TGFBI (NM_000358) Human Untagged Clone

CAT#: SC119927

TGFBI (untagged)-Human transforming growth factor, beta-induced, 68kDa (TGFBI)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TGFBI
• Synonyms: BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; LCD1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119927 sequence for NM_000358 edited (data generated by NextGen Sequencing)
  ATGGCGCTCTTCGTGCGGCTGCTGGCTCTCGCCCTGGCTCTGGCCCTGGGCCCCGCCGCG
  ACCCTGGCGGGTCCCGCCAAGTCGCCCTACCAGCTGGTGCTGCAGCACAGCAGGCTCCGG
  GGCCGCCAGCACGGCCCCAACGTGTGTGCTGTGCAGAAGGTTATTGGCACTAATAGGAAG
  TACTTCACCAACTGCAAGCAGTGGTACCAAAGGAAAATCTGTGGCAAATCAACAGTCATC
  AGCTACGAGTGCTGTCCTGGATATGAAAAGGTCCCTGGGGAGAAGGGCTGTCCAGCAGCC
  CTACCACTCTCAAACCTTTACGAGACCCTGGGAGTCGTTGGATCCACCACCACTCAGCTG
  TACACGGACCGCACGGAGAAGCTGAGGCCTGAGATGGAGGGGCCCGGCAGCTTCACCATC
  TTCGCCCCTAGCAACGAGGCCTGGGCCTCCTTGCCAGCTGAAGTGCTGGACTCCCTGGTC
  AGCAATGTCAACATTGAGCTGCTCAATGCCCTCCGCTACCATATGGTGGGCAGGCGAGTC
  CTGACTGATGAGCTGAAACACGGCATGACCCTCACCTCTATGTACCAGAATTCCAACATC
  CAGATCCACCACTATCCTAATGGGATTGTAACTGTGAACTGTGCCCGGCTCCTGAAAGCC
  GACCACCATGCAACCAACGGGGTGGTGCACCTCATCGATAAGGTCATCTCCACCATCACC
  AACAACATCCAGCAGATCATTGAGATCGAGGACACCTTTGAGACCCTTCGGGCTGCTGTG
  GCTGCATCAGGGCTCAACACGATGCTTGAAGGTAACGGCCAGTACACGCTTTTGGCCCCG
  ACCAATGAGGCCTTCGAGAAGATCCCTAGTGAGACTTTGAACCGTATCCTGGGCGACCCA
  GAAGCCCTGAGAGACCTGCTGAACAACCACATCTTGAAGTCAGCTATGTGTGCTGAAGCC
  ATCGTTGCGGGGCTGTCTGTAGAGACCCTGGAGGGCACGACACTGGAGGTGGGCTGCAGC
  GGGGACATGCTCACTATCAACGGGAAGGCGATCATCTCCAATAAAGACATCCTAGCCACC
  AACGGGGTGATCCACTACATTGATGAGCTACTCATCCCAGACTCAGCCAAGACACTATTT
  GAATTGGCTGCAGAGTCTGATGTGTCCACAGCCATTGACCTTTTCAGACAAGCCGGCCTC
  GGCAATCATCTCTCTGGAAGTGAGCGGTTGACCCTCCTGGCTCCCCTGAATTCTGTATTC
  AAAGATGGAACCCCTCCAATTGATGCCCATACAAGGAATTTGCTTCGGAACCACATAATT
  AAAGACCAGCTGGCCTCTAAGTATCTGTACCATGGACAGACCCTGGAAACTCTGGGCGGC
  AAAAAACTGAGAGTTTTTGTTTATCGTAATAGCCTCTGCATTGAGAACAGCTGCATCGCG
  GCCCACGACAAGAGGGGGAGGTACGGGACCCTGTTCACGATGGACCGGGTGCTGACCCCC
  CCAATGGGGACTGTCATGGATGTCCTGAAGGGAGACAATCGCTTTAGCATGCTGGTAGCT
  GCCATCCAGTCTGCAGGACTGACGGAGACCCTCAACCGGGAAGGAGTCTACACAGTCTTT
  GCTCCCACAAATGAAGCCTTCCGAGCCCTGCCACCAAGAGAACGGAGCAGACTCTTGGGA
  GATGCCAAGGAACTTGCCAACATCCTGAAATACCACATTGGTGATGAAATCCTGGTTAGC
  GGAGGCATCGGGGCCCTGGTGCGGCTAAAGTCTCTCCAAGGTGACAAGCTGGAAGTCAGC
  TTGAAAAACAATGTGGTGAGTGTCAACAAGGAGCCTGTTGCCGAGCCTGACATCATGGCC
  ACAAATGGCGTGGTCCATGTCATCACCAATGTTCTGCAGCCTCCAGCCAACAGACCTCAG
  GAAAGAGGGGATGAACTTGCAGACTCTGCGCTTGAGATCTTCAAACAAGCATCAGCGTTT
  TCCAGGGCTTCCCAGAGGTCTGTGCGACTAGCCCCTGTCTATCAAAAGTTATTAGAGAGG
  ATGAAGCATTAG
  
  Clone variation with respect to NM_000358.2
  651 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000358 unedited
  ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGCCGCCCGCT
  TGCCCGTCGGTCGCTAGCTCGCTCGGTGCGCGTCGTCCCGCTCCATGGCGCTCTTCGTGC
  GGCTGCTGGCTCTCGCCCTGGCTCTGGCCCTGGGCCCCGCCGCGACCCTGGCGGGTCCCG
  CCAAGTCGCCCTACCAGCTGGTGCTGCAGCACAGCAGGCTCCGGGGCCGCCAGCACGGCC
  CCAACGTGTGTGCTGTGCAGAAGGTTATTGGCACTAATAGGAAGTACTTCACCAACTGCA
  AGCAGTGGTACCAAAGGAAAATCTGTGGCAAATCAACAGTCATCAGCTACGAGTGCTGTC
  CTGGATATGAAAAGGTCCCTGGGNAGAAAGGGCTGTCCAGCAGCCCTACCACTCTCAAAC
  CTTTACGAGACCCTGGGAGTCGTTGGATCCACCACCACTCAGCTGTACACGGACCGCACG
  GAGAAGCTGAGGCCTGAGATGGAGGGGCCCGGCAGCTTCACCATCTTCGCCCCTAGCAAC
  GAGGCCTGGGCCTCCTTGCCAGCTGAAGTGCTGGACTCCCTGGTCAGCAATGTCAACATT
  GAGCTGCTCAATGCCCTCCGCTACCATATGGTGGGCAGGCGAGTCCTGACTGATGAGCTG
  NAACACGGCATGACCCTCACCTCTATGTACCAGAATTCCACATCCAGATCCACCACTATC
  CTAATGGGGATTGTAACCTGTGAACTGTGCCCGGCTCCTGAAAGCCGACCACCATGCACC
  AACGGGGGGTGGGGCACCTCAATCGATAAGGTCATTCTCCCCATCACCAACAACATCCAG
  CAGATAATTTGGGAACCGAGGACACCCTTGAGACCCTTCGGCCTGCTGTGGCTGGATCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000358 unedited
  GTCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTATGTTTCTTTGGTTT
  TATTATTACAAACACACCATGGCTCTGTCACAATAGGGAAATCTAAGCTCTACATACCAT
  TTCTTTTCTACCTGGACAGGGCCCCATAAAAGCCTCCAAGCCACGTGTAGATGTGAGACA
  CATTTGACAGAACATTTCAACTCATAGCTTATAATGATGCCATTTCTCCAGCTGTGCAAG
  GGCTTTACAAAAACTGTGCCAGGACTTCCCATGAGGCTGGATTGCTTGATTCATGTTTTA
  TGAGCCCCACAATACTGAAGCTCCTTTTCCAGGGACTTGGCATAGGCAGTCAATTCCACA
  TTTGGGATAGGTCCTCTCTGGAAGTGAATGTCAGGCAGTGACATCCAAGTTTCTGCATGC
  AGTGGGTTAACAGCCATGTTTAGGGGGAACATGATTTAAAAAGTACATCTCTCTCCCTCC
  TCCCCCACATGCACAAGGCTCACATCTCATTATGGTGCGGCCCATGTACATTAAAGTGTG
  ATACTTGGTTTTGAAACATTCAAACAGTCTCTGTGGAAATCTGACAGAAAATGGCGGAAA
  GCTGCCGCGGTGCATTCCTCCTGTAGTGCCTTACACCCAATGCTTCATCCTTTCTAATCC
  CTTCTGAAAACCGGGGGCCCTCCCCCAGACCTTTGGAAACCCCTGGAAAACGCTGATGCC
  TGGTCTCAGAACTCAACCCCATACTTGGAAGTTTATCCCCTTCTCCTGAGGTCCCGTGGT
  CGGTGGCTCCACACATTCGCCAGACCGGTCCCGCCCTCTTTGTGTCTCACGTCCATGTTA
  CCACCTCCCCCTATTACCCTACCCACTGCTTAAAACGCATTCCATCTGCACTCTTCCAAA
  TTTACCCCCACAGTGCCAGTTTTCCTCTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000358
• Insert Size: 2800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000358.1, NP_000349.1
• RefSeq Size: 2691 bp
• RefSeq ORF: 2052 bp
• Locus ID: 7045
• Cytogenetics: 5q31.1
• Domains: FAS1
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: 'This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]'