PDHA1 (NM_000284) Human Untagged Clone

CAT#: SC119981

PDHA1 (untagged)-Human pyruvate dehydrogenase (lipoamide) alpha 1 (PDHA1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDHA1
• Synonyms: PDHA; PDHAD; PDHCE1A; PHE1A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119981 sequence for NM_000284 edited (data generated by NextGen Sequencing)
  ATGAGGAAGATGCTCGCCGCCGTCTCCCGCGTGCTGTCTGGCGCTTCTCAGAAGCCGGCA
  AGCAGAGTGCTGGTAGCATCCCGTAATTTTGCAAATGATGCTACATTTGAAATTAAGAAA
  TGTGACCTTCACCGGCTGGAAGAAGGCCCTCCTGTCACAACAGTGCTCACCAGGGAGGAT
  GGGCTCAAATACTACAGGATGATGCAGACTGTACGCCGAATGGAGTTGAAAGCAGATCAG
  CTGTATAAACAGAAAATTATTCGTGGTTTCTGTCACTTGTGTGATGGTCAGGAAGCTTGC
  TGTGTGGGCCTGGAGGCCGGCATCAACCCCACAGACCATCTCATCACAGCCTACCGGGCT
  CACGGCTTTACTTTCACCCGGGGCCTTTCCGTCCGAGAAATTCTCGCAGAGCTTACAGGA
  CGAAAAGGAGGTTGTGCTAAAGGGAAAGGAGGATCGATGCACATGTATGCCAAGAACTTC
  TACGGGGGCAATGGCATCGTGGGAGCGCAGGTGCCCCTGGGCGCTGGGATTGCTCTAGCC
  TGTAAGTATAATGGAAAAGATGAGGTCTGCCTGACTTTATATGGCGATGGTGCTGCTAAC
  CAGGGCCAGATATTCGAAGCTTACAACATGGCAGCTTTGTGGAAATTACCTTGTATTTTC
  ATCTGTGAGAATAATCGCTATGGAATGGGAACGTCTGTTGAGAGAGCGGCAGCCAGCACT
  GATTACTACAAGAGAGGCGATTTCATTCCTGGGCTGAGAGTGGATGGAATGGATATCCTG
  TGCGTCCGAGAGGCAACAAGGTTTGCTGCTGCCTATTGTAGATCTGGGAAGGGGCCCATC
  CTGATGGAGCTGCAGACTTACCGTTACCACGGACACAGTATGAGTGACCCTGGAGTCAGT
  TACCGTACACGAGAAGAAATTCAGGAAGTAAGAAGTAAGAGTGACCCTATTATGCTTCTC
  AAGGACAGGATGGTGAACAGCAATCTTGCCAGTGTGGAAGAACTAAAGGAAATTGATGTG
  GAAGTGAGGAAGGAGATTGAGGATGCTGCCCAGTTTGCCACGGCCGATCCTGAGCCACCT
  TTGGAAGAGCTGGGCTACCACATCTACTCCAGCGACCCACCTTTTGAAGTTCGTGGTGCC
  AATCAGTGGATCAAGTTTAAGTCAGTCAGTTAA
  
  Clone variation with respect to NM_000284.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000284 unedited
  GGGCACCATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGTCTGCTGG
  GGCACCTGAAGGAGACTTGGGGGCACCCGCGTCGTGCCTCCTGGGTTGTGAGGAGTCGCC
  GCTGCCGCCACTGCCTGTGCTTCATGAGGAAGATGCTCGCCGCCGTCTCCCGCGTGCTGT
  CTGGCGCTTCTCAGAAGCCGGCAAGCAGAGTGCTGGTAGCATCCCGTAATTTTGCAAATG
  ATGCTACATTTGAAATTAAGAAATGTGACCTTCACCGGCTGGAAGAAGGCCCTCCTGTCA
  CAACAGTGCTCACCAGGGAGGATGGGCTCAAATACTACAGGATGATGCAGACTGTACGCC
  GAATGGAGTTGAAAGCAGATCAGCTGTATAAACAGAAAATTATTCGTGGTTTCTGTCACT
  TGTGTGATGGTCAGGAAGCTTGCTGTGTGGGCCTGGAGGCCGGCATCAACCCCACAGACC
  ATCTCATCACAGCCTACCGGGCTCACGGCTTTACTTTCACCCGGGGCCTTTCCGTCCGAG
  AAATTCTCGCAGAGCTTACAGGACGAAAAGGAGGTTGTGCTAAAGGGAAAGGAGGATCGA
  TGCACATGTATGCCAAGAACTTCTACGGNGGCAATGGCATCGTGGGAGCGCAGGTGCCCC
  TGNGCGCTGGGATTGCTCTAGCCCTGTAGTATAATGGAAAAGATGAGGTCTGCCTGACTN
  TATATGGCGATGGTGCTGCTAACCAGNGCCAGATATTCGAAGCTTACAACATGGCAGCCT
  TTGTGGAAATTACCTTGTATTNTCATCTGTGAGAATAATCGCTATGGAATGGGAACGTCT
  GTTGAGAGAGCGGCAGCCAGCACTGATTACTANCAGAGAGGGCGATTCATTCCTGGGCTG
  AGAGTGGATGGAATGGATATCCTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000284 unedited
  TTGGACCGCGGCCGCTTCTATAGTCGGTTTTTTTTTTTTTTTTTTAATCTTTAAGCACTC
  AATAATTCATCTTTTAATGCACTAATGTACAAACTGCATGCAATTACTACCTGCTCAATC
  TACACACTTAATGGAAGTTTCCAAGAATTTCATTGAATTTCATTGACTGGGTTTTCTTCC
  TCCTTAACCAAGTTGAGAACACTGTCTGGTAGCCCCCTGAAGGTATAACCTCTCCTTCTC
  CTCCCCTTAACTGACTGACTTAAACTTGATCCACTGATTGGCACCACGAACTTCAAAAGG
  TGGGTCGCTGGAGTAGATGTGGTAGCCCAGCTCTTCCAAAGGTGGCTCAGGATCGGCCGT
  GGCAAACTGGGCAGCATCCTCAATCTCCTTCCTCACTTCCACATCAATTTCCTTTAGTTC
  TTCCACACTGGCAAGATTGCTGTTCACCATCCTGTCCTTGAGAAGCATAATAAGGTCACT
  CTTACTTCTTACTTCCTGAATTTCTTCTCGTGTACGGTAACTGACTCCAGGGTCACTCAT
  ACTGTGTCCGTGGGAACGGTAAGTCTGCAACTCCATAAGAATGGCCCCTTTTCCCAATTT
  ACATTGGGCGCCAGCAAACCTTTGTTGCCCTTTGGGCCCCCAAGGAATCCTTTCCTTCCC
  CTTTAAACCCAAAAAGAAAAACCCCTCCTTTGTGGAAAAAAAGGGGGGGGGGGCGCTTTT
  TTAAAAAAAATGTCCCCTTCTTATAAGATTTTTTTCCCCAATAAAAAAAAAGGGGGTTTT
  CCCCCAACGCCCCGCCGGGGGAGGCCTAAAATTTTGGGCCGGGGTGGAACCCCCCCCCCC
  TCTTTTTTAATGAGGGGGACCATTTTTTTTTTTTTTTTTTTTTGGGCGGAGAGAAAAANC
  CCCCCGGGGGGGGGGGCGGTCTCTCCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000284
• Insert Size: 1460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000284.1, NP_000275.1
• RefSeq Size: 1472 bp
• RefSeq ORF: 1173 bp
• Locus ID: 5160
• Cytogenetics: Xp22.12
• Domains: E1_dehydrog
• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine biosynthesis
• Gene Summary: 'The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]'
  Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1.