beta Sarcoglycan (SGCB) (NM_000232) Human Untagged Clone

CAT#: SC120022

SGCB (untagged)-Human sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) (SGCB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SGCB
• Synonyms: A3b; LGMD2E; LGMDR4; SGC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000232, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCAGCGGCGGCGGCGGCTGCAGAACAGCAAAGTTCCAATGGTCCTGTAAAGAAGTCCATGCGTG
  AGAAGGCTGTTGAGAGAAGGAGTGTCAATAAAGAGCACAACAGTAACTTTAAAGCTGGATACATTCCGAT
  TGATGAAGATCGTCTCCACAAAACAGGGTTGAGAGGAAGAAAGGGCAATTTAGCCATCTGTGTGATTATC
  CTCTTGTTTATCCTGGCTGTCATCAATTTAATAATAACACTTGTTATTTGGGCCGTGATTCGCATTGGAC
  CAAATGGCTGTGATAGTATGGAGTTTCATGAAAGTGGCCTGCTTCGATTTAAGCAAGTATCTGACATGGG
  AGTGATCCACCCTCTTTATAAAAGCACAGTAGGAGGAAGGCGAAATGAAAATTTGGTCATCACTGGCAAC
  AACCAGCCTATTGTTTTTCAGCAAGGGACAACAAAGCTCAGTGTAGAAAACAACAAAACTTCTATTACAA
  GTGACATCGGCATGCAGTTTTTTGACCCGAGGACTCAAAATATCTTATTCAGCACAGACTATGAAACTCA
  TGAGTTTCATTTGCCAAGTGGAGTGAAAAGTTTGAATGTTCAAAAGGCATCTACTGAAAGGATTACCAGC
  AATGCTACCAGTGATTTAAATATAAAAGTTGATGGGCGTGCTATTGTGCGTGGAAATGAAGGTGTATTCA
  TTATGGGCAAAACCATTGAATTTCACATGGGTGGTAATATGGAGTTAAAGGCGGAAAACAGTATCATCCT
  AAATGGATCTGTGATGGTCAGCACCACCCGCCTACCCAGTTCCTCCAGTGGAGACCAGTTGGGTAGTGGT
  GACTGGGTACGCTACAAGCTCTGCATGTGTGCTGATGGGACGCTCTTCAAGGTGCAAGTAACCAGCCAGA
  ACATGGGCTGCCAAATCTCAGACAACCCCTGTGGAAACACTCATTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000232 unedited
  TTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGGGAAGATGGCGGCA
  GCGGCGGCGGCGGCTGCAGAACAGCAAAGTTCCAATGGTCCTGTAAAGAAGTCCATGCGT
  GAGAAGGCTGTTGAGAGAAGGAGTGTCAATAAAGAGCACAACAGTAACTTTAAAGCTGGA
  TACATTCCGATTGATGAAGATCGTCTCCACAAAACAGGGTTGAGAGGAAGAAAGGGCAAT
  TTAGCCATCTGTGTGATTATCCTCTTGTTTATCCTGGCTGTCATCAATTTAATAATAACA
  CTTGTTATTTGGGCCGTGATTCGCATTGGACCAAATGGCTGTGATAGTATGGAGTTTCAT
  GAAAGTGGCCTGCTTCGATTTAAGCAAGTATCTGACATGGGAGTGATCCACCCTCTTTAT
  AAAAGCACAGTAGGAGGAAGGCGAAATGAAAATTTGGTCATCACTGGCAACAACCAGCCT
  ATTGTTTTTCAGCAAGGGACAACAAAGCTCAGTGTAGAAAACAACAAAACTTCTATTACA
  AGTGACATCGGCATGCAGTTTTTTGACCCGAGGACTCAAAATATCTTATTCAGCACAGAC
  TATGAAACTCATGAGTTTCATTTGCCAAGTGGAGTGAAAAGTTTGAATGTTCAAAAGGCA
  TCTACTGAAAGGGATTACCAGCATGCTACCAGTGATTTAATATAAAAGTGATNGGGCGTG
  CTATNGGTGCGTGGAAATGAANGGTGTATTCATTATGGGCAAAACCATTGAATTTACATG
  GNGTGGTATNNATGGAGTTAAAGGCGGNAAACAGTATCATCTAAATGGATCTGTGATGGT
  CAGCACCACCCGCTACCCANTNNNCTCATGAGACANNTGGGGNANTGGGGACTTGGGACC
  TACAGCTCTGGATGGTGCTGATGGGACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000232 unedited
  CCGCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAATTTACAGAATTTTT
  ATTGTAAACAGAAGCTCATTACTAGTTATTACAAACTGAATCAACTGAAACTCAAGTTAG
  CAATGTGAAACACATTTATAGGCTTATTTTTGTATACCATAATTGGTAATTTTTGCCCCC
  TCAATACTTTTAAAAAGACTGTTAAAATTTTCAGCATTTCAATGATCTCTTATTTGTCAG
  GTTTAGCACCATGACATTCTCATTCCCTTAAAAAAACACTCATTCAAATCTAGGTAGAAC
  TTCCACCTTTCATCATCAAAATTATCACACAAAATTTATTTTGTTTTTTTCACAATGCAA
  AAAGAAGACAAATAAGTATATTTTAAAATATTATCAGCAGTCCGTTTTGTGTGGTTTTAC
  ATTTTTCTGTCTGCCCATTATTAAAAAATCTTGCATTGATTTCATTTGCCCCCCTTGTCA
  TTGCAACCCGTCTCTAGAATACTGTGGTAATTGAAACTAGCATGTACCTGCGCCCCCTCC
  CGGGGTCCTATCTTTGTCACCCACCTCCCCCATGGCTACTGCTTGTCATATCTGTGCTCA
  GTGCAGGGATGAGCATACAGCAGGAAGAGGCGAAGTCAGCATTTCATTTACAGAAAAATA
  CTATCTGGTGGAGACAATGAGTTGCTGAAGATTTACTGTGGATAAAAATAAGCCGGCCAG
  GCGTGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGCAGATCACGA
  TGTCAAGAGATTGAGACCATCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATAC
  AAAAATTANCCCGGGACTGTGGCACATGCCTGTAGTCCCAGTACTCGGGAAGGCTGAGCA
  GAGAATCGCTTGACCCCTGGAGCANAGNTGCATGATCCANAGCGATTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000232
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000232.3, NP_000223.1
• RefSeq Size: 4229 bp
• RefSeq ORF: 957 bp
• Locus ID: 6443
• Cytogenetics: 4q12
• Domains: sarcoglycan
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), Viral myocarditis
• Gene Summary: 'This gene encodes a member of the sarcoglycan family. Sarcoglycans are transmembrane components in the dystrophin-glycoprotein complex which help stabilize the muscle fiber membranes and link the muscle cytoskeleton to the extracellular matrix. Mutations in this gene have been associated with limb-girdle muscular dystrophy.[provided by RefSeq, Oct 2008]'