PDE6 beta (PDE6B) (NM_000283) Human Untagged Clone

CAT#: SC120018

PDE6B (untagged)-Human phosphodiesterase 6B, cGMP-specific, rod, beta (PDE6B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE6B
• Synonyms: CSNB3; CSNBAD2; GMP-PDEbeta; PDEB; rd1; RP40
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120018 sequence for NM_000283 edited (data generated by NextGen Sequencing)
  ATGAGCCTCAGTGAGGAGCAGGCCCGGAGCTTTCTGGACCAGAACCCCGATTTTGCCCGC
  CAGTACTTTGGGAAGAAACTGAGCCCTGAGAATGTGGCCGCGGCCTGCGAGGACGGGTGC
  CCGCCGGACTGCGACAGCCTCCGGGACCTCTGCCAGGTGGAGGAGAGCACGGCGCTGCTG
  GAGCTGGTGCAGGATATGCAGGAGAGCATCAACATGGAGCGCGTGGTCTTCAAGGTCCTG
  CGGCGCCTCTGCACCCTCCTGCAGGCCGACCGCTGCAGCCTCTTCATGTACCGCCAGCGC
  AACGGCGTGGCCGAGCTGGCCACCAGGCTTTTCAGCGTGCAGCCGGACAGCGTCCTGGAG
  GACTGCCTGGTGCCCCCCGACTCCGAGATCGTCTTCCCACTGGACATCGGGGTCGTGGGC
  CACGTGGCTCAGACCAAAAAGATGGTGAACGTCGAGGACGTGGCCGAGTGCCCTCACTTC
  AGCTCATTTGCTGACGAGCTCACTGACTACAAGACAAAGAATATGCTGGCCACACCCATC
  ATGAATGGCAAAGACGTCGTGGCGGTGATCATGGCAGTGAACAAGCTCAACGGCCCATTC
  TTCACCAGCGAAGACGAAGATGTGTTCTTGAAGTACCTGAATTTTGCCACGTTGTACCTG
  AAGATCTATCACCTGAGCTACCTCCACAACTGCGAGACGCGCCGCGGCCAGGTGCTGCTG
  TGGTCGGCCAACAAGGTGTTTGAGGAGCTGACGGACATCGAGAGGCAGTTCCACAAGGCC
  TTCTACACGGTGCGGGCCTACCTCAACTGCGAGCGGTACTCCGTGGGCCTCCTGGACATG
  ACCAAGGAGAAGGAATTTTTTGACGTGTGGTCTGTGCTGATGGGAGAGTCCCAGCCGTAC
  TCGGGCCCACGCACGCCTGATGGCCGGGAAATTGTCTTCTACAAAGTGATCGACTACATC
  CTCCACGGCAAGGAGGAGATCAAGGTCATTCCCACACCCTCAGCCGATCACTGGGCCCTG
  GCCAGCGGCCTTCCAAGCTACGTGGCAGAAAGCGGCTTTATTTGTAACATCATGAATGCT
  TCCGCTGACGAAATGTTCAAATTTCAGGAAGGGGCCCTGGACGACTCCGGGTGGCTCATC
  AAGAATGTGCTGTCCATGCCCATCGTCAACAAGAAGGAGGAGATTGTGGGAGTCGCCACA
  TTTTACAACAGGAAAGACGGGAAGCCCTTTGACGAACAGGACGAGGTTCTCATGGAGTCC
  CTGACACAGTTCCTGGGCTGGTCAGTGATGAACACCGACACCTACGACAAGATGAACAAG
  CTGGAGAACCGCAAGGACATCGCACAGGACATGGTCCTTTACCACGTGAAGTGCGACAGG
  GACGAGATCCAGCTCATCCTGCCAACCAGAGCGCGCCTGGGGAAGGAGCCTGCTGACTGC
  GATGAGGACGAGCTGGGCGAAATCCTGAAGGAGGAGCTGCCAGGGCCCACCACATTTGAC
  ATCTACGAATTCCACTTCTCTGACCTGGAGTGCACCGAACTGGACCTGGTCAAATGTGGC
  ATCCAGATGTACTACGAGCTGGGCGTGGTCCGAAAGTTCCAGATCCCCCAGGAGGTCCTG
  GTGCGGTTCCTGTTCTCCATCAGCAAAGGGTACCGGAGAATCACCTACCACAACTGGCGC
  CACGGCTTCAACGTGGCCCAGACGATGTTCACGCTGCTCATGACCGGCAAACTGAAGAGC
  TACTACACGGACCTGGAGGCCTTCGCCATGGTGACAGCCGGCCTGTGCCATGACATCGAC
  CACCGCGGCACCAACAACCTGTACCAGATGAAGTCCCAGAACCCCTTGGCTAAGCTCCAC
  GGCTCCTCGATTTTGGAGCGGCACCACCTGGAGTTTGGGAAGTTCCTGCTCTCGGAGGAG
  ACCCTGAACATCTACCAGAACCTGAACCGGCGGCAGCACGATCACGTGATCCACCTGATG
  GACATCGCCATCATCGCCACGGACCTGGCCCTGTACTTCAAGAAGAGAGCGATGTTTCAG
  AAGATCGTGGATGAGTCCAAGAACTACCAGGACAAGAAGAGCTGGGTGGAGTACCTGTCC
  CTGGAGACGACCCGGAAGGAGATCGTCATGGCCATGATGATGACAGCCTGCGACCTGTCT
  GCCATCACCAAGCCCTGGGAAGTCCAGAGCAAGGTCGCACTTCTCGTGGCTGCTGAGTTC
  TGGGAGCAAGGTGACTTGGAAAGGACAGTCTTGGATCAGCAGCCCATTCCTATGATGGAC
  CGGAACAAGGCGGCCGAGCTCCCCAAGCTGCAAGTGGGCTTCATCGACTTCGTGTGCACA
  TTCGTGTACAAGGAGTTCTCTCGTTTCCACGAAGAGATCCTGCCCATGTTCGACCGACTG
  CAGAACAATAGGAAAGAGTGGAAGGCGCTGGCTGATGAGTATGAGGCCAAAGTGAAGGCT
  CTGGAGGAGAAGGAGGAGGAGGAGAGGGTGGCAGCCAAGAAANNAGGCACAGAAATTTGC
  AATGGCGGCCCAGCACCCAAGTCTTCAACCTGCTGTATCCTGTGA
  
  Clone variation with respect to NM_000283.3
  1962 g=>t;2503 g=>n;2504 t=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000283 unedited
  GCCGCNCCTTGGTTTNTTTAAACGATTCATATAGGCGGCGCGCGAATTCGCACGAGGTGC
  GTGCCTGGCACAGCAGCGTCTCCAGGGCAGGCAGCCACCATGAGCCTCAGTGAGGAGCAG
  GCCCGGAGCTTTCTGGACCAGAACCCCGATTTTGCCCGCCAGTACTTTGGGAAGAAACTG
  AGCCCTGAGAATGTGGCCGCGGCCTGCGAGGACGGGTGCCCGCCGGACTGCGACAGCCTC
  CGGGACCTCTGCCAGGTGGAGGAGAGCACGGCGCTGCTGGAGCTGGTGCAGGATATGCAG
  GAGAGCATCAACATGGAGCGCGTGGTCTTCAAGGTCCTGCGGCGCCTCTGCACCCTCCTG
  CAGGCCGACCGCTGCAGCCTCTTCATGTACCGCCAGCGCAACGGCGTGGCCGAGCTGGCC
  ACCAGGCTTTTCAGCGTGCAGCCGGACAGCGTCCTGGAGGACTGCCTGGTGCCCCCCGAC
  TCCGAGATCGTCTTCCCACTGGACATCGGGGTCGTGGGCCACGTGGCTCAGACCAAAAAG
  ATGGTGAACGTCGAGGACGTGGCCGAGTGCCCTCACTTCAGCTCATTTGCTGACGAGCTC
  ACTGACTACAAGACAAAGAATATGCTGGCCACACCCATCATGAATGGCAAAGACGTCGTG
  GCGGTGATCATGGCAGTGAACAAGCTCAACGGCCCATTCTTCACCAGCGAAGACGAAGAT
  GTGTTCTTGAAGTACCTGAATTTTGCCACGTTGTACCTGGAGATCTATCACCTGAGCTAC
  CTNCACAACTGCGAGACGCGCCGCGGCCCAGTGCTGCTGTGGGTCGCCAACAAGGTGTTT
  TGAGAGCTGACGGACATCGAGAGGCAGTTCCACAGGCCTTCTACACGGTG
  
• Restriction Sites: Please inquire
• ACCN: NM_000283
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000283.1, NP_000274.1
• RefSeq Size: 3231 bp
• RefSeq ORF: 2565 bp
• Locus ID: 5158
• Cytogenetics: 4p16.3
• Domains: PDEase, GAF, HDc
• Protein Families: Druggable Genome
• Gene Summary: 'Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).