ICAM1 (NM_000201) Human Untagged Clone

CAT#: SC120049

ICAM1 (untagged)-Human intercellular adhesion molecule 1 (ICAM1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ICAM1
• Synonyms: BB2; CD54; P3.58
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000201 edited
  GAATTCGGCACGAGGCAGAGTTGCAACCTCAGCCTCGCTATGGCTCCCAGCAGCCCCCGG
  CCCGCGCTGCCCGCACTCCTGGTCCTGCTCGGGGCTCTGTTCCCAGGACCTGGCAATGCC
  CAGACATCTGTGTCCCCCTCAAAAGTCATCCTGCCCCGGGGAGGCTCCGTGCTGGTGACA
  TGCAGCACCTCCTGTGACCAGCCCAAGTTGTTGGGCATAGAGACCCCGTTGCCTAAAAAG
  GAGTTGCTCCTGCCTGGGAACAACCGGAAGGTGTATGAACTGAGCAATGTGCAAGAAGAT
  AGCCAACCAATGTGCTATTCAAACTGCCCTGATGGGCAGTCAACAGCTAAAACCTTCCTC
  ACCGTGTACTGGACTCCAGAACGGGTGGAACTGGCACCCCTCCCCTCTTGGCAGCCAGTG
  GGCAAGAACCTTACCCTACGCTGCCAGGTGGAGGGTGGGGCACCCCGGGCCAACCTCACC
  GTGGTGCTGCTCCGTGGGGAGAAGGAGCTGAAACGGGAGCCAGCTGTGGGGGAGCCCGCT
  GAGGTCACGACCACGGTGCTGGTGAGGAGAGATCACCATGGAGCCAATTTCTCGTGCCGC
  ACTGAACTGGACCTGCGGCCCCAAGGGCTGGAGCTGTTTGAGAACACCTCGGCCCCCTAC
  CAGCTCCAGACCTTTGTCCTGCCAGCGACTCCCCCACAACTTGTCAGCCCCCGGGTCCTA
  GAGGTGGACACGCAGGGGACCGTGGTCTGTTCCCTGGACGGGCTGTTCCCAGTCTCGGAG
  GCCCAGGTCCACCTGGCACTGGGGGACCAGAGGTTGAACCCCACAGTCACCTATGGCAAC
  GACTCCTTCTCGGCCAAGGCCTCAGTCAGTGTGACCGCAGAGGACGAGGGCACCCAGCGG
  CTGACGTGTGCAGTAATACTGGGGAACCAGAGCCAGGAGACACTGCAGACAGTGACCATC
  TACAGCTTTCCGGCGCCCAACGTGATTCTGACGAAGCCAGAGGTCTCAGAAGGGACCGAG
  GTGACAGTGAAGTGTGAGGCCCACCCTAGAGCCAAGGTGACGCTGAATGGGGTTCCAGCC
  CAGCCACTGGGCCCGAGGGCCCAGCTCCTGCTGAAGGCCACCCCAGAGGACAACGGGCGC
  AGCTTCTCCTGCTCTGCAACCCTGGAGGTGGCCGGCCAGCTTATACACAAGAACCAGACC
  CGGGAGCTTCGTGTCCTGTATGGCCCCCGACTGGACGAGAGGGATTGTCCGGGAAACTGG
  ACGTGGCCAGAAAATTCCCAGCAGACTCCAATGTGCCAGGCTTGGGGGAACCCATTGCCC
  GAGCTCAAGTGTCTAAAGGATGGCACTTTCCCACTGCCCATCGGGGAATCAGTGACTGTC
  ACTCGAGATCTTGAGGGCACCTACCTCTGTCGGGCCAGGAGCACTCAAGGGGAGGTCACC
  CGCAAGGTGACCGTGAATGTGCTCTCCCCCCGGTATGAGATTGTCATCATCACTGTGGTA
  GCAGCCGCAGTCATAATGGGCACTGCAGGCCTCAGCACGTACCTCTATAACCGCCAGCGG
  AAGATCAAGAAATACAGACTACAACAGGCCCAAAAAGGGACCCCCATGAAACCGAACACA
  CAAGCCACGCCTCCCTGAACCTATCCCGGGACAGGGCCTCTTCCTCGGCCTTCCCATATT
  GGTGGCAGTGGTGCCACACTGAACAGAGTGGAAGACATATGCCATGCAGCTACACCTACC
  GGCCCTGGGACGCCGGAGGACAGGGCATTGTCCTCAGTCAGATACAACAGCATTTGGGGC
  CATGGTACCTGCACACCTAAAACACTAGGCCACGCATCTGATCTGTAGTCACATGACTAA
  GCCAAGAGGAAGGAGCAAGACTCAAGACATGATTGATGGATGTTAAAGTCTAGCCTGATG
  AGAGGGGAAGTGGTGGGGGAGACATAGCCCCACCATGAGGACATACAACTGGGAAATACT
  GAAACTTGCTGCCTATTGGGTATGCTGAGGCCCCACAGACTTACAGAAGAAGTGGCCCTC
  CATAGACATGTGTAGCATCAAAACACAAAGGCCCACACTTCCTGACGGATGCCAGCTTGG
  GCACTGCTGTCTACTGACCCCAACCCTTGATGATATGTATTTATTCATTTGTTATTTTAC
  CAGCTATTTATTGAGTGTCTTTTATGTAGGCTAAATGAACATAGGTCTCTGGCCTCACGG
  AGCTCCCAGTCCTAATCACATTCAAGGTCACCAGGTACAGTTGTACAGGTTGTACACTGC
  AGGAGAGTGCCTGGCAAAAAGATCAAATGGGGCTGGGACTTCTCATTGGCCAACCTGCCT
  TTCCCCAGAAGGAGTGATTTTTCTATCGGCACAAAAGCACTATATGGACTGGTAATGGTT
  ACAGGTTCAGAGATTACCCAGTGAGGCCTTATTCCTCCCTTCCCCCCAAAACTGACACCT
  TTGTTAGCCACCTCCCCACCCACATACATTTCTGCCAGTGTTCACAATGACACTCAGCGG
  TCATGTCTGGACATGAGTGCCCAGGGAATATGCCCAAGCTATGCCTTGTCCTCTTGTCCT
  GTTTGCATTTCACTGGGAGCTTGCACTATGCAGCTCCAGTTTCCTGCAGTGATCAGGGTC
  CTGCAAGCAGTGGGGAAGGGGGCCAAGGTATTGGAGGACTCCCTCCCAGCTTTGGAAGCC
  TCATCCGCGTGTGTGTGTGTGTGTATGTGTAGACAAGCTCTCGCTCTGTCACCCAGGCTG
  GAGTGCAGTGGTGCAATCATGGTTCACTGCAGTCTTGACCTTTTGGGCTCAAGTGATCCT
  CCCACCTCAGCCTCCTGAGTAGCTGGGACCATAGGCTCACAACACCACACCTGGCAAATT
  TGATTTTTTTTTTTTTTCCAGAGACGGGGTCTCGCAACATTGCCCAGACTTCCTTTGTGT
  TAGTTAATAAAGCTTTCTCAACTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACT
  CGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000201 unedited
  NGTTCACAAATTGTATACGATTCACTATAGGCGGCCGCGAATTCGCACGAGGCAGAGTTG
  CAACCTCAGCCTCGCTATGGCTCCCAGCAGCCCCCGGCCCGCGCTGCCCGCACTCCTGGT
  CCTGCTCGGGGCTCTGTTCCCAGGACCTGGCAATGCCCAGACATCTGTGTCCCCCTCAAA
  AGTCATCCTGCCCCGGGGAGGCTCCGTGCTGGTGACATGCAGCACCTCCTGTGACCAGCC
  CAAGTTGTTGGGCATAGAGACCCCGTTGCCTAAAAAGGAGTTGCTCCTGCCTGGGAACAA
  CCGGAAGGTGTATGAACTGAGCAATGTGCAAGAAGATAGCCAACCAATGTGCTATTCAAA
  CTGCCCTGATGGGCAGTCAACAGCTAAAACCTTCCTCACCGTGTACTGGACTCCAGAACG
  GGTGGAACTGGCACCCCTCCCCTCTTGGCAGCCAGTGGGCAAGAACCTTACCCTACGCTG
  CCAGGTGGAGGGTGGGGCACCCCGGGCCAACCTCACCGTGGTGCTGCTCCGTGGGGAGAA
  GGAGCTGAAACGGGAGCCAGCTGTGGGGGAGCCCGCTGAGGTCACGACCACGGTGCTGGT
  GAGGAGAGATCACCATGGAGCCAATTTCTCGTGCCGCACTGAACTGGACCTGCGGCCCCA
  AGGGCTGGAGCTGTTTGAGAACACCTCGGCCCCCTACCAGCTCCAGACCTTTGTCCTGCC
  AGCGACTCCCCCACAACTTGTCAGCCCCCGGGTCCTAGAGGTGGACACGCANGGGACCGT
  GGTCTGTTCCCTGGACGGGCTGTTCCCAGTCTCGGAGCCCAGGTCACCTTGCACTGGGGG
  ACCAGAGTTGAACCCCCAGTCACCTATGCAACGACTCCTTTTCGCCAAGCCCTAGTCATG
  TC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000201 unedited
  GTAACGCGGTCCGCAATTCTATAGTCGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTGCGAGCGAGAAAGCTTTATTAACTAACACAAAGGAAGTCTGGGCAATGTTGCGAGACC
  CCGTCTCTGGAAAAAAAAAAAAAATCAAATTTGCCAGGGGGGGGGTTGTGAGCCTATGGT
  CCCAGCTACTCAAGAGGCTGAGGGGGGAGGATCACTTGAGCCCAAAAGGGCAAGACTGCA
  GTGAACCATGATTGCACCACTGGACTCCAGCCTGGGTGACAGAGCGAGAGCTTGTCTACA
  CATACACACACACACACACGCGGATGAGGCTTCCAAAGCTGGGAGGGAGTCCTCCAATAC
  CTTGGCCCCCTTCCCCACTGCTTGCAGGACCCTGATCACTGCAGGAAACTGGAGCTGCAT
  AGTGCAAGCTCCCAGCGAAATGCAAACAGGACAAGAGGACAAGGCATAGCTTGGGCATAT
  TCCCTGGGCACTTATGTCCAGACATGACCGCTGAGTGTCATTGTGAACACTGGCATAAAT
  GTTTGTGGGTGGGGAGGTGGCTAACAAAAGTGTGAGTTTTGGGGGGAAGGGAGGAATAAG
  GGCTTACTGGGTAATCTCTGAACCTGTAACCTTACCAGNCCATATAGTGCCTTTTGGCCG
  ATAGAAAATCACTCCTTCTGGAGAAAGGCAGGTTGGCCAATGAGAATTCCCAACCCCATT
  TGTTCTTTTGTCAGGCACTCTCCTGCAGGGACCACCTGGACAACTGGCCGGTGACCTTGA
  ATGGGATAAGACTGGGACCCCGGGAAGCCACAACCCATGTNCATTTAACCACCTAAAAAC
  CTCCTAATATGCGGGAAATAACATGAATAATCCTATTTCTAAGGTTGGGGCAATTAAAAG
  ATGGCCAATCTGGTTCCCCAGAAGAGGGGCCCTGGGGTTGAAGCCAAATGTTTAGGAGGC
  CCTCTTCTTTATGTCGGGGCTCAAAATACATAGGAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000201
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000201.1, NP_000192.1
• RefSeq Size: 2986 bp
• RefSeq ORF: 1599 bp
• Locus ID: 3383
• Cytogenetics: 19p13.2
• Domains: IG, ICAM_N
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Viral myocarditis
• Gene Summary: 'This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]'