCTCTTCAGA
AACAGTTCGGTTTTTGCCCACTAGGGATGATGTGGTTTCTCATGAGGTTACTTGCTCTAA
AGGACTTTATATTTTGGAACCATAAGAGCACCCTTGTGGCCCAGGCACTTTATGGATGAT
CCCTTTTAGTGCTCCCAGTAACCTTCCAAGATTGTCAAGTGGTCAGACTGTTGTTTGCCA
TTAGCTTGCAGACCTGGGGATCCTTATCGGCTAATTGCTGAAGCAAGTGTGGACAACTTC
AGCAAGCTGGGGGTGGCGTTCATGGAAGATAGACTCCACATGGATAATGGACTGGTACCC
CAAAAGATTGTGTCGGTGCACTTGCAGGACTCCACTCTGAAGGAAGTTAAGGATCAGGTC
TCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCT
GAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGACCCC
AAACCAAGGAGAGCAGTGCCTCTGGGAGTGACCTGCTGGGACAGTGACAGGGAAGGGGGC
CCCGTTGAGCATATCACCCTC
>OriGene 3' read for NM_000411 unedited
GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACAAATGAATTGGAG
GAAAAGAAAATTCACCTACAACTCTAAATTAGATTTCCAGATGCATGGGCACGGACAGGC
AGCCGCGTCTCGGGGACGCCCGGCATTACCGCCGTTTGGGGAGGATGAGGTTTCTCAGCA
TGTCGAAGGAGTTGCCGTCCGGGTGCACAGTCACAACCTCGCCGCCCTCCTGGTGAACCT
GGAGGAAGCCAGAATCGTCCAGGCCAACGATGGACACCTTTGGTCCCTCTGCGCTGCCCA
GATGGACTTGCTGACCACTGTGGACCCAGTATCGGTAATAAAGGGGAAGGACGCTGTTGG
GCCCTTTGTCCTGAAACTCTTTGATCAGTTTCTCCAGCACAGTCACGACTCTGGCGATGA
GATAATCGGCTCTTAAGGGCTTCAGTTCTGCCTTGTGTTGTTTATTGTATTCTGTGATGA
GGTCGTTGATGCAAATGGTAGGGTTACTGTTAGTCACATTAAATCCACAGCCAATAAGTA
TATAAAATGTTTCTCCCATGAGTGTTGAGTTAACCAGAACTCCGCCGATCTTCATGAGGT
CACTGGTATAAATATCGTTGGGCCACTTCACTCGTAAGTTGATATCCTGATACTNCGGAA
TGGACCTCACTGCTTCCACGACAGCCACGGGACATCAGATGCTGGACAAACGGGATCCTC
TGTCCCAGCTGGGATCTCATTGGGATGGAGATGAGCANAGTNAAAAGAGCCATTCCACAG
GGCTTAACCACCATTCCCTCCCCGTCCTTTTGCCTCGGTCTGCCGGGCCGGGATCCTTAT
TAAACCATTTCTGCGGGGTTTGAAACATCACCCATCCCAGAACCCTTCTTGGGGGGGTAC
TTTGGAAAAAATATACTTTCCCACTGCTGGGCTGCATATTA
Restriction Sites NotI-NotI     
ACCN NM_000411
Insert Size 3100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000411.4, NP_000402.3
RefSeq Size 5991 bp
RefSeq ORF 2181 bp
Locus ID 3141
Cytogenetics 21q22.13
Domains BPL_C, BPL_LipA_LipB
Protein Pathways Biotin metabolism, Metabolic pathways
Gene Summary 'This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jun 2011]'
Transcript Variant: This variant (1) is the shortest transcript. Variants 1, 2 and 3 encode the same protein.

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