NDP (NM_000266) Human Untagged Clone

CAT#: SC120005

NDP (untagged)-Human Norrie disease (pseudoglioma) (NDP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDP
• Synonyms: EVR2; FEVR; ND
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120005 sequence for NM_000266 edited (data generated by NextGen Sequencing)
  ATGAGAAAACATGTACTAGCTGCATCCTTTTCTATGCTCTCCCTGCTGGTGATAATGGGA
  GATACAGACAGTAAAACGGACAGCTCATTCATAATGGACTCGGACCCTCGACGCTGCATG
  AGGCACCACTATGTGGATTCTATCAGTCACCCATTGTACAAGTGTAGCTCAAAGATGGTG
  CTCCTGGCCAGGTGCGAGGGGCACTGCAGCCAGGCGTCACGCTCCGAGCCTTTGGTGTCG
  TTCAGCACTGTCCTCAAGCAACCCTTCCGTTCCTCCTGTCACTGCTGCCGGCCCCAGACT
  TCCAAGCTGAAGGCACTGCGGCTGCGATGCTCAGGGGGCATGCGACTCACTGCCACCTAC
  CGGTACATCCTCTCCTGTCACTGCGAGGAATGCAATTCCTGA
  
  Clone variation with respect to NM_000266.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000266 unedited
  TCAGAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTTAAAC
  AACAGTCCTAACTTTTGTGTGTTGCAAATATAAAAGGCAAGCCATGTGACAGAGGGACAG
  AAGAACAAAAGCATTTGGAAGTAACAGGACCTCTTTCTAGCTCTCAGAAAAGTCTGAGAA
  GAAAGGAGCCCTGCGTTCCCCTAAGCTGTGCAGCAGATACTGTGATGATGGATTGCAAGT
  GCAAAGAGTAAGACAAAACTCCAGCACATAAAGGACAATGACAACCAGAAAGCTTCAGCC
  CGATCCTGCCCTTTCCTTGAACGGGACTGGATCCTAGGAGGTGAAGCCATTTCCAATTTT
  TTGTCCTCTGCCTCCCTCTGCTGTTCTTCTAGAGAAGTTTTTCCTTACAACAATGAGAAA
  ACATGTACTAGCTGCATCCTTTTCTATGCTCTCCCTGCTGGTGATAATGGGAGATACAGA
  CAGTAAAACGGACAGCTCATTCATAATGGACTCGGACCCTCGACGCTGCATGAGGCACCA
  CTATGTGGATTCTATCAGTCACCCATTGTACAAGTGTAGCTCAAAGATGGTGCTCCTGGC
  CAGGTGCGAGGGGCACTGCAGCCAGGCGTCACGCTCCGAGCCTTTGGTGTCGTTCAGCAC
  TGTCCTCAAGCAACCCTTCCGTTCCTCCTGTCACTGCTGCCGGCCCCAGACTTCCNAGCT
  GAAGGCACTGCGGCTGCGATGCTCANGGGGCATGCGACTCACTGCCACCTACCGGTACAT
  CCTCTCCTGTCACTGCGAGGAATGCATTTCCTGAGCCCGCTGCTGTGTGTGGCTTCTGGA
  TGGGACAACTGTAGAGGCAGTCCGACCAGCCAGGGAAAGACTGGGCAGAAAGAGTTAGGG
  CANAAAGGATGCACATTCTNCCGGGACTTGCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000266 unedited
  TGGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAATTACTCTTTT
  TATTTCAAAACGTGGGAATAACTCAAAATTTGAGCCTCCAGGACCAAAACACATTTGAAC
  CAAACGTTCAGAAGACAAGCCAGGCCACCATTTTGGCAACATGTTTCACATGGACAAGTG
  GATATTAGAGAATGATGCCCGTGACACGGCTAAAGCTATAGCACACCCACAAATAGATTC
  ATACTTGGTTGTCTAATTTGCATGCATGAGTATGGTGCATTCTCCATGCCACTAGTACAT
  ATATGCCTTTTCCAGAGTCAGTGCAGGATCCGTATTTGTGCAAGTCTTTAAAAGTCTGCT
  CCCTTACAAAATTTTACCATAATTTATCTGAGACAGCCTTCCTGTGACAAATTCTTTATG
  CTGCTTTTACTTTCAAAAAGTCATTTCTGAATAATAATTATAGCTGTCAAGAGTTCCAGC
  ATCACATAAAAGAATCTGTTTGTTAGTTATTTTAATGTCAGTTCGCTGGCTGTGAGTAAA
  CGTAGCTAAACATATCCACATGCTTGGTATCTGGACTTTGAAATCGGTAACCTTTAGCAG
  CAATGGCAACCTTAGACCAAAATGACAAGGTAAAAAAAAACATCAACAATAACAGAAGAT
  GTCCCAGGAAAAGCTGGGCTTTTCTACTTTCCAAAGTAAATTCTTCCCCAGCGTGCACCA
  AACACTGACAGCCTGACACGCTTGTGTATGAGGGCCCACTNTTTCCCACTAATGCTGAAT
  TGNTGGAAACCCAAACAGCATTGAGAGCCAAGGNGGAAATGCCTNGATCTGAAATGAACC
  AAAGAAATGTACCANAGAAAGGAGAGGGAACTGCAAAGAAGTTCCAGATATCTCTCTCTG
  TCACAGCATGTANAGTCTTATTAN
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_000266
• Insert Size: 1940 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000266.1, NP_000257.1
• RefSeq Size: 1846 bp
• RefSeq ORF: 402 bp
• Locus ID: 4693
• Cytogenetics: Xp11.3
• Domains: CT, Cys_knot
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene encodes a secreted protein with a cystein-knot motif that activates the Wnt/beta-catenin pathway. The protein forms disulfide-linked oligomers in the extracellular matrix. Mutations in this gene result in Norrie disease and X-linked exudative vitreoretinopathy. [provided by RefSeq, Feb 2009]'