TPMT (NM_000367) Human Untagged Clone

CAT#: SC119934

TPMT (untagged)-Human thiopurine S-methyltransferase (TPMT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TPMT
• Synonyms: TPMTD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119934 sequence for NM_000367 edited (data generated by NextGen Sequencing)
  ATGGATGGTACAAGAACTTCACTTGACATTGAAGAGTACTCGGATACTGAGGTACAGAAA
  AACCAAGTACTAACTCTGGAAGAATGGCAAGACAAGTGGGTGAACGGCAAGACTGCTTTT
  CATCAGGAACAAGGACATCAGCTATTAAAGAAGCATTTAGATACTTTCCTTAAAGGCAAG
  AGTGGACTGAGGGTATTTTTTCCTCTTTGCGGAAAAGCGGTTGAGATGAAATGGTTTGCA
  GACCGGGGACACAGTGTAGTTGGTGTGGAAATCAGTGAACTTGGGATACAAGAATTTTTT
  ACAGAGCAGAATCTTTCTTACTCAGAAGAACCAATCACCGAAATTCCTGGAACCAAAGTA
  TTTAAGAGTTCTTCGGGGAACATTTCATTGTACTGTTGCAGTATTTTTGATCTTCCCAGG
  ACAAATATTGGCAAATTTGACATGATTTGGGATAGAGGAGCATTAGTTGCCATTAATCCA
  GGTGATCGCAAATGCTATGCAGATACAATGTTTTCCCTCCTGGGAAAGAAGTTTCAGTAT
  CTCCTGTGTGTTCTTTCTTATGATCCAACTAAACATCCAGGTCCACCATTTTATGTTCCA
  CATGCTGAAATTGAAAGGTTGTTTGGTAAAATATGCAATATACGTTGTCTTGAGAAGGTT
  GATGCTTTTGAAGAACGACATAAAAGTTGGGGAATTGACTGTCTTTTTGAAAAGTTATAT
  CTACTTACAGAAAAGTAA
  
  Clone variation with respect to NM_000367.2
  474 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000367 unedited
  GTTCGGGATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGTGGAAGCG
  GAGGCGTACCCGCCCCTGGGGACGTCATTGGTGGCGGAGGCAATGGCCGGCAACCAGCTG
  TAAGCGAGGCACGGAAGACATATGCTTGTGAGACAAAGGTGTCTCTGAAACTATGGATGG
  TACAAGAACTTCACTTGACATTGAAGAGTACTCGGATACTGAGGTACAGAAAAACCAAGT
  ACTAACTCTGGAAGAATGGCAAGACAAGTGGGTGAACGGCAAGACTGCTTTTCATCAGGA
  ACAAGGACATCAGCTATTAAAGAAGCATTTAGATACTTTCCTTAAAGGCAAGAGTGGACT
  GAGGGTATTTTTTCCTCTTTGCGGAAAAGCGGTTGAGATGAAATGGTTTGCAGACCGGGG
  ACACAGTGTAGTTGGTGTGGAAATCAGTGAACTTGGGATACAAGAATTTTTTACAGAGCA
  GAATCTTTCTTACTCAGAAGAACCAATCACCGAAATTCCTGGAACCAAAGTATTTAAGAG
  TTCTTCGGGGAACATTTCATTGTACTGTTGCAGTATTTTTGATCTTCCCAGGACAAATAT
  TGGCAAATTTGACATGATTTGNGATAGAGGAGCATTAGTTGCCATTAATCCAGGTGATCG
  CAAATGCTATGCAGATACAATGTTTTCCCTCCTGGGAAAGAAGTTTCAGTATCTCCTGTG
  TGTTCTTTCTTATGATCCAACTAAACATCCACGTCCACCATTTTATGTTCCACATGCTGA
  AATTGAAAGGTTGGTTGGTAAAATATGCAATATACGTTGTCCTTGAGAAAGTTGATGCCT
  CTTGAAGACGACATAAAAGCTGGGGAATTGACTGTCTTTTTTGAAAGTATATCTACTCCA
  GCAAAGTAAATGAGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000367 unedited
  GCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTCTTTCTTTTGAGACAGAGTCTTGCTC
  TGTTGCCCAGGCTGGCGTGCAGTGGCATGATCTCAGCTCACTGCAACCTCCGCCTCCTAG
  GTTCAAGTGATTCTCCTGCCTCAGCCTCCCAAGCAGCTGGGATTACAGGCACGTGCCACC
  ACGCCTGGCTAATTTTTTTGTATTTTTAGTAGAGACGGGGTTGCTTTTCTATTCCCAAGT
  CCAAAAGCCTATGAAGAGGGCCAGCGCAATGGCTCATACCTGTAATCCTAGCACTTTGGG
  TGGCCAAGACAGGAGGACTGCTTGAGGCCTCACGTCAAGTTCAACATCAGCGTGGGCAAC
  AAAGTGAGACCCTGTCTCTACATAAAAGTAAAATCAAAAGTCTCTGGAGAGAATTGTGTT
  GGTCCAGCTCGGGTTAGTTGTCCATTCAACCGGTGATACAGGCAGAGTTGGGGAGTCATG
  GCTTCTGAAGCCCACAGTATCGACTGGGAGCAGGTCTCAGAGAAGCAAGAATCACTGTGA
  GCAAGGAGTTGCTTCAAATTAATAGCCTTACAAATCTATTAGAATACATCGATGGCAGAG
  AGCTTCTAGGGTAGAAGTTTGTACATGCAGGCCAAGGGGAACTAGAGAGGCTGANCAGAC
  ATATCTCTGGNGAGAGTACCGTAGCTTTCACCAGTGTTAAGGGGGACCCCAAACCTTGCA
  TTNAACACCTTNTAGCCAGCACATGTGCGTGCATGCGTGCACACCACACGTAACTCGTTT
  TTTATAATACACACCCATCCTTATAACATGAGCTAAANCATCTAAACCATTTTAGTTGTG
  GGTAGTACAGAGTTATAGAGGACTGTCTGATATACAAATGTTGGTAAAGAAGACTGANAA
  ATCTACCTTTTCATAATAACAGCCTGAATCCAGCTCTAGTACATTATATTTTATAGACCG
  GTGATTTAAAACGATTAATTTNANAAACAACANTNGCCNACTCAAAGAAACTTAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000367
• Insert Size: 2970 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000367.2, NP_000358.1
• RefSeq Size: 3258 bp
• RefSeq ORF: 738 bp
• Locus ID: 7172
• Cytogenetics: 6p22.3
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes
• Gene Summary: 'This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014]'