Hemoglobin subunit gamma 2 (HBG2) (NM_000184) Human Untagged Clone

CAT#: SC120038

HBG2 (untagged)-Human hemoglobin, gamma G (HBG2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HBG2
• Synonyms: HBG-T1; TNCY
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120038 sequence for NM_000184 edited (data generated by NextGen Sequencing)
  ATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT
  GTGGAAGATGCTGGAGGAGAAACCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAG
  AGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCTCTGCCATCATGGGCAACCCCAAA
  GTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGAT
  GATCTCAAGGGCACCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGAT
  CCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGC
  AAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGGAGTGGCCAGT
  GCCCTGTCCTCCAGATACCACTGA
  
  Clone variation with respect to NM_000184.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000184 unedited
  GAAGCCTGGGTTCTTTCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGG
  TGAATGTGGAAGATGCTGGAGGAGAAACCCTGGGAAAGGCTCCTGGTTGTCTACCCATGG
  ACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCTCTGCCATCATGGGCAAC
  CCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCAC
  CTGGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCAT
  GTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCAT
  TTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGGAGTG
  GCCAGTGCCCTGTCCTCCAGATACCACTGAGCTCACTGCCCATGATGCAGAGCTTTCAAG
  GATAGGCTTTATTCTGCAAGCAATCAAATAATAAATCTATTCTGCTAAGAGATCACAAAA
  AAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACA
  GATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCC
  ACTCCAGTGCCCACCAGCCTTGTCCTATAAAATTAAGTTGCATCATTTTGTCTGACTAGG
  TGTCCTTCTTATATATGGGTGAGGGGGGGNGNGGNGNNTNNNNANNN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000184 unedited
  NTTAGCTCTGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTGGG
  ATCTCTTAGCAGAATAGATTTATTATTTGATTGCTTGCAGAATAAAGCCTATCCTTGAAA
  GCTCTGCATCATGGGCAGTGAGCTCAGTGGTATCTGGAGGACAGGGCACTGGCCACTCCA
  GTCACCATCTTCTGCCAGGAAGCCTGCACCTCAGGGGTGAATTCTTTGCCGAAATGGATT
  GCCAAAACGGTCACCAGCACATTTCCCAGGAGCTTGAAGTTCTCAGGATCCACATGCAGC
  TTGTCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTT
  ATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCC
  ATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTCTGGGTCCATGGG
  TAGACAACCAGGAGCCTTCCCAGGGTTTCTCCTCCAGCATCTTCCACATTCACCTTGCCC
  CACAGGCTTGTGATAGTAGCCTTGTCCTCCTCTGTGAAATGACCCATGGCGTCTGGACTA
  GGAGCTTATTGATAACCTCAGCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTA
  TTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACAC
  GCCTACCGNCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTTGGAAAGTNCCGTT
  GATTTTGGTGCCAAACAAACTTCCATTGACGTCATGGGGGTGGGAGACTTGGAAATCCCC
  GTGAGTCAAACCGCTATCCACGCCCATTGGTGTACTGGCCAAACCGCATCACATGGTATT
  AGCGAGACTAATCCCTANATGTACTGCAGTNAGGAAGTCCCGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000184
• Insert Size: 620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000184.2, NP_000175.1
• RefSeq Size: 583 bp
• RefSeq ORF: 444 bp
• Locus ID: 3048
• Cytogenetics: 11p15.4
• Domains: globin
• Gene Summary: 'The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'- epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008]'