SRD5A2 (NM_000348) Human Untagged Clone

CAT#: SC119922

SRD5A2 (untagged)-Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SRD5A2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000348 edited
  CGGCACGAGGCAGCGGCCACCGGCGAGGAACACGGCGCGATGCAGGTTCAGTGCCAGCAG
  AGCCCAGTGCTGGCAGGCAGCGCCACTTTGGTCGCCCTTGGGGCACTGGCCTTGTACGTC
  GCGAAGCCCTCCGGCTACGGGAAGCACACGGAGAGCCTGAAGCCGGCGGCTACCCGCCTG
  CCAGCCCGCGCCGCCTGGTTCCTGCAGGAGCTGCCTTCCTTCGCGGTGCCCGCGGGGATC
  CTCGCCCGGCAGCCCCTCTCCCTCTTCGGGCCACCTGGGACGGTACTTCTGGGCCTCTTC
  TGCCTACATTACTTCCACAGGACATTTGTGTACTCACTGCTCAATCGAGGGAGGCCTTAT
  CCAGCTATACTCATTCTCAGAGGCACTGCCTTCTGCACTGGAAATGGAGTCCTTCAAGGC
  TACTATCTGATTTACTGTGCTGAATACCCTGATGGGTGGTACACAGACATACGGTTTAGC
  TTGGGTGTCTTCTTATTTATTTTGGGAATGGGAATAAACATTCATAGTGACTATATATTG
  CGCCAGCTCAGGAAGCCTGGAGAAATCAGCTACAGGATTCCACAAGGTGGCTTGTTTACG
  TATGTTTCTGGAGCCAATTTCCTCGGTGAGATCATTGAATGGATCGGCTATGCCCTGGCC
  ACTTGGTCCCTCCCAGCACTTGCATTTGCATTTTTCTCACTTTGTTTCCTTGGGCTGCGA
  GCTTTTCACCACCATAGGTTCTACCTCAAGATGTTTGAGGACTACCCCAAATCTCGGAAA
  GCCCTTATTCCATTCATCTTTTAAAGGAACCAAATTAAAAAGGAGCAGAGCTCCCACAAT
  GCTGATGAAAACTGTCAAGCTGCTGAAACTGTAATTTTCATGATATAATAGTCCCGTATA
  TATGTAATAGTAGGTCTCCTGGCGTTCTGCCAGCTGGCCTGGGGATTCTGAGTGGTGTCT
  GCTTAGAGTTTACTCCTACCCTTCCAGGGACCCCTATCCTGATCCCCAACTGAAGCTTCA
  AAAAGCCACTTTTCCAAATGGCGACAGTTGCTTCTTAGCTATTGCTCTGAGAAAGTACAA
  ACTTCTCCTATGTCTTTCACCGGGCAATCCAAGTACATGTGGCTTCATACCCACTCCCTG
  TCAATGCAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000348 unedited
  TCGCATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCAGCGGCC
  ACCGGCGAGGAACACGGCGCGATGCAGGTTCAGTGCCAGCAGAGCCCAGTGCTGGCAGGC
  AGCGCCACTTTGGTCGCCCTTGGGGCACTGGCCTTGTACGTCGCGAAGCCCTCCGGCTAC
  GGGAAGCACACGGAGAGCCTGAAGCCGGCGGCTACCCGCCTGCCAGCCCGCGCCGCCTGG
  TTCCTGCAGGAGCTGCCTTCCTTCGCGGTGCCCGCGGGGATCCTCGCCCGGCAGCCCCTC
  TCCCTCTTCGGGCCACCTGGGACGGTACTTCTGGGCCTCTTCTGCCTACATTACTTCCAC
  AGGACATTTGTGTACTCACTGCTCAATCGAGGGAGGCCTTATCCAGCTATACTCATTCTC
  AGAGGCACTGCCTTCTGCACTGGAAATGGAGTCCTTCAAGGCTACTATCTGATTTACTGT
  GCTGAATACCCTGATGGGTGGTACACAGACATACGGTTTAGCTTGGGTGTCTTCTTATTT
  ATTTTGGGAATGGGAATAAACATTCATAGTGACTATATATTGCGCCAGCTCAGGAAGCCT
  GGAGAAATCAGCTACAGGATTCCACAAGGTGGCTTGTTTACGTATGTTTCTGGAGCCAAT
  TTCCTCGGTGAGATCATTGAATGGNATCGCTATGCCCTGGCCACNTGGTCCCTCCNAGCA
  CTTGCATTTGCATNTTTCTCACTTTTGTTTCTTGGGCTGCGAGCTTTTCACCACCATAAG
  TTCTACCTCAAGATGTTGAGGGACTACCCAATCTCGAAAGCCCCTATNCATTCATCTTTA
  AGGAACCANTTAAAAAGAGCAGACTCCNACATGCTGATGAAACTGTCAGCTGCTGAACTG
  GNATTTCATGAATATAGTCCGTTATATGTAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000348 unedited
  GCCGCAATCTAGAGTCGAGNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGGA
  TTAAAAGGTTTTTAATGAACAACAAAAACACTTATTTATATGATGGCAATTGGCATTTTT
  CAGTAATCTACATCATCCTAAAACCTTTAAAGTTTCCTATGGGACTTATATAGGGAGTTT
  CTGGGCTTAGTCCCACTGGGGCTCATTTGTCAAAACAGTTGGCTAAATATTGACAATGCA
  TTCCGCAAACATAGGCCTGAAAAAACAAATATTTCCATGTATTCCAATTACAAGCGTTCG
  GCCCCTTCCTTAAAGAGTCCATATCTCAGCACTCATGCTGGGGGGACCCCTTCACAAAAG
  TTGGCAAACTCAAAGGCTTACTACCTACGTAGTTCCAGTTCTGGGGTGGAAATGTGGGCT
  CTGTATGTTCAAATCCTCCAACTTTTTAAAGGAACCTAAAATGCTAGAGTTTTATGAAGG
  AATCTCCTGATTTTAAAGTTTTTGAACCTTTGGAAAAAAAAAAAAACCTTTTGGTCTGAC
  CCAAACCAACCAGGTTTTTTGCCAAAACGTGCCCCTTTGTGTGCAATTTTCAGCCTCTGC
  CTGCAGGTCCTTTGAGGAAGGCATTCAGGCCGCCCTTAGGAAACACCTTCTTGACACGGC
  CCGAAAATTCAAGGAAAACCTCCTTAAAGGAAATTTTTTTGGGGCTCTCGCGTCCCTCAT
  TTGGCGGGGACCTCGGGGGGGGGCAACACCCCTGCCACCCTATCATATACCCTCCCGCCC
  TACCCCCACCATTTTCGGCCTCCGCCCCCCCCCCCCACACACCTACGTTCCCCCCGCCTT
  TGCAAATTGTTTCCTTCCCCGCGGACCCCCACAGCCATTCGCGTCCCTCCCTTTTCCACA
  CACCCCACGTTAGTCCTTTTTGTCTCCGCGTCCATATATTGCCCGTCCAACTATCCGTCA
  CTATTATTGGCCTCCGGTCCCCCCCTGATTACATATTCTCTTATCACAACCTGCGTCGCC
  CTCACGAGGGTCGTTGTTGTCGCTTAGCCAACTCCATTTAGGTGCTACTGATAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000348
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000348.2, NP_000339.2
• RefSeq Size: 2464 bp
• RefSeq ORF: 765 bp
• Locus ID: 6716
• Cytogenetics: 2p23.1
• Domains: Steroid_dh
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Prostate cancer
• Gene Summary: 'This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). [provided by RefSeq, Jul 2008]'