Myeloperoxidase (MPO) (NM_000250) Human Untagged Clone

CAT#: SC119994

MPO (untagged)-Human myeloperoxidase (MPO), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MPO
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119994 sequence for NM_000250 edited (data generated by NextGen Sequencing)
  ATGGGGGTTCCCTTCTTCTCTTCTCTCAGATGCATGGTGGACTTAGGACCTTGCTGGGCT
  GGGGGTCTCACTGCAGAGATGAAGCTGCTTCTGGCCCTAGCAGGGCTCCTGGCCATTCTG
  GCCACGCCCCAGCCCTCTGAAGGTGCTGCTCCAGCTGTCCTGGGGGAGGTGGACACCTCG
  TTGGTGCTGAGCTCCATGGAGGAGGCCAAGCAGCTGGTGGACAAGGCCTACAAGGAGCGG
  CGGGAAAGCATCAAGCAGCGGCTTCGCAGCGGCTCAGCCAGCCCCATGGAACTCCTATCC
  TACTTCAAGCAGCCGGTGGCAGCCACCAGGACGGCGGTGAGGGCCGCTGACTACCTGCAC
  GTGGCTCTAGACCTGCTGGAGAGGAAGCTGCGGTCCCTGTGGCGAAGGCCATTCAATGTC
  ACTGATGTGCTGACGCCCGCCCAGCTGAATGTGTTGTCCAAGTCAAGCGGCTGCGCCTAC
  CAGGACGTGGGGGTGACTTGCCCGGAGCAGGACAAATACCGCACCATCACCGGGATGTGC
  AACAACAGACGCAGCCCCACGCTGGGGGCCTCCAACCGTGCCTTTGTGCGCTGGCTGCCG
  GCGGAGTATGAGGACGGCTTCTCTCTTCCCTACGGCTGGACGCCCGGGGTCAAGCGCAAC
  GGCTTCCCGGTGGCTCTGGCTCGCGCGGTCTCCAACGAGATCGTGCGCTTCCCCACTGAT
  CAGCTGACTCCGGACCAGGAGCGCTCACTCATGTTCATGCAATGGGGCCAGCTGTTGGAC
  CACGACCTCGACTTCACCCCTGAGCCGGCCGCCCGGGCCTCCTTCGTCACTGGCGTCAAC
  TGCGAGACCAGCTGCGTTCAGCAGCCGCCCTGCTTCCCGCTCAAGATCCCGCCCAATGAC
  CCCCGCATCAAGAACCAAGCCGACTGCATCCCGTTCTTCCGCTCCTGCCCGGCTTGCCCC
  GGGAGCAACATCACCATCCGCAACCAGATCAACGCGCTCACTTCCTTCGTGGACGCCAGC
  ATGGTGTACGGCAGCGAGGAGCCCCTGGCCAGGAACCTGCGCAACATGTCCAACCAGCTG
  GGGCTGCTGGCCGTCAACCAGCGCTTCCAAGACAACGGCCGGGCCCTGCTGCCCTTTGAC
  AACCTGCACGATGACCCCTGTCTCCTCACCAACCGCTCAGCGCGCATCCCCTGCTTCCTG
  GCAGGGGACACCCGTTCCAGTGAGATGCCCGAGCTCACCTCCATGCACACCCTCTTACTT
  CGGGAGCACAACCGGCTGGCCACAGAGCTCAAGAGCCTGAACCCTAGGTGGGATGGGGAG
  AGGCTCTACCAGGAAGCCCGGAAGATCGTGGGGGCCATGGTCCAGATCATCACTTACCGG
  GACTACCTGCCCCTGGTGCTGGGGCCAACGGCCATGAGGAAGTACCTGCCCACGTACCGT
  TCCTACAATGACTCAGTGGACCCACGCATCGCCAACGTCTTCACCAATGCCTTCCGCTAC
  GGCCACACCCTCATCCAACCCTTCATGTTCCGCCTGGACAATCGGTACCAGCCCATGGAA
  CCCAACCCCCGTGTCCCCCTCAGCAGGGTCTTTTTTGCCTCCTGGAGGGTCGTGCTGGAA
  GGTGGCATTGACCCCATCCTCCGGGGCCTCATGGCCACCCCTGCCAAGCTGAATCGTCAG
  AACCAAATTGCAGTGGATGAGATCCGGGAGCGATTGTTTGAGCAGGTCATGAGGATTGGG
  CTGGACCTGCCTGCTCTGAACATGCAGCGCAGCAGGGACCACGGCCTCCCAGGATACAAT
  GCCTGGAGGCGCTTCTGTGGGCTCCCGCAGCCTGAAACTGTGGGCCAGCTGGGCACGGTG
  CTGAGGAACCTGAAATTGGCGAGGAAACTGATGGAGCAGTATGGCACGCCCAACAACATC
  GACATCTGGATGGGCGGCGTGTCCGAGCCTCTGAAGCGCAAAGGCCGCGTGGGCCCACTC
  CTCGCCTGCATCATCGGTACCCAGTTCAGGAAGCTCCGGGATGGTGATCGGTTTTGGTGG
  GAGAACGAGGGTGTGTTCAGCATGCAGCAGCGACAGGCCCTGGCCCAGATCTCATTGCCC
  CGGATCATCTGCGACAACACAGGCATCACCACCGTGTCTAAGAACAACATCTTCATGTCC
  AACTCATATCCCCGGGACTTTGTCAACTGCAGTACACTTCCTGCATTGAACCTGGCTTCC
  TGGAGGGAAGCCTCCTAG
  
  Clone variation with respect to NM_000250.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000250 unedited
  TAAGTTTAAATATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACCAGGTCCTT
  GGAAGCTGGATGACAGCAGCTGGCAAGGGGATAAGAGAGCAGTGAGCCCCTCCCTCAAGA
  GGTCTGGCTTTATCCATAGACAGGGCCCTCTGAGGTGGGGCTGAGGTACAAAGGGGGATT
  GAGCAGCCCAGGAGAAGAGAGATGGGGGTTCCCTTCTTCTCTTCTCTCAGATGCATGGTG
  GACTTAGGACCTTGCTGGGCTGGGGGTCTCACTGCAGAGATGAAGCTGCTTCTGGCCCTA
  GCAGGGCTCCTGGCCATTCTGGCCACGCCCCAGCCCTCTGAAGGTGCTGCTCCAGCTGTC
  CTGGGGGAGGTGGACACCTCGTTGGTGCTGAGCTCCATGGAGGAGGCCAAGCAGCTGGTG
  GACAAGGCCTACAAGGAGCGGCGGGAAAGCATCAAGCAGCGGCTTCGCAGCGGCTCAGCC
  AGCCCCATGGAACTCCTATCCTACTTCAAGCAGCCGGTGGCAGCCACCAGGACGGCGGTG
  AGGGCCGCTGACTACCTGCACGTGGCTCTAGACCTGCTGGAGAGGAAGCTGCGGTCCCTG
  TGGCGAAGGCCATTCAATGTCACTGATGTGCTGACGCCCGCCCAGCTGAATGTGTTGTCC
  AAGTCAAGCGGCTGCGCCTACCAGGACGTGGGGGTGACTTGCCCGGAGCANGACAAATAC
  CGCACCATCACCGGNGATGTGCAACAACAGACGCAGCCCCACGCTGGGGGCCTCCAACCG
  TGCCTTTGTGCGCTGGCTGCNCGCNGAGTATGANGACGGCTTCTCTCTTCCCTACNGCTG
  GACGCCCGGGGTCAAGCGCAAACGCTTACCCGTGGCTCTGGCTCGCGCGGTCTCCACCNA
  NATCGTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000250 unedited
  AAAAGGGGAAAANNACANNNCNNCTTTCCCCCCCTNTTTTTACTTAGNNACGCGGNCCGC
  ATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTCAGCAAACACACACGCATGAAAAAGCC
  AATTTATATACTTCGCACATACATGAGCACACAAATGAACGTCTAGTCACTCATTTTCTC
  AGCTGCACCCAAACAGGGCTGGGCTCATCTAGGGCAAGGAGATCTCCGTGGTTCCAACTG
  GCCAGCCCAGATATACCCCTCACTGCTGCACCCCCTTACCTGGCCTCTAGGAGGCTTCCC
  TCCAGGAAGCCAGGTTCAATGCAGGAAGTGTACTGCAGTTGACAAAGTCCCGGGGATATG
  AGTTGGACATGAAGATGTTGTTCTTAGACACGGTGGTGATGCCTGTGTTGTCGCAGATGA
  TCCGGGGCAATGAGATCTGGGCCAGGGCCTGTCGCTGCTGCATGCTGAACACACCCTCGT
  TCTCCCACCAAAACCGATCACCATCCCGGAGCTTCCTGAACTGGGTACCGATGATGCAGG
  CGAGGAGTGGGCCCACGCGGCCTTTGCGCTTCAGAGGCTCGGACACGCCGCCCATCCAGA
  TGTCGATGTTGTTGGGCGTGCCATACTGCTCCATCAGTTTCCTCGCCAATTTCAGGTTCC
  TCAGCACCGTGCCCAGCTGGCCCACAGTTTCAGGCTGCGGGAGCCCACAGAAGCGCCTCC
  AGGCATTGTATCCTGGGAGGCCGTGGTCCCTGCTGCGCTGCATGTTCAAAGCAAGCAGGT
  CCAGCCCAATCCTATGACCTGCTCAACAATCGCTCCCGGACTCATCCCTGCAATTTGGTT
  CTGACCATTCACCTGGCAGGGTGGGCATGAGCCCCCGAAGAAGGGTCAATGCCACCTTCA
  GACGACCCTCAGAAGCAAAAAAACCCTCTTAGGGGAACACGGGGTTGGGTCCATGGCTTG
  TACCCATGTCCAGCCGCACATGAGGTTGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000250
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000250.1, NP_000241.1
• RefSeq Size: 3215 bp
• RefSeq ORF: 2238 bp
• Locus ID: 4353
• Cytogenetics: 17q22
• Domains: An_peroxidase
• Protein Families: Druggable Genome
• Gene Summary: 'Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]'