Hamartin (TSC1) (NM_000368) Human Untagged Clone

CAT#: SC119935

TSC1 (untagged)-Human tuberous sclerosis 1 (TSC1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TSC1
• Synonyms: LAM; TSC
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000368 edited
  GAATTCGGCACGAGGGCGCCCTGTAGGCTGGAGGGACTGTGAGTGACCATGAAAGACACC
  AGGTTGACAGCACTGGAAACTGAAGTACCAGTTGTCGCTAGAACAGTTTGGTAGTGGCCC
  CAATGAAGAACCTTCAGAACCTGTAGCACACGTCCTGGAGCCAGCACAGCGCCTTCGAGC
  GAGAGAATGGCCCAACAAGCAAATGTCGGGGAGCTTCTTGCCATGCTGGACTCCCCCATG
  CTGGGTGTGCGGGACGACGTGACAGCTGTCTTTAAAGAGAACCTCAATTCTGACCGTGGC
  CCTATGCTTGTAAACACCTTGGTGGATTATTACCTGGAAACCAGCTCTCAGCCGGCATTG
  CACATCCTGACCACCTTGCAAGAGCCACATGACAAGCACCTCTTGGACAGGATTAACGAA
  TATGTGGGCAAAGCCGCCACTCGTTTATCCATCCTCTCGTTACTGGGTCATGTCATAAGA
  CTGCAGCCATCTTGGAAGCATAAGCTCTCTCAAGCACCTCTTTTGCCTTCTTTACTAAAA
  TGTCTCAAGATGGACACTGACGTCGTTGTCCTCACAACAGGCGTCTTGGTGTTGATAACC
  ATGCTACCAATGATTCCACAGTCTGGGAAACAGCATCTTCTTGATTTCTTTGACATTTTT
  GGCCGTCTGTCATCATGGTGCCTGAAGAAACCAGGCCACGTGGCGGAAGTCTATCTCGTC
  CATCTCCATGCCAGTGTGTACGCACTCTTTCATCGCCTTTATGGAATGTACCCTTGCAAC
  TTCGTCTCCTTTTTGCGTTCTCATTACAGTATGAAAGAAAACCTGGAGACTTTTGAAGAA
  GTGGTCAAGCCAATGATGGAGCATGTGCGAATTCATCCGGAATTAGTGACTGGATCCAAG
  GACCATGAACTGGACCCTCGAAGGTGGAAGAGATTAGAAACTCATGATGTTGTGATCGAG
  TGTGCCAAAATCTCTCTGGATCCCACAGAAGCCTCATATGAAGATGGCTATTCTGTGTCT
  CACCAAATCTCAGCCCGCTTTCCTCATCGTTCAGCCGATGTCACCACCAGCCCTTATGCT
  GACACACAGAATAGCTATGGGTGTGCTACTTCTACCCCTTACTCCACGTCTCGGCTGATG
  TTGTTAAATATGCCAGGGCAGCTACCTCAGACTCTGAGTTCCCCATCGACACGGCTGATA
  ACTGAACCACCACAAGCTACTCTTTGGAGCCCATCTATGGTTTGTGGTATGACCACTCCT
  CCAACTTCTCCTGGAAATGTCCCACCTGATCTGTCACACCCTTACAGTAAAGTCTTTGGT
  ACAACTGCAGGTGGAAAAGGAACTCCTCTGGGAACCCCAGCAACCTCTCCTCCTCCAGCC
  CCACTCTGTCATTCGGATGACTACGTGCACATTTCACTCCCCCAGGCCACAGTCACACCC
  CCCAGGAAGGAAGAGAGAATGGATTCTGCAAGACCATGTCTACACAGACAACACCATCTT
  CTGAATGACAGAGGATCAGAAGAGCCACCTGGCAGCAAAGGTTCTGTCACTCTAAGTGAT
  CTTCCAGGGTTTTTAGGTGATCTGGCCTCTGAAGAAGATAGTATTGAAAAAGATAAAGAA
  GAAGCTGCAATATCTAGAGAACTTTCTGAGATCACCACAGCAGAGGCAGAGCCTGTGGTT
  CCTCGAGGAGGCTTTGACTCTCCCTTTTACCGAGACAGTCTCCCAGGTTCTCAGCGGAAG
  ACCCACTCGGCAGCCTCCAGTTCTCAGGGCGCCAGCGTGAACCCTGAGCCTTTACACTCC
  TCCCTGGACAAGCTTGGGCCTGACACACCAAAGCAAGCCTTTACTCCCATAGACCTGCCC
  TGCGGCAGTGCTGATGAAAGCCCTGCGGGAGACAGGGAATGCCAGACTTCTTTGGAGACC
  AGTATCTTCACTCCCAGTCCTTGTAAAATTCCACCTCCGACGAGAGTGGGCTTTGGAAGC
  GGGCAGCCTCCCCCGTATGATCATCTTTTTGAGGTGGCATTGCCAAAGACAGCCCATCAT
  TTTGTCATCAGGAAGACTGAGGAGCTGTTAAAGAAAGCAAAAGGAAACACAGAGGAAGAT
  GGTGTGCCCTCTACCTCCCCAATGGAAGTGCTGGACAGACTGATACAGCAGGGAGCAGAC
  GCGCACAGCAAGGAGCTGAACAAGTTGCCTTTACCCAGCAAGTCTGTCGACTGGACCCAC
  TTTGGAGGCTCTCCTCCTTCAGATGAGATCCGCACCCTCCGAGACCAGTTGCTTTTACTG
  CACAACCAGTTACTCTATGAGCGTTTTAAGAGGCAGCAGCATGCCCTCCGGAACAGGCGG
  CTCCTCCGCAAGGTGATCAAAGCAGCAGCTCTGGAGGAACATAATGCTGCCATGAAAGAT
  CAGTTGAAGTTACAAGAGAAGGACATCCAGATGTGGAAGGTTAGTCTGCAGAAAGAACAA
  GCTAGATACAATCAGCTCCAGGAGCAGCGTGACACTATGGTAACCAAGCTCCACAGCCAG
  ATCAGACAGCTGCAGCATGACCGAGAGGAATTCTACAACCAGAGCCAGGAATTACAGACG
  AAGCTGGAGGACTGCAGGAACATGATTGCGGAGCTGCGGATAGAACTGAAGAAGGCCAAC
  AACAAGGTGTGTCACACTGAGCTGCTGCTCAGTCAGGTTTCCCAAAAGCTCTCAAACAGT
  GAGTCGGTCCAGCAGCAGATGGAGTTCTTGAACAGGCAGCTGTTGGTTCTTGGGGAGGTC
  AACGAGCTCTATTTGGAACAACTGCAGAACAAGCACTCAGATACCACAAAGGAAGTAGAA
  ATGATGAAAGCCGCCTATCGGAAAGAGCTAGAAAAAAACAGAAGCCATGTTCTCCAGCAG
  ACTCAGAGGCTTGATACCTCCCAAAAACGGATTTTGGAACTGGAATCTCACCTGGCCAAG
  AAAGACCACCTTCTTTTGGAACAGAAGAAATATCTAGAGGATGTCAAACTCCAGGCAAGA
  GGACAGCTGCAGGCCGCAGAGAGCAGGTATGAGGCTCAGAAAAGGATAACCCAGGTGTTT
  GAATTGGAGATCTTAGATTTATATGGCAGGTTGGAGAAAGATGGCCTCCTGAAAAAACTT
  GAAGAAGAAAAAGCAGAAGCAGCTGAAGCAGCAGAAGAAAGGCTTTCTTACAGGCTTGAC
  TGTTGTAATGACGGGTGCTCAGATTCCATGGTAGGGCACAATGAAGAGGCATCTGGCCAC
  AACGGTGAGACCAAGACCCCCAGGCCCAGCAGCGCCCGGGGCAGTAGTGGAAGCAGAGGT
  GGTGGAGGCAGCAGCAGCAGCAGCAGCGAGCTTTCTACCCCAGAGAAACCCCCACACCAG
  AGGGCAGGCCCATTCAGCAGTCGGTGGGAGACGACTATGGGAGAAGCGTCTGCCAGCATC
  CCCACCACTGTGGGCTCACTTCCCAGTTCAAAAAGCTTCCTGGGTATGAAGGCTCGAGAG
  TTATTTCGTAATAAGAGCGAGAGCCAGTGTGATGAGGACGGCATGACCAGTAGCCTTTCT
  GAGAGCCTAAAGACAGAACTGGGCAAAGACTTGGGTGTGGAAGCCAAGATTCCCCTGAAC
  CTAGATGGCCCTCACCCGTCTCCCCCGACCCCGGACAGTGTTGGACAGCTACATATCATG
  GACTACAATGAGACTCATCATGAACACAGCTAAGGAATGATGGTCAATCAGTGTTAACTT
  GCATATTGTTGGCACAGAACAGGAGGTGTGAATGCACGTTTCAAAGCTTTCCTGTTTCCA
  GGGTCTGAGTGCAAGTTCATGTGTGGAAATGGGACGGAGGTCCTTTGGACAGCTGACTGA
  ATGCAGAACGGTTTTTGGATCTGGCATTGAAATGCCTCTTGACCTTCCCCTCCACCCGCC
  CTAACCCCCTCTCATTTACCTCGCAGTGTGTTCTAATCCAAGGGCCAGTTGGTGTTCCTC
  AGTAGCTTTACTTTCTTCCTTCCCCCCCAAATGGTTGCGTCCTTTGAACCTGTGCAATAT
  GAGGCCAAATTTAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000368 unedited
  AAGTTTAGATTTGTATACGACTTATATAGGCGGCCGACGAATTCGCACGAGGGCGCCCTG
  TAGGCTGGAGGGACTGTGAGTGACCATAGAAAGACACCAGGTTGACAGCACTGGAAACTG
  AAGTACCAGTTGTCGCTAGAACAGTTTGGTAGTGGCCCCAATGAAGAACCTTCAGAACCT
  GTAGCACACGTCCTGGAGCCAGCACAGCGCCTTCGAGCGAGAGAATGGCCCAACAAGCAA
  ATGTCGGGGAGCTTCTTGCCATGCTGGACTCCCCCATGCTGGGTGTGCGGGACGACGTGA
  CAGCTGTCTTTAAAGAGAACCTCAATTCTGACCGTGGCCCTATGCTTGTAAACACCTTGG
  TGGATTATTACCTGGAAACCAGCTCTCAGCCGGCATTGCACATCCTGACCACCTTGCAAG
  AGCCACATGACAAGCACCTCTTGGACAGGATTAACGAATATGTGGGCAAAGCCGCCACTC
  GTTTATCCATCCTCTCGTTACTGGGTCATGTCATAAGACTGCAGCCATCTTGGAAGCATA
  AGCTCTCTCAAGCACCTCTTTTGCCTTCTTTACTAAAATGTCTCAAGATGGACACTGACG
  TCGTTGTCCTCACAACAGGCGTCTTGGTGTTGATAACCATGCTACCAATGATTCCACAGT
  CTGGGAAACAGCATCTTCTTGATTTCTTTGACATTTTTAGCCGTCTGTCATCATGGTGCC
  TGAAGAAACCAGGCCACGTGGCANAAGTCTATCTCGTCCATCTCCATGCCAGTGTGTACG
  CACTCTTTCATCGCCTTTATGGAATGTACCCTTAGCACTTCGTCTCCTTTTTGCGTTCTC
  ATTACAGTATGAAAGAAAACCTGNAGACTTTTGNAGAAGTGGGTCAGCCCATGATGGAGC
  ATGTGCGAATTNCTCCCGGATTAGTGACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000368 unedited
  ATTAGCTNTGNNACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTAAATT
  TGGCCTCATATTGCACAGGTTCAAAGGACGCAACCATTTGGGGGGGAAGGAAGAAAGTAA
  AGCTACTGAGGAACACCAACTGGCCCTTGGATTAGAACACACTGCGAGGTAAATGAGAGG
  GGGTTAGGGCGGGTGGAGGGGAAGGTCAAGAGGCATTTCAATGCCAGATCCAAAAACCGT
  TCTGCATTCAGTCAGCTGTCCAAAGGACCTCCGTCCCATTTCCACACATGAACTTGCACT
  CAGACCCTGGAAACAGGAAAGCTTTGAAACGTGCATTCACACCTCCTGTTCTGTGCCAAC
  AATATGCAAGTTAACACTGATTGACCATCATTCCTTAGCTGTGTTCATGATGAGTCTCAT
  TGTAGTCCATGATATGTAGCTGTCCAACACTGTCCGGGGTCGGGGGAGACGGGTGAGGGC
  CATCTAGGTTCAGGGGAATCTTGGCTTCCACACCCAAGTCTTTGCCCAGTTCTGTCTTTA
  GGCTCTCAGAAAGGCTACTGGTCATGCCGTCCTCATCACACTGGCTCTCGCTCTTATTAC
  GAAATAACTCTCGAGCCTTCATACCCAGGAAGCTTTTTGAACTGTGAAGTGAGCCCACAG
  TGGTGGCGATGCTGTCAGACGCTTCTCCCATAGTCCGTTCCCACCGACTGCTGAATGGGC
  CTGCCCTCTTGTGTGGGGGGTTTCTCTGGGTACCAAGCTCGCTGCTGTCGTTGCTGATGC
  CTCCACACCTCTGTTTCCCTATTGCCCCGGAGCTGTTGGACCCGGGGGTCTGGCCTTACC
  GTTGTGGCCAGATGCTTCTCATGNGCCCTACATGGAATCTCGCCCCCTGTATTCCAACCT
  CAAGCTGCTCAACCCCCTCTCCCGTGCTTCATTCGCCTTGCCCTCCCTTTCCAATTCTTC
  CG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000368
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000368.3, NP_000359.1
• RefSeq Size: 8617 bp
• RefSeq ORF: 3495 bp
• Locus ID: 7248
• Cytogenetics: 9q34.13
• Domains: Hamartin
• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway
• Gene Summary: 'This gene is a tumor suppressor gene that encodes the growth inhibitory protein hamartin. The encoded protein interacts with and stabilizes the GTPase activating protein tuberin. This hamartin-tuberin complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signalling which is a major regulator of anabolic cell growth. This protein also functions as a co-chaperone for Hsp90 that inhibits its ATPase activity. This protein functions as a facilitator of Hsp90-mediated folding of kinase and non-kinase clients, including Tsc2 and thereby preventing their ubiquitination and proteasomal degradation. Mutations in this gene have been associated with tuberous sclerosis. [provided by RefSeq, Apr 2018]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).