MLH1 (NM_000249) Human Untagged Clone

CAT#: SC119993

MLH1 (untagged)-Human mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) (MLH1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MLH1
• Synonyms: COCA2; FCC2; hMLH1; HNPCC; HNPCC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119993 sequence for NM_000249 edited (data generated by NextGen Sequencing)
  ATGTCGTTCGTGGCAGGGGTTATTCGGCGGCTGGACGAGACAGTGGTGAACCGCATCGCG
  GCGGGGGAAGTTATCCAGCGGCCAGCTAATGCTATCAAAGAGATGATTGAGAACTGTTTA
  GATGCAAAATCCACAAGTATTCAAGTGATTGTTAAAGAGGGAGGCCTGAAGTTGATTCAG
  ATCCAAGACAATGGCACCGGGATCAGGAAAGAAGATCTGGATATTGTATGTGAAAGGTTC
  ACTACTAGTAAACTGCAGTCCTTTGAGGATTTAGCCAGTATTTCTACCTATGGCTTTCGA
  GGTGAGGCTTTGGCCAGCATAAGCCATGTGGCTCATGTTACTATTACAACGAAAACAGCT
  GATGGAAAGTGTGCATACAGAGCAAGTTACTCAGATGGAAAACTGAAAGCCCCTCCTAAA
  CCATGTGCTGGCAATCAAGGGACCCAGATCACGGTGGAGGACCTTTTTTACAACATAGCC
  ACGAGGAGAAAAGCTTTAAAAAATCCAAGTGAAGAATATGGGAAAATTTTGGAAGTTGTT
  GGCAGGTATTCAGTACACAATGCAGGCATTAGTTTCTCAGTTAAAAAACAAGGAGAGACA
  GTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGACAATATTCGCTCCGTCTTT
  GGAAATGCTGTTAGTCGAGAACTGATAGAAATTGGATGTGAGGATAAAACCCTAGCCTTC
  AAAATGAATGGTTACATATCCAATGCAAACTACTCAGTGAAGAAGTGCATCTTCTTACTC
  TTCATCAACCATCGTCTGGTAGAATCAACTTCCTTGAGAAAAGCCATAGAAACAGTGTAT
  GCAGCCTATTTGCCCAAAAACACACACCCATTCCTGTACCTCAGTTTAGAAATCAGTCCC
  CAGAATGTGGATGTTAATGTGCACCCCACAAAGCATGAAGTTCACTTCCTGCACGAGGAG
  AGCATCCTGGAGCGGGTGCAGCAGCACATCGAGAGCAAGCTCCTGGGCTCCAATTCCTCC
  AGGATGTACTTCACCCAGACTTTGCTACCAGGACTTGCTGGCCCCTCTGGGGAGATGGTT
  AAATCCACAACAAGTCTGACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCC
  CACCAGATGGTTCGTACAGATTCCCGGGAACAGAAGCTTGATGCATTTCTGCAGCCTCTG
  AGCAAACCCCTGTCCAGTCAGCCCCAGGCCATTGTCACAGAGGATAAGACAGATATTTCT
  AGTGGCAGGGCTAGGCAGCAAGATGAGGAGATGCTTGAACTCCCAGCCCCTGCTGAAGTG
  GCTGCCAAAAATCAGAGCTTGGAGGGGGATACAACAAAGGGGACTTCAGAAATGTCAGAG
  AAGAGAGGACCTACTTCCAGCAACCCCAGAAAGAGACATCGGGAAGATTCTGATGTGGAA
  ATGGTGGAAGATGATTCCCGAAAGGAAATGACTGCAGCTTGTACCCCCCGGAGAAGGATC
  ATTAACCTCACTAGTGTTTTGAGTCTCCAGGAAGAAATTAATGAGCAGGGACATGAGGTT
  CTCCGGGAGATGTTGCATAACCACTCCTTCGTGGGCTGTGTGAATCCTCAGTGGGCCTTG
  GCACAGCATCAAACCAAGTTATACCTTCTCAACACCACCAAGCTTAGTGAAGAACTGTTC
  TACCAGATACTCATTTATGATTTTGCCAATTTTGGTGTTCTCAGGTTATCGGAGCCAGCA
  CCGCTCTTTGACCTTGCCATGCTTGCCTTAGATAGTCCAGAGAGTGGCTGGACAGAGGAA
  GATGGTCCCAAAGAAGGACTTGCTGAATACATTGTTGAGTTTCTGAAGAAGAAGGCTGAG
  ATGCTTGCAGACTATTTCTCTTTGGAAATTGATGAGGAAGGGAACCTGATTGGATTACCC
  CTTCTGATTGACAACTATGTGCCCCCTTTGGAGGGACTGCCTATCTTCATTCTTCGACTA
  GCCACTGAGGTGAATTGGGACGAAGAAAAGGAATGTTTTGAAAGCCTCAGTAAAGAATGC
  GCTATGTTCTATTCCATCCGGAAGCAGTACATATCTGAGGAGTCGACCCTCTCAGGCCAG
  CAGAGTGAAGTGCCTGGCTCCATTCCAAACTCCTGGAAGTGGACTGTGGAACACATTGTC
  TATAAAGCCTTGCGCTCACACATTCTGCCTCCTAAACATTTCACAGAAGATGGAAATATC
  CTGCAGCTTGCTAACCTGCCTGATCTATACAAAGTCTTTGAGAGGTGTTAA
  
  Clone variation with respect to NM_000249.3
  655 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000249 unedited
  GACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCAAATGTCGTTCGTGGCAGGG
  GTTATTCGGCGGCTGGACGAGACAGTGGTGAACCGCATCGCGGCGGGGGAAGTTATCCAG
  CGGCCAGCTAATGCTATCAAAGAGATGATTGAGAACTGTTTAGATGCAAAATCCACAAGT
  ATTCAAGTGATTGTTAAAGAGGGAGGCCTGAAGTTGATTCAGATCCAAGACAATGGCACC
  GGGATCAGGAAAGAAGATCTGGATATTGTATGTGAAAGGTTCACTACTAGTAAACTGCAG
  TCCTTTGAGGATTTAGCCAGTATTTCTACCTATGGCTTTCGAGGTGAGGCTTTGGCCAGC
  ATAAGCCATGTGGCTCATGTTACTATTACAACGAAAACAGCTGATGGAAAGTGTGCATAC
  AGAGCAAGTTACTCAGATGGAAAACTGAAAGCCCCTCCTAAACCATGTGCTGGCAATCAA
  GGGACCCAGATCACGGTGGAGGACCTTTTTTACAACATAGCCACGAGGAGAAAAGCTNTA
  AAAAATCCAAGTGAAGAATATGGGAAAATTTTGGAAGTTGTTGGCAGGTATTCAGTACAC
  AATGCANGCATTAGTTTCTCAGTTAANAAACAAGGAGAGACAGTAGCTGATGTTANGACA
  CTACCNCATGCCTNCACCGTGGGACATATTCGCTCCGTCTTTGGAAATGCTGNTAGTCGA
  GAACTGATAGAAATGGNATGTGANNGATAAACCCNTAGCCTTCAAATGAATGGNTACATA
  TCCATGCANACTACTCAGTGAAGAAGTGCATCTTTCTACTCTTCATCACCATCGTCTGGT
  AGAATCACTTNCTTGAAAAAGCATAGAACAGGTATGCAGCCTATTGCCAAAACACCACCC
  ATTCTGTACTCATTAGAATCATCCCAGATGTGATGTATGTGCCCCACAAGCTGAGTCACT
  TCTGACAGAGACTCTGGACGGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000249 unedited
  ACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATGTTAAGACACATCTATTTATTT
  ATAATCAATCCACTGTGTATAAAGGAATACTATCAGAAGGCAAGTATAAGTCTTAAGTGC
  TACCAACACTTATGTTGGTACACTTTGTATATCACACTTTGATACAACACTTTGTATCGG
  AATACAGAGAAAGAAGAACACATCCCACAGTGCATAAATAACCATATTTAACACCTCTCA
  AAGACTTTGTATAGATCAGGCAGGTTAGCAAGCTGCAGGATATTTCCATCTTCTGTGAAA
  TGTTTAAGAGGCAGAATGTGTGAGCGCAAGGCTTTATAGACAATGTGTTCCACAGTCCAC
  TTCCAGGAGTTTGGAATGGAGCCAGGCACTTCACTCTGCTGGCCTGAGAGGGTCGACTCC
  TCAGATATGTACTGCTTCCGGATGGAATAGAACATAGCGCATTCTTTACTGAGGCTTTCA
  AAACATTCCTTTTCTTCGTCCCAATTCACCTCAGTGGCTAGTCGAAGAATGAATATAGGC
  AGTCCCTCCAAAGGGGGCACATAGTTGTCAATCAGAAGGGGTAATCCAATCAGGTTCCCT
  TCCTCATCAATTTCCAAAGAAGAATAGTCTGCAAGCCTCCCAACCTTCTTCTTCAGAAAC
  TAACAATGTATTCACCAAGTCCTTCTTTTGGACCATCTTCCTCTGGCCAGCCACTCCTTG
  GATTTTTAAGGCACCCTGGCAGGGCAAAAAGCGAGCTGGCTCCGTTACCCGAGAACCCAA
  ATCGGGAAATCCTAATGACCTCGGCACAAACCCCTCCCCACCTGGGGGGGTGAAAAGAAT
  ACTGGTTCAGCTGTGCCAGGCCCCTGGTTCACCTCCCCGAGGTGGGTTTACACTTTTCGA
  AAACTAGTACCCGCCATTCTTTCCCGGCACTCTCCCCTCGGGCTTTGTCTCCCGTGGGCG
  CATCTT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_000249
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000249.2, NP_000240.1
• RefSeq Size: 2524 bp
• RefSeq ORF: 2271 bp
• Locus ID: 4292
• Cytogenetics: 3p22.2
• Domains: DNA_mis_repair, HATPase_c
• Protein Families: Druggable Genome
• Protein Pathways: Colorectal cancer, Endometrial cancer, Mismatch repair, Pathways in cancer
• Gene Summary: 'The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).