LCAT (NM_000229) Human Untagged Clone

CAT#: SC120019

LCAT (untagged)-Human lecithin-cholesterol acyltransferase (LCAT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LCAT
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120019 sequence for NM_000229 edited (data generated by NextGen Sequencing)
  ATGGGGCCGCCCGGCTCCCCATGGCAGTGGGTGACGCTGCTGCTGGGGCTGCTGCTCCCT
  CCTGCCGCCCCCTTCTGGCTCCTCAATGTGCTCTTCCCCCCGCACACCACGCCCAAGGCT
  GAGCTCAGTAACCACACACGGCCCGTCATCCTCGTGCCCGGCTGCCTGGGGAATCAGCTA
  GAAGCCAAGCTGGACAAACCAGATGTGGTGAACTGGATGTGCTACCGCAAGACAGAGGAC
  TTCTTCACCATCTGGCTGGATCTCAACATGTTCCTACCCCTTGGGGTAGACTGCTGGATC
  GATAACACCAGGGTTGTCTACAACCGGAGCTCTGGGCTCGTGTCCAACGCCCCTGGTGTC
  CAGATCCGCGTCCCTGGCTTTGGCAAGACCTACTCTGTGGAGTACCTGGACAGCAGCAAG
  CTGGCAGGGTACCTGCACACACTGGTGCAGAACCTGGTCAACAATGGCTACGTGCGGGAC
  GAGACTGTGCGCGCCGCCCCCTATGACTGGCGGCTGGAGCCCGGCCAGCAGGAGGAGTAC
  TACCGCAAGCTCGCAGGGCTGGTGGAGGAGATGCACGCTGCCTATGGGAAGCCTGTCTTC
  CTCATTGGCCACAGCCTCGGCTGTCTACACTTGCTCTATTTCCTGCTGCGCCAGCCCCAG
  GCCTGGAAGGACCGCTTTATTGATGGCTTCATCTCTCTTGGGGCTCCCTGGGGTGGCTCC
  ATCAAGCCCATGCTGGTCTTGGCCTCAGGTGACAACCAGGGCATCCCCATCATGTCCAGC
  ATCAAGCTGAAAGAGGAGCAGCGCATAACCACCACCTCCCCCTGGATGTTTCCCTCTCGC
  ATGGCGTGGCCTGAGGACCACGTGTTCATTTCCACACCCAGCTTCAACTACACAGGCCGT
  GACTTCCAACGCTTCTTTGCAGACCTGCACTTTGAGGAAGGCTGGTACATGTGGCTGCAG
  TCACGTGACCTCCTGGCAGGACTCCCAGCACCTGGTGTGGAAGTATACTGTCTTTACGGC
  GTGGGCCTGCCCACGCCCCGCACCTACATCTACGACCACGGCTTCCCCTACACGGACCCT
  GTGGGTGTGCTCTATGAGGATGGTGATGACACGGTGGCGACCCGCAGCACCGAGCTCTGT
  GGCCTGTGGCAGGGCCGCCAGCCACAGCCTGTGCACCTGCTGCCCCTGCACGGGATACAG
  CATCTCAACATGGTCTTCAGCAACCTGACCCTGGAGCACATCAATGCCATCCTGCTGGGT
  GCCTACCGCCAGGGTCCCCCTGCATCCCCGACTGCCAGCCCAGAGCCCCCGCCTCCTGAA
  TAA
  
  Clone variation with respect to NM_000229.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000229 unedited
  TATCCCCCGCCCCTTGCCGCATAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC
  AGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGA
  ATTCGGCACCAGGGAATGGGGCCGCCCGGCTCCCCATGGCAGTGGGTGACGCTGCTGCTG
  GGGCTGCTGCTCCCTCCTGCCGCCCCCTTCTGGCTCCTCAATGTGCTCTTCCCCCCGCAC
  ACCACGCCCAAGGCTGAGCTCAGTAACCACACACGGCCCGTCATCCTCGTGCCCGGCTGC
  CTGGGGAATCAGCTAGAAGCCAAGCTGGACAAACCAGATGTGGTGAACTGGATGTGCTAC
  CGCAAGACAGAGGACTTCTTCACCATCTGGCTGGATCTCAACATGTTCCTACCCCTTGGG
  GTAGACTGCTGGATCGATAACACCAGGGTTGTCTACAACCGGAGCTCTGGGCTCGTGTCC
  AACGCCCCTGGTGTCCAGATCCGCGTCCCTGGCTTTGGCAAGACCTACTCTGTGGAGTAC
  CTGGACAGCAGCAAGCTGGCAGGGTACCTGCACACACTGGTGCAGAACCTGGTCAACAAT
  GGCTACGTGCGGGACGAGACTGTGCGCGCCGCCCCCTATGACTGGCGGCTGGAGCCCGGC
  CAGCAGGAGGAGTACTACCGCAAGCTCGCAGGGCTGGTGGAGGAGATGCACGCTGCCTAT
  GGGAAGCCTGTNCTCCTCATTGGCCACAGCCTCGGCTGTCTACACTTGCTCTATTTCCTG
  CTGCGCCAGCCCCAGGCCTGGAAGGACCGCTTTATTGATGGCTTCATCTCTCTTGGGGCT
  CCCTGGGGTGGCTNCATCAAGCCCATGCTGGTCTTGGCCTCAGTGACAACCAGGCATCCC
  ATCATGTCCAGCATCAAGCTGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000229 unedited
  NNNAATTAGCTTGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTAC
  GGTAGCAAAGGAAGGTCTTTATTCAGGAGGCGGGGGCTCTGGGCTGGCAGTCGGGGATGC
  AGGGGGACCCTGGCGGTAGGCACCCAGCAGGATGGCATTGATGTGCTCCAGGGTCAGGTT
  GCTGAAGACCATGTTGAGATGCTGTATCCCGTGCAGGGGCAGCAGGTGCACAGGCTGTGG
  CTGGCGGCCCTGCCACAGGCCACAGAGCTCGGTGCTGCGGGTCGCCACCGTGTCATCACC
  ATCCTCATAGAGCACACCCACAGGGTCCGTGTAGGGGAAGCCGTGGTCGTAGATGTAGGT
  GCGGGGGCGTGGNCAGGCCCACGCCGTAAAGACAGTATACTTCCACACCAGGTGCTGGGA
  GTCCTGCCAGGAGGTCACGTGACTGCAGCCACATGTACCAGCCTTCCTCAAAGTGCAGGT
  CTGCAAAGAAGCGTTGGAAGTCACGGCCTGTGTAGTTGAAGCTGGGTGTGGAAATGAACA
  CGTGGTCCTCAAGCCACGCCATGCGAGAGGGAAACATCCAGGGGGAGGTGGTGGTTATGC
  GCTGCTCCTCTTTCAGCTTGATGCTGGACATGATGGGGATGCCCTGGTTGTCACCTGAAG
  CCAAGACCAGCATGGGCTTGATGGACCCACCCCAGGGAGCCCCAAGAGAGATGAAGCCAT
  CAATAAAGCGGTCCTTCAGGCCTGGGGCTGGCGCAACAGGAAATAGAGCAAGTGTAGACA
  GCCCGAGGCTGTGGCCCCATGAAGAAGAAAAGGCTTCCCATAGACAGCGTGCAATTTTCC
  TTCAACCACCCTGCGAGCTTTGGGGAAGTACTCCCTTCTGCTGGCCCGGGCTCAACCCGC
  CAGTCCTAAGGAGGCG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_000229
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000229.1, NP_000220.1
• RefSeq Size: 1354 bp
• RefSeq ORF: 1323 bp
• Locus ID: 3931
• Cytogenetics: 16q22.1
• Domains: LACT
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycerophospholipid metabolism
• Gene Summary: 'This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008]'