HRG (NM_000412) Human Untagged Clone

CAT#: SC119916

HRG (untagged)-Human histidine-rich glycoprotein (HRG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HRG
• Synonyms: HPRG; HRGP; THPH11
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000412, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGGCACTCATTGCAGCACTGCTTTTGATCACATTGCAGTATTCGTGTGCCGTGAGTCCCACTGACT
  GCAGTGCTGTTGAGCCGGAGGCTGAGAAAGCTCTAGACCTGATCAATAAAAGGCGACGGGATGGCTACCT
  TTTCCAATTGCTGCGGATTGCTGATGCCCACTTGGACAGAGTGGAAAATACAACTGTATATTACTTAGTC
  TTAGATGTGCAAGAATCGGACTGTTCGGTCCTATCCAGGAAATACTGGAATGACTGTGAGCCACCTGATT
  CCAGACGTCCATCTGAAATAGTGATCGGACAATGTAAGGTAATAGCTACAAGACATTCCCATGAATCTCA
  GGACCTCAGAGTGATTGACTTTAACTGCACCACAAGTTCTGTCTCTTCAGCACTGGCCAATACCAAAGAT
  AGTCCGGTCCTCATAGATTTCTTTGAGGATACTGAGCGCTACAGAAAACAAGCCAACAAAGCCCTTGAGA
  AGTACAAAGAGGAGAATGATGACTTTGCCTCTTTCAGAGTGGACCGAATCGAGAGAGTTGCAAGAGTGAG
  AGGAGGGGAAGGAACTGGTTACTTCGTGGACTTCTCTGTGCGGAACTGCCCCAGACACCATTTCCCCAGA
  CACCCCAATGTCTTTGGATTCTGCAGAGCAGATTTGTTCTATGATGTAGAAGCCTTGGACTTGGAAAGCC
  CGAAAAACCTTGTCATAAACTGTGAAGTCTTCGACCCTCAGGAACATGAGAACATCAATGGTGTACCGCC
  TCATTTGGGACATCCCTTCCACTGGGGTGGGCATGAGCGTTCTTCTACCACCAAGCCTCCATTCAAGCCC
  CATGGATCTAGAGATCATCATCATCCCCACAAGCCACACGAACATGGACCCCCACCTCCTCCAGATGAAA
  GAGATCACTCACATGGACCCCCACTTCCACAAGGCCCTCCTCCACTATTGCCCATGTCCTGCTCAAGTTG
  TCAACATGCCACTTTTGGCACAAATGGGGCCCAAAGACATTCTCATAATAATAATTCCAGTGACCTCCAT
  CCCCATAAGCATCATTCCCATGAACAGCATCCCCACGGACACCATCCCCATGCACACCATCCTCATGAAC
  ATGATACCCATAGACAGCATCCCCATGGACACCACCCCCATGGACACCATCCTCATGGACACCACCCCCA
  TGGACACCATCCCCATGGACACCATCCCCACTGCCATGATTTCCAAGACTATGGACCTTGTGACCCACCA
  CCCCATAACCAAGGTCACTGTTGCCATGGCCACGGCCCACCACCTGGGCACTTAAGAAGGCGAGGCCCAG
  GTAAAGGACCCCGTCCCTTCCATTGCAGACAAATTGGATCTGTGTACCGACTCCCTCCTCTAAGAAAAGG
  TGAGGTGCTGCCACTTCCTGAGGCCAATTTTCCCAGCTTCCCATTGCCGCACCACAAACATCCTCTAAAG
  CCAGACAATCAGCCCTTTCCTCAATCAGTCTCTGAATCATGTCCAGGGAAGTTCAAGAGTGGGTTTCCAC
  AAGTTTCCATGTTTTTTACACATACATTTCCAAAATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000412 unedited
  AAAGTTCACATTTGTATACGACTCATATAGGCGGCCGCGCAATTCGCACGAGAATGAAGG
  CACTCATTGCAGCACTGCTTTTGATCACATTGCAGTATTCGTGTGCCGTGAGTCCCACTG
  ACTGCAGTGCTGTTGAGCCGGAGGCTGAGAAAGCTCTACACCTGATCAATAAAAGGCGAC
  GGGATGGCTACCTTTTCCAATTGCTGCGGATTGCTGATGCCCACTTGGACAGAGTGGAAA
  ATACAACTGTATATTACTTACTCTTAAATGTGCAAGAATCGGACTGTTCGGTCCTATCCA
  GGAAATACTGGAATGACTGTGAGCCACCTGATTCCAGACGTCCATCTGAAATAGTGATCG
  GACAATGTAAGGTAATAGCTACAAGACATTCCCATGAATCTCATGACCTCACAGTGATTG
  ACTTTAACTGCACCACAAGTTCTGTCTCTTCAACACTGGCCAATACCAAAGATAGTCCGG
  TCCTCATAGATTTCTTTGAGGATACTGAGCGCTACAGAAAACAAGCCAACAAAGCCCTTG
  AGAAGTACAAAGAGGACAATGATGACTTTGCCTCTTTCAGAGTGGACCGAATCGAGAGAG
  TTGCAAGAGTGAGAGGAGGGCGAAGGAACTGGTTACTTCGTGGACTTCTCTGTGCGGAAC
  TGCCCCAGACACCATTTCCCCAGACACCCCAATGTCTTTGGATTCTGCAGAGCATATTTG
  TTCTATGATGTAAAAGCCTTGGACTTGGTAAGCCCGACAAACTTGTCATAAACTGTGAAG
  TCTTCGACCCTCAAGAACATGAGAACTCAATGGTGTACCGCCTCATTTGGGACATCCCTT
  ACCTGGTGGTGGGCATGAACGTTCTTCTACCACCAACCTCCTTTCAGCCCCATGGATCTT
  GAGATCATCATCATCCGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000412
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000412.2, NP_000403.1
• RefSeq Size: 1964 bp
• RefSeq ORF: 1578 bp
• Locus ID: 3273
• Cytogenetics: 3q27.3
• Domains: CY
• Protein Families: Secreted Protein
• Gene Summary: 'This histidine-rich glycoprotein contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. The encoded protein also has a peptide that displays antimicrobial activity against C. albicans, E. coli, S. aureus, P. aeruginosa, and E. faecalis. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. [provided by RefSeq, Nov 2014]'