PPT1 (NM_000310) Human Untagged Clone

CAT#: SC119961

PPT1 (untagged)-Human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPT1
• Synonyms: CLN1; INCL; PPT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119961 sequence for NM_000310 edited (data generated by NextGen Sequencing)
  ATGGCGTCGCCCGGCTGCCTGTGGCTCTTGGCTGTGGCTCTCCTGCCATGGACCTGCGCT
  TCTCGGGCGCTGCAGCATCTGGACCCGCCGGCGCCGCTGCCGTTGGTGATCTGGCATGGG
  ATGGGAGACAGCTGTTGCAATCCCTTAAGCATGGGTGCTATTAAAAAAATGGTGGAGAAG
  AAAATACCTGGAATTTACGTCTTATCTTTAGAGATTGGGAAGACCCTGATGGAGGACGTG
  GAGAACAGCTTCTTCTTGAATGTCAATTCCCAAGTAACAACAGTGTGTCAGGCACTTGCT
  AAGGATCCTAAATTGCAGCAAGGCTACAATGCTATGGGATTCTCCCAGGGAGGCCAATTT
  CTGAGGGCAGTGGCTCAGAGATGCCCTTCACCTCCCATGATCAATCTGATCTCGGTTGGG
  GGACAACATCAAGGTGTTTTTGGACTCCCTCGATGCCCAGGAGAGAGCTCTCACATCTGT
  GACTTCATCCGAAAAACACTGAATGCTGGGGCGTACTCCAAAGTTGTTCAGGAACGCCTC
  GTGCAAGCCGAATACTGGCATGACCCCATAAAGGAGGATGTGTATCGCAACCACAGCATC
  TTCTTGGCAGATATAAATCAGGAGCGGGGTATCAATGAGTCCTACAAGAAAAACCTGATG
  GCCCTGAAGAAGTTTGTGATGGTGAAATTCCTCAATGATTCCATTGTGGACCCTGTAGAT
  TCGGAGTGGTTTGGATTTTACAGAAGTGGCCAAGCCAAGGAAACCATTCCCTTACAGGAG
  ACCTCCCTGTACACACAGGACCGCCTGGGGCTAAAGGAAATGGACAATGCAGGACAGCTA
  GTGTTTCTGGCTACAGAAGGGGACCATCTTCAGTTGTCTGAAGAATGGTTTTATGCCCAC
  ATCATACCATTCCTTGGATGA
  
  Clone variation with respect to NM_000310.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000310 unedited
  GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACACAGCGAAGATGGCG
  TCGCCCGGCTGCCTGTGGCTCTTGGCTGTGGCTCTCCTGCCATGGACCTGCGCTTCTCGG
  GCGCTGCAGCATCTGGACCCGCCGGCGCCGCTGCCGTTGGTGATCTGGCATGGGATGGGA
  GACAGCTGTTGCAATCCCTTAAGCATGGGTGCTATTAAAAAAATGGTGGAGAAGAAAATA
  CCTGGAATTTACGTCTTATCTTTAGAGATTGGGAAGACCCTGATGGAGGACGTGGAGAAC
  AGCTTCTTCTTGAATGTCAATTCCCAAGTAACAACAGTGTGTCAGGCACTTGCTAAGGAT
  CCTAAATTGCAGCAAGGCTACAATGCTATGGGATTCTCCCAGGGAGGCCAATTTCTGAGG
  GCAGTGGCTCAGAGATGCCCTTCACCTCCCATGATCAATCTGATCTCGGTTGGGGGACAA
  CATCAAGGTGTTTTTGGACTCCCTCGATGCCCAGGAGAGAGCTCTCACATCTGTGACTTC
  ATCCGAAAAACACTGAATGCTGGGGCGTACTCCAAAGTTGTTCAGGAACGCCTCGTGCAA
  GCCGAATACTGGCATGACCCCATAAAGGAGGATGTGTATCGCAACCACAGCATCTTCTTG
  GCAGATATAAATCAGGAGCGGNGTATCAATGAGTCCTACAGNAAAACCTGATGGCCCTGA
  AGAAGTTTGTGATGGTGAAATTCCTCAATGATTCCATTGTGGNACCCTGTAGATTCNGNA
  GTGGTTGGNNATTTACAGAAAGTGGCCAAGCCAAGGAAACCATTCCCTTACAGAGAACCT
  CCTGTCACACAGNACCGNNCTGGGCTAAAGGAATGGACATGCAGACAGCTAGTGTTTCTG
  CTCAGAGGNGACCATCTCAGTGCTGAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000310 unedited
  ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAAAACTACTCTTTA
  TTTTAGACAACCTCATAAAATTATTTTCACATCCCCCCCAACTTCTTGCTCTTAATCCTC
  ATCTTTTAGTTGAAGAATAAGGCTTAAGAGAGAGAAAGGAAAAACCATAATGGCTAAACT
  TAGCAGCACCAACACGGTTCTTTCATCAAGGCGTAGCTCATCATTTCTCCAAACTGACAT
  GCTACAGAAATGTCTTCCAAAAAGCGTTAAGTTTTCACAGAGTGGGGACTATGATTTCCA
  TGCTCAAATAGTGTAGGAAATGGTAGATTACGTTAGTTTTGTTTACTGTAACAGGAATTC
  TCAGGAGTCCTTAGACACTCTAATGTGGGTTGATCTCCAGAAGGAGAAAGGGAGTGTGTA
  GTATTTTCCAAGCTTTTTGAGCAAACAATCCTTTTCTCATGGAACACATCAGAAACAGTT
  TGGAGTATGCTGGTAAATGTGTTTTGGGGATGGAAGTCAGAAAGCCTGATCTTTTTCATA
  TGGTTCCTCCTATTCTCTGCTAAAGCCAGCAACAGAGTAAGTTGACTCTAGCATCAAGAC
  CACTAGTCATATTAAGAAAGGGCAGGTTGGTCGAGAATGGAATAGGAAGAACTAGCTGCC
  TCACTTTTCACTTTCGCGCCTACATTTTCTTCGCTCCCTAAGTTATACAAGATGTACATT
  TCCTCTCTTAACAATTTATTTGGGTGCTACTTCCCCTCCTTTGTGACGCAACTATTTATC
  ACTACCTGAGGAGACAAAGTGGGTGCTGCATAAGCAAGACTTGTAGCCAGGGGGATTGCC
  TATAATTGAGATAAGAGATTGATGCCCCNAGGGCACGTCTCAAAAGGAAGCGCGCTTAAA
  AAGATGCGAATACCAGCAGATTTTCAGCACTGTTAATACACCAACAAGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000310
• Insert Size: 2610 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000310.2, NP_000301.1
• RefSeq Size: 2358 bp
• RefSeq ORF: 921 bp
• Locus ID: 5538
• Cytogenetics: 1p34.2
• Domains: Palm_thioest
• Protein Families: Druggable Genome
• Protein Pathways: Fatty acid elongation in mitochondria, Lysosome, Metabolic pathways
• Gene Summary: 'The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.