PLOD1 (NM_000302) Human Untagged Clone

CAT#: SC119956

PLOD1 (untagged)-Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1)


  "NM_000302" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "PLOD1"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PLOD1
Synonyms EDS6; EDSKCL1; LH; LH1; LLH; PLOD
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_000302 edited
ATGCGGCCCCTGCTGCTACTGGCCCTGCTGGGCTGGCTGCTGCTGGCCGAAGCGAAGGGC
GACGCCAAGCCGGAGGACAACCTTTTAGTCCTCACGGTGGCCACTAAGGAGACCGAGGGA
TTCCGTCGCTTCAAGCGCTCAGCTCAGTTCTTCAACTACAAGATCCAGGCGCTTGGCCTA
GGGGAGGACTGGAATGTGGAGAAGGGGACGTCGGCAGGTGGAGGGCAGAAGGTCCGGCTG
CTGAAGAAAGCTCTGGAGAAGCACGCAGACAAGGAGGATCTGGTCATTCTCTTCGCAGAC
AGCTATGACGTGCTGTTTGCATCGGGGCCCCGGGAGCTCCTGAAGAAGTTCCGGCAGGCC
AGGAGCCAGGTGGTCTTCTCTGCTGAGGAGCTCATCTACCCAGACCGCAGGCTGGAGACC
AAGTATCCGGTGGTGTCCGATGGCAAGAGGTTCCTGGGCTCTGGAGGCTTCATCGGTTAT
GCCCCCAACCTCAGCAAACTGGTGGCCGAGTGGGAGGGCCAGGACAGCGACAGCGATCAG
CTGTTTTACACCAAGATCTTCTTGGACCCGGAGAAGAGGGAGCAGATCAATATCACCCTG
GACCACCGCTGCCGTATCTTCCAGAACCTGGATGGAGCCTTGGATGAGGTCGTGCTCAAG
TTTGAAATGGGCCATGTGAGAGCGAGGAACCTGGCCTATGACACCCTCCCGGTCCTGATC
CATGGCAACGGGCCAACCAAGCTGCAGTTGAACTACCTGGGCAACTACATCCCGCGCTTC
TGGACCTTCGAAACAGGCTGCACCGTGTGTGACGAAGGCTTGCGCAGCCTCAAGGGCATT
GGGGATGAAGCTCTGCCCACGGTCCTGGTCGGCGTGTTCATCGAACAGCCCACGCCGTTT
GTGTCCCTGTTCTTCCAGCGGCTCCTGCGGCTCCACTACCCCCAGAAACACATGCGACTT
TTCATCCACAACCACGAGCAGCACCACAAGGCTCAGGTGGAAGAGTTCCTGGCACAGCAT
GGCAGCGAGTACCAGTCTGTGAAGCTGGTGGGCCCTGAGGTGCGGATGGCGAATGCAGAT
GCCAGGAACATGGGCGCAGACCTGTGCCGGCAGGACCGCAGCTGCACCTACTACTTCAGC
GTGGATGCTGACGTGGCCCTGACCGAGCCCAACAGCCTGCGGCTGCTGATCCAACAGAAC
AAGAACGTCATTGCCCCGCTGATGACCCGGCATGGGAGGCTGTGGTCGAACTTCTGGGGG
GCTCTCAGTGCAGATGGCTACTATGCCCGTTCCGAGGACTACGTGGACATTGTGCAGGGG
CGGCGTGTTGGTGTCTGGAATGTGCCCTATATTTCAAACATCTACTTGATCAAGGGCAGT
GCCCTGCGGGGTGAGCTGCAGTCCTCAGATCTCTTCCACCACAGCAAGCTGGACCCCGAC
ATGGCCTTCTGTGCCAACATCCGGCAGCAGGATGTGTTCATGTTCCTGACCAACCGGCAC
ACCCTTGGCCATCTGCTCTCCCTAGACAGCTACCGCACCACCCACCTGCACAACGACCTC
TGGGAGGTGTTCAGCAACCCCGAGGACTGGAAGGAGAAGTACATCCACCAGAACTACACC
AAAGCCCTGGCAGGGAAGCTGGTGGAGACGCCCTGCCCGGATGTCTATTGGTTCCCCATC
TTCACGGAGGTGGCCTGTGATGAGCTGGTGGAGGAGATGGAGCACTTTGGCCAGTGGTCT
CTGGGCAACAACAAGGACAACCGCATCCAGGGTGGCTACGAGAACGTGCCGACTATTGAC
ATCCACATGAACCAGATCGGCTTTGAGCGGGAGTGGCACAAATTCCTGCTGGAGTACATT
GCGCCCATGACGGAGAAGCTCTACCCCGGCTACTACACCAGGGCCCAGTTTGACCTGGCC
TTTGTCGTCCGCTACAAGCCTGATGAGCAGCCCTCACTGATGCCACACCATGATGCCTCC
ACCTTCACCATCAACATCGCCCTGAACCGAGTCGGGGTGGATTACGAGGGCGGGGGCTGT
CGGTTCCTGCGCTACAACTGTTCCATCCGAGCCCCAAGGAAGGGCTGGACCCTCATGCAC
CCTGGACGACTCACGCATTACCATGAGGGGCTCCCCACCACCAGGGGCACCCGCTACATC
GCAGTCTCCTTCGTCGATCCCTAA
>OriGene 5' read for NM_000302 unedited
NGGTGTTCAAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGGAC
CTGCGGCCCCGNTCGCGAGTTTCCAGCCCTGCGAGCGCCGCCGGGTCGGCCGATCGTCCC
CCATACCTCGGCCATGCGGCCCCTGCTGCTACTGGCCCTGCTGGGCTGGCTGCTGCTGGC
CGAAGCGAAGGGCGACGCCAAGCCGGAGGACAACCTTTTAGTCCTCACGGTGGCCACTAA
GGAGACCGAGGGATTCCGTCGCTTCAAGCGCTCAGCTCAGTTCTTCAACTACAAGATCCA
GGCGCTTGGCCTAGGGGAGGACTGGAATGTGGAGAAGGGGACGTCGGCAGGTGGAGGGCA
GAAGGTCCGGCTGCTGAAGAAAGCTCTGGAGAAGCACGCAGACAAGGAGGATCTGGTCAT
TCTCTTCGCAGACAGCTATGACGTGCTGTTTGCATCGGGGCCCCGGGAGCTCCTGAAGAA
GTTCCGGCAGGCCAGGAGCCAGGTGGTCTTCTCTGCTGAGGAGCTCATCTACCCAGACCG
CAGGCTGGAGACCAAGTATCCGGTGGTGTCCGATGGCAAGAGGTTCCTGGGCTCTGGAGG
CTTCATCGGTTATGCCCCCAACCTCAGCAAACTGGTGGCCGAGTGGGAGGGCCAGGACAG
CGACAGCGATCAGCTGTTTTACACCAAGATCTTCTTGGACCCGGAGAAGAAGGAGCAGAT
CAATATCACCCTGGACCACCGCTGCCGTATCTTCCAGACCTGGATGGAGCCTTGGATGAA
GTCGTGCTCAAGTTTGAAATGGCCATGTGAGAGCGAAGAACCTGGCCTATGACACCCTCC
CCGTCCTGATCCATGGCAAACGGGCCACCAAGCTGCAGTTGAACTACCTGGNGCACTACA
TNCCG
>OriGene 3' read for NM_000302 unedited
CTTTATAGAACTCTCCGTTTCTGACGGTCTGTCCCTCTCTTCANAGCATCCAGGACAGGG
TGACCCAGTGTGGTGTGTGGACCTGTCACTCCCCTCCCCACAGGCAAGTAGAAGATCGAG
CTGTGCACAGATGCCTCTTCTGTCTCCCAAGAGGTTCTCTGGAGTCGCTGTCTCCAGGGG
ACTACGGTTTGCTCTCATGACCAGCCATGGAGGCAGAAGTCCCAGACTCAGGGCAGCCCT
TGGAGTCTGATCCAGGTGTCTTTACCCCAAAACTTGAAGCAGAAGTCCCTGGTGCAACAC
CTGTGCCTGGGAGAAGCTCTGGCCATGGCTTCTCACCCAGACAGTCTTTCTGGGCAACTT
GGGGAAGCCCCTGTTCTGCTCAAGTCTCACCCCATGGAAGAGGTGGGGGAAGGGGGCCTT
GGTTTTTCAGGAAGACAGGTTGGAGAGCACGAGTCACTACAAAGCAGTAAAAGTGAATGG
TGTCTCCAGGGGCTGGGTCCAGAACACCACGGAGAGCCCCAGCCATAAAGGTGTGTTCCG
CCTCTGGCCTGCAGGAATCTCTTTGAATCTCTNTGATTGGTGGCTCCAAGAGCAATGGGA
AGTCAACAGCCCAGAGGCTGGACTGNGTTCCCTGGGGACCCCGAGTCCCANNAGCTGCTG
GGCAGTGGTTGTCGGCAAAGAAAGAAGGTCCCAGAGGGTCANGCCTGCCCAATANGGATC
GACGAAGGAGACTGCGATGTACCGGGTGCCCCTGTTGGTGGGGAGCCCCTCATGGTATGC
GTGAGTCGTCCAGGGTGCATGAGGGTTCACCCTTNCCTTGGGGCTCGAATGGACCAGTTG
TACGCANGACCCGACAGNCCCCGCCTCGTNATCCACCCGACTGCGTCAGGGCCATTGTGA
TGGTGAAGGCTGAGGCCTCATGGTGTGGCACTCATGAAGCCTGCTATCAAGTCTGATACG
AACACAAGGCAGGTCAACTGGGCCCTGTGTATTACCGGGTAAAGCTTCCCTCATGGCCAT
Restriction Sites NotI-NotI     
ACCN NM_000302
Insert Size 3180 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000302.2, NP_000293.2
RefSeq Size 3004 bp
RefSeq ORF 2184 bp
Locus ID 5351
Cytogenetics 1p36.22
Domains 2OG-FeII_Oxy, P4Hc
Protein Families Druggable Genome
Protein Pathways Lysine degradation
Gene Summary 'Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]'
Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.