PLOD1 (NM_000302) Human Untagged Clone

CAT#: SC119956

PLOD1 (untagged)-Human procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1 (PLOD1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLOD1
• Synonyms: EDS6; EDSKCL1; LH; LH1; LLH; PLOD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000302 edited
  ATGCGGCCCCTGCTGCTACTGGCCCTGCTGGGCTGGCTGCTGCTGGCCGAAGCGAAGGGC
  GACGCCAAGCCGGAGGACAACCTTTTAGTCCTCACGGTGGCCACTAAGGAGACCGAGGGA
  TTCCGTCGCTTCAAGCGCTCAGCTCAGTTCTTCAACTACAAGATCCAGGCGCTTGGCCTA
  GGGGAGGACTGGAATGTGGAGAAGGGGACGTCGGCAGGTGGAGGGCAGAAGGTCCGGCTG
  CTGAAGAAAGCTCTGGAGAAGCACGCAGACAAGGAGGATCTGGTCATTCTCTTCGCAGAC
  AGCTATGACGTGCTGTTTGCATCGGGGCCCCGGGAGCTCCTGAAGAAGTTCCGGCAGGCC
  AGGAGCCAGGTGGTCTTCTCTGCTGAGGAGCTCATCTACCCAGACCGCAGGCTGGAGACC
  AAGTATCCGGTGGTGTCCGATGGCAAGAGGTTCCTGGGCTCTGGAGGCTTCATCGGTTAT
  GCCCCCAACCTCAGCAAACTGGTGGCCGAGTGGGAGGGCCAGGACAGCGACAGCGATCAG
  CTGTTTTACACCAAGATCTTCTTGGACCCGGAGAAGAGGGAGCAGATCAATATCACCCTG
  GACCACCGCTGCCGTATCTTCCAGAACCTGGATGGAGCCTTGGATGAGGTCGTGCTCAAG
  TTTGAAATGGGCCATGTGAGAGCGAGGAACCTGGCCTATGACACCCTCCCGGTCCTGATC
  CATGGCAACGGGCCAACCAAGCTGCAGTTGAACTACCTGGGCAACTACATCCCGCGCTTC
  TGGACCTTCGAAACAGGCTGCACCGTGTGTGACGAAGGCTTGCGCAGCCTCAAGGGCATT
  GGGGATGAAGCTCTGCCCACGGTCCTGGTCGGCGTGTTCATCGAACAGCCCACGCCGTTT
  GTGTCCCTGTTCTTCCAGCGGCTCCTGCGGCTCCACTACCCCCAGAAACACATGCGACTT
  TTCATCCACAACCACGAGCAGCACCACAAGGCTCAGGTGGAAGAGTTCCTGGCACAGCAT
  GGCAGCGAGTACCAGTCTGTGAAGCTGGTGGGCCCTGAGGTGCGGATGGCGAATGCAGAT
  GCCAGGAACATGGGCGCAGACCTGTGCCGGCAGGACCGCAGCTGCACCTACTACTTCAGC
  GTGGATGCTGACGTGGCCCTGACCGAGCCCAACAGCCTGCGGCTGCTGATCCAACAGAAC
  AAGAACGTCATTGCCCCGCTGATGACCCGGCATGGGAGGCTGTGGTCGAACTTCTGGGGG
  GCTCTCAGTGCAGATGGCTACTATGCCCGTTCCGAGGACTACGTGGACATTGTGCAGGGG
  CGGCGTGTTGGTGTCTGGAATGTGCCCTATATTTCAAACATCTACTTGATCAAGGGCAGT
  GCCCTGCGGGGTGAGCTGCAGTCCTCAGATCTCTTCCACCACAGCAAGCTGGACCCCGAC
  ATGGCCTTCTGTGCCAACATCCGGCAGCAGGATGTGTTCATGTTCCTGACCAACCGGCAC
  ACCCTTGGCCATCTGCTCTCCCTAGACAGCTACCGCACCACCCACCTGCACAACGACCTC
  TGGGAGGTGTTCAGCAACCCCGAGGACTGGAAGGAGAAGTACATCCACCAGAACTACACC
  AAAGCCCTGGCAGGGAAGCTGGTGGAGACGCCCTGCCCGGATGTCTATTGGTTCCCCATC
  TTCACGGAGGTGGCCTGTGATGAGCTGGTGGAGGAGATGGAGCACTTTGGCCAGTGGTCT
  CTGGGCAACAACAAGGACAACCGCATCCAGGGTGGCTACGAGAACGTGCCGACTATTGAC
  ATCCACATGAACCAGATCGGCTTTGAGCGGGAGTGGCACAAATTCCTGCTGGAGTACATT
  GCGCCCATGACGGAGAAGCTCTACCCCGGCTACTACACCAGGGCCCAGTTTGACCTGGCC
  TTTGTCGTCCGCTACAAGCCTGATGAGCAGCCCTCACTGATGCCACACCATGATGCCTCC
  ACCTTCACCATCAACATCGCCCTGAACCGAGTCGGGGTGGATTACGAGGGCGGGGGCTGT
  CGGTTCCTGCGCTACAACTGTTCCATCCGAGCCCCAAGGAAGGGCTGGACCCTCATGCAC
  CCTGGACGACTCACGCATTACCATGAGGGGCTCCCCACCACCAGGGGCACCCGCTACATC
  GCAGTCTCCTTCGTCGATCCCTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000302 unedited
  NGGTGTTCAAAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGGAC
  CTGCGGCCCCGNTCGCGAGTTTCCAGCCCTGCGAGCGCCGCCGGGTCGGCCGATCGTCCC
  CCATACCTCGGCCATGCGGCCCCTGCTGCTACTGGCCCTGCTGGGCTGGCTGCTGCTGGC
  CGAAGCGAAGGGCGACGCCAAGCCGGAGGACAACCTTTTAGTCCTCACGGTGGCCACTAA
  GGAGACCGAGGGATTCCGTCGCTTCAAGCGCTCAGCTCAGTTCTTCAACTACAAGATCCA
  GGCGCTTGGCCTAGGGGAGGACTGGAATGTGGAGAAGGGGACGTCGGCAGGTGGAGGGCA
  GAAGGTCCGGCTGCTGAAGAAAGCTCTGGAGAAGCACGCAGACAAGGAGGATCTGGTCAT
  TCTCTTCGCAGACAGCTATGACGTGCTGTTTGCATCGGGGCCCCGGGAGCTCCTGAAGAA
  GTTCCGGCAGGCCAGGAGCCAGGTGGTCTTCTCTGCTGAGGAGCTCATCTACCCAGACCG
  CAGGCTGGAGACCAAGTATCCGGTGGTGTCCGATGGCAAGAGGTTCCTGGGCTCTGGAGG
  CTTCATCGGTTATGCCCCCAACCTCAGCAAACTGGTGGCCGAGTGGGAGGGCCAGGACAG
  CGACAGCGATCAGCTGTTTTACACCAAGATCTTCTTGGACCCGGAGAAGAAGGAGCAGAT
  CAATATCACCCTGGACCACCGCTGCCGTATCTTCCAGACCTGGATGGAGCCTTGGATGAA
  GTCGTGCTCAAGTTTGAAATGGCCATGTGAGAGCGAAGAACCTGGCCTATGACACCCTCC
  CCGTCCTGATCCATGGCAAACGGGCCACCAAGCTGCAGTTGAACTACCTGGNGCACTACA
  TNCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000302 unedited
  CTTTATAGAACTCTCCGTTTCTGACGGTCTGTCCCTCTCTTCANAGCATCCAGGACAGGG
  TGACCCAGTGTGGTGTGTGGACCTGTCACTCCCCTCCCCACAGGCAAGTAGAAGATCGAG
  CTGTGCACAGATGCCTCTTCTGTCTCCCAAGAGGTTCTCTGGAGTCGCTGTCTCCAGGGG
  ACTACGGTTTGCTCTCATGACCAGCCATGGAGGCAGAAGTCCCAGACTCAGGGCAGCCCT
  TGGAGTCTGATCCAGGTGTCTTTACCCCAAAACTTGAAGCAGAAGTCCCTGGTGCAACAC
  CTGTGCCTGGGAGAAGCTCTGGCCATGGCTTCTCACCCAGACAGTCTTTCTGGGCAACTT
  GGGGAAGCCCCTGTTCTGCTCAAGTCTCACCCCATGGAAGAGGTGGGGGAAGGGGGCCTT
  GGTTTTTCAGGAAGACAGGTTGGAGAGCACGAGTCACTACAAAGCAGTAAAAGTGAATGG
  TGTCTCCAGGGGCTGGGTCCAGAACACCACGGAGAGCCCCAGCCATAAAGGTGTGTTCCG
  CCTCTGGCCTGCAGGAATCTCTTTGAATCTCTNTGATTGGTGGCTCCAAGAGCAATGGGA
  AGTCAACAGCCCAGAGGCTGGACTGNGTTCCCTGGGGACCCCGAGTCCCANNAGCTGCTG
  GGCAGTGGTTGTCGGCAAAGAAAGAAGGTCCCAGAGGGTCANGCCTGCCCAATANGGATC
  GACGAAGGAGACTGCGATGTACCGGGTGCCCCTGTTGGTGGGGAGCCCCTCATGGTATGC
  GTGAGTCGTCCAGGGTGCATGAGGGTTCACCCTTNCCTTGGGGCTCGAATGGACCAGTTG
  TACGCANGACCCGACAGNCCCCGCCTCGTNATCCACCCGACTGCGTCAGGGCCATTGTGA
  TGGTGAAGGCTGAGGCCTCATGGTGTGGCACTCATGAAGCCTGCTATCAAGTCTGATACG
  AACACAAGGCAGGTCAACTGGGCCCTGTGTATTACCGGGTAAAGCTTCCCTCATGGCCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000302
• Insert Size: 3180 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000302.2, NP_000293.2
• RefSeq Size: 3004 bp
• RefSeq ORF: 2184 bp
• Locus ID: 5351
• Cytogenetics: 1p36.22
• Domains: 2OG-FeII_Oxy, P4Hc
• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation
• Gene Summary: 'Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.