Caspase 8 (CASP8) (NM_033358) Human Untagged Clone

CAT#: SC119997

CASP8 (untagged)-Human caspase 8, apoptosis-related cysteine peptidase (CASP8), transcript variant E

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CASP8
• Synonyms: ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_033358 edited
  GAATTCGGCACGAGGGATAGGCCTGTGACGAAGGTGCTACCATCGTGAGAGTAAGATTAT
  ATTCTCCTGCCTTTTAAAAAGATGGACTTCAGCAGAAATCTTTATGATATTGGGGAACAA
  CTGGACAGTGAAGATCTGGCCTCCCTCAAGTTCCTGAGCCTGGACTACATTCCGCAAAGG
  AAGCAAGAACCCATCAAGGATGCCTTGATGTTATTCCAGAGACTCCAGGAAAAGAGAATG
  TTGGAGGAAAGCAATCTGTCCTTCCTGAAGGAGCTGCTCTTCCGAATTAATAGACTGGAT
  TTGCTGATTACCTACCTAAACACTAGAAAGGAGGAGATGGAAAGGGAACTTCAGACACCA
  GGCAGGGCTCAAATTTCTGCCTACAGGGTCATGCTCTATCAGATTTCAGAAGAAGTGAGC
  AGATCAGAATTGAGGTCTTTTAAGTTTCTTTTGCAAGAGGAAATCTCCAAATGCAAACTG
  GATGATGACATGAACCTGCTGGATATTTTCATAGAGATGGAGAAGAGGGTCATCCTGGGA
  GAAGGAAAGTTGGACATCCTGAAAAGAGTCTGTGCCCAAATCAACAAGAGCCTGCTGAAG
  ATAATCAACGACTATGAAGAATTCAGCAAAGAGAGAAGCAGCAGCCTTGAAGGAAGTCCT
  GATGAATTTTCAAATGGGGAGGAGTTGTGTGGGGTAATGACAATCTCGGACTCTCCAAGA
  GAACAGGATAGTGAATCACAGACTTTGGACAAAGTTTACCAAATGAAAAGCAAACCTCGG
  GGATACTGTCTGATCATCAACAATCACAATTTTGCAAAAGCACGGGAGAAAGTGCCCAAA
  CTTCACAGCATTAGGGACAGGAATGGAACACACTTGGATGCAGGGGCTTTGACCACGACC
  TTTGAAGAGCTTCATTTTGAGATCAAGCCCCACGATGACTGCACAGTAGAGCAAATCTAT
  GAGATTTTGAAAATCTACCAACTCATGGACCACAGTAACATGGACTGCTTCATCTGCTGT
  ATCCTCTCCCATGGAGACAAGGGCATCATCTATGGCACTGATGGACAGGAGGCCCCCATC
  TATGAGCTGACATCTCAGTTCACTGGTTTGAAGTGCCCTTCCCTTGCTGGAAAACCCAAA
  GTGTTTTTTATTCAGGCTTGTCAGGGGGATAACTACCAGAAAGGTATACCTGTTGAGACT
  GATTCAGAGGAGCAACCCTATTTAGAAATGGATTTATCATCACCTCAAACGAGATATATC
  CCGGATGAGGCTGACTTTCTGCTGGGGATGGCCACTGTGAATAACTGTGTTTCCTACCGA
  AACCCTGCAGAGGGAACCTGGTACATCCAGTCACTTTGCCAGAGCCTGAGAGAGCGATGT
  CCTCGAGGCGATGATATTCTCACCATCCTGACTGAAGTGAACTATGAAGTAAGCAACAAG
  GATGACAAGAAAAACATGGGGAAACAGATGCCTCAGCCTACTTTCACACTAAGAAAAAAA
  CTTGTCTTCCCTTCTGATTGATGGTGCTATTTTGTTTGTTTTGTTTTGTTTTGTTTTTTT
  GAGACAGAATCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACCG
  CAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGAC
  TACAGGGGCCCGCCACCACACCTGGCTAATTTTTTAAAAATATTTTTAGTAGAGACAGGG
  TTTCACTGTGTTAGCCAGGGTGGTCTTGATCTCCTGACCTCGTGATCCACCCACCTCGGC
  CTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCTGGCCGATGGTACTATTTAG
  ATATAACACTATGTTTATTTACTAATTTTCTAGATTTTCTACTTTATTAATTGTTTTGCA
  CTTTTTTATAAGAGCTAAAGTTAAATAGGATATTAACAACAATAACACTGTCTCCTTTCT
  CTTATGCTTAAGGCTTTGGGAATGTTTTTAGCTGGTGGCAATAAATACCAGACACGTACA
  AAATCCAGCTATGAATATAGAGGGCTTATGATTCAGATTGTTATCTATCAACTATAAGCC
  CACTGTTAATATTCTATTAACTTTAATTCTCTTTCAAAGCTAAATTCCACACTACCACAT
  TAAAAAAATTAGAAAGTAGCCACGTATGGTGGCTCATGTCTATAATCCCAGCACTTTGGG
  AGGTTGAGGTGGGAGGATTGCTTGAACCCAAGAGGTCAAGGCTGCAGTGAGCCATGTTCA
  CACCGCTGCACTCAAGCTTGGGTGACAGAGCAAGACCCCGTCTCAAAAAAAATTTTTTTT
  TTAATAAAACAAAATTTGTTTGAAAACTTTTAAAAATTCAAATGATTTTTACAAGTTTTA
  AATAAGCTCTCCCCAAACTTGCTTTATGCCTTCTTATTGCTTTTATGATATATATATGCT
  TGGCTAACTATATTTGCTTTTTGCTAACAATGCTCTGGGGTCTTTTTATGCATTTGCATT
  TGCTCTTTCATCTCTGCTTGGATTATTTTAAATCATTAGGAATTAAGTTATCTTTAAAAT
  TTAAGTATCTTTTTTCAAAAACATTTTTTAATAGAATAAAATATAATTTGATCTTAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033358 unedited
  TCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGGATAGGC
  CTGTGACGAAGGTGCTACCATCGTGAGAGTAAGATTATATTCTCCTGCCTTTTAAAAAGA
  TGGACTTCAGCAGAAATCTTTATGATATTGGGGAACAACTGGACAGTGAAGATCTGGCCT
  CCCTCAAGTTCCTGAGCCTGGACTACATTCCGCAAAGGAAGCAAGAACCCATCAAGGATG
  CCTTGATGTTATTCCAGAGACTCCAGGAAAAGAGAATGTTGGAGGAAAGCAATCTGTCCT
  TCCTGAAGGAGCTGCTCTTCCGAATTAATAGACTGGATTTGCTGATTACCTACCTAAACA
  CTAGAAAGGAGGAGATGGAAAGGGAACTTCAGACACCAGGCAGGGCTCAAATTTCTGCCT
  ACAGGGTCATGCTCTATCAGATTTCAGAAGAAGTGAGCAGATCAGAATTGAGGTCTTTTA
  AGTTTCTTTTGCAAGAGGAAATCTCCAAATGCAAACTGGATGATGACATGAACCTGCTGG
  ATATTTTCATAGAGATGGAGAAGAGGGTCATCCTGNGAGAAGGAAAGTTGGACATCCTGA
  AAAGAGTCTGTGCCCAAATCAACAAGAGCCTGCTGAAGATAATCAACGACTATGAAGAAT
  TCAGCAAAGAGAGAAGCAGCAGCCTTGAAGGAAGTCCTGATGAATTTTCAAATGGGGAGG
  AGTTGTGTGGGGTAATGACAATCTCGGACTCTTCAAGAGAACAGGATAGTGAATCACAGA
  CTTTGGACANNAGTTACCAAATGAAAAGCANACCTCGNGATACTGTCTGATCATAACATC
  ACATTNTGCAAAGCACGGAGAAATGCCCAACTCACGCATAGGACAGGATGGACAACTNGA
  TGCAGGCTTGCCCGACTTGAGAGCTATTTGAATAGCCCACATACTGACATNACAATTTGA
  A
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033358
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033358.1, NP_203522.1
• RefSeq Size: 1088 bp
• RefSeq ORF: 708 bp
• Locus ID: 841
• Cytogenetics: 2q33.1
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease, Apoptosis, Huntington's disease, NOD-like receptor signaling pathway, p53 signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Viral myocarditis
• Gene Summary: 'This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (E), also known as Beta-1, has multiple differences, one of which causes a frameshift, compared to variant G. It encodes isoform E, which is shorter than isoform G.