alpha 1 Antitrypsin (SERPINA1) (NM_000295) Human Untagged Clone

CAT#: SC119951

SERPINA1 (untagged)-Human serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SERPINA1
• Synonyms: A1A; A1AT; AAT; alpha1AT; nNIF; PI; PI1; PRO2275
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000295, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGTCTTCTGTCTCGTGGGGCATCCTCCTGCTGGCAGGCCTGTGCTGCCTGGTCCCTGTCTCCCTGG
  CTGAGGATCCCCAGGGAGATGCTGCCCAGAAGACAGATACATCCCACCATGATCAGGATCACCCAACCTT
  CAACAAGATCACCCCCAACCTGGCTGAGTTCGCCTTCAGCCTATACCGCCAGCTGGCACACCAGTCCAAC
  AGCACCAATATCTTCTTCTCCCCAGTGAGCATCGCTACAGCCTTTGCAATGCTCTCCCTGGGGACCAAGG
  CTGACACTCACGATGAAATCCTGGAGGGCCTGAATTTCAACCTCACGGAGATTCCGGAGGCTCAGATCCA
  TGAAGGCTTCCAGGAACTCCTCCGTACCCTCAACCAGCCAGACAGCCAGCTCCAGCTGACCACCGGCAAT
  GGCCTGTTCCTCAGCGAGGGCCTGAAGCTAGTGGATAAGTTTTTGGAGGATGTTAAAAAGTTGTACCACT
  CAGAAGCCTTCACTGTCAACTTCGGGGACACCGAAGAGGCCAAGAAACAGATCAACGATTACGTGGAGAA
  GGGTACTCAAGGGAAAATTGTGGATTTGGTCAAGGAGCTTGACAGAGACACAGTTTTTGCTCTGGTGAAT
  TACATCTTCTTTAAAGGCAAATGGGAGAGACCCTTTGAAGTCAAGGACACCGAGGAAGAGGACTTCCACG
  TGGACCAGGTGACCACCGTGAAGGTGCCTATGATGAAGCGTTTAGGCATGTTTAACATCCAGCACTGTAA
  GAAGCTGTCCAGCTGGGTGCTGCTGATGAAATACCTGGGCAATGCCACCGCCATCTTCTTCCTGCCTGAT
  GAGGGGAAACTACAGCACCTGGAAAATGAACTCACCCACGATATCATCACCAAGTTCCTGGAAAATGAAG
  ACAGAAGGTCTGCCAGCTTACATTTACCCAAACTGTCCATTACTGGAACCTATGATCTGAAGAGCGTCCT
  GGGTCAACTGGGCATCACTAAGGTCTTCAGCAATGGGGCTGACCTCTCCGGGGTCACAGAGGAGGCACCC
  CTGAAGCTCTCCAAGGCCGTGCATAAGGCTGTGCTGACCATCGACGAGAAAGGGACTGAAGCTGCTGGGG
  CCATGTTTTTAGAGGCCATACCCATGTCTATCCCCCCCGAGGTCAAGTTCAACAAACCCTTTGTCTTCTT
  AATGATTGAACAAAATACCAAGTCTCCCCTCTTCATGGGAAAAGTGGTGAATCCCACCCAAAAATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000295 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCAGGTTGGAGGGG
  CGGCAACCTCCTGCCAGCCTTCAGGCCACTCTCCTGTGCCTGCCAGAAGAGACAGAGCTT
  GAGGAGAGCTTGAGGAGAGCAGGAAAGGACAATGCCGTCTTCTGTCTCGTGGGGCATCCT
  CCTGCTGGCAGGCCTGTGCTGCCTGGTCCCTGTCTCCCTGGCTGAGGATCCCCAGGGAGA
  TGCTGCCCAGAAGACAGATACATCCCACCATGATCAGGATCACCCAACCTTCAACAAGAT
  CACCCCCAACCTGGCTGAGTTCGCCTTCAGCCTATACCGCCAGCTGGCACACCAGTCCAA
  CAGCACCAATATCTTCTTCTCCCCAGTGAGCATCGCTACAGCCTTTGCAATGCTCTCCCT
  GGGGACCAAGGCTGACACTCACGATGAAATCCTGGAGGGCCTGAATTTCAACCTCACGGA
  GATTCCGGAGGCTCAGATCCATGAAGGCTTCCAGGAACTCCTCCGTACCCTCAACCAGCC
  AGACAGCCAGCTCCAGCTGACCACCGGCAATGGCCTGTTCCTCAGCGAGGGCCTGAAGCT
  AGTGGATAAGTTTTTGGAGGATGTTAAAAAGTTGTACCACTCAGAAGCCTTCACTGTCAA
  CTTCGGGGACACCGAAGAGGCCAAGAAACAGATCAACGATTACGTGGAGAANGGTACTCA
  AGGGAAAATTGTGGATTTGGTCAAGGAGCTTGACAGAGACACAGTTTTTGCTCTGGTGAA
  TTACATCTTCTTTAAAGGCAATGGGAGAGACCCTTTGAAGTCAAGACACCGAGAAGAGGA
  CTTCCGTGNACCAGTGACACCGTGAAGTGCTATGAGAGCGTTTAGCATGTTAACTCCACC
  TGTAGAGCTGTCACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000295 unedited
  CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCAGGCAGGGACCAGCTCAACCCT
  TCTTTAATGTCATCCAGGGAGGGGGCCAGGGATGGAGGGGAGGGGTTGAGGAGCGAGAGG
  CAGTTATTTTTGGGTGGGATTCACCACTTTTCCCATGAAGAGGGGAGACTTGGTATTTTG
  TTCAATCATTAAGAAGACAAAGGGTTTGTTGAACTTGACCTCGGGGGGGATAGACATGGG
  TATGGCCTCTAAAAACATGGCCCCAGCAGCTTCAGTCCCTTTCTCGTCGATGGTCAGCAC
  AGCCTTATGCACGGCCTTGGAGAGCTTCAGGGGTGCCTCCTCTGTGACCCCGGAGAGGTC
  AGCCCCATTGCTGAAGACCTTAGTGATGCCCAGTTGACCCAGGACGCTCTTCAGATCATA
  GGTTCCAGTAATGGACAGTTTGGGTAAATGTAAGCTGGCAGACCTTCTGTCTTCATTTTC
  CAGGAACTTGGTGATGATATCGCGGGTGAGTTCATTTTCCAGGTGCTGTAGTTTCCCCTC
  CTCATGCAAGAAGAATATGGCCGTGGCATTTGCCCAGGTATTTCATCAGCAGCACCCAGC
  TGGACAGCTTCTACAGGGCTGGATGTAAACATGCCTAAACGCTTCATCATAAGCACCTTC
  ACGGGGGCCACCTGGCCCCGTGGAAGTCCTCTTTCTCGGTGCCCCTGACTCCAAAGGGCT
  CTCCCTTTGCCTTTAAAGAGATGTATTTCCCACACCAAAACTGCGTTTTTGTAACCCCCT
  GGACCAATCCCAATTTTCCTTGAGACCCCTCTCCACTAATCGTGGAACCGCCTCTCGCCC
  CCTTCGGGGCCCCCAATTTACATTAAGGCTTCTCATGTCCCCTTTTTATATCCCCAAAAA
  CTTATCCTCTTTCTGCCCTTCTTGAATAAAGCCCTTCCCGCGGCCCTCCCCACTCCTCCC
  GCCTGCCGGAGGCCGAATAATCCTTCAACN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000295
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000295.3, NP_000286.3
• RefSeq Size: 1607 bp
• RefSeq ORF: 1257 bp
• Locus ID: 5265
• Cytogenetics: 14q32.13
• Domains: SERPIN
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: 'The protein encoded by this gene is a serine protease inhibitor belonging to the serpin superfamily whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. This protein is produced in the liver, the bone marrow, by lymphocytic and monocytic cells in lymphoid tissue, and by the Paneth cells of the gut. Defects in this gene are associated with chronic obstructive pulmonary disease, emphysema, and chronic liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2020]'
  Transcript Variant: This variant (1) encodes the same protein as the other ten variants of this gene. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.