PGK1 (NM_000291) Human Untagged Clone

CAT#: SC119988

PGK1 (untagged)-Human phosphoglycerate kinase 1 (PGK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PGK1
• Synonyms: HEL-S-68p; MIG10; PGKA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119988 sequence for NM_000291 edited (data generated by NextGen Sequencing)
  ATGTCGCTTTCTAACAAGCTGACGCTGGACAAGCTGGACGTTAAAGGGAAGCGGGTCGTT
  ATGAGAGTCGACTTCAATGTTCCTATGAAGAACAACCAGATAACAAACAACCAGAGGATT
  AAGGCTGCTGTCCCAAGCATCAAATTCTGCTTGGACAATGGAGCCAAGTCGGTAGTCCTT
  ATGAGCCACCTAGGCCGGCCTGATGGTGTGCCCATGCCTGACAAGTACTCCTTAGAGCCA
  GTTGCTGTAGAACTCAAATCTCTGCTGGGCAAGGATGTTCTGTTCTTGAAGGACTGTGTA
  GGCCCAGAAGTGGAGAAAGCCTGTGCCAACCCAGCTGCTGGGTCTGTCATCCTGCTGGAG
  AACCTCCGCTTTCATGTGGAGGAAGAAGGGAAGGGAAAAGATGCTTCTGGGAACAAGGTT
  AAAGCCGAGCCAGCCAAAATAGAAGCTTTCCGAGCTTCACTTTCCAAGCTAGGGGATGTC
  TATGTCAATGATGCTTTTGGCACTGCTCACAGAGCCCACAGCTCCATGGTAGGAGTCAAT
  CTGCCACAGAAGGCTGGTGGGTTTTTGATGAAGAAGGAGCTGAACTACTTTGCAAAGGCC
  TTGGAGAGCCCAGAGCGACCCTTCCTGGCCATCCTGGGCGGAGCTAAAGTTGCAGACAAG
  ATCCAGCTCATCAATAATATGCTGGACAAAGTCAATGAGATGATTATTGGTGGTGGAATG
  GCTTTTACCTTCCTTAAGGTGCTCAACAACATGGAGATTGGCACTTCTCTGTTTGATGAA
  GAGGGAGCCAAGATTGTCAAAGACCTAATGTCCAAAGCTGAGAAGAATGGTGTGAAGATT
  ACCTTGCCTGTTGACTTTGTCACTGCTGACAAGTTTGATGAGAATGCCAAGACTGGCCAA
  GCCACTGTGGCTTCTGGCATACCTGCTGGCTGGATGGGCTTGGACTGTGGTCCTGAAAGC
  AGCAAGAAGTATGCTGAGGCTGTCACTCGGGCTAAGCAGATTGTGTGGAATGGTCCTGTG
  GGGGTATTTGAATGGGAAGCTTTTGCCCGGGGAACCAAAGCTCTCATGGATGAGGTGGTG
  AAAGCCACTTCTAGGGGCTGCATCACCATCATAGGTGGTGGAGACACTGCCACTTGCTGT
  GCCAAATGGAACACGGAGGATAAAGTCAGCCATGTGAGCACTGGGGGTGGTGCCAGTTTG
  GAGCTCCTGGAAGGTAAAGTCCTTCCTGGGGTGGATGCTCTCAGCAATATTTAG
  
  Clone variation with respect to NM_000291.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000291 unedited
  GTTAGGAATTGTATCCGACTCCTATAGGCGGCCGCGCAATTCGCACGAGGCCGCATTCTG
  CAAGCCTCCGGAGCGCACGTCGGCAGTCGGCTCCCTCGTTGACCGAATCACCGACCTCTC
  TCCCCAGCTGTATTTCCAAAATGTCGCTTTCTAACAAGCTGACGCTGGACAAGCTGGACG
  TTAAAGGGAAGCGGGTCGTTATGAGAGTCGACTTCAATGTTCCTATGAAGAACAACCAGA
  TAACAAACAACCAGAGGATTAAGGCTGCTGTCCCAAGCATCAAATTCTGCTTGGACAATG
  GAGCCAAGTCGGTAGTCCTTATGAGCCACCTAGGCCGGCCTGATGGTGTGCCCATGCCTG
  ACAAGTACTCCTTAGAGCCAGTTGCTGTAGAACTCAAATCTCTGCTGGGCAAGGATGTTC
  TGTTCTTGAAGGACTGTGTAGGCCCAGAAGTGGAGAAAGCCTGTCCCAACCCAGCTGCNT
  GGGTCTGTCATCCTGCTGGAGAACCTCCGCTTTCATGTGGAGGAAGAAGGGAAGGGAAAA
  GATGCTTCTGGGAACAAGGTTAAAGCCGAGCCAGCCAAAATAGAAGCTTTCCGAGCTTCA
  CTTTCCAAGCNTAGGGGATGTCTATGTCAATGATGCTTTTGGCACTGCTCACAGAGCCCA
  CAGCTCCATGGTAGGAGTCAATCTGCCACAGAAGGCTGGTGGGTTNTTGATGAAGAAGGA
  GCTGAACTACTTTGNCAAGGCCTTGGAGAGCCCAGAGCGACCCTTCCTGGCCATCCTGNG
  CGGAGCTAAAGNTGCAGACCAGATCCAGCTCATCAATAATATGCTGGACAAAGTCAATGA
  GATGATTATTGGTGGNTGGAAATGGCTTTACCTTCCTTAANNGTGCTCACACATGGAGAT
  GCCCTTTNCCTTGTTTGATGAAAAGGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000291 unedited
  ATGGCACTTCCAGGGCCAGGTANAGCACTGGTGGNAGGGGTCACAGGGNATGCCCACCCG
  GGTATCTGGTTCAGGNAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTT
  TTTTTTTTTTCACGGTTTCAATGGACACTTTTATTGTTTACTTAATGGATCATCAATTTT
  GTCTCACTACCTACAAATGGAATTTCATCTTGTTTCCATGCTGAGTAGTGAAACAGTGAC
  AAAGCTAATCATAATAACCTACATCAAAAGAGAACTAAGCTAACACTGCTCACTTTCTTT
  TTAACAGGCAAAATATAAATATATGCACTCTAGAATGCACAATGGTTTATTCACTAAAAA
  ATTCAAATGGGATCTTGAAGAATGTATGCAAATCCAGGGTGCAGAGAAAATGAGCTGATA
  TGCTGTGCAACTGTTTAAGGGTTCCTGGCACTGCATCTCTTGGCCACTAGCTGAATCTTG
  ACATGGAAGGTTTTAGCTAATGCCAAGTGGAGATGCAGAAAATGCTAAGTTGACTTAGGG
  GCTGTGCACAGGAACTAAAAGGCAGGAAAGTACTAAATATTGCTGAGAGCATCCACCCCA
  GGAAGGACTTTACCTTCCAGGAGCTCCAAACTGGCACCACCCCCAGTGCTCACATGGCTG
  ACTTTATCCTCCGTGTTCCATTTGGCACAGCAAGTGGCAGTGTCTCCACCACCTATGATG
  GTGATGCAGCCCCTANAAGTGGCTTTTCACACCTCATCCATGAGAAGCTTTGGTTCCCCG
  GGCAAAAGCTTTCCATTCAAATACCCNNCACAGGACCATTCCACACAAATCTGCTTAGNC
  CCCGATGACAGNCCTNAGCATACTTTCTTGCTGCTTTTCAGAACCACAAGTCCAAGCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000291
• Insert Size: 1910 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000291.2, NP_000282.1
• RefSeq Size: 2338 bp
• RefSeq ORF: 1254 bp
• Locus ID: 5230
• Cytogenetics: Xq21.1
• Domains: PGK
• Protein Families: Druggable Genome
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways
• Gene Summary: 'The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]'