SRD5A2 (NM_000348) Human Untagged Clone

CAT#: SC119922

SRD5A2 (untagged)-Human steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) (SRD5A2)


  "NM_000348" in other vectors (6)

Reconstitution Protocol

USD 420.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SRD5A2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_000348 edited
CGGCACGAGGCAGCGGCCACCGGCGAGGAACACGGCGCGATGCAGGTTCAGTGCCAGCAG
AGCCCAGTGCTGGCAGGCAGCGCCACTTTGGTCGCCCTTGGGGCACTGGCCTTGTACGTC
GCGAAGCCCTCCGGCTACGGGAAGCACACGGAGAGCCTGAAGCCGGCGGCTACCCGCCTG
CCAGCCCGCGCCGCCTGGTTCCTGCAGGAGCTGCCTTCCTTCGCGGTGCCCGCGGGGATC
CTCGCCCGGCAGCCCCTCTCCCTCTTCGGGCCACCTGGGACGGTACTTCTGGGCCTCTTC
TGCCTACATTACTTCCACAGGACATTTGTGTACTCACTGCTCAATCGAGGGAGGCCTTAT
CCAGCTATACTCATTCTCAGAGGCACTGCCTTCTGCACTGGAAATGGAGTCCTTCAAGGC
TACTATCTGATTTACTGTGCTGAATACCCTGATGGGTGGTACACAGACATACGGTTTAGC
TTGGGTGTCTTCTTATTTATTTTGGGAATGGGAATAAACATTCATAGTGACTATATATTG
CGCCAGCTCAGGAAGCCTGGAGAAATCAGCTACAGGATTCCACAAGGTGGCTTGTTTACG
TATGTTTCTGGAGCCAATTTCCTCGGTGAGATCATTGAATGGATCGGCTATGCCCTGGCC
ACTTGGTCCCTCCCAGCACTTGCATTTGCATTTTTCTCACTTTGTTTCCTTGGGCTGCGA
GCTTTTCACCACCATAGGTTCTACCTCAAGATGTTTGAGGACTACCCCAAATCTCGGAAA
GCCCTTATTCCATTCATCTTTTAAAGGAACCAAATTAAAAAGGAGCAGAGCTCCCACAAT
GCTGATGAAAACTGTCAAGCTGCTGAAACTGTAATTTTCATGATATAATAGTCCCGTATA
TATGTAATAGTAGGTCTCCTGGCGTTCTGCCAGCTGGCCTGGGGATTCTGAGTGGTGTCT
GCTTAGAGTTTACTCCTACCCTTCCAGGGACCCCTATCCTGATCCCCAACTGAAGCTTCA
AAAAGCCACTTTTCCAAATGGCGACAGTTGCTTCTTAGCTATTGCTCTGAGAAAGTACAA
ACTTCTCCTATGTCTTTCACCGGGCAATCCAAGTACATGTGGCTTCATACCCACTCCCTG
TCAATGCAA
>OriGene 5' read for NM_000348 unedited
TCGCATTTTGTAATACGACTCACTATAGGGCGGCNCGCGAATTCGCACGAGGCAGCGGCC
ACCGGCGAGGAACACGGCGCGATGCAGGTTCAGTGCCAGCAGAGCCCAGTGCTGGCAGGC
AGCGCCACTTTGGTCGCCCTTGGGGCACTGGCCTTGTACGTCGCGAAGCCCTCCGGCTAC
GGGAAGCACACGGAGAGCCTGAAGCCGGCGGCTACCCGCCTGCCAGCCCGCGCCGCCTGG
TTCCTGCAGGAGCTGCCTTCCTTCGCGGTGCCCGCGGGGATCCTCGCCCGGCAGCCCCTC
TCCCTCTTCGGGCCACCTGGGACGGTACTTCTGGGCCTCTTCTGCCTACATTACTTCCAC
AGGACATTTGTGTACTCACTGCTCAATCGAGGGAGGCCTTATCCAGCTATACTCATTCTC
AGAGGCACTGCCTTCTGCACTGGAAATGGAGTCCTTCAAGGCTACTATCTGATTTACTGT
GCTGAATACCCTGATGGGTGGTACACAGACATACGGTTTAGCTTGGGTGTCTTCTTATTT
ATTTTGGGAATGGGAATAAACATTCATAGTGACTATATATTGCGCCAGCTCAGGAAGCCT
GGAGAAATCAGCTACAGGATTCCACAAGGTGGCTTGTTTACGTATGTTTCTGGAGCCAAT
TTCCTCGGTGAGATCATTGAATGGNATCGCTATGCCCTGGCCACNTGGTCCCTCCNAGCA
CTTGCATTTGCATNTTTCTCACTTTTGTTTCTTGGGCTGCGAGCTTTTCACCACCATAAG
TTCTACCTCAAGATGTTGAGGGACTACCCAATCTCGAAAGCCCCTATNCATTCATCTTTA
AGGAACCANTTAAAAAGAGCAGACTCCNACATGCTGATGAAACTGTCAGCTGCTGAACTG
GNATTTCATGAATATAGTCCGTTATATGTAAC
>OriGene 3' read for NM_000348 unedited
GCCGCAATCTAGAGTCGAGNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGGA
TTAAAAGGTTTTTAATGAACAACAAAAACACTTATTTATATGATGGCAATTGGCATTTTT
CAGTAATCTACATCATCCTAAAACCTTTAAAGTTTCCTATGGGACTTATATAGGGAGTTT
CTGGGCTTAGTCCCACTGGGGCTCATTTGTCAAAACAGTTGGCTAAATATTGACAATGCA
TTCCGCAAACATAGGCCTGAAAAAACAAATATTTCCATGTATTCCAATTACAAGCGTTCG
GCCCCTTCCTTAAAGAGTCCATATCTCAGCACTCATGCTGGGGGGACCCCTTCACAAAAG
TTGGCAAACTCAAAGGCTTACTACCTACGTAGTTCCAGTTCTGGGGTGGAAATGTGGGCT
CTGTATGTTCAAATCCTCCAACTTTTTAAAGGAACCTAAAATGCTAGAGTTTTATGAAGG
AATCTCCTGATTTTAAAGTTTTTGAACCTTTGGAAAAAAAAAAAAACCTTTTGGTCTGAC
CCAAACCAACCAGGTTTTTTGCCAAAACGTGCCCCTTTGTGTGCAATTTTCAGCCTCTGC
CTGCAGGTCCTTTGAGGAAGGCATTCAGGCCGCCCTTAGGAAACACCTTCTTGACACGGC
CCGAAAATTCAAGGAAAACCTCCTTAAAGGAAATTTTTTTGGGGCTCTCGCGTCCCTCAT
TTGGCGGGGACCTCGGGGGGGGGCAACACCCCTGCCACCCTATCATATACCCTCCCGCCC
TACCCCCACCATTTTCGGCCTCCGCCCCCCCCCCCCACACACCTACGTTCCCCCCGCCTT
TGCAAATTGTTTCCTTCCCCGCGGACCCCCACAGCCATTCGCGTCCCTCCCTTTTCCACA
CACCCCACGTTAGTCCTTTTTGTCTCCGCGTCCATATATTGCCCGTCCAACTATCCGTCA
CTATTATTGGCCTCCGGTCCCCCCCTGATTACATATTCTCTTATCACAACCTGCGTCGCC
CTCACGAGGGTCGTTGTTGTCGCTTAGCCAACTCCATTTAGGTGCTACTGATAAA
Restriction Sites NotI-NotI     
ACCN NM_000348
Insert Size 2550 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000348.2, NP_000339.2
RefSeq Size 2464 bp
RefSeq ORF 765 bp
Locus ID 6716
Cytogenetics 2p23.1
Domains Steroid_dh
Protein Families Druggable Genome, Transmembrane
Protein Pathways Androgen and estrogen metabolism, Prostate cancer
Gene Summary 'This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). [provided by RefSeq, Jul 2008]'

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