TGFBI (NM_000358) Human Untagged Clone

CAT#: SC119927

TGFBI (untagged)-Human transforming growth factor, beta-induced, 68kDa (TGFBI)


  "NM_000358" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TGFBI
Synonyms BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; LCD1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119927 sequence for NM_000358 edited (data generated by NextGen Sequencing)
ATGGCGCTCTTCGTGCGGCTGCTGGCTCTCGCCCTGGCTCTGGCCCTGGGCCCCGCCGCG
ACCCTGGCGGGTCCCGCCAAGTCGCCCTACCAGCTGGTGCTGCAGCACAGCAGGCTCCGG
GGCCGCCAGCACGGCCCCAACGTGTGTGCTGTGCAGAAGGTTATTGGCACTAATAGGAAG
TACTTCACCAACTGCAAGCAGTGGTACCAAAGGAAAATCTGTGGCAAATCAACAGTCATC
AGCTACGAGTGCTGTCCTGGATATGAAAAGGTCCCTGGGGAGAAGGGCTGTCCAGCAGCC
CTACCACTCTCAAACCTTTACGAGACCCTGGGAGTCGTTGGATCCACCACCACTCAGCTG
TACACGGACCGCACGGAGAAGCTGAGGCCTGAGATGGAGGGGCCCGGCAGCTTCACCATC
TTCGCCCCTAGCAACGAGGCCTGGGCCTCCTTGCCAGCTGAAGTGCTGGACTCCCTGGTC
AGCAATGTCAACATTGAGCTGCTCAATGCCCTCCGCTACCATATGGTGGGCAGGCGAGTC
CTGACTGATGAGCTGAAACACGGCATGACCCTCACCTCTATGTACCAGAATTCCAACATC
CAGATCCACCACTATCCTAATGGGATTGTAACTGTGAACTGTGCCCGGCTCCTGAAAGCC
GACCACCATGCAACCAACGGGGTGGTGCACCTCATCGATAAGGTCATCTCCACCATCACC
AACAACATCCAGCAGATCATTGAGATCGAGGACACCTTTGAGACCCTTCGGGCTGCTGTG
GCTGCATCAGGGCTCAACACGATGCTTGAAGGTAACGGCCAGTACACGCTTTTGGCCCCG
ACCAATGAGGCCTTCGAGAAGATCCCTAGTGAGACTTTGAACCGTATCCTGGGCGACCCA
GAAGCCCTGAGAGACCTGCTGAACAACCACATCTTGAAGTCAGCTATGTGTGCTGAAGCC
ATCGTTGCGGGGCTGTCTGTAGAGACCCTGGAGGGCACGACACTGGAGGTGGGCTGCAGC
GGGGACATGCTCACTATCAACGGGAAGGCGATCATCTCCAATAAAGACATCCTAGCCACC
AACGGGGTGATCCACTACATTGATGAGCTACTCATCCCAGACTCAGCCAAGACACTATTT
GAATTGGCTGCAGAGTCTGATGTGTCCACAGCCATTGACCTTTTCAGACAAGCCGGCCTC
GGCAATCATCTCTCTGGAAGTGAGCGGTTGACCCTCCTGGCTCCCCTGAATTCTGTATTC
AAAGATGGAACCCCTCCAATTGATGCCCATACAAGGAATTTGCTTCGGAACCACATAATT
AAAGACCAGCTGGCCTCTAAGTATCTGTACCATGGACAGACCCTGGAAACTCTGGGCGGC
AAAAAACTGAGAGTTTTTGTTTATCGTAATAGCCTCTGCATTGAGAACAGCTGCATCGCG
GCCCACGACAAGAGGGGGAGGTACGGGACCCTGTTCACGATGGACCGGGTGCTGACCCCC
CCAATGGGGACTGTCATGGATGTCCTGAAGGGAGACAATCGCTTTAGCATGCTGGTAGCT
GCCATCCAGTCTGCAGGACTGACGGAGACCCTCAACCGGGAAGGAGTCTACACAGTCTTT
GCTCCCACAAATGAAGCCTTCCGAGCCCTGCCACCAAGAGAACGGAGCAGACTCTTGGGA
GATGCCAAGGAACTTGCCAACATCCTGAAATACCACATTGGTGATGAAATCCTGGTTAGC
GGAGGCATCGGGGCCCTGGTGCGGCTAAAGTCTCTCCAAGGTGACAAGCTGGAAGTCAGC
TTGAAAAACAATGTGGTGAGTGTCAACAAGGAGCCTGTTGCCGAGCCTGACATCATGGCC
ACAAATGGCGTGGTCCATGTCATCACCAATGTTCTGCAGCCTCCAGCCAACAGACCTCAG
GAAAGAGGGGATGAACTTGCAGACTCTGCGCTTGAGATCTTCAAACAAGCATCAGCGTTT
TCCAGGGCTTCCCAGAGGTCTGTGCGACTAGCCCCTGTCTATCAAAAGTTATTAGAGAGG
ATGAAGCATTAG

Clone variation with respect to NM_000358.2
651 g=>c
>OriGene 5' read for NM_000358 unedited
ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAGCCGCCCGCT
TGCCCGTCGGTCGCTAGCTCGCTCGGTGCGCGTCGTCCCGCTCCATGGCGCTCTTCGTGC
GGCTGCTGGCTCTCGCCCTGGCTCTGGCCCTGGGCCCCGCCGCGACCCTGGCGGGTCCCG
CCAAGTCGCCCTACCAGCTGGTGCTGCAGCACAGCAGGCTCCGGGGCCGCCAGCACGGCC
CCAACGTGTGTGCTGTGCAGAAGGTTATTGGCACTAATAGGAAGTACTTCACCAACTGCA
AGCAGTGGTACCAAAGGAAAATCTGTGGCAAATCAACAGTCATCAGCTACGAGTGCTGTC
CTGGATATGAAAAGGTCCCTGGGNAGAAAGGGCTGTCCAGCAGCCCTACCACTCTCAAAC
CTTTACGAGACCCTGGGAGTCGTTGGATCCACCACCACTCAGCTGTACACGGACCGCACG
GAGAAGCTGAGGCCTGAGATGGAGGGGCCCGGCAGCTTCACCATCTTCGCCCCTAGCAAC
GAGGCCTGGGCCTCCTTGCCAGCTGAAGTGCTGGACTCCCTGGTCAGCAATGTCAACATT
GAGCTGCTCAATGCCCTCCGCTACCATATGGTGGGCAGGCGAGTCCTGACTGATGAGCTG
NAACACGGCATGACCCTCACCTCTATGTACCAGAATTCCACATCCAGATCCACCACTATC
CTAATGGGGATTGTAACCTGTGAACTGTGCCCGGCTCCTGAAAGCCGACCACCATGCACC
AACGGGGGGTGGGGCACCTCAATCGATAAGGTCATTCTCCCCATCACCAACAACATCCAG
CAGATAATTTGGGAACCGAGGACACCCTTGAGACCCTTCGGCCTGCTGTGGCTGGATCA
>OriGene 3' read for NM_000358 unedited
GTCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTATGTTTCTTTGGTTT
TATTATTACAAACACACCATGGCTCTGTCACAATAGGGAAATCTAAGCTCTACATACCAT
TTCTTTTCTACCTGGACAGGGCCCCATAAAAGCCTCCAAGCCACGTGTAGATGTGAGACA
CATTTGACAGAACATTTCAACTCATAGCTTATAATGATGCCATTTCTCCAGCTGTGCAAG
GGCTTTACAAAAACTGTGCCAGGACTTCCCATGAGGCTGGATTGCTTGATTCATGTTTTA
TGAGCCCCACAATACTGAAGCTCCTTTTCCAGGGACTTGGCATAGGCAGTCAATTCCACA
TTTGGGATAGGTCCTCTCTGGAAGTGAATGTCAGGCAGTGACATCCAAGTTTCTGCATGC
AGTGGGTTAACAGCCATGTTTAGGGGGAACATGATTTAAAAAGTACATCTCTCTCCCTCC
TCCCCCACATGCACAAGGCTCACATCTCATTATGGTGCGGCCCATGTACATTAAAGTGTG
ATACTTGGTTTTGAAACATTCAAACAGTCTCTGTGGAAATCTGACAGAAAATGGCGGAAA
GCTGCCGCGGTGCATTCCTCCTGTAGTGCCTTACACCCAATGCTTCATCCTTTCTAATCC
CTTCTGAAAACCGGGGGCCCTCCCCCAGACCTTTGGAAACCCCTGGAAAACGCTGATGCC
TGGTCTCAGAACTCAACCCCATACTTGGAAGTTTATCCCCTTCTCCTGAGGTCCCGTGGT
CGGTGGCTCCACACATTCGCCAGACCGGTCCCGCCCTCTTTGTGTCTCACGTCCATGTTA
CCACCTCCCCCTATTACCCTACCCACTGCTTAAAACGCATTCCATCTGCACTCTTCCAAA
TTTACCCCCACAGTGCCAGTTTTCCTCTCC
Restriction Sites NotI-NotI     
ACCN NM_000358
Insert Size 2800 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000358.1, NP_000349.1
RefSeq Size 2691 bp
RefSeq ORF 2052 bp
Locus ID 7045
Cytogenetics 5q31.1
Domains FAS1
Protein Families Druggable Genome, Secreted Protein, Transmembrane
Gene Summary 'This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]'

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