HLCS (NM_000411) Human Untagged Clone

CAT#: SC119915

HLCS (untagged)-Human holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (HLCS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HLCS
• Synonyms: HCS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119915 sequence for NM_000411 edited (data generated by NextGen Sequencing)
  ATGGAAGATAGACTCCACATGGATAATGGACTGGTACCCCAAAAGATTGTGTCGGTGCAC
  TTGCAGGACTCCACTCTGAAGGAAGTTAAGGATCAGGTCTCAAACAAGCAAGCCCAGATC
  CTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCTGAGCAGGACGGTATGGAGCAT
  GTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGAACCCAAACAAAGGAGAGGCAGTGCC
  TCTGGGAGTGAGCCTGCTGGGGACAGTGACAGGGGAGGGGGCCCCGTTGAGCATTATCAC
  CTCCATCTGTCTAGTTGCCACGAGTGTCTGGAACTTGAGAACAGCACCATTGAGTCAGTC
  AAGTTTGCGTCTGCCGAGAACATTCCAGACCTTCCCTACGATTATAGCAGCAGTTTGGAG
  AGTGTTGCTGATGAGACCTCCCCCGAAAGAGAAGGGAGGAGAGTCAACCTCACGGGAAAG
  GCACCCAACATCCTCCTCTATGTGGGCTCCGACTCCCAGGAAGCCCTCGGCCGGTTCCAC
  GAGGTCCGGTCTGTGCTGGCCGACTGTGTGGACATTGACAGTTATATTCTCTACCACCTG
  CTGGAGGACAGTGCTCTCAGAGACCCGTGGACGGACAACTGTCTGCTGTTGGTCATTGCT
  ACCAGGGAGTCCATTCCCGAAGACCTGTACCAGAAGTTCATGGCCTATCTTTCTCAGGGA
  GGGAAGGTGTTGGGCCTGTCTTCATCCTTCACCTTTGGTGGCTTTCAGGTGACAAGCAAG
  GGTGCACTGCACAAGACAGTCCAGAACTTGGTTTTCTCCAAGGCTGACCAGAGYGAGGTG
  AAGCTCAGCGTCTTGAGCAGTGGCTGCAGGTACCAGGAAGGCCCCGTCCGGCTCAGCCCC
  GGCAGGCTCCAGGGCCACCTGGAGAATGAGGACAAGGACAGGATGATTGTGCATGTGCCT
  TTTGGAACTCGCGGGGGAGAAGCTGTTCTTTGCCAGGTGCACTTAGAACTACCTCCCAGC
  TCCAACATAGTGCAAACTCCAGAAGATTTTAACTTGCTCAAGTCAAGCAATTTTAGAAGA
  TACGAAGTCCTTAGAGAGATTCTGACAACCCTTGGCCTCAGCTGTGACATGAAACAAGTT
  CCTGCCTTAACTCCTCTTTACTTGCTGTCAGCTGCGGAGGAAATCAGGGATCCTCTTATG
  CAGTGGCTTGGGAAACATGTGGACTCCGAGGGAGAAATAAAATCCGGCCAGCTCTCTCTT
  AGATTTGTTTCATCCTACGTGTCTGAAGTAGAAATAACCCCATCTTGTATACCTGTGGTG
  ACCAACATGGAGGCCTTCTCATCAGAACATTTCAACTTAGAGATCTATCGCCAAAATCTG
  CAGACCAAGCAGTTGGGGAAAGTAATTTTGTTTGCCGAAGTGACCCCCACAACGATGCGT
  CTCCTGGATGGGCTGATGTTTCAGACACCGCAGGAAATGGGCTTAATAGTGATCGCGGCC
  CGGCAGACCGAGGGCAAAGGACGGGGAGGGAATGTGTGGCTGAGCCCTGTGGGATGTGCT
  CTTTCTACTCTGCTCATCTCCATTCCACTGAGATCCCAGCTGGGACAGAGGATCCCGTTT
  GTCCAGCATCTGATGTCCGTGGCTGTCGTGGAAGCAGTGAGGTCCATTCCCGAGTATCAG
  GATATCAACTTACGAGTGAAGTGGCCCAACGATATTTATTACAGTGACCTCATGAAGATC
  GGCGGAGTTCTGGTTAACTCAACACTCATGGGAGAAACATTTTATATACTTATTGGCTGT
  GGATTTAATGTGACTAACAGTAACCCTACCATCTGCATCAACGACCTCATCACAGAATAC
  AATAAACAACACAAGGCAGAACTGAAGCCCTTAAGAGCCGATTATCTCATCGCCAGAGTC
  GTGACTGTGCTGGAGAAACTGATCAAAGAGTTTCAGGACAAAGGGCCCAACAGCGTCCTT
  CCCCTTTATTACCGATACTGGGTCCACAGTGGTCAGCAAGTCCATCTGGGCAGCGCAGAG
  GGACCAAAGGTGTCCATCGTTGGCCTGGACGATTCTGGCTTCCTCCAGGTTCACCAGGAG
  GGCGGCGAGGTTGTGACTGTGCACCCGGACGGCAACTCCTTCGACATGCTGAGAAACCTC
  ATCCTCCCCAAACGGCGGTAA
  
  Clone variation with respect to NM_000411.6
  834 c=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000411 unedited
  GTTCACATTCGGCACCAGGACGGGGCTGGGGCGCGGGCAGGATTTGGGGCTGCGCCGAGG
  GCGTCCCGACCTGGCCCTTTGCCACGGTAGGTTCCGGCTCCCATGCCTCCGCTGCCATCT
  GCGCTGTCCTTGGCGACTACGCCTTCACCCCCCTGGGTGCGTTTCAGCGGTCCCGGAGCC
  TGGCGGGAGCGGGGCCAGGAGCGCTCCGCGGCCGGTATCAGGCCTGCAGTCCATTGAAGA
  CTTGAACAAGTGGGCCCTATTTCTTGTGTCTCCTTTTATACTTGAAGCAGAACACATAGC
  ATTTGTGACGGAGAGCATTTGGGTACAAAGTGAGAATTTACAGAGATCATCCTCTTCAGA
  AACAGTTCGGTTTTTGCCCACTAGGGATGATGTGGTTTCTCATGAGGTTACTTGCTCTAA
  AGGACTTTATATTTTGGAACCATAAGAGCACCCTTGTGGCCCAGGCACTTTATGGATGAT
  CCCTTTTAGTGCTCCCAGTAACCTTCCAAGATTGTCAAGTGGTCAGACTGTTGTTTGCCA
  TTAGCTTGCAGACCTGGGGATCCTTATCGGCTAATTGCTGAAGCAAGTGTGGACAACTTC
  AGCAAGCTGGGGGTGGCGTTCATGGAAGATAGACTCCACATGGATAATGGACTGGTACCC
  CAAAAGATTGTGTCGGTGCACTTGCAGGACTCCACTCTGAAGGAAGTTAAGGATCAGGTC
  TCAAACAAGCAAGCCCAGATCCTAGAGCCGAAGCCTGAACCTTCTCTTGAGATTAAGCCT
  GAGCAGGACGGTATGGAGCATGTTGGCAGAGATGACCCAAAGGCTCTTGGTGAAGACCCC
  AAACCAAGGAGAGCAGTGCCTCTGGGAGTGACCTGCTGGGACAGTGACAGGGAAGGGGGC
  CCCGTTGAGCATATCACCCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000411 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACAAATGAATTGGAG
  GAAAAGAAAATTCACCTACAACTCTAAATTAGATTTCCAGATGCATGGGCACGGACAGGC
  AGCCGCGTCTCGGGGACGCCCGGCATTACCGCCGTTTGGGGAGGATGAGGTTTCTCAGCA
  TGTCGAAGGAGTTGCCGTCCGGGTGCACAGTCACAACCTCGCCGCCCTCCTGGTGAACCT
  GGAGGAAGCCAGAATCGTCCAGGCCAACGATGGACACCTTTGGTCCCTCTGCGCTGCCCA
  GATGGACTTGCTGACCACTGTGGACCCAGTATCGGTAATAAAGGGGAAGGACGCTGTTGG
  GCCCTTTGTCCTGAAACTCTTTGATCAGTTTCTCCAGCACAGTCACGACTCTGGCGATGA
  GATAATCGGCTCTTAAGGGCTTCAGTTCTGCCTTGTGTTGTTTATTGTATTCTGTGATGA
  GGTCGTTGATGCAAATGGTAGGGTTACTGTTAGTCACATTAAATCCACAGCCAATAAGTA
  TATAAAATGTTTCTCCCATGAGTGTTGAGTTAACCAGAACTCCGCCGATCTTCATGAGGT
  CACTGGTATAAATATCGTTGGGCCACTTCACTCGTAAGTTGATATCCTGATACTNCGGAA
  TGGACCTCACTGCTTCCACGACAGCCACGGGACATCAGATGCTGGACAAACGGGATCCTC
  TGTCCCAGCTGGGATCTCATTGGGATGGAGATGAGCANAGTNAAAAGAGCCATTCCACAG
  GGCTTAACCACCATTCCCTCCCCGTCCTTTTGCCTCGGTCTGCCGGGCCGGGATCCTTAT
  TAAACCATTTCTGCGGGGTTTGAAACATCACCCATCCCAGAACCCTTCTTGGGGGGGTAC
  TTTGGAAAAAATATACTTTCCCACTGCTGGGCTGCATATTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000411
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000411.4, NP_000402.3
• RefSeq Size: 5991 bp
• RefSeq ORF: 2181 bp
• Locus ID: 3141
• Cytogenetics: 21q22.13
• Domains: BPL_C, BPL_LipA_LipB
• Protein Pathways: Biotin metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jun 2011]'
  Transcript Variant: This variant (1) is the shortest transcript. Variants 1, 2 and 3 encode the same protein.